Abstract：
:Subtyping Neisseria meningitidis by methods that rely on monoclonal antibody (mAb) reactivity results in an unusually high number of strains that are not subtypeable. To subtype 48 strains isolated (1993-1994) in the province of Quebec that were not subtypeable by mAb-based techniques, we used DNA sequencing of the variable regions of porA, a gene that encodes the class 1 outer membrane protein. We assigned subtypes to all the previously nonserosubtypeable isolates and identified some novel subtypes. Because our sequencing strategy included the promoter region of porA, different isolates were compared in their sequences of the porA promoter region. A poly(G) stretch lies between the -10 and -35 regions of the promoter; replacement of a G residue by an A residue in this region resulted in loss of expression of porA. No correlation was found between the number of G residues in the poly(G) stretch and the level of expression; a minimum of 10 G residues is required in this stretch for expression of porA. One isolate expressed no class 1 outer membrane protein because of the insertion sequence IS1301 in the coding region of porA. Another isolate did not express the protein owing to a frame-shift mutation within the coding region of porA. Sequencing of porA allowed assignments of subtypes to previously uncharacterized isolates and provided insights about the regulation of expression of this gene in N. meningitidis.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Arhin FF,Moreau F,Coulton JW,Mills ELsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
56-63issue
1eissn
0008-4166issn
1480-3275journal_volume
44pub_type
杂志文章abstract：:We modified a technique, cDNA-AFLP, for identifying differentially expressed genes in plants to work in the filamentous fungus Leptosphaeria maculans (Desmaz.) Ces. & De Not. The cDNA fragments generated by our method ranged in size from approximately 100 to 400 bps. On average, twice as many cDNA fragments were ampli...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-100
更新日期：2001-10-01 00:00:00
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-032
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-147
更新日期：1981-09-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-039
更新日期：1980-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-058
更新日期：1995-04-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-093
更新日期：1978-05-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-032
更新日期：1976-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-092
更新日期：2003-11-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-162
更新日期：1997-12-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-010
更新日期：1990-01-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-141
更新日期：1976-07-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-030
更新日期：2009-07-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/w2012-025
更新日期：2012-05-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-134
更新日期：1977-07-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0703
更新日期：2016-07-01 00:00:00
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journal_title：Canadian journal of microbiology
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doi：10.1139/cjm-2014-0399
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journal_title：Canadian journal of microbiology
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更新日期：2005-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-101
更新日期：1992-07-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-021
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/m95-118
更新日期：1995-10-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-114
更新日期：1985-07-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-231
更新日期：1975-10-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2020-0239
更新日期：2021-01-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0019
更新日期：2018-08-01 00:00:00
abstract：:A method to obtain synchronous sporulation during idiophase development in liquid surface cultures of Rhizopus arrhizus Fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. In this system growth and sporulation are effectively separated. The respiratory pattern indicat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-151
更新日期：1977-08-01 00:00:00
