Real-time polymerase chain reaction for detection of encapsulated Haemophilus influenzae using degenerate primers to target the capsule transport gene bexA.

abstract：:This study compared the recovery of Cryptosporidium oocysts and Giardia cysts ((oo)cysts) from raw waters using 4 different concentration-elution methods: flatbed membranes, FiltaMax foam, Envirochek HV capsules, and Hemoflow ultrafilters. The recovery efficiency of the combined immunomagnetic separation and staining ...

journal_title：Canadian journal of microbiology

authors： Ferguson C,Kaucner C,Krogh M,Deere D,Warnecke M

更新日期：2004-09-01 00:00:00

abstract：:Pinus monticola antimicrobial peptide 1 (Pm-AMP1) was expressed and purified from bacterial cell lysate and its identity and purity confirmed by Western blot analysis using the Pm-AMP1 antibody. Application of Pm-AMP1 resulted in visible hyphal growth inhibition of Cronartium ribicola , Phellinus sulphurascens , O...

journal_title：Canadian journal of microbiology

authors： Zamany A,Liu JJ,Ekramoddoullah A,Sniezko R

更新日期：2011-08-01 00:00:00

abstract：:Diversity of actinomycetes and their antifungal activities against some plant pathogenic fungi were examined in various vegetative soils from 14 different sites in the western part of Korea. Actinomycete counts ranged from 1.17 x 10(6) to 4.20 x 10(6) cfu x g(-1) dried soil. A total of 1510 actinomycetes were isolated...

journal_title：Canadian journal of microbiology

authors： Lee JY,Hwang BK

更新日期：2002-05-01 00:00:00

abstract：:Approximately 20% of Canada's oil supply now comes from the extraction of bitumen from the oil sands deposits in northeastern Alberta. The oil sands are strip-mined, and the bitumen is typically separated from sand and clays by an alkaline hot water extraction process. The rapidly expanding oil sands industry has mill...

journal_title：Canadian journal of microbiology

authors： Fedorak PM,Coy DL,Salloum MJ,Dudas MJ

更新日期：2002-01-01 00:00:00

abstract：:Fusaproliferin (FP), a toxic metabolite of the world-wide maize pathogens Fusarium proliferatum and Fusarium subglutinans, was recently found to be a natural contaminant of maize. Its toxic activity on haematopoietic human cell lines and its teratogenic effects on chicken embryos has been recently proved. Therefore a ...

journal_title：Canadian journal of microbiology

authors： Monti SM,Fogliano V,Randazzo G,Peluso G,Logrieco A,Ritieni A

更新日期：1999-01-01 00:00:00

abstract：:Rhizobacteria promote and have beneficial effects on plant growth, making them useful to agriculture. Nevertheless, the rhizosphere of the chickpea plant has not been extensively examined. The aim of the present study was to select indole-3-acetic acid (IAA) producing rhizobacteria from the rhizosphere of chickpea pla...

journal_title：Canadian journal of microbiology

authors： Fierro-Coronado RA,Quiroz-Figueroa FR,García-Pérez LM,Ramírez-Chávez E,Molina-Torres J,Maldonado-Mendoza IE

更新日期：2014-10-01 00:00:00

abstract：:Several probes for measuring dissolved carbon dioxide (CO2) concentration were installed in a 68-litre fermentor and their effectiveness compared. Submerged silastic rubber tubing gave reproducible results over a wide range of operating conditions and was generally superior to all other probes evaluated. The silastic ...

journal_title：Canadian journal of microbiology

authors： Alford JS Jr

更新日期：1976-01-01 00:00:00

abstract：:The open reading frame pp0053, which has a high homology with the sequence of mitochondrial sulfide dehydrogenase (HMT2) conferring cadmium tolerance in fission yeast, was amplified from Pseudomonas putida KT2440 and expressed in Escherichia coli JM109(DE3). The isolated and purified PP0053-His showed absorption spect...

journal_title：Canadian journal of microbiology

authors： Shibata H,Kobayashi S

更新日期：2006-08-01 00:00:00

abstract：:Pseudomonas aeruginosa ATCC 9027 grew on 0.5% (v/v) hexadecane as a sole carbon source in a chemically defined medium which required the addition of Fe3+ and Ca2+. There was a variable and extended lag period before an active growth rate was attained. Visible light microscopic evidence revealed that the bacteria did n...

journal_title：Canadian journal of microbiology

authors： Miguez CB,Beveridge TJ,Ingram JM

更新日期：1986-03-01 00:00:00

abstract：:The lytic effect of lysozyme on Streptococcus faecalis ATCC 9790 was studied by spectrophotometry and electron microscopy and it was found to be highly dependent on the ionic strength of the suspending media and on the ratio lysozyme to bacterial cell mass. When 7.2 X 10(8) bacteria/mL are exposed to 0.4 mg/mL of lyso...

journal_title：Canadian journal of microbiology

authors： Carvalho ME,Gonçalves MH,Silva MT

更新日期：1984-07-01 00:00:00

abstract：:Experiments were conducted to examine the dynamics of Cache Valley virus in Culiseta inornata, the probable chief vector of the virus. Of about 1500 laboratory reared C. inornata exposed to viraemic suckling mice, 72 took a blood meal. A relatively high precentage (93%) of the latter mosquitoes became infected. The vi...

journal_title：Canadian journal of microbiology

authors： Hayles LB,Lversen JO

更新日期：1980-03-01 00:00:00

abstract：:The purpose of this study was to determine the prevalence of antibiotic-resistant bacteria and endotoxin in soil after land application of biosolids. Soil was collected over a 15 month period following land application of biosolids, and antibiotic resistance was ascertained using clinically relevant antibiotic concent...

journal_title：Canadian journal of microbiology

authors： Brooks JP,Maxwell SL,Rensing C,Gerba CP,Pepper IL

更新日期：2007-05-01 00:00:00

abstract：:The oomycetes, a distinct phylogenetic lineage of fungus-like microorganisms, are heterokonts (stramenopiles) belonging to the supergroup Chromalveolata. Although the complete genomic sequences of a number of oomycetes have been reported, little information regarding the introns therein is available. Here, we investig...

journal_title：Canadian journal of microbiology

authors： Shen D,Ye W,Dong S,Wang Y,Dou D

更新日期：2011-02-01 00:00:00

abstract：:A large pool of antibiotic resistant and auxotrophic mutants was isolated from the Staphylococcus aureus phage group 2 strains UT0002-19 and UT0017 by (1) antibiotic gradient plates, (2) trimethoprim selection, and (3) nitrosoguanidine mutagenesis, which sometimes was coupled by enrichment with either penicillin or me...

journal_title：Canadian journal of microbiology

authors： Martin SM,Rogolsky M

更新日期：1980-08-01 00:00:00

abstract：:Fifty-four strains of Neisseria gonorrhoeae were examined for their ability to be agglutinated by various lectins. Wheat germ agglutinin, ricin, soybean lectin, and peanut agglutinin agglutinated all strains tested. Dolichos biflorus, Sophora japonica, Maclura pomifera, Ulex Europaeus, Lens culinaris, Canavalia ensifo...

journal_title：Canadian journal of microbiology

authors： Allen PZ,Connelly MC,Apicella MA

更新日期：1980-04-01 00:00:00

abstract：:The initial cause of the cessation of patulin biosynthesis in submerged cultures of Penicillium urticae was sought. The extrinsic limitations to patulin biosynthesis in fermentor cultures were successfully removed and kinetic profiles of the metabolites of the pathway and of three pathway enzymes were determined over ...

journal_title：Canadian journal of microbiology

authors： Neway J,Gaucher GM

更新日期：1981-02-01 00:00:00

abstract：:The role the environment has on populations of Pythium and Mesocriconema spp. was investigated at 5 golf course locations in east central Alabama. Every 4 to 5 weeks soil samples were collected from 3 golf greens on each of the 5 golf courses. Environmental data, including air and soil temperature, pH and relative hum...

journal_title：Canadian journal of microbiology

authors： Allen TW,Han DY,Bowen KL

更新日期：2005-04-01 00:00:00

abstract：:Despite the interactions known to occur between various lower respiratory tract pathogens and alveolar epithelial cells (AECs), few reports examine factors influencing the interplay between Mycobacterium tuberculosis bacilli and AECs during infection. Importantly, in vitro studies have demonstrated that the M. tubercu...

journal_title：Canadian journal of microbiology

authors： Pavlicek RL,Fine-Coulson K,Gupta T,Quinn FD,Posey JE,Willby M,Castro-Garza J,Karls RK

更新日期：2015-12-01 00:00:00

abstract：:Seventeen of 22 group A meningococcal strains were separated into four serotypes by a microbactericidal assay using group A antisera. In addition, 6 of 19 of these strains were killed by a group B antiserum and similar cidal patterns were obtained with a group C antiserum. There was little correlation between type and...

journal_title：Canadian journal of microbiology

authors： Quan AL,Sippel JE

更新日期：1979-08-01 00:00:00

abstract：:Exogenous nucleotides were found to protect mammalian cells from the lethal effects of diphtheria toxin. Protective potency of a given nucleotide was base specific and phosphate chain length dependent. Full expression of protective potency required an intact nucleotide, but the effect did not appear to be mediated by ...

journal_title：Canadian journal of microbiology

authors： Middlebrook JL,Dorland RB

更新日期：1979-03-01 00:00:00

abstract：:A series of synthetic promoters, based upon the Escherichia coli sigma 70 consensus promoter sequence, was constructed upstream of the lacZ reporter gene in the modified broad-host-range vector pQF52. The role of the intervening spacer region in gene expression in Pseudomonas aeruginosa and E. coli was studied by inse...

journal_title：Canadian journal of microbiology

authors： McLean BW,Wiseman SL,Kropinski AM

更新日期：1997-10-01 00:00:00

abstract：:Numbers of viable bacteria as indicated by colony-forming units (CFU), microbial adenosine triphosphate levels, dissolved organic carbon (DOC), particulate organic carbon and nitrogen (POC and PON), glucose concentration, heterotrophic potential (Vmax), and in situ heterotrophic activity (Vn) of near-surface microlaye...

journal_title：Canadian journal of microbiology

authors： Dietz AS,Albright LJ,Tuominen T

更新日期：1976-12-01 00:00:00

abstract：:The morphology of Clostridium pasteurianum cells grown on 10(-2) M SO32- showed significant alteration in cell shape and the absence of the electron translucent reserve polysaccharide (amylopectin) when compared to sulfate-grown cells. At the lower sulfite concentrations (10(-3) and 10(-4)M SO32-) the cells showed the...

journal_title：Canadian journal of microbiology

authors： McCready RG,Costerton JW,Laishley EJ

更新日期：1976-02-01 00:00:00

abstract：:Haemophilus paragallinarum secretes metalloproteases into different culture media lacking serum. Secreted proteins, concentrated by precipitation with 70% ammonium sulphate ((NH(4))(2)SO(4)) or methanol, displayed proteolytic activity at >100 kDa molecular mass in 10% polyacrylamide gels co-polymerized with porcine ge...

journal_title：Canadian journal of microbiology

authors： Rivero-García PC,Cruz CV,Alonso PS,Vaca S,Negrete-Abascal E

更新日期：2005-10-01 00:00:00

abstract：:Probiotics are usually isolated from the gastrointestinal tract of humans and animals. The search of probiotics in human milk is a recent field of research, as the existence of the human milk microbiome was discovered only about a decade ago. To our knowledge, no reports regarding the potential probiotic effect of bac...

journal_title：Canadian journal of microbiology

authors： Quilodrán-Vega SR,Villena J,Valdebenito J,Salas MJ,Parra C,Ruiz A,Kitazawa H,García A

更新日期：2016-06-01 00:00:00

abstract：:Fecal coliforms (FC) counts were compared with Escherichia coli counts in differently contaminated freshwater samples (n = 166). FC were enumerated by plate count on triphenyl 2,3,5-tetrazolium chloride Tergitol medium. Escherichia coli were enumerated by the most probable number microplate method based on the detecti...

journal_title：Canadian journal of microbiology

authors： Garcia-Armisen T,Prats J,Servais P

更新日期：2007-06-01 00:00:00

abstract：:Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...

journal_title：Canadian journal of microbiology

authors： Figueira MM,Ciminelli VS,de Andrade MC,Linardi VR

更新日期：1996-05-01 00:00:00

abstract：:The results of sucrose-density-gradient centrifugation of a cell-free particulate enzyme system from Acetobacter xylinum which was incubated with uridine diphosphoglucose indicate that there is a polymeric intermediate in the biosynthesis of cellulose. This intermediate has the properties of an oligomer of glucose, is...

journal_title：Canadian journal of microbiology

authors： Kjosbakken J,Colvin JR

更新日期：1975-02-01 00:00:00

abstract：:The aim of this study was to determine possible differences in the faecal microbiota of dairy cows infected with Mycobacterium avium subsp. paratuberculosis (Johne's disease) in comparison with noninfected cows from the same herds. Faecal samples from cows in 4 herds were tested for M. avium subsp. paratuberculosis by...

journal_title：Canadian journal of microbiology

authors： Kaevska M,Videnska P,Sedlar K,Bartejsova I,Kralova A,Slana I

更新日期：2016-06-01 00:00:00

abstract：:Sera from mice which have been vaccinated with BCG and challenged with old tuberculin contain gamma interferon. These same sera also express antibacterial activity. Using Staphylococcus aureus we demonstrated that its growth was inhibited at dilutions of sera as high as 1:320. A 4% concentration of sera reduced the gr...

journal_title：Canadian journal of microbiology

authors： Moulton RG,Gerner GD

更新日期：1986-05-01 00:00:00