Directed biosynthesis of novel derivatives of echinomycin. II. Purification and structure elucidation.

abstract：:A microplaque reduction assay for human and mouse interferons is described, using plastic disposable multicompartmental plates with flat-bottomed wells of 6 mm in diameter. The procedure is rapid and reproducible and lends itself to a considerable degree of automation. Its potential application in other areas of virol...

journal_title：Canadian journal of microbiology

authors： Campbell JB,Grunberger T,Kochman MA,White SL

更新日期：1975-08-01 00:00:00

abstract：:The yeast Saccharomyces cerevisiae Y222 was studied in the presence of the following iron-binding agents: Desferal, dipyridyl, and human and bovine transferrins. We report that cell growth and lanosterol 14 alpha-demethylase cytochrome P450 are not affected by Desferal but that dipyridyl and serum transferrins decreas...

journal_title：Canadian journal of microbiology

authors： Wright GD,Honek JF

更新日期：1989-10-01 00:00:00

abstract：:Citrate synthase (citrate-oxaloacetate lyase (CoA acetylating), EC 4.1.3.7) has been purified to electrophoretic homogeneity from a marine Pseudomonas. The enzyme was made up of identical subunits, with a molecular wieght of about 53 000, as determined by sodium dodecyl sulphate - polyacrylamide gel electrophoresis. T...

journal_title：Canadian journal of microbiology

authors： Higa AI,Massarini E,Cazzulo JJ

更新日期：1978-03-01 00:00:00

abstract：:The metal-binding properties of Pseudomonas aeruginosa PAO1 biofilms were investigated using four metals (Cu, Fe, Au, and La). All but one of the metals (i.e., Cu) were bound by the biofilms in amounts that were significantly greater than those bound by planktonically grown cells of the same strain. Lanthanum precipit...

journal_title：Canadian journal of microbiology

authors： Langley S,Beveridge TJ

更新日期：1999-07-01 00:00:00

abstract：:Mutants of Trichoderma harzianum with altered antibiotic production were isolated using ultraviolet light mutagenesis. These included strains whose activity in a Fusarium oxysporum spore germination assay was greater than twice that of the parental strain and one that had no detectable antifungal activity. Characteris...

journal_title：Canadian journal of microbiology

authors： Graeme-Cook KA,Faull JL

更新日期：1991-09-01 00:00:00

abstract：:Chitinases isolated from membrane and cytosolic fractions of two mucoraceous fungi, Choanephora cucurbitarum and Phascolomyces articulosus, were investigated. The membrane-bound chitinase was isolated by Bio-Gel P-100 and DEAE Bio-Gel A chromatographic techniques. On SDS-PAGE the chitinase from both fungi migrated as ...

journal_title：Canadian journal of microbiology

authors： Balasubramanian R,Manocha MS

更新日期：1992-04-01 00:00:00

abstract：:Bacterial community structure and the effects of several environmental factors on the microbial community distribution were investigated in the sediment of the eutrophic Lake Xuanwu. Profiles of bacterial communities were generated using denaturing gradient gel electrophoresis (DGGE), and the results were interpreted ...

journal_title：Canadian journal of microbiology

authors： Zeng J,Yang LY,Liang Y,Li JY,Xiao L,Jiang LJ,Zhao DY

更新日期：2008-12-01 00:00:00

abstract：:In the frame of a survey of potentially endophytic N2-fixing Burkholderia associated with maize in Mexico, its country of origin, the soil of an indigenous maize field near Oaxaca was studied. Under laboratory conditions, plant seedlings of two ancient maize varieties were used as a trap to select endophyte candidates...

journal_title：Canadian journal of microbiology

authors： Estrada P,Mavingui P,Cournoyer B,Fontaine F,Balandreau J,Caballero-Mellado J

更新日期：2002-04-01 00:00:00

abstract：:Pinus monticola antimicrobial peptide 1 (Pm-AMP1) was expressed and purified from bacterial cell lysate and its identity and purity confirmed by Western blot analysis using the Pm-AMP1 antibody. Application of Pm-AMP1 resulted in visible hyphal growth inhibition of Cronartium ribicola , Phellinus sulphurascens , O...

journal_title：Canadian journal of microbiology

authors： Zamany A,Liu JJ,Ekramoddoullah A,Sniezko R

更新日期：2011-08-01 00:00:00

abstract：:Seventeen of 22 group A meningococcal strains were separated into four serotypes by a microbactericidal assay using group A antisera. In addition, 6 of 19 of these strains were killed by a group B antiserum and similar cidal patterns were obtained with a group C antiserum. There was little correlation between type and...

journal_title：Canadian journal of microbiology

authors： Quan AL,Sippel JE

更新日期：1979-08-01 00:00:00

abstract：:A microcinematographic analysis of the behaviour and movements of cells and cell masses in mated cultures (NC4 X VI2) of Dictyostelium discoideum indicates that a chemotactic process directs cell aggregation during macrocyst development. Zygote giant cells form before aggregation begins and act as the aggregation cent...

journal_title：Canadian journal of microbiology

authors： O'Day DH

更新日期：1979-12-01 00:00:00

abstract：:The inhibition of pyruvate oxidative decarboxylation as a means of decreasing ruminal methanogenesis in vitro was studied. In the first experiment, the addition of adenosine and adenine (with and without ribose) to ruminal batch cultures did not decrease methanogenesis. In the second experiment, the addition of oxythi...

journal_title：Canadian journal of microbiology

authors： Ungerfeld EM,Rust SR,Burnett R

更新日期：2003-10-01 00:00:00

abstract：:Diversity of actinomycetes and their antifungal activities against some plant pathogenic fungi were examined in various vegetative soils from 14 different sites in the western part of Korea. Actinomycete counts ranged from 1.17 x 10(6) to 4.20 x 10(6) cfu x g(-1) dried soil. A total of 1510 actinomycetes were isolated...

journal_title：Canadian journal of microbiology

authors： Lee JY,Hwang BK

更新日期：2002-05-01 00:00:00

abstract：:The use of microbial fertilizers can help to avoid the harmful effects of traditional agricultural fertilizers and pesticides; however, there are many constraints on the practical application of such fertilizers. In this study, microbial biochar formulations (MBFs) were obtained by loading biochar, created from agricu...

journal_title：Canadian journal of microbiology

authors： Tao S,Wu Z,Wei M,Liu X,He Y,Ye BC

更新日期：2019-05-01 00:00:00

abstract：:Alkaline phosphatase (APase) was present in the bovine rumen in both cell-free and cell-associated states and levels of the enzyme varied with dietary regime. Reaction product deposition showed that the enzyme was associated with the mixed bacterial population. No enzyme was observed to be associated with protozoa. Tr...

journal_title：Canadian journal of microbiology

authors： Cheng KJ,Hironaka R,Costerton JW

更新日期：1976-05-01 00:00:00

abstract：:Haemophilus paragallinarum secretes metalloproteases into different culture media lacking serum. Secreted proteins, concentrated by precipitation with 70% ammonium sulphate ((NH(4))(2)SO(4)) or methanol, displayed proteolytic activity at >100 kDa molecular mass in 10% polyacrylamide gels co-polymerized with porcine ge...

journal_title：Canadian journal of microbiology

authors： Rivero-García PC,Cruz CV,Alonso PS,Vaca S,Negrete-Abascal E

更新日期：2005-10-01 00:00:00

abstract：:Southern bean mosaic virus (SBMV) protein was cleaved with cyanogen bromide and a highly basic peptide, CB-1, was isolated by ion exclusion and ion-exchange chromatography. Twelve peptides were separated from a tryptic digest of CB-1 by ion-exchange chromatography and the composition of these peptides was similar to t...

journal_title：Canadian journal of microbiology

authors： Tremaine JH,Ronald WP,Kelly EM

更新日期：1980-12-01 00:00:00

abstract：:Antagonistic activity of the bacterium Pseudomonas cepacia against Trichoderma viride was greatly influenced by nutritional and environmental conditions. Xylose and trehalose strongly enhanced the antifungal activity of P. cepacia, whereas mannitol and glucose had little effect. The carbon sources that enhanced the an...

journal_title：Canadian journal of microbiology

authors： Upadhyay RS,Visintin L,Jayaswal RK

更新日期：1991-11-01 00:00:00

abstract：:HI plasmids are distinguished by their thermosensitive mode of conjugation (transfer efficiency is optimal at 22-30 degrees C) and their capacity to encode multiple antibiotic resistance. These traits have implicated HI plasmids as potential vectors in the dissemination of antibiotic resistance among pathogenic and in...

journal_title：Canadian journal of microbiology

authors： Maher D,Taylor DE

更新日期：1993-06-01 00:00:00

abstract：:Symbiotic nitrogen fixation may be limited by the transport of C4 dicarboxylates into bacteroids in the nodule for use as a carbon and energy source. In an attempt to increase dicarboxylate transport, a plasmid was constructed in which the Rhizobium meliloti structural transport gene dctA was fused to a tryptophan ope...

journal_title：Canadian journal of microbiology

authors： Rastogi V,Labes M,Finan T,Watson R

更新日期：1992-06-01 00:00:00

abstract：:Pseudomonas aeruginosa ATCC 9027 grew on 0.5% (v/v) hexadecane as a sole carbon source in a chemically defined medium which required the addition of Fe3+ and Ca2+. There was a variable and extended lag period before an active growth rate was attained. Visible light microscopic evidence revealed that the bacteria did n...

journal_title：Canadian journal of microbiology

authors： Miguez CB,Beveridge TJ,Ingram JM

更新日期：1986-03-01 00:00:00

abstract：:The objective of the present work was to evaluate the relevance of the 2-methylcitric acid cycle (2MCC) to the catabolism of propionate in Burkholderia sacchari. Two B. sacchari mutants unable to grow on propionate were obtained: one disrupted in acnM, and the other in acnM and prpC deleted. An operative 2MCC signific...

journal_title：Canadian journal of microbiology

authors： Pereira EM,Silva-Queiroz SR,Cabrera Gomez JG,Silva LF

更新日期：2009-06-01 00:00:00

abstract：:In the dairy industry, bacterial contaminants persist on equipment surfaces due to spore and biofilm formation. These are involved in cross-contamination problems that affect the quality of processed products and limit their life. The pasteurization process, in which milk is submitted to moderate heat treatment, is in...

journal_title：Canadian journal of microbiology

authors： Malek F

更新日期：2019-06-01 00:00:00

abstract：:A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...

journal_title：Canadian journal of microbiology

authors： King EJ,Brown MF

更新日期：1983-06-01 00:00:00

abstract：:Epiphytic tracer bacteris (a Pseudomonas sp. and an Arthrobacter sp.) from soybean buds were introduced into open flowers of greenhouse-grown soybean plants. Of the 177 resulting pods cultured after surface disinfection, tracers were recovered from within 24. Seed from two of these pods also carried tracers. ...

journal_title：Canadian journal of microbiology

authors： Leben C

更新日期：1976-03-01 00:00:00

abstract：:The prevalence of Coxiella burnetti infection (Q fever) was determined among Nova Scotia (N.S.) and Prince Edward Island (P.E.I.) blood donors by using the complement fixation and microimmunofluorescence (IF) test. The complement fixation and IF antibody tests measured antibody prevalence for the phase II or phase I a...

journal_title：Canadian journal of microbiology

authors： Marrie TJ,Van Buren J,Faulkner RS,Haldane EV,Williams JC,Kwan C

更新日期：1984-01-01 00:00:00

abstract：:Through genomic analysis of mucosa-associated Escherichia coli strains, we found a close genetic association among isolates from pediatric inflammatory bowel disease (IBD) patients. A specific E. coli pathovar, adherent-invasive E. coli (AIEC), was found in Crohn's disease (CD) adult patients - this pathovar has enhan...

journal_title：Canadian journal of microbiology

authors： Schippa S,Iebba V,Totino V,Santangelo F,Lepanto M,Alessandri C,Nuti F,Viola F,Di Nardo G,Cucchiara S,Longhi C,Conte MP

更新日期：2012-04-01 00:00:00

abstract：:Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC 3.1.3.2), cyclic phosphodiesterase, ribonuclease I (EC 3.1.4.22), and phosphoglucose isomerase (EC 5.3.1.9) (PGI is at least par...

journal_title：Canadian journal of microbiology

authors： Chatterjee AK,Ross H,Sanderson KE

更新日期：1976-10-01 00:00:00

abstract：:The presence of other soil microorganisms might influence the ability of rhizobacterial inoculants to promote plant growth either by reducing contact between the inoculant and the plant root or by interfering with the mechanism(s) involved in rhizobacterially mediated growth promotion. We conducted the following exper...

journal_title：Canadian journal of microbiology

authors： Bent E,Tuzun S,Chanway CP,Enebak S

更新日期：2001-09-01 00:00:00

abstract：:Attempts at improving chemical fixation for electron-microscopic observation of the capsule of Klebsiella pneumoniae were made. The capsule was preserved by using alcian blue - lanthanum and tris-(1-aziridinyl) phosphine oxide (TAPO) - aldehyde - osmium procedures. Despite the different retention of the overall capsul...

journal_title：Canadian journal of microbiology

authors： Cassone A,Garaci E

更新日期：1977-06-01 00:00:00