Abstract:
:Highly distinctive aspects of the exponentail-phase Rhizobium japonicum cell were disclosed by means of thin sections, freeze etching, fluorescent antibodies, and ruthenium red staining. Polarity was expressed in the form of reserve polymer distribution near one end of the cell and as cytoplasmic localization near the opposite end. In addition, exocellular polysaccharide (EPS) accumulated preferentially around the cytoplasmic end, and the feature described previously as an "immunofluorescent polar tip" was seen clearly as an extracellular polar body (EPB) on the tip of the cell at the reserve polymer end. Compartmentalization of cytoplasm and reserves were consistent features of nearly all exponential cells of the two strains studied; strain 31, however, formed little EPS and had a high incidence of a large, tightly bound EPB, while strain 138 formed EPS extensively and had a low incidence of EPB. Extracellular polysaccharides of strain 138 reacted with soybean lectin in gel diffusion tests, so that the EPS seen in electron micrographs is tentatively considered to include the lectin-binding material. Extracellular polar bodies were accumulations of granular and fibrillar material with properties consistent with the presence of polysaccharide and lipopolysaccharide. The role of EPB in cell to cell attachment was confirmed by electron microscopy.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Tsien HC,Schmidt ELdoi
10.1139/m77-191subject
Has Abstractpub_date
1977-09-01 00:00:00pages
1274-84issue
9eissn
0008-4166issn
1480-3275journal_volume
23pub_type
杂志文章abstract::Fusaproliferin (FP), a toxic metabolite of the world-wide maize pathogens Fusarium proliferatum and Fusarium subglutinans, was recently found to be a natural contaminant of maize. Its toxic activity on haematopoietic human cell lines and its teratogenic effects on chicken embryos has been recently proved. Therefore a ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Human astrovirus (HAstV) was an important cause of viral gastroenteritis in infants in Wuhan city based on our previous study. The aim of the study was to investigate the nature of HAstV infection in Wuhan, People's Republic of China, especially in adults. Stool specimens were collected from 361 children and 301 adult...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-085
更新日期:2011-11-01 00:00:00
abstract::A 3.1-kb region of the bacteriophage D3 genome which contains the immunity functions has recently been sequenced (GenBank accession No. L22692). Sequence analysis indicated the presence of a putative repressor gene (c1) whose protein product functions to maintain the bacteriophage genome as a stably integrated prophag...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-030
更新日期:1997-03-01 00:00:00
abstract::The purpose of this study was to examine antimicrobial activity of endophytic fungi isolated from the leaves, stems, and roots of 54 species of Orchidaceae collected in a Brazilian tropical ecosystem. In total, 382 filamentous fungi and 13 yeast isolates were obtained and cultured to examine the production of crude ex...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W09-101
更新日期:2009-12-01 00:00:00
abstract::Using a well tested antibody specific for 3-hydroxy oxylipins, we mapped the presence of these oxylipins in selected Cryptococcus (Filobasidiella) species. Immunofluorescence microscopy studies revealed that these compounds are deposited on cell wall surfaces, appendages, and collarettes. In vitro studies revealed tha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w07-116
更新日期:2008-02-01 00:00:00
abstract::Enterococci, formerly confounded with faecal streptococci, are recognized since the beginning of the century as being faecal in origin and are generally searched for in waste waters and food products; their detection may in fact indicate the presence of enteropathogenic organisms. Although nearly ubiquitous, their pre...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract::The survival of and interactions between a Pseudomonas putida strain labelled with a red fluorescent protein gene (WT-rfp) and its green fluorescent protein gene-labelled rpoS(-) mutant (KO-gfp) were examined. The generation times of the planktonic WT-rfp and KO-gfp in trypticase soy broth were not significantly diffe...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-081
更新日期:2009-10-01 00:00:00
abstract::Lymphocytic choriomeningitis virus could be demonstrated earlier in mice inoculated with clinical specimens if the mice were sacrificed before they appeared sick for examination by the fluorescent antibody technique. In the procedure, the optimal route for inoculation was intracerebral and the best organ tested for st...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-245
更新日期:1976-11-01 00:00:00
abstract::Cellulomonas fimi genomic DNA was digested with HpaI, MunI, HindIII, and NsiI, producing fragments ranging in size from 20 to 1400 kbp that were resolved by pulsed field gel electrophoresis. Genetic and physical linkages were determined by Southern blotting and were used to construct a genome map. Cellulomonas fimi ha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::The immune benefits associated with the optimal intake of dietary fatty acids are widely known. The objective of the present investigation was to elucidate the role of omega-3 polyunsaturated fatty acids (n-3 PUFA) food source on acute pneumonia induced by Klebsiella pneumoniae. Three different n-3 PUFA preparations (...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0521
更新日期:2013-07-01 00:00:00
abstract::Neisseria gonorrhoeae strains can be grouped or differentiated (auxotyped) by their requirements for none, or any one or more of proline, uracil, hypoxanthine, and citrulline or ornithine. Most strains were readily assessed because they responded with growth or no growth on each defined auxanographic medium. Other str...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-204
更新日期:1983-10-01 00:00:00
abstract::A soluble cytochrome P450 (P450EP1A) induced by 2-ethoxyphenol was purified to apparent homogeneity from Corynebacterium sp. strain EP1. The P450EP1A showed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight of about 45 kDa. The CO-reduced difference spectra of P450EP1A ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::A species of Arthrobacter was recovered during culture of the causal organism of pitch canker of southern pines. Fusarium moniliforme var. subglutinans (FMS). Arthrobacter is a relatively common soil bacterium and is lytic to several fungal pathogens in the soil. Soil samples from two seed orchards with pitch canker a...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-004
更新日期:1981-01-01 00:00:00
abstract::The new United States Drinking Water Regulations state that water systems must analyze for Escherichia coli or fecal coliforms on any routine or repeat sample that is positive for total coliforms. The proposed methods for the detection of E. coli are based on beta-glucuronidase activity, using the fluorogenic substrat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-157
更新日期:1991-12-01 00:00:00
abstract::Passive protective activities of three different classes of monoclonal antibodies in mice against challenge with strain ATCC 31432 (capsular type I) of Staphylococcus epidermidis were examined. Monoclonal IgM antibody passively protected mice against challenge with the homologous strain, whereas monoclonal IgG1 and Ig...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-065
更新日期:1991-05-01 00:00:00
abstract::The repair of DNA damage caused by ultraviolet radiation (UVR) is well understood in both lower and higher organisms. Genetic studies carried out at optimum temperature for growth, 37 °C in Escherichia coli, have revealed the major pathways of DNA repair. We show that E. coli cells grown at 20 °C are more sensitive to...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0874
更新日期:2014-05-01 00:00:00
abstract::Symbiotic nitrogen fixation may be limited by the transport of C4 dicarboxylates into bacteroids in the nodule for use as a carbon and energy source. In an attempt to increase dicarboxylate transport, a plasmid was constructed in which the Rhizobium meliloti structural transport gene dctA was fused to a tryptophan ope...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-091
更新日期:1992-06-01 00:00:00
abstract::Corynebacterium glutamicum, a gram-positive bacterium, has been widely used for industrial amino acid production. Corynebacterium glutamicum his genes are located and transcribed in two unlinked loci, hisEG and hisDCB-orf1-orf2-hisHA-impA-hisFI. The latter his operon starts the transcription at the C residue localized...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-115
更新日期:2010-02-01 00:00:00
abstract::The deoxyribonucleic acid (DNA) of 36 isolates of Caryophanon latum and 3 isolates designated Caryophanon tenue, obtained from geographically diverse regions, has been examined for guanine plus cytosine (mol % G+C) base composition, genome size, and for relative reassociation levels. The isolates were found to be homo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-195
更新日期:1976-09-01 00:00:00
abstract::A series of synthetic promoters, based upon the Escherichia coli sigma 70 consensus promoter sequence, was constructed upstream of the lacZ reporter gene in the modified broad-host-range vector pQF52. The role of the intervening spacer region in gene expression in Pseudomonas aeruginosa and E. coli was studied by inse...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-141
更新日期:1997-10-01 00:00:00
abstract::The initial cause of the cessation of patulin biosynthesis in submerged cultures of Penicillium urticae was sought. The extrinsic limitations to patulin biosynthesis in fermentor cultures were successfully removed and kinetic profiles of the metabolites of the pathway and of three pathway enzymes were determined over ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-032
更新日期:1981-02-01 00:00:00
abstract::Two strains of Bacillus sphaericus. SSII-1 and 1593, were bioassayed for toxic activity against second-instar larvae of the mosquito Culex pipiens quinquefasciatus. It was found that strain 1593 developed a level of toxicity 3000 times that of strain SSII-1. Although the toxic activity of B. sphaericus SSII-1 was rela...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-193
更新日期:1979-11-01 00:00:00
abstract::There are inherent weaknesses associated with currently used bacterial fecal pollution indicator systems. Fecal pollution indicator data would be more meaningful if supplemented with information relating to the occurrence of pathogens in recreational water. Through surveys of four bathing beaches on Lake Ontario, it w...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-159
更新日期:1979-09-01 00:00:00
abstract::Eight Enterococcus faecium strains isolated from ewe milk and artisanal cheese from northwest Argentina were screened for biotechnological properties relevant to flavour development. The API ZYM test showed absence of proteases, presence of high amounts of peptidases, and high esterase-lipase activities. Low extracell...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-136
更新日期:2006-03-01 00:00:00
abstract::To facilitate the recognition of plant viruses with similar characteristics a dendrogram of characterized viruses was constructed. The sequence of criteria included: type of nucleic acid; single or double stranded; presence or absence of lipid envelope; helical or nonhelical symmetry; and divided or single genome. Non...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-197
更新日期:1977-09-01 00:00:00
abstract::Large numbers of minute, flat, long, straight, but often broken structures were observed in aqueous extracts of soil. We have applied the purely descriptive term 'tubules' to these structure, because they apparently became flattened during preparation for electron-microscopic viewing. The tubules appeared to be compos...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-105
更新日期:1979-06-01 00:00:00
abstract::Like recF and recQ mutants studied earlier, two other classes of Escherichia coli mutants defective in the RecF conjugal recombination pathway, recJ and recO, were found to be partially resistant to thymineless death. In contrast, a recN mutant, also belonging to the pathway, was indistinguishable from the wild type w...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-157
更新日期:1988-07-01 00:00:00
abstract::We report some characteristics of a ruminal bacterium (strain NPOH1) that metabolizes 3-nitropropanol, the toxic principle of various milk vetchs that are distributed worldwide. The gram-positive bacterium was nonmotile and did not produce spores. Growth of strain NPOH1 occurred under anaerobic conditions and was supp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-088
更新日期:1997-07-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with a range of infections, including various lower respiratory infections, otitis media, and conjunctivitis. There is some debate as to whether or not NTHi produces biofilms and, if so, whether or not this is relevant to pathogenesis. A...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0763
更新日期:2015-03-01 00:00:00
abstract::Urinary tract infections are common. Few published studies have demonstrated the change in Escherichia coli urinary isolate antimicrobial susceptibility over time within a given area and (or) population. The purpose of this study was to evaluate the change in susceptibility of E. coli clinical isolates obtained from u...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-001
更新日期:2012-03-01 00:00:00