Abstract：
:The survival of and interactions between a Pseudomonas putida strain labelled with a red fluorescent protein gene (WT-rfp) and its green fluorescent protein gene-labelled rpoS(-) mutant (KO-gfp) were examined. The generation times of the planktonic WT-rfp and KO-gfp in trypticase soy broth were not significantly different (i.e., p > 0.05) from each other at 30 degrees C. However, the biovolume of the KO-gfp biofilm was about 7 times larger than its WT-rfp counterpart after 48 h of growth. Furthermore, the presence of WT-rfp suppressed the biofilm development of KO-gfp significantly in co-culture biofilms. In planktonic conditions, the pre-carbon-starved WT-rfp achieved a 3-fold greater survival than the pre-carbon-starved KO-gfp in 0.85% saline after a 13-day incubation. In a 1:1 ratio co-culture, the pre-carbon-starved WT-rfp outcompeted the pre-carbon-starved KO-gfp by 20-fold. However, the survival of WT-rfp and KO-gfp were not significantly different from each other in biofilm conditions. Additionally, 11.4% and 61.2% of the WT-rfp and KO-gfp biofilms, respectively, remained intact after washing in 0.2% SDS for 60 min. In conclusion, the rpoS had a significant impact on survival and competitiveness of planktonic P. putida, and on biofilm development, being implicated in competitive suppression of biofilm development in co-culture biofilms and decreased biofilm cohesiveness.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Maki ML,Lawrence JR,Swerhone GD,Leung KTdoi
10.1139/w09-081subject
Has Abstractpub_date
2009-10-01 00:00:00pages
1176-86issue
10eissn
0008-4166issn
1480-3275pii
w09-081journal_volume
55pub_type
杂志文章abstract：:A high molecular component of a saline extract derived from Listeria monocytogenes contained amino acids, carbohydrates, and phosphorus. The same fraction was capable of promoting both the in vitro mitogenic and adjuvant activities and the in vivo immunosuppressive activity displayed by the crude extract. The material...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-204
更新日期：1982-12-01 00:00:00
abstract：:Subtyping Neisseria meningitidis by methods that rely on monoclonal antibody (mAb) reactivity results in an unusually high number of strains that are not subtypeable. To subtype 48 strains isolated (1993-1994) in the province of Quebec that were not subtypeable by mAb-based techniques, we used DNA sequencing of the va...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Cell-free extracts prepared from the extremely halophilic bacterium Halobacterium saccharovorum oxidize galactose and accumulate a product which reacts as if it were a lactone. The product does not act as a reducing sugar and contains all six of the carbon atoms initially present in galactose. The product was jugged t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-174
更新日期：1976-08-01 00:00:00
abstract：:To determine the seroprevalence of Norwalk virus and whether Norwalk virus contributed to an observed increase in illness in tap water drinkers participating in a prospective epidemiological study, sera collected during the study were examined for changes in Norwalk virus antibody titer, using a specific enzyme immuno...
journal_title：Canadian journal of microbiology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1139/m94-128
更新日期：1994-10-01 00:00:00
abstract：:5-Methoxymethyl-2'-deoxyuridine (MMUdR), a drug with potent antiviral activity in vitro against Herpes simplex virus, was investigated for its immunosuppressive effects. Doses as high as 2000mg/kg given daily for 9 days were not immunosupporessive as judged by the fact that treated animals produced normal immune respo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-158
更新日期：1977-08-01 00:00:00
abstract：:The aim of this study was to assess the influence of sodium content on the microbiota on the surface of ready-to-eat (RTE) meat products purchased from the retail market in Canada. Products, including sliced and sausage-type deli meats, were analysed with culture-dependent and culture-independent methods. Bacteria wer...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0630
更新日期：2015-02-01 00:00:00
abstract：:In Japan, antimicrobial agent therapy for patients with diarrhea due to enterovirulent organisms including enterohemorrhagic Escherichia coli (EHEC) is common, and norfloxacin (NFLX), fosfomycin, and kanamycin are recommended for EHEC treatment by the Japanese Ministry of Health and Welfare. The aim of this study was ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：:Mixtures of anionic HgCl3-/HgCl4(2)-complexes were less toxic to terrestrial bacteria (Erwinia herbicola, Agrobacterium tumefaciens), to marine bacteria (Acinetobacter sp., Aeromonas sp.), and to bacteriophages (phi 11 M 15 of Staphylococcus aureus and P1 of Escherichia coli) than were equivalent concentrations of Hg ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-197
更新日期：1979-11-01 00:00:00
abstract：:Fluorescent Pseudomonas spp. isolated from fruiting bodies (FB) of Cantharellus cibarius were characterized physiologically and genetically and were compared with fluorescent Pseudomonas from forest soil and with sequences from the GenBank database. Pseudomonas spp. from FB differed physiologically from isolates from ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-062
更新日期：2002-08-01 00:00:00
abstract：:Pathogenic strains of Vibrio cholerae O139 possess the cholera toxin A subunit (ctxA) gene as well as the gene for toxin co-regulated pili (tcpA). We report the isolation of a ctxA-negative, tcpA-negative V. cholerae O139 strain (INDREI) from a patient in Mexico diagnosed with gastrointestinal illness. Certain phenoty...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-004
更新日期：2003-01-01 00:00:00
abstract：:Virulent strains of the fish pathogen Aeromonas salmonicida, which have surface S-layers (S+), efficiently adhere to, enter, and survive within macrophages. Here we report that S+ bacteria were 10- to 20-fold more adherent to non-phagocytic fish cell lines than S-layer-negative (S-) mutants. When reconstituted with ex...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-034
更新日期：2000-07-01 00:00:00
abstract：:Effects of temperature and anaerobic conditions on the replication of two bacteriophages, nt-1 and nt-6, of the estuarine bacterium Vibrio natriegens were studied. Reduction in temperature resulted in longer latent periods and reduced burst sizes for both phages. Replication under anaerobic conditions resulted in long...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-053
更新日期：1978-03-01 00:00:00
abstract：:Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-038
更新日期：2004-08-01 00:00:00
abstract：:Fourteen Lactococcus lactis strains showing inhibitory activity against Listeria innocua SICC 4202 were isolated from different French raw milks and raw milk cheeses and screened for bacteriocin production by the triple layer method under conditions that eliminate the effects of lactic acid and hydrogen peroxide. Thre...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-114
更新日期：1995-09-01 00:00:00
abstract：:Virus-like particles have been found in specific regions of the reproductive tracts of three different braconid wasps, all parasitoids of the tobacco budworm, Heliothis virescens. The particles are nuclear in origin, and Feulgen cytochemistry of particulate fluid in the calyx and oviducts of one species has revealed t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-148
更新日期：1976-07-01 00:00:00
abstract：:Bacterial communities that can degrade surfactants have been selected from coastal seawaters contaminated by urban sewages. Only the linear fraction of commercial anionic surfactants was quickly degraded, and a residual fraction representing 10% of the initial concentration always remained. The highest concentrations ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：:In Salmonella typhimurium LT2 the F factor of Escherichia coli K-12 replicates normally but is repressed; Flac+ cells give no visible lysis on solid media with male-specific phages, low frequency transfer of Flac+ (0.001-0.007 per donor cell), few f2 infective centers (0.002-0.006 per cell), and they propagate male-sp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-184
更新日期：1983-09-01 00:00:00
abstract：:Fermentative hydrogen production was carried out by Enterobacter cloacae DM11, using glucose as the substrate. The effects of initial substrate concentration, initial medium pH, and temperature were investigated. Results showed that at an initial glucose concentration of 1.0% (m/v), the molar yield of hydrogen was 3.3...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-005
更新日期：2006-06-01 00:00:00
abstract：:Two-dimensional (2-D) polyacrylamide gel electrophoresis was used to detect proteins induced in Frankia sp. strain ACN14a-tsr by root exudates of its symbiotic host, Alnus glutinosa. The 5 most prominent proteins were purified from 2-D gels and characterized by N-terminal sequencing. All of these proteins had a high p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-046
更新日期：2001-06-01 00:00:00
abstract：:Bacteria associated with 6 habitats of groundnut were evaluated for their broad-spectrum antifungal activity and suppression of collar rot (Aspergillus niger) of groundnut. Three hundred and ninety-three strains were tested against 8 fungal pathogens of groundnut including 5 necrotrophic fungi, Aspergillus flavus, A. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-119
更新日期：2005-02-01 00:00:00
abstract：:The accumulation of radioactive Cs by fungi was studied by analysis of fruit bodies (n (total) = 205, n greater than or equal to 5 for 22 species) collected in 1988 in a Norwegian mountain area with high deposition of radiocaesium from the Chernobyl accident. To account for site variation, the radiocaesium content of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-119
更新日期：1990-10-01 00:00:00
abstract：:Subunits A and B of cholera enterotoxin were isolated by chromatography on a Bio-Gel P-60 column in the presence of 4% formic acid. The purity and biological activity of the isolated subunits was assessed by polyacrylamide disc gel electrophoresis and mouse adrenal cell assay, respectively. The specific uptake of isol...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-153
更新日期：1978-08-01 00:00:00
abstract：:A real-time polymerase chain reaction assay that uses degenerate primers and a dual-labelled probe was developed to detect the bexA gene of Haemophilus influenzae, including those belonging to non-b serotypes as well as clonal division II strains. This assay is sensitive and specific, detecting 20 copies of the gene, ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0040
更新日期：2013-05-01 00:00:00
abstract：:Group B streptococcus type III is a major cause of neonatal death. The terminal sialic acid moiety of the group B streptococcus type specific capsule has been shown to be an important virulence factor. We demonstrate here that bacteria grown in human serum have increased cell surface sialic acid content compared with ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-016
更新日期：1994-02-01 00:00:00
abstract：:A bacterial strain capable of utilizing 3,6-dimethylphenanthrene (3,6-DMP) as its sole source of carbon and energy was isolated from a creosote-contaminated soil. The isolate was identified as a strain of Sphingomonas sp. and was designated strain JS1. Utilization of 3,6-DMP was demonstrated by an increase in bacteria...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-018
更新日期：2003-02-01 00:00:00
abstract：:The environmental distribution of Giardia spp. and Cryptosporidium spp. is dependent upon human, agricultural, and wildlife sources. The significance of each source with regard to the presence of parasites in the environment is unknown. This 2-year study examined parasite prevalence in human sewage influent, wildlife,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-047
更新日期：2002-06-01 00:00:00
abstract：:Caulobacters are biofilm-forming members of the natural flora of soil and aquatic environments, which exhibit several characteristics that make them attractive for development of high surface area microbial bioreactors or biosensors. Although caulobacters are well characterized genetically, little is known about their...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:An investigation of brucellosis caused by Brucella suis, type 4, in reindeer, Rangifer tarandus L., and other ungulates inoculated experimentally with virulent isolates was undertaken to observe the course of infection, follow titres of serum agglutins, and determine the extent to which intraspecific and interspecific...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-024
更新日期：1978-02-01 00:00:00
abstract：:Endophytic microorganisms reside asymptomatically within plants and are a source of new bioactive products for use in medicine, agriculture, and industry. Colletotrichum (teleomorph Glomerella) is a fungus widely cited in the literature as a producer of antimicrobial substances. Identification at the species level, ho...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-059
更新日期：2009-09-01 00:00:00
abstract：:Bacterial biofilms are commonly formed on medical devices and food processing surfaces. The antimicrobials used have limited efficacy against the biofilms; therefore, new strategies to prevent and remove these structures are needed. Here, the effectiveness of brief oxidative treatments, based on the combination of sod...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0747
更新日期：2015-05-01 00:00:00
