Reconstitution of glucose uptake and chemotaxis in Pseudomonas aeruginosa glucose transport defective mutants.

Abstract:

:Wild-type glucose uptake and glucose chemotaxis activities were restored in glucose transport defective Pseudomonas aeruginosa strains PFB360 and PFB362 after introduction of plasmid pPZ129, containing a 1.1-kilobase DNA fragment that is essential for the expression of the P. aeruginosa periplasmic glucose binding protein. The restoration of glucose uptake and chemotaxis to wild-type levels in these strains was also achieved by reconstitution with cold-shock fluid and purified glucose binding protein isolated from P. aeruginosa PA01 wild-type strain H103 grown in conditions resulting in the induction of the high-affinity glucose transport system. Glucose uptake was determined by whole cell uptake and shock fluid binding of D-[U-14C]glucose, using standard filter binding assays. Positive chemotaxis towards glucose was assessed by capillary assays using 10 mM glucose, the amount required for optimal chemotaxis, and judged by plating capillary contents accumulated after 30 min.

journal_name

Can J Microbiol

authors

Sly LM,Worobec EA,Perkins RE,Phibbs PV Jr

doi

10.1139/m93-163

subject

Has Abstract

pub_date

1993-11-01 00:00:00

pages

1079-83

issue

11

eissn

0008-4166

issn

1480-3275

journal_volume

39

pub_type

杂志文章
  • Bindings of plasma proteins to streptococci of serological group L with special reference to their immunoglobulin G Fc-receptor activity.

    abstract::Of 33 streptococcal cultures belonging to serological group L, all bound human immunoglobulin (Ig) G, fibrinogen, and fibronectin; 32 bound bovine IgG; 31 bound alpha 2-macroglobulin; 5 bound albumin; and none bound either haptoglobin or IgA. The binding sites for IgG could be isolated from the L streptococci by tryps...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-001

    authors: Lämmler C,Schaufuss P,Frede C,Blobel H

    更新日期:1988-01-01 00:00:00

  • Construction and preliminary characterization of a nondefective herpes simplex virus recombinant bearing the genome of human papillomavirus type 16.

    abstract::Studies of the molecular biology of human papillomavirus type 16 have been limited by the lack of a tissue culture system that is fully permissive for virus replication; as a result, high-titre stocks of infectious virus are not readily available. Therefore, studies of viral gene expression have relied on analysis of ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m93-016

    authors: Salloukh H,Lavery C,Smiley JR,Rawls WE

    更新日期:1993-01-01 00:00:00

  • A multiple extraction--centrifugation method for the recovery of viruses from waste water treatment plant effluents and sludges.

    abstract::Absorption of 14C-labelled poliovirus-2 to sedimentable solids of primary sludge samples collected from a secondary treatment facility during a 6-month period averaged 94%; for anaerobically digested sludge, 99%. The extent of virus adsorption was influenced by the amount of solids. Maximal adsorption occurred at or a...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-254

    authors: Lewis MA,Nath MW,Johnson JC

    更新日期:1983-12-01 00:00:00

  • Modification of the protein expression pattern induced in the nitrogen-fixing actinomycete Frankia sp. strain ACN14a-tsr by root exudates of its symbiotic host Alnus glutinosa and cloning of the sodF gene.

    abstract::Two-dimensional (2-D) polyacrylamide gel electrophoresis was used to detect proteins induced in Frankia sp. strain ACN14a-tsr by root exudates of its symbiotic host, Alnus glutinosa. The 5 most prominent proteins were purified from 2-D gels and characterized by N-terminal sequencing. All of these proteins had a high p...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w01-046

    authors: Hammad Y,Maréchal J,Cournoyer B,Normand P,Domenach AM

    更新日期:2001-06-01 00:00:00

  • A N2-fixing endophytic Burkholderia sp. associated with maize plants cultivated in Mexico.

    abstract::In the frame of a survey of potentially endophytic N2-fixing Burkholderia associated with maize in Mexico, its country of origin, the soil of an indigenous maize field near Oaxaca was studied. Under laboratory conditions, plant seedlings of two ancient maize varieties were used as a trap to select endophyte candidates...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w02-023

    authors: Estrada P,Mavingui P,Cournoyer B,Fontaine F,Balandreau J,Caballero-Mellado J

    更新日期:2002-04-01 00:00:00

  • Mapping of genes encoding glycoside hydrolases on the chromosome of Cellulomonas fimi.

    abstract::Cellulomonas fimi genomic DNA was digested with HpaI, MunI, HindIII, and NsiI, producing fragments ranging in size from 20 to 1400 kbp that were resolved by pulsed field gel electrophoresis. Genetic and physical linkages were determined by Southern blotting and were used to construct a genome map. Cellulomonas fimi ha...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Stoll D

    更新日期:2001-12-01 00:00:00

  • Spoligotype analysis of Mycobacterium bovis isolates from Northern México.

    abstract::Bovine tuberculosis is still rife in Latin America, producing huge economic losses. There are very few studies of the way this disease is spread through this geographical region, particularly in countries that border those that are almost free of Mycobacterium bovis. In this work, we have analyzed the spacer oligonucl...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w05-083

    authors: Cobos-Marín L,Montes-Vargas J,Zumarraga M,Cataldi A,Romano MI,Estrada-Garcia I,Gonzalez-y-Merchand JA

    更新日期:2005-11-01 00:00:00

  • Subcellular localization of the Streptococcus mutans P1 protein C terminus.

    abstract::To determine the subcellular location of the Streptococcus mutans P1 protein C-terminal anchor, cell envelope fractionation experiments were conducted in combination with Western immunoblotting, using monoclonal antibody MAb 6-8C specific for an epitope that maps near the C terminus of P1 protein and also a polyclonal...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Homonylo-McGavin MK,Lee SF,Bowden GH

    更新日期:1999-06-01 00:00:00

  • Fungal fimbriae. I. Structure, origin, and synthesis.

    abstract::Fine hair-like appendages on the cell walls of the another smut Ustilago violacea are described. These hairs are termed fimbriae because of their close similarity to the fimbriae (pili) found on certain Gram-negative bacteria. Cells of U. violacea may carry more than 200 fimbriae varying in length from about 0.5 mum t...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-076

    authors: Poon NH,Day AW

    更新日期:1975-04-01 00:00:00

  • Impact of liquid hog manure applications on antibiotic resistance genes concentration in soil and drainage water in field crops.

    abstract::Agricultural practices such as manure applications could contribute to the spread of antibiotic resistance genes (ARGs) within the environment. Our objective was to assess the impact of certain fertilization methods (mineral or manure) and tillage practices (reduced or conventional) on the presence of ARGs and bacteri...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2019-0343

    authors: Larouche É,Généreux M,Tremblay MÈ,Rhouma M,Gasser MO,Quessy S,Côté C

    更新日期:2020-10-01 00:00:00

  • Chemical and enzymatic changes in the cell walls of Candida albicans and Saccharomyces cerevisiae by scanning electron microscopy.

    abstract::Candida albicans and Saccharomyces cerevisiae cells were examined by scanning scanning electron microscopy before and after extraction of the mannans of the cell wall. The surfaces of control cells were smooth; after mannan extraction they were rough and showed erosions which were particularly striking within the area...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-164

    authors: Koch Y,Rademacher KH

    更新日期:1980-08-01 00:00:00

  • Characterization of bacteriophages with a lytic effect on various Salmonella serotypes and Escherichia coli O157:H7.

    abstract::Four phages isolated from cattle and poultry feces were analyzed for their ability to lyse Salmonella serotypes and Escherichia coli O157:H7. The phage one-step growth curves, morphology, and genetic characteristics were determined. All phages showed a lytic effect on various Salmonella serotypes and E. coli O157:H7, ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w11-099

    authors: López-Cuevas O,Castro-Del Campo N,León-Félix J,González-Robles A,Chaidez C

    更新日期:2011-12-01 00:00:00

  • Protective immune responses in mice induced by intramuscular and intranasal immunization with a Mycoplasma pneumoniae P1C DNA vaccine.

    abstract::Mycoplasma pneumoniae is an important causative agent of atypical pneumonia. This study was to determine the ability of a DNA expression vector, which encodes the carboxy terminal region of the M. pneumoniae P1 protein (P1C), to induce humoral and cellular immune responses and to protect against M. pneumoniae infecti...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-041

    authors: Zhu C,Wu Y,Chen S,Yu M,Zeng Y,You X,Xiao J,Wang S

    更新日期:2012-05-01 00:00:00

  • The effect of temperature and other growth conditions on the fatty acid composition of Escherichia coli.

    abstract::During exponential growth, strain AW405 of Escherichia coli K-12 did not regulate the fatty acid composition of its lipids in response either to temperature or to the addition of NaCl, KCl, or MgCl2 to the medium. Growth was severely restricted at temperatures below 21 degrees C. Differential scanning calorimetry (DSC...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m81-128

    authors: McGarrity JT,Armstrong JB

    更新日期:1981-08-01 00:00:00

  • Cyanide degradation by an Escherichia coli strain.

    abstract::Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-070

    authors: Figueira MM,Ciminelli VS,de Andrade MC,Linardi VR

    更新日期:1996-05-01 00:00:00

  • Internalization of Mycobacterium shottsii and Mycobacterium pseudoshottsii by Acanthamoeba polyphaga.

    abstract::Amoebae serve as environmental hosts to a variety of mycobacteria, including Mycobacterium avium and Mycobacterium marinum. Mycobacterium shottsii and Mycobacterium pseudoshottsii are waterborne species isolated from the spleens and dermal lesions of striped bass (Morone saxatilis) from the Chesapeake Bay. The optimal...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2013-0079

    authors: Gupta T,Fine-Coulson K,Karls R,Gauthier D,Quinn F

    更新日期:2013-08-01 00:00:00

  • Antibacterial activity of BCG-induced, interferon-containing sera.

    abstract::Sera from mice which have been vaccinated with BCG and challenged with old tuberculin contain gamma interferon. These same sera also express antibacterial activity. Using Staphylococcus aureus we demonstrated that its growth was inhibited at dilutions of sera as high as 1:320. A 4% concentration of sera reduced the gr...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m86-084

    authors: Moulton RG,Gerner GD

    更新日期:1986-05-01 00:00:00

  • Relationship between carbon source and susceptibility of Cephalosporium acremonium to selected amino acid analogues.

    abstract::The susceptibility of Cephalosporium acremonium to selected amino acid analogues was markedly influenced by the carbon source used in the test media. Lysine hydroxamate, beta-hydroxy norvaline, and hexafluorovaline were toxic when tested with ribose, ribose or fructose, and ribose or galactose, respectively. In contra...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m79-120

    authors: Mehta RJ,Nash CH

    更新日期:1979-07-01 00:00:00

  • Faecal bacterial composition in dairy cows shedding Mycobacterium avium subsp. paratuberculosis in faeces in comparison with nonshedding cows.

    abstract::The aim of this study was to determine possible differences in the faecal microbiota of dairy cows infected with Mycobacterium avium subsp. paratuberculosis (Johne's disease) in comparison with noninfected cows from the same herds. Faecal samples from cows in 4 herds were tested for M. avium subsp. paratuberculosis by...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2015-0814

    authors: Kaevska M,Videnska P,Sedlar K,Bartejsova I,Kralova A,Slana I

    更新日期:2016-06-01 00:00:00

  • Regulation and properties of an invertase from Clostridium pasteurianum.

    abstract::An intracellular invertase was induced in cultures of Clostridium pasteurianum utilizing sucrose as its carbon source for growth. This enzyme synthesis could be repressed by the addition of fructose of a sucrose-growing culture. In contrast, invertase activity was not affected by the addition of glucose to sucrose-gro...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-251

    authors: Laishley EJ

    更新日期:1975-11-01 00:00:00

  • Sensitivity of methanogenic bacteria to dicyclohexylcarbodiimide.

    abstract::Methanospirillum hungatei strains GP1 and JF1 when exposed to the adenosinetriphosphatase inhibitor N,N'-dicyclohexylcarbodiimide experienced a marked decline in growth rates, methane synthesis activities, and intracellular ATP concentrations. Although growth was inhibited, the intracellular ATP concentrations of all ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m82-146

    authors: Sprott GD,Jarrell KF

    更新日期:1982-08-01 00:00:00

  • Alterations in plant growth and in root hormone levels of lodgepole pines inoculated with rhizobacteria.

    abstract::The presence of other soil microorganisms might influence the ability of rhizobacterial inoculants to promote plant growth either by reducing contact between the inoculant and the plant root or by interfering with the mechanism(s) involved in rhizobacterially mediated growth promotion. We conducted the following exper...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w01-080

    authors: Bent E,Tuzun S,Chanway CP,Enebak S

    更新日期:2001-09-01 00:00:00

  • Effect of temperature on growth of guanidine-resistant mutants of poliovirus.

    abstract::Guanidine-resistant (gr) mutants of poliovirus were previously categorized into four groups by electrophoretic properties and peptide maps of nonstructural virus protein 2C. Growth of mutants in the presence of guanidine depends upon temperature of incubation. The four groups of gr variants respond differently to temp...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m85-220

    authors: Tershak DR

    更新日期:1985-12-01 00:00:00

  • Nucleotide sequence analysis of the gene encoding the Caulobacter crescentus paracrystalline surface layer protein.

    abstract::The entire nucleotide sequence of the rsaA gene, encoding the paracrystalline surface (S) layer protein (RsaA) of Caulobacter crescentus CB15A, was determined. The rsaA gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. Protease cleavage of mature RsaA protein and amino acid seque...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m92-033

    authors: Gilchrist A,Fisher JA,Smit J

    更新日期:1992-03-01 00:00:00

  • Cyst and germ tube wall structure in Aphanomyces astaci, Oomycetes.

    abstract::Aphanomyces astaci secondary cyst walls and walls of germinating spores were prepared by alkaline hydrolysis, autolysis, sonication, and enzymic degradation and were examined by shadow-casting and negative-staining techniques. The cyst wall consists of randomly oriented fibrils, about 3 nm in diameter. The fibrils are...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-210

    authors: Nyhlén L,Unestam T

    更新日期:1978-11-01 00:00:00

  • Effect of toluene on Pseudomonas stutzeri ST-9 morphology - plasmolysis, cell size, and formation of outer membrane vesicles.

    abstract::Isolated toluene-degrading Pseudomonas stutzeri ST-9 bacteria were grown in a minimal medium containing toluene (100 mg·L(-1)) (MMT) or glucose (MMG) as the sole carbon source, with specific growth rates of 0.019 h(-1) and 0.042 h(-1), respectively. Scanning (SEM) as well as transmission (TEM) electron microscope anal...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2016-0099

    authors: Michael E,Nitzan Y,Langzam Y,Luboshits G,Cahan R

    更新日期:2016-08-01 00:00:00

  • Complete genome sequence of Paracoccus denitrificans ATCC 19367 and its denitrification characteristics.

    abstract::Studies show that Paracoccus denitrificans can denitrify nitrogen sources under aerobic conditions. However, the lack of data on its genome sequence has restricted molecular studies and practical applications. In this study, the complete genome of P. denitrificans ATCC 19367 was sequenced and its nitrogen metabolism p...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2019-0037

    authors: Si YY,Xu KH,Yu XY,Wang MF,Chen XH

    更新日期:2019-07-01 00:00:00

  • Inactivation of human adenovirus by sequential disinfection with an alternative ultraviolet technology and monochloramine.

    abstract::In an effort to reduce human exposure to adenoviruses through drinking water, we determined the effectiveness of sequential disinfection with an alternative ultraviolet (UV) technology (medium-pressure (MP) UV) and monochloramine. The results of this study showed that MP UV was much more effective than traditional UV ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w10-047

    authors: Shin GA,Lee JK

    更新日期:2010-07-01 00:00:00

  • [Effect of monensin on the growth and secretion of exopolysaccharides in Botrytis cinerea Pers. and Sclerotium rolfsii Sacc].

    abstract::The addition of various concentrations of monensin (1,5, and 10 micrograms/mL) to the culture medium inhibits the fungal growth and perturbs exopolysaccharides secretion, provoking a decrease of production in Botrytis cinerea and an increase in Sclerotium rolfsii. The ionophore induces also modifications in both polym...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-124

    authors: Koulali Y,Talouizte A,Fonvieille JL,Dargent R

    更新日期:1996-09-01 00:00:00

  • The lipopolysaccharide (R type) as a common antigen of Neisseria gonorrhoeae. I. Immunizing properties.

    abstract::The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoea colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 microgram of LPS, and challenged intra...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-022

    authors: Diena BB,Ashton FE,Ryan A,Wallace R

    更新日期:1978-02-01 00:00:00