Abstract：
:Polymerase chain reaction amplification was used to compare different regions of the cytochrome c3 gene from nine strains of Desulfovibrio vulgaris, to examine homology within the species. Six 30-base polymerase chain reaction primers and three probes were synthesized on the basis of the published nucleic acid sequence of the cytochrome c3 gene from D. vulgaris, NCIMB 8303. Amplifications were performed on genomic DNA isolated from NCIMB 8303 as well as eight other strains. Six strains, NCIMB 8302, 8305, 8306, 8311, 11779, and DSM 2119, showed amplification products of equal size and quantity to those of strain 8303. Two other strains, NCIMB 8456 and DSM 1744, either showed reduced levels or no detectable amplification products. These results were confirmed by hybridization of amplified DNA to radiolabeled probes specific for each product. DNA sequencing of a 145-bp polymerase chain reaction fragment from strains NCIMB 8302, 8303, 11779, and DSM 2119 revealed complete sequence homology between these strains, whereas slight differences were seen with strain NCIMB 8456. Amino acid sequencing of the first 20 residues of cytochrome c3 purified from strains NCIMB 8456 and 8303 also showed differences in the two proteins. In contrast to the results obtained with strain NCIMB 8456, limited homology was observed between the first 20 amino acid residues of cytochrome c3 from strain DSM 1744 and strain NCIMB 8303.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Kwoh DY,Vedvick TS,McCue AF,Gevertz Ddoi
10.1139/m93-059subject
Has Abstractpub_date
1993-04-01 00:00:00pages
402-11issue
4eissn
0008-4166issn
1480-3275journal_volume
39pub_type
杂志文章abstract：:We report some characteristics of a ruminal bacterium (strain NPOH1) that metabolizes 3-nitropropanol, the toxic principle of various milk vetchs that are distributed worldwide. The gram-positive bacterium was nonmotile and did not produce spores. Growth of strain NPOH1 occurred under anaerobic conditions and was supp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-088
更新日期：1997-07-01 00:00:00
abstract：:The presence of killer and proteolytic yeasts was studied among 944 isolates representing 105 species from tropical yeast communities. We found 13 killer toxin producing species, with Pichia kluyveri being the most frequent. Other killer yeast isolates were Candida apis, Candida bombicola, Candida fructus, Candida kru...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-046
更新日期：1997-04-01 00:00:00
abstract：:Mucor alternans(van Tieghem) was found to be a diphasic species in that it grew exclusively in the yeast-like budding phase under anaerobic conditions in the complex medium yeast extract-peptone-glucose broth and in tissue-culture medium 199. In the latter medium this growth form occurred also at 37C, at an initial pH...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-010
更新日期：1975-01-01 00:00:00
abstract：:The correlation between the occurrence of indicator organisms and salmonellae in the same sample homogenate of various classes of dried foods was studied. In the first phase of the work involving 5837 samples, 1943 contained fecal coliforms and 100 contained Salmonella. Fecal coliforms were not detected in 53 of the S...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-141
更新日期：1976-07-01 00:00:00
abstract：:Giardia duodenalis is a common intestinal parasite in most parts of the world. In Canada it is associated with both endemic and epidemic infections that are often transmitted by the waterborne route. Although G. duodenalis strains have been isolated from several animals, the role of other mammals in human infection is...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-171
更新日期：1986-12-01 00:00:00
abstract：:To establish the relevance of the cell wall in metal ion transport, cobalt uptake was examined in Neurospora crassa. Cobalt taken up was largely surface bound (>90%), resulting in a release of calcium and magnesium. Surface-bound cobalt could not enter intracellular locations upon further incubation of mycelia in a me...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-096
更新日期：2005-12-01 00:00:00
abstract：:Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (DNA-DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C)) and relative genome sizes were determined for selected strains...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-083
更新日期：1979-05-01 00:00:00
abstract：:In British Columbia, Canada, drinking water is tested for total coliforms and Escherichia coli, but there is currently no routine follow-up testing to investigate fecal contamination sources in samples that test positive for indicator bacteria. Reliable microbial source tracking (MST) tools to rapidly test water sampl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0618
更新日期：2013-03-01 00:00:00
abstract：:Previously, we reported the metabolic responses of Pseudomonas sp. strain HF-1, a nicotine-degrading bacterium, to nicotine stress. However, the metabolic effects of nicotine on non-nicotine-degrading bacteria that dominate the environment are still unclear. Here, we have used nuclear magnetic resonance based metabolo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0206
更新日期：2014-08-01 00:00:00
abstract：:Monoclonal antibodies reacting with the B subunit of Vibrio cholerae O1 strain 569B cholera toxin (CT-B) were used to identify unique and common epitopes of V. cholerae non-O1 and Vibrio mimicus CT-B. Vibrio cholerae non-O1 strains produced CT-B showing three monoclonal antibody reaction patterns (epitypes), which cor...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-071
更新日期：1990-06-01 00:00:00
abstract：:To assess the effect of fire and salvage logging on the diversity of mycorrhizal-bacterial communities, bacteria associated with Cenococcum, Thelephora, Tomentella, Russulaceae, and E-strain ectomycorrhizae (ECM) of Abies lasiocarpa seedlings were characterized using two approaches. First, bacteria were isolated and c...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-056
更新日期：2002-07-01 00:00:00
abstract：:Several probes for measuring dissolved carbon dioxide (CO2) concentration were installed in a 68-litre fermentor and their effectiveness compared. Submerged silastic rubber tubing gave reproducible results over a wide range of operating conditions and was generally superior to all other probes evaluated. The silastic ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-007
更新日期：1976-01-01 00:00:00
abstract：:Epiphytic tracer bacteris (a Pseudomonas sp. and an Arthrobacter sp.) from soybean buds were introduced into open flowers of greenhouse-grown soybean plants. Of the 177 resulting pods cultured after surface disinfection, tracers were recovered from within 24. Seed from two of these pods also carried tracers. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-064
更新日期：1976-03-01 00:00:00
abstract：:The production of cold shock proteins (csps) and cold acclimation proteins (caps) was characterized in the psychrotrophic bacterium Pseudomonas putida Q5 and its transconjugant P. putida Q5T which contains the toluene-degradative TOL (pWWO) plasmid, using two-dimensional gel electrophoresis and computing scanning lase...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-100
更新日期：1996-08-01 00:00:00
abstract：:To screen, isolate, and characterize bacterial populations producing microcin J25, we report here two rapid, reliable, and sensitive methods, using polymerase chain reaction and colony blot hybridization with a digoxigenin-labelled probe. A sample of 26 Escherichia coli strains isolated from poultry intestinal content...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:The influence of plant growth promoting (PGP) activity of bacterial communities recovered from each of six weed species (barnyard grass (Echinochloa crusfalli (L.) Beauv.), corn spurrey (Spergula arvensis L.), goldenrod (Sonchus sp.), Italian ryegrass (Lolium multiflorum L.), lamb's-quarters (Chenopodium album L.), an...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-110
更新日期：2001-11-01 00:00:00
abstract：:Fifty-two killer, factor-resistant, nuclear mutants were isolated from sensitive strains of yeast and assorted into three functional groups. All but one mutant owed their resistance to an alteration in the cell wall binding site for killer. In several mutant strains, an alteration at the site of killer binding was ass...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-041
更新日期：1978-03-01 00:00:00
abstract：:Dietary exposure to heavy metals may have detrimental effects on human and animal health, even at low concentrations. Specific probiotic bacteria may have properties that enable them to bind toxins from food and water. We assessed the interaction of probiotic bacteria with cadmium and lead in vitro as an initial scree...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-043
更新日期：2006-09-01 00:00:00
abstract：:Conidia of Torula caligans (Batista & Upadhyay) M. B. Ellis comb.nov. and T. terrestris Misra were examined by transmission- and scanning-electron microscopy. Torula caligans produced four-celled conidia in which the central cells were distinctly larger than the basal and apical cells. Conidia of T. terrestris were 4-...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-279
更新日期：1975-12-01 00:00:00
abstract：:The transmission of multi-drug resistant pathogens and antimicrobial-resistant genes is an arising problem with multiple factors involved (humans, domestic animals, wildlife). The aim of this study was to investigate the presence of Escherichia coli isolates with different antimicrobial resistance genes from backyard ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2020-0280
更新日期：2021-01-04 00:00:00
abstract：:From the four known isoforms of the staphylococcal exfoliative toxins (ETs), only ETA and ETB are the major causative agents. General knowledge is that the gene for ETA is located on the chromosome, whereas that for ETB is located on a large plasmid. Yoshizawa and co-workers (2000, Microbiol. Immunol. 44(3): 189-191) ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0721
更新日期：2013-04-01 00:00:00
abstract：:Human astrovirus (HAstV) was an important cause of viral gastroenteritis in infants in Wuhan city based on our previous study. The aim of the study was to investigate the nature of HAstV infection in Wuhan, People's Republic of China, especially in adults. Stool specimens were collected from 361 children and 301 adult...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-085
更新日期：2011-11-01 00:00:00
abstract：:The site specificity of metallic ion binding in Escherichia coli K-12 lipopolysaccharide was assessed by collecting high-resolution phosphorus nuclear magnetic resonance spectra in the presence of manganese, a paramagnetic divalent cation. This technique revealed high-affinity interactions between the cation and all o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-010
更新日期：1986-01-01 00:00:00
abstract：:When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests incre...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W08-035
更新日期：2008-06-01 00:00:00
abstract：:Fungal chitinases play essential roles in chitin degradation, cell wall remodeling, chitin recycling, nutrition acquisition, autolysis, and virulence. In this study, 18 genes of the glycoside hydrolase 18 (GH18) family were identified in the Isaria cicadae genome. Seventeen of the genes belonged to chitinases and one ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2019-0129
更新日期：2020-04-01 00:00:00
abstract：:Degradation of purified rubella virus by heat treatment (37, 45, or 56 degrees C) revealed the following structures. The viral envelope, a modified cellular membrane, bears spherical subunits, 5-6 nm in diameter, hexamers, or pentamers. Two glycoproteins, VP-2 (50 000 daltons) and VP-3 (63 000 daltons), are associated...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1975-05-01 00:00:00
abstract：:We studied the persistence of naturally acquired cell-mediated immunity to rubella during early pregnancy. We compared lymphocyte transformation responses to phytohemagglutinin and rubella virus in 35 naturally immune women aged 17-37 years, in the first trimester of pregnancy, with 49 naturally immune age-matched non...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-257
更新日期：1983-12-01 00:00:00
abstract：:Eighteen yeast species of the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus, each one represented by its type strain, were investigated with the objective of evaluating their carotenoid composition. The pigments were extracted from yeast cells, quantified by high pressure liquid chromatography ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-068
更新日期：2007-08-01 00:00:00
abstract：:Seventeen of 22 group A meningococcal strains were separated into four serotypes by a microbactericidal assay using group A antisera. In addition, 6 of 19 of these strains were killed by a group B antiserum and similar cidal patterns were obtained with a group C antiserum. There was little correlation between type and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-139
更新日期：1979-08-01 00:00:00
abstract：:Two potassium (K)-bearing minerals, Nanjing feldspar and Suzhou illite, were used to investigate K mobilization by the wild-type strain NBT of Bacillus edaphicus, also labeled MPs+, selected for high activity in mobilizing potassium from minerals, and by four of its UV + LiCl mutants, MPs++, MPs+1, MPs+2, and MPs-. In...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-117
更新日期：2006-01-01 00:00:00
