Abstract:
:The effects of inhibition of protein synthesis on the cell size distributions of rel+ and relA- derivatives of Escherichia coli K-12 were determined. Amino acid deprivation resulted in a reduction in the cell sizes of rel+ strains but not of relA- strains. Treatment with chloramphenicol (CAM) did not alter the size distributions of either rel+ or relA- strains except when they were rel+ dap-. CAM treatment of rel+ dap- strains resulted in an increase in cell size. It is proposed that these results reflect differences in the structures of the cell envelopes of rel+ and relA- bacteria.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Ishiguro EE,Ramey WDdoi
10.1139/m78-127subject
Has Abstractpub_date
1978-06-01 00:00:00pages
761-4issue
6eissn
0008-4166issn
1480-3275journal_volume
24pub_type
杂志文章abstract::Citrate synthase (citrate-oxaloacetate lyase (CoA acetylating), EC 4.1.3.7) has been purified to electrophoretic homogeneity from a marine Pseudomonas. The enzyme was made up of identical subunits, with a molecular wieght of about 53 000, as determined by sodium dodecyl sulphate - polyacrylamide gel electrophoresis. T...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-039
更新日期:1978-03-01 00:00:00
abstract::The rapid onset of resistance reduces the efficacy of most conventional antimicrobial drugs and is a general cause of concern for human well-being. Thus, there is great demand for a continuous supply of novel antibiotics to combat this problem. Bacteria-derived antimicrobial peptides (AMPs) have long been used as food...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/cjm-2014-0613
更新日期:2015-02-01 00:00:00
abstract::Group B streptococcus type III is a major cause of neonatal death. The terminal sialic acid moiety of the group B streptococcus type specific capsule has been shown to be an important virulence factor. We demonstrate here that bacteria grown in human serum have increased cell surface sialic acid content compared with ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-016
更新日期:1994-02-01 00:00:00
abstract::A soluble cytochrome P450 (P450EP1A) induced by 2-ethoxyphenol was purified to apparent homogeneity from Corynebacterium sp. strain EP1. The P450EP1A showed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight of about 45 kDa. The CO-reduced difference spectra of P450EP1A ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::This study, the first of arbovirus activity in Prince Edward Island, has shown antibodies to the snowshoe hare strain of the California encephalitis group virus to be present in sera of wild and domestic animals; 15.35% of snowshoe hare, 20% of equine. and 2.5% of bovine sera were positive for hemagglutination inhibit...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-188
更新日期:1981-11-01 00:00:00
abstract::The 40-kDa porin protein of Haemophilus influenzae type b was reconstituted into proteoliposomes. The relative rates of diffusion of small uncharged sugars across the channels formed by this protein were determined by measuring the rates of osmotic swelling of the liposomes. From these rates, a pore diameter of 1.8 nm...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-027
更新日期:1988-02-01 00:00:00
abstract::We examined factors related to the potent antagonistic effect of Escherichia coli and Bacteroides ovatus on Staphylococcus aureus in anaerobic continuous flow cultures. In the presence of sugars fermentable by E. coli alone or both E. coli and S. aureus, motile E. coli strains exerted a potent antagonistic effect and ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-106
更新日期:1988-05-01 00:00:00
abstract::Candida albicans and Saccharomyces cerevisiae cells were examined by scanning scanning electron microscopy before and after extraction of the mannans of the cell wall. The surfaces of control cells were smooth; after mannan extraction they were rough and showed erosions which were particularly striking within the area...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-164
更新日期:1980-08-01 00:00:00
abstract::The adherence of Streptococcus pneumoniae to epithelial (A549) lung cells was studied and the bacterial binding distribution was found to be nonrandom (non-Gaussian). Analysis of the dependency of bacterial binding on the cell cycle of A549 cells revealed that approximately 1.8 times more bacteria bind to G2 cells tha...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::A disseminated and fatal infection was established in C57BL mice, injected intraperitoneally with either Neisseria meningitidis B,2b or Haemophilus influenzae type b bacteria plus enhancement factors. The effects of mucin, hemoglobin, and iron dextran as enhancement of bacterial infectivity in mice were evaluated indi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-007
更新日期:1986-01-01 00:00:00
abstract::Sera from mice which have been vaccinated with BCG and challenged with old tuberculin contain gamma interferon. These same sera also express antibacterial activity. Using Staphylococcus aureus we demonstrated that its growth was inhibited at dilutions of sera as high as 1:320. A 4% concentration of sera reduced the gr...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-084
更新日期:1986-05-01 00:00:00
abstract::Numbers of viable bacteria as indicated by colony-forming units (CFU), microbial adenosine triphosphate levels, dissolved organic carbon (DOC), particulate organic carbon and nitrogen (POC and PON), glucose concentration, heterotrophic potential (Vmax), and in situ heterotrophic activity (Vn) of near-surface microlaye...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-251
更新日期:1976-12-01 00:00:00
abstract::Overexpression and purification are procedures used to allow functional and structural characterization of proteins. Many overexpressed proteins are partially or completely insoluble, and can not be easily purified. The NifA protein is an enhancer-binding protein involved in activating the expression of nif and some f...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-094
更新日期:2000-11-01 00:00:00
abstract::Dithiothreitol reacted, at pH 9.0, with the isolated cell walls of Methanospirillum hungatii, to release about 23% of the cell wall dry weight as a high molecular weight fraction (> 0.5 million daltons). Untreated walls consisted of 70% amino acids, 11% lipid, and 6.6% carbohydrate. Sugars were identified as rhamnose,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-017
更新日期:1980-02-01 00:00:00
abstract::Influenza undergoes extensive antigenic variation in nature. These new antigenic variants invariably supplant the preceding antigenic type that then disappears. In apparent contrast to this, two H3N2 strains that were forwarded to the Laboratory Centre for Disease Control, Ottawa, in 1984 from a Canadian public health...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m87-139
更新日期:1987-09-01 00:00:00
abstract::A purified lipopolysaccharide (LPS) isolated from the rickettsial agent of Q fever, Coxiella burnetii phase 1, possesses some of the in vivo and in vitro biological properties previously only associated with bacterial endotoxins. The Coxiella LPS is mitogenic for guinea pig leukocytes, induces non-specific resistance ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-259
更新日期:1978-12-01 00:00:00
abstract::In Japan, antimicrobial agent therapy for patients with diarrhea due to enterovirulent organisms including enterohemorrhagic Escherichia coli (EHEC) is common, and norfloxacin (NFLX), fosfomycin, and kanamycin are recommended for EHEC treatment by the Japanese Ministry of Health and Welfare. The aim of this study was ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::Antibiotic susceptibility tests and restriction enzyme analysis (REA) of genomic DNA were performed to characterize the relationship between sources of isolates of yellow-pigmented enterococci. Antibiotic susceptibility tests were conducted with 10 therapeutic antibiotics and 54 isolates grouped by source (wild and ot...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-44-11-1066
更新日期:1998-11-01 00:00:00
abstract::During exponential growth, strain AW405 of Escherichia coli K-12 did not regulate the fatty acid composition of its lipids in response either to temperature or to the addition of NaCl, KCl, or MgCl2 to the medium. Growth was severely restricted at temperatures below 21 degrees C. Differential scanning calorimetry (DSC...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-128
更新日期:1981-08-01 00:00:00
abstract::The natural reservoirs and biological characteristics of pathogenic populations of both subspecies of Photobacterium damselae in aquatic habitants remain unclear because of difficulties in obtaining pathogenic strains from the environment. In the present study, we assessed the occurrence of Photobacterium damselae sub...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-021
更新日期:2011-05-01 00:00:00
abstract::The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1-2 d. In the buffer system, the 1- and...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-131
更新日期:2005-03-01 00:00:00
abstract::Transcriptional profiles of 2 unrelated clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates were analyzed following 10% (v/v) ethanol challenge (15 min), which arrested growth but did not reduce viability. Ethanol-induced stress (EIS) resulted in differential gene expression of 1091 genes, 600 common ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2017-0221
更新日期:2017-09-01 00:00:00
abstract::Concentric bodies were found in vegetative and pycnidial cells of Podoxythium tricothecium and in ascostromal paraphyses of Rhytidhysterium rufulum, two representatives of the Loculoascomycetes. They averages 185 nm in diameter in P. trichothecium and 255 nm in R. rufulum and consisted of three zones. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-218
更新日期:1977-10-01 00:00:00
abstract::Eighteen gram-negative marine bacteria and two terrestrial species, Escherichia coli and Pseudomonas aeruginosa, were examined for their sensitivity to lysis in distilled water after exposure to a salt solution containing a sea water concentration of Mg2+ (0.05 M) or to 0.5 M NaCl. A spectrum of lytic susceptibility w...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-063
更新日期:1982-04-01 00:00:00
abstract::The metabolism of cyclohexanecarboxylic acid by a bacterium, designated PRL W19, follows a pathway involving beta-oxidation of coenzyme A intermediates analogous to the classical oxidation of fatty acids. The organism appears to have the property for the constitutive metabolism of caproic acid, and cell extracts conta...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-141
更新日期:1978-07-01 00:00:00
abstract::Amoebae serve as environmental hosts to a variety of mycobacteria, including Mycobacterium avium and Mycobacterium marinum. Mycobacterium shottsii and Mycobacterium pseudoshottsii are waterborne species isolated from the spleens and dermal lesions of striped bass (Morone saxatilis) from the Chesapeake Bay. The optimal...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0079
更新日期:2013-08-01 00:00:00
abstract::A method for the production of conjugated linoleic acid (CLA) from linoleic acid (LA) using growing cultures of Propionibacterium freudenreichii ssp. shermanii JS was developed. The growth inhibitory effect of LA was eliminated by dispersing it in a sufficient concentration of polyoxyethylene sorbitan monooleate deter...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::Campylobacter fetus is a recognized pathogen of cattle and sheep that can also infect humans. No adhesins specific for C. fetus have to date been identified; however, bacterial attachment is essential to establish an infecting population. Scanning electron microscopy revealed C. fetus attachment to the serosal surface...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w07-115
更新日期:2008-01-01 00:00:00
abstract::Penicillium chrysogenum F1 is very efficient in bioleaching heavy metals from the soil and is used for that purpose. We found that F1 can extract 19.8 mg Cd, Cu, Pb, and Zn from 2.5 g soil; the total heavy metals' bioleaching ratio was 60.4%. In this study, the bioleaching mechanism was investigated by means of metabo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0624
更新日期:2019-09-01 00:00:00
abstract::Haemophilus paragallinarum secretes metalloproteases into different culture media lacking serum. Secreted proteins, concentrated by precipitation with 70% ammonium sulphate ((NH(4))(2)SO(4)) or methanol, displayed proteolytic activity at >100 kDa molecular mass in 10% polyacrylamide gels co-polymerized with porcine ge...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-067
更新日期:2005-10-01 00:00:00