Abstract:
:In this work, we disrupted one of three putative phosphatidylinositol phospholipase C genes of Aspergillus nidulans and studied its effect on carbon source sensing linked to vegetative mitotic nuclear division. We showed that glucose does not affect nuclear division rates during early vegetative conidial germination (6-7 h) in either the wild type or the plcA-deficient mutant. Only after 8 h of cultivation on glucose did the mutant strain present some decrease in nuclear duplication. However, decreased nuclear division rates were observed in the wild type when cultivated in media amended with polypectate, whereas our plcA-deficient mutant did not show slow nuclear duplication rates when grown on this carbon source, even though it requires induction and secretion of multiple pectinolytic enzymes to be metabolized. Thus, plcA appears to be directly linked to high-molecular-weight carbon source sensing.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Vanzela AP,Said S,Prade RAdoi
10.1139/w11-034subject
Has Abstractpub_date
2011-07-01 00:00:00pages
611-6issue
7eissn
0008-4166issn
1480-3275journal_volume
57pub_type
杂志文章abstract::The induction of antituberculous immunity by crude ribosomal fractions isolated from Mycobacterium tuberculosis strain H37Ra, M. bovis strain BCG, and M. smegmatis was studied in CF-1 mice. Levels of antituberculous immunity similar to that induced by live BCG were induced by the BCG and H37Ra ribosomal fractions wher...
journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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