Abstract:
:In this study, 16S rRNA gene primers were designed to complement the suite of already available PCR primers for the detection of different methanogens involved in biogas production through anaerobic digestion by SYBR Green real-time PCR. Primers designed for use in TaqMan real-time PCR for the organisms Methanosaeta, Methanosarcina, and Methanoculleus have been described previously; however, we found that (i) the Methanoculleus primers were not specific to members of the genus and that (ii) the Methanosarcina primers did not work specifically with SYBR Green real-time PCR. Thus, we designed new primers for these and other methanogens, and we optimized SYBR Green real-time PCR assays. Primers were tested by end-point and real-time PCR, and they were found to work specifically and sensitively. Application of these primers will allow the detection and quantification of Methanoculleus, Methanosarcina, Methanothermobacter, and a group of yet uncultured archaea from anaerobic habitats.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Franke-Whittle IH,Goberna M,Insam Hdoi
10.1139/w08-157subject
Has Abstractpub_date
2009-05-01 00:00:00pages
611-6issue
5eissn
0008-4166issn
1480-3275pii
w08-157journal_volume
55pub_type
杂志文章abstract::Vaccination is the most promising strategy to reduce the incidence of pneumococcal infection. Although there are vaccines available, all of them are based on polysaccharide antigens (conjugated or not). In addition to their high cost, those vaccines do not cover all serotypes. To overcome these hindrances, we evaluate...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-083
更新日期:2012-09-01 00:00:00
abstract::A dairy product processing plant was studied for 2.5 years to examine contamination with Staphylococcus aureus and try to correlate the source of contamination. Cultures were submitted to an antibiotic susceptibility test (AST) and characterised by Pulsed-field Gel Electrophoresis (PFGE) analysis. Results showed that ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-111
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abstract::Three strains, Rhodotorula rubra, Rhodotorula glutinis, and Candida zeylanoides, isolated from fish, were tested for the expression of putative tissue colonization factors. All strains were able to bind collagen type I, fibronectin, and laminin to various degrees after growing on various solid and broth media, while t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-171
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-228
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-043
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-141
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-259
更新日期:1975-11-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-141
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journal_title:Canadian journal of microbiology
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doi:10.1139/W07-052
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-151
更新日期:1979-09-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-076
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abstract::Twenty-six strains of group JK corynebacteria had the same colonial morphology and biological reactions as the biotypes of the biovars of Corynebacterium genitalium and C. pseudogenitalium. Therefore, group JK corynebacteria can be assigned to the biovars of C. genitalium or C. pseudogenitalium. Although the strains d...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-164
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journal_title:Canadian journal of microbiology
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doi:10.1139/m94-016
更新日期:1994-02-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-036
更新日期:2003-04-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-024
更新日期:2005-06-01 00:00:00
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2002-08-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:1979-09-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-127
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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