Functional analysis of sigma-70 consensus promoters in Pseudomonas aeruginosa and Escherichia coli.

abstract：:Photorhabdus temperata, an insect pathogen and nematode symbiont, is motile in liquid medium by swimming. We found that P. temperata was capable of surface movement, termed swarming behavior. Several lines of evidence indicate that P. temperata use the same flagella for both swimming and swarming motility. Both motili...

journal_title：Canadian journal of microbiology

authors： Michaels B,Tisa LS

更新日期：2011-03-01 00:00:00

abstract：:Catalase from Bacillus sp. N2a (BNC) isolated from Antarctic seawater was purified to homogeneity. BNC has a molecular mass of about 230 kDa and is composed of four identical subunits of 56 kDa. The catalase showed optimal activity at 25 degrees C and at a pH range of 6-11. The enzyme could be inhibited by azide, hydr...

journal_title：Canadian journal of microbiology

authors： Wang W,Sun M,Liu W,Zhang B

更新日期：2008-10-01 00:00:00

abstract：:Our purpose was to study bacterial antagonism between a limited number of strictly anaerobic strains and Clostridium perfringens in the intestinal tract of gnotobiotic rodents. Gnotobiotic mice harboring a Bacteroides thetaiotaomicron, a Fusobacterium necrogenes, and a Clostridium sp. strain were protected against pat...

journal_title：Canadian journal of microbiology

authors： Yurdusev N,Nicolas JL,Ladire M,Ducluzeau R,Raibaud P

更新日期：1987-03-01 00:00:00

abstract：:Of 24 bacterial species examined for lisinopril refractive peptidyl dipeptidase activity, only 8 contained activity. Activity in Pseudomonas maltophilia was more than fourfold higher than that of any other species. Pseudomonas maltophilia may be unique among bacteria in possessing high peptidyl dipeptidase activity th...

journal_title：Canadian journal of microbiology

authors： Stevens J,Fanburg BL,Lanzillo JJ

更新日期：1990-01-01 00:00:00

abstract：:Recent food-borne outbreaks of human listeriosis as well as numerous sporadic cases have been mainly caused by Listeria monocytogenes serovar 4b strains. Thus, it was of interest to find out whether a certain clone or a certain few clones were responsible for these cases and especially for outbreaks. We used pulsed-fi...

journal_title：Canadian journal of microbiology

authors： Buchrieser C,Brosch R,Catimel B,Rocourt J

更新日期：1993-04-01 00:00:00

abstract：:Eighteen gram-negative marine bacteria and two terrestrial species, Escherichia coli and Pseudomonas aeruginosa, were examined for their sensitivity to lysis in distilled water after exposure to a salt solution containing a sea water concentration of Mg2+ (0.05 M) or to 0.5 M NaCl. A spectrum of lytic susceptibility w...

journal_title：Canadian journal of microbiology

authors： Laddaga RA,MacLeod RA

更新日期：1982-04-01 00:00:00

abstract：:A polygalacturonase was isolated from the culture medium of Sclerotinia borealis, a psychrophilic fungus that grows on lawn and wheat seedling under the snow in winter and induces the snow mold disease. Pectic acid was a better substrate of this enzyme than pectin when the activity was determined by measuring the redu...

journal_title：Canadian journal of microbiology

authors： Takasawa T,Sagisaka K,Yagi K,Uchiyama K,Aoki A,Takaoka K,Yamamato K

更新日期：1997-05-01 00:00:00

abstract：:Dark spots disease (DSD) is a relatively new coral disease that has become one of the most prevalent afflictions in the Caribbean Sea. To partially characterize bacterial communities associated with DSD, carbon utilization patterns of bacterial strains isolated from the surface mucopolysaccharide layers of healthy and...

journal_title：Canadian journal of microbiology

authors： Gil-Agudelo DL,Fonseca DP,Weil E,Garzón-Ferreira J,Smith GW

更新日期：2007-04-01 00:00:00

abstract：:Nodulation of soybeans by indigenous and inoculum strains of Bradyrhizobium japonicum was studied in field experiments in Wisconsin from 1983 to 86. Aqueous suspensions of bacteria were applied to seeds at the time of planting at levels of 7 × 10(7)-10(10) bacteria per 2.5-cm row. The predominant indigenous serogroup ...

journal_title：Canadian journal of microbiology

authors： McLoughlin TJ,Alt SG,Merlo PA

更新日期：1990-11-01 00:00:00

abstract：:The open reading frame pp0053, which has a high homology with the sequence of mitochondrial sulfide dehydrogenase (HMT2) conferring cadmium tolerance in fission yeast, was amplified from Pseudomonas putida KT2440 and expressed in Escherichia coli JM109(DE3). The isolated and purified PP0053-His showed absorption spect...

journal_title：Canadian journal of microbiology

authors： Shibata H,Kobayashi S

更新日期：2006-08-01 00:00:00

abstract：:The N5,N10-methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum strain Marburg has been purified with reasonable yield and much higher specific activity than previously reported. For the first time it has been shown that both N5,N10-methylenetetrahydromethanopterin dehydrogenase and...

journal_title：Canadian journal of microbiology

authors： Mukhopadhyay B,Daniels L

更新日期：1989-04-01 00:00:00

abstract：:Chloroquine increases the inhibition of cultured lymphocytes by high concentrations of phytohemagglutinin (PHA) or concanavalin A (Con A). The inhibition is also increased by complement. Thus chloroquine and complement have similar effects. Time-course studies show that chloroquine increases the rate of onset of the c...

journal_title：Canadian journal of microbiology

authors： Forsdyke DR

更新日期：1975-10-01 00:00:00

abstract：:An active in vitro protein-synthesizing system has been developed from Rhodospirillum rubrum grown under either photosynthetic or heterotrophic conditions. A protease activity has been found in both of these systems, and this activity can be readily inactivated by treating the cells with KCl and phenylmethyl sulfonylf...

journal_title：Canadian journal of microbiology

authors： Chow CT

更新日期：1976-02-01 00:00:00

abstract：:We modified a technique, cDNA-AFLP, for identifying differentially expressed genes in plants to work in the filamentous fungus Leptosphaeria maculans (Desmaz.) Ces. & De Not. The cDNA fragments generated by our method ranged in size from approximately 100 to 400 bps. On average, twice as many cDNA fragments were ampli...

journal_title：Canadian journal of microbiology

authors： Gellatly KS,Ash GJ,Taylor JL

更新日期：2001-10-01 00:00:00

abstract：:Humicola lutea 103 is a copper-tolerant fungal strain able to grow in the presence of 300 μg·mL(-1) Cu(2+) under submerged cultivation. To prevent the consequences of copper overload, microorganisms have evolved molecular mechanisms that regulate its uptake, intracellular traffic, storage, and efflux. In spite of this...

journal_title：Canadian journal of microbiology

authors： Krumova ETs,Stoitsova SR,Paunova-Krasteva TS,Pashova SB,Angelova MB

更新日期：2012-12-01 00:00:00

abstract：:Glutaraldehyde, the biological fixative of choice in the cytochemical localization of the phosphatases, was investigated for its effects on Pseudomonas aeruginosa alkaline phosphatase. Comparative studies on the inactivation of alkaline phosphatase by glutaraldehyde showed significant differences when the purified pro...

journal_title：Canadian journal of microbiology

authors： Day DF,Ingram JM

更新日期：1981-05-01 00:00:00

abstract：:The induction of nitrogenase (C2H2) activity in asymbiotically cultured Rhizobium sp. 32H1 was found to be associated with morphological changes in the cells which were more pronounced than those seen in bacteroids. Polyphosphate granules were found in both bacteroids and cultured cells, but poly-beta-hydroxybutyrate ...

journal_title：Canadian journal of microbiology

authors： van Brussel AA,Costerton JW,Child JJ

更新日期：1979-03-01 00:00:00

abstract：:The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical...

journal_title：Canadian journal of microbiology

authors： Van Eck WH

更新日期：1976-11-01 00:00:00

abstract：:Monoclonal antibodies reacting with the B subunit of Vibrio cholerae O1 strain 569B cholera toxin (CT-B) were used to identify unique and common epitopes of V. cholerae non-O1 and Vibrio mimicus CT-B. Vibrio cholerae non-O1 strains produced CT-B showing three monoclonal antibody reaction patterns (epitypes), which cor...

journal_title：Canadian journal of microbiology

authors： Tamplin ML,Jalali R,Ahmed MK,Colwell RR

更新日期：1990-06-01 00:00:00

abstract：:Vero cells were more suitable then BHK-21 cells for the detection of rubella-specific IgG antibodies because the nonspecific fluorescence was minimal. However, BHK-21 cells were found more sensitive than Vero cells for the detection of rubella-specific IgM antibodies. ...

journal_title：Canadian journal of microbiology

authors： Payment P,Roy L,Gilker JC,Chagnon A

更新日期：1975-03-01 00:00:00

abstract：:Diversity of actinomycetes and their antifungal activities against some plant pathogenic fungi were examined in various vegetative soils from 14 different sites in the western part of Korea. Actinomycete counts ranged from 1.17 x 10(6) to 4.20 x 10(6) cfu x g(-1) dried soil. A total of 1510 actinomycetes were isolated...

journal_title：Canadian journal of microbiology

authors： Lee JY,Hwang BK

更新日期：2002-05-01 00:00:00

abstract：:Neisseria gonorrhoeae strains can be grouped or differentiated (auxotyped) by their requirements for none, or any one or more of proline, uracil, hypoxanthine, and citrulline or ornithine. Most strains were readily assessed because they responded with growth or no growth on each defined auxanographic medium. Other str...

journal_title：Canadian journal of microbiology

authors： Hendry AT

更新日期：1983-10-01 00:00:00

abstract：:The effects of the Clostridium difficile toxin were examined in HeLa and mouse adrenal tumor (MAT) cells. Cytotoxicity was evaluated by vital dye exclusion and 51Cr release. In both HeLa and MAT cells, C. difficile toxin caused rounding of virtually 100% of cells. This rounding was distinguishable from rounding produc...

journal_title：Canadian journal of microbiology

authors： Gurwith MJ,Langston C,Dunsmore B

更新日期：1982-01-01 00:00:00

abstract：:To develop a library-dependent method of tracking fecal sources of contamination of beaches on the Atlantic coast of southwestern France, a library of 6368 Escherichia coli isolates was constructed from samples of feces, from 40 known human or animal sources collected in the vicinity of Arcachon Bay in 2010, and in Fr...

journal_title：Canadian journal of microbiology

authors： Garabetian F,Vitte I,Sabourin A,Moussard H,Jouanillou A,Mornet L,Lesne M,Lyautey E

更新日期：2020-12-01 00:00:00

abstract：:Polymerase chain reaction amplification was used to compare different regions of the cytochrome c3 gene from nine strains of Desulfovibrio vulgaris, to examine homology within the species. Six 30-base polymerase chain reaction primers and three probes were synthesized on the basis of the published nucleic acid sequenc...

journal_title：Canadian journal of microbiology

authors： Kwoh DY,Vedvick TS,McCue AF,Gevertz D

更新日期：1993-04-01 00:00:00

abstract：:A rapid and efficient electroporation procedure has been developed for transformation of germinating conidia of filamentous fungi. Pretreatment of conidial preparations with a cell wall weakening agent, such as beta-glucuronidase, was found to be essential for successful transformation. Using the qa-2+ gene of Neurosp...

journal_title：Canadian journal of microbiology

authors： Chakraborty BN,Patterson NA,Kapoor M

更新日期：1991-11-01 00:00:00

abstract：:For the recently described serotype 15 of biotype I and serotypes 13 and 14 of biotype II of Actinobacillus pleuropneumoniae, fhuA and hgbA were detected by polymerase chain reaction and DNA sequencing. To determine the substrate specificity of the iron receptors FhuA and HgbA and to study their role in the virulence ...

journal_title：Canadian journal of microbiology

authors： Shakarji L,Mikael LG,Srikumar R,Kobisch M,Coulton JW,Jacques M

更新日期：2006-04-01 00:00:00

abstract：:No clear data are available on how flavonoids from different chemical groups affect root colonization by arbuscular mycorrhizal fungi (AMF) and whether flavonoids affecting the presymbiotic growth of AMF also affect root colonization by AMF. In the present work, we compared the effect of flavones (chrysin and luteolin...

journal_title：Canadian journal of microbiology

authors： Scervino JM,Ponce MA,Erra-Bassells R,Bompadre J,Vierheilig H,Ocampo JA,Godeas A

更新日期：2007-06-01 00:00:00

abstract：:In an effort to reduce human exposure to adenoviruses through drinking water, we determined the effectiveness of sequential disinfection with an alternative ultraviolet (UV) technology (medium-pressure (MP) UV) and monochloramine. The results of this study showed that MP UV was much more effective than traditional UV ...

journal_title：Canadian journal of microbiology

authors： Shin GA,Lee JK

更新日期：2010-07-01 00:00:00

abstract：:Marine shrimp of the species Penaeus monodon were collected from the coastal region (Haroa) of the deltaic Sundarbans of West Bengal, India during the premonsoon period in 1996. Klebsiella pneumoniae was isolated from the alimentary canal and gills of the shrimp as the sole isolate. All 10 isolated strains were resist...

journal_title：Canadian journal of microbiology

authors： Choudhury P,Kumar R

更新日期：1998-02-01 00:00:00