Abstract：
:Growth rates and cell yields of Mycobacterium convolutum strain R22 grown on a wide range of both odd- and even-numbered carbon solid n-alkanes decreased as the substrate carbon number increased. Cellular lipid was 2.5 times higher following growth on the hydrocarbon substrates. The amount of polar lipid was found to be about half of the cellular lipid in docosane through octacosane-grown cells. Phosphatidylethanolamine represented about 50% of the polar phospholipid in hydrocarbon- and nonhydrocarbon-grown cells. Phosphatidylserine, diphosphatidylglycerol, and phosphatidic acid were also found. The amount of phosphatidylserine was higher (10-14%) in docosane through octacosane-grown cells. There was a correspondingly smaller amount of diphosphatidylglycerol. These lipid changes may be associated with the assimilation of the hydrophobic substrates. The failure to detect label in lipids of solid n-alkane-grown cells incubated in the presence of [14C]acetate suggested that de novo synthesis of fatty acids did not occur to an appreciable extent. Transport of [14C]acetate and incorporation into cellular protein was not inhibited. Polar lipid fatty acid analyses indicated there was no direct incorporation of the oxidized substrate. The results suggest that beta-oxidation and a subterminal oxidative cleavage appear to be the major catabolic routes providing fatty acids, which are then incorporated into lipid.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Hallas LE,Vestal JRdoi
10.1139/m78-194subject
Has Abstractpub_date
1978-10-01 00:00:00pages
1197-203issue
10eissn
0008-4166issn
1480-3275journal_volume
24pub_type
杂志文章abstract：:Several probes for measuring dissolved carbon dioxide (CO2) concentration were installed in a 68-litre fermentor and their effectiveness compared. Submerged silastic rubber tubing gave reproducible results over a wide range of operating conditions and was generally superior to all other probes evaluated. The silastic ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-007
更新日期：1976-01-01 00:00:00
abstract：:An intracellular invertase was induced in cultures of Clostridium pasteurianum utilizing sucrose as its carbon source for growth. This enzyme synthesis could be repressed by the addition of fructose of a sucrose-growing culture. In contrast, invertase activity was not affected by the addition of glucose to sucrose-gro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-251
更新日期：1975-11-01 00:00:00
abstract：:Our freshwater caulobacter collection contains about 40 strains that are morphologically similar to Caulobacter crescentus. All elaborate a crystalline protein surface (S) layer made up of protein monomers 100-193 kDa in size. We conducted a comparative study of S-layer secretion in 6 strains representing 3 size group...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-046
更新日期：2004-09-01 00:00:00
abstract：:HI plasmids are distinguished by their thermosensitive mode of conjugation (transfer efficiency is optimal at 22-30 degrees C) and their capacity to encode multiple antibiotic resistance. These traits have implicated HI plasmids as potential vectors in the dissemination of antibiotic resistance among pathogenic and in...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-084
更新日期：1993-06-01 00:00:00
abstract：:A commercially available single-bottle blood culture system was evaluated at Ben Taub General Hospital, a Harris County District Hospital. Blood cultures from 1010 patients were examined with the Lederle Diagnostics one-bottle blood culture medium-SPS, Columbia broth (E-Vac, Pfizer), and an in-house-prepared brain hea...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-155
更新日期：1977-08-01 00:00:00
abstract：:Recent food-borne outbreaks of human listeriosis as well as numerous sporadic cases have been mainly caused by Listeria monocytogenes serovar 4b strains. Thus, it was of interest to find out whether a certain clone or a certain few clones were responsible for these cases and especially for outbreaks. We used pulsed-fi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-058
更新日期：1993-04-01 00:00:00
abstract：:The multi-drug resistant pathogen Acinetobacter baumannii displays unusual control of its SOS mutagenesis genes, as it does not encode a LexA repressor, but instead employs the UmuDAb repressor and a small DdrR protein that is uniquely found in Acinetobacter species. We used bacterial adenylate cyclase t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2020-0219
更新日期：2020-11-12 00:00:00
abstract：:Strain-specific fluorescent antibodies were prepared for three isolates of Aereomonas hydrophila. These antisera were reacted with 255 other A. hydrophila isolates, and their immunofluorescence was measured quantitatively by photometric fluorescence microscopy. Only 27.5% of the isolates reacted with the prepared anti...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-024
更新日期：1980-02-01 00:00:00
abstract：:Eighteen gram-negative marine bacteria and two terrestrial species, Escherichia coli and Pseudomonas aeruginosa, were examined for their sensitivity to lysis in distilled water after exposure to a salt solution containing a sea water concentration of Mg2+ (0.05 M) or to 0.5 M NaCl. A spectrum of lytic susceptibility w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-063
更新日期：1982-04-01 00:00:00
abstract：:Thirty Haemophilus strains and six Actinobacillus strains, all of porcine origin, were examined for their biochemical reactivity on API 20E and API ZYM test strips using dense cell suspensions (supplemented with NAD as appropriate) as strip inocula. When combined with a test for V-factor dependency, the use of both st...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-195
更新日期：1984-10-01 00:00:00
abstract：:The poly(3-hydroxybutyrate) (PHB) depolymerase structural gene of Comamonas sp. (phaZCsp) was cloned in Escherichia coli and identified by halo formation on PHB-containing solid medium. The nucleotide sequence of a 1719 base pair MboI fragment was determined and contained one large open reading frame (ORF1, 1542 base ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-183
更新日期：1995-01-01 00:00:00
abstract：:Influenza undergoes extensive antigenic variation in nature. These new antigenic variants invariably supplant the preceding antigenic type that then disappears. In apparent contrast to this, two H3N2 strains that were forwarded to the Laboratory Centre for Disease Control, Ottawa, in 1984 from a Canadian public health...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-139
更新日期：1987-09-01 00:00:00
abstract：:Bacillus stearothermophilus NCA 2184 lost viability and subsequently released cytoplasmic components when suspended in 0.1 M tris(hydroxymethyl)aminomethane (Tris) buffer (pH 7.2) and incubated at 60 degrees C. Cell lysis was prevented by the addition of 10 mM CaCl2 to the Tris-buffer suspension. Cells which were incu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-073
更新日期：1976-04-01 00:00:00
abstract：:Colletotrichum gloeosporioides is the causal agent of rubber tree anthracnose and leads to serious losses of natural rubber production. The pathogenesis of C. gloeosporioides on rubber tree remains unknown. Cerato-platanin proteins are small, secreted cysteine-rich proteins that contribute to virulence and function in...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0087
更新日期：2018-11-01 00:00:00
abstract：:The aim of this study was to determine the effect of the rhizosphere of maize on the diversity of denitrifying bacteria. Community structure comparison was performed by constructing a collection of isolates recovered from bulk and maize planted soil. A total of 3240 nitrate-reducing isolates were obtained and 188 of t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-037
更新日期：2004-07-01 00:00:00
abstract：:DNA hybridization studies were conducted to determine the taxonomic status of the aquatic group of Metschnikowia species and their varieties. Among the DNAs of the four varieties of Metschnikowia bicuspidata, that of Metschnikowia bicuspidata var. australis showed 37 to 51% relative binding with the DNAs of Metschniko...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-170
更新日期：1985-10-01 00:00:00
abstract：:Evidence has shown that individuals exposed to indoor toxic molds for extended periods of time have elevated risk of developing numerous respiratory illnesses. It is not clear at the cellular level what impact mold exposure has on the immune system. Herein, we show that 2 fungal volatiles (E)-2-octenal and oct-1-en-3-...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0708
更新日期：2014-01-01 00:00:00
abstract：:Antagonistic activity of the bacterium Pseudomonas cepacia against Trichoderma viride was greatly influenced by nutritional and environmental conditions. Xylose and trehalose strongly enhanced the antifungal activity of P. cepacia, whereas mannitol and glucose had little effect. The carbon sources that enhanced the an...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-152
更新日期：1991-11-01 00:00:00
abstract：:This research validates a novel approach for source tracking based on denaturing gradient gel electrophoresis (DGGE) analysis of DNA extracted from Escherichia coli isolates. Escherichia coli from different animal sources and from river samples upstream from, at, and downstream of a combined sewer overflow were subjec...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-083
更新日期：2007-10-01 00:00:00
abstract：:The activities of isocitrate lyase, esterase, and lipase by the psychrotrophic Acinetobacter sp. strain HH1-1 were monitored during incubation at 25 degreesC, 5 degreesC, and after a 25 degreesC to 5 degreesC down shift in growth temperature. During growth at 25 degreesC, isocitrate lyase activity was detected in cell...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:Considering the medical, biotechnological, and economical importance of actinobacteria, there is a continuous need to improve the tools for genetic engineering of a broad range of these microorganisms. Intergeneric conjugation has proven to be a valuable yet imperfect tool for this purpose. The natural resistance of m...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0041
更新日期：2015-08-01 00:00:00
abstract：:The use of two pulsed lasers as excitation sources in the immunofluorescent technique FTA-ABS (fluorescent treponemal antibody absorption) shows that excitation of specimens by continuous or long-duration pulses of light (in the order of a millisecond) degrades the fluorescence in such a way that it is impossible to q...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1975-08-01 00:00:00
abstract：:One of the proposed mechanisms by which rhizobacteria enhance plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin riboside (DHZR) in pure culture. Cytokinin production by Pseudomonas fluorescens G20-18, a rifa...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-029
更新日期：2001-05-01 00:00:00
abstract：:Deoxyribonuclease (DNase) activity was found in the culture fluids of numerous marine bacteria isolated from seawater. Among these ogranisms, marine bacterium, Vibrio sp., strain No. 2, showed the highest deoxyribonucleic acid-hydrolyzing activity. This organism requires salts of seawater for both growth and extracell...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-213
更新日期：1976-10-01 00:00:00
abstract：:Experiments were carried out to elucidate linkage between methane consumption and mineralization of P from different phosphorous sources. The treatments were no CH4 no P amendment absolute control, with CH4 no P amendment control, with CH4 + inorganic P as Ca3(PO4)2 and with CH4 + organic P (sodium phytate). P sources...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2020-0254
更新日期：2020-12-10 00:00:00
abstract：:The oomycetes, a distinct phylogenetic lineage of fungus-like microorganisms, are heterokonts (stramenopiles) belonging to the supergroup Chromalveolata. Although the complete genomic sequences of a number of oomycetes have been reported, little information regarding the introns therein is available. Here, we investig...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-103
更新日期：2011-02-01 00:00:00
abstract：:Bottles of medium were inoculated with both strains of Chromatiacea and Chlorobiaceae (mixed cultures) and immersed at differnt depths in the marine coastal waters of the Marseilles basin (France). After development, the in vivo absorption characteristics of the cells show the effect of the daylight penetration on the...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:Candida albicans and Saccharomyces cerevisiae cells were examined by scanning scanning electron microscopy before and after extraction of the mannans of the cell wall. The surfaces of control cells were smooth; after mannan extraction they were rough and showed erosions which were particularly striking within the area...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-164
更新日期：1980-08-01 00:00:00
abstract：:Aluminum (Al) toxicity is a major problem affecting soil fertility, microbial diversity, and nutrient uptake of plants. Rhizobia response and legume interaction under Al conditions are still unknown; it is important to understand how to develop and improve legume cultivation under Al stress. In this study, rhizobia re...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0019
更新日期：2018-08-01 00:00:00
abstract：:Cultured alveolar and peritoneal macrophages obtained from normal and immunized rabbits were used to induce wall-defective microbial variants of Pseudomonas pseudomallei. Variants were iduced only by normal alveolar macrophages. The variants reverted to typical Pseudomonas forms either spontaneously or upon transfer i...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-303
更新日期：1975-12-01 00:00:00
