Abstract:
:The repair of DNA damage caused by ultraviolet radiation (UVR) is well understood in both lower and higher organisms. Genetic studies carried out at optimum temperature for growth, 37 °C in Escherichia coli, have revealed the major pathways of DNA repair. We show that E. coli cells grown at 20 °C are more sensitive to UVR than cells grown at 37 °C. The analysis of knockout mutants demonstrates that cells impaired in recombinational DNA repair pathways show increased UV sensitivity at 20 °C. Cells with mutations in the nucleotide excision repair (NER) pathway genes are highly sensitive to UVR when grown at 37 °C and retain that sensitivity when grown at 20 °C, whereas wild-type cells are not sensitive when grown at 37 °C but become more sensitive to UVR when grown at low temperatures. Our results taken along with reports from the literature suggest that the UVR sensitivity of E. coli cells at low temperature could be due to impaired NER function.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Mangoli S,Rath D,Goswami M,Jawali Ndoi
10.1139/cjm-2013-0874subject
Has Abstractpub_date
2014-05-01 00:00:00pages
327-31issue
5eissn
0008-4166issn
1480-3275journal_volume
60pub_type
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