Abstract：
:A 3.1-kb region of the bacteriophage D3 genome which contains the immunity functions has recently been sequenced (GenBank accession No. L22692). Sequence analysis indicated the presence of a putative repressor gene (c1) whose protein product functions to maintain the bacteriophage genome as a stably integrated prophage in the chromosome of Pseudomonas aeruginosa. A plasmid was constructed that overexpresses repressor C1 protein under control of P(tac) in Escherichia coli. C1 protein was subsequently purified and characterized as a 223 amino acid protein with specific binding affinity for 14-base imperfect palindromic operator sequences located on the genome of bacteriophage D3. N-terminal protein sequence data obtained from automated Edman degradation (16 cycles) of purified repressor protein were identical to the predicted sequence based on DNA sequence analysis of the c1 open reading frame.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Farinha MA,Kropinski AMdoi
10.1139/m97-030subject
Has Abstractpub_date
1997-03-01 00:00:00pages
220-6issue
3eissn
0008-4166issn
1480-3275journal_volume
43pub_type
杂志文章abstract：:Neisseria polysaccharea (LNP 462, NCTC 11858), proposed as a prototype strain constituting a new taxon in the genus Neisseria, produces copious amounts of polysaccharide when grown on agar containing 1-5% sucrose. Plate-grown cells produced an exocellular polysaccharide which was composed of D-glucose, had [alpha]D +2...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-167
更新日期：1986-12-01 00:00:00
abstract：:Isolated toluene-degrading Pseudomonas stutzeri ST-9 bacteria were grown in a minimal medium containing toluene (100 mg·L(-1)) (MMT) or glucose (MMG) as the sole carbon source, with specific growth rates of 0.019 h(-1) and 0.042 h(-1), respectively. Scanning (SEM) as well as transmission (TEM) electron microscope anal...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0099
更新日期：2016-08-01 00:00:00
abstract：:The study of the relationship between MVL 51 virus, of the Gourlay group L 1, and the Mycoplasma cells of A. laidlawii during the first 35 min of infection leads to the conclusion that a "carrier state" has been established, free virus as well as infected and noninfected cells being present at the same time in the cul...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:Using a mixture of three mono nitrophenols as sole carbon, nitrogen and energy sources, mixed cultures were enriched from municipal activated sludge to degrade both nitrophenols and nitrobenzene. Bacterial growth and degradation rate could be increased by supplementing the medium with 0.1% YE. Microorganisms were isol...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:Polymyxin-resistant (PBLr) mutants of Agrobacterium tumefaciens A6, B6, and B6M were isolated from polymyxin-sensitive (PBLs) parent strains in a defined medium containing 600 microgram of polymyxin B sulfate per millilitre. The weight and number of tumors induced by PBLr mutants on a variety of host plants such as ca...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-029
更新日期：1979-02-01 00:00:00
abstract：:The fatty acid pattern in Mycobacterium vaccae strain JOB5 was examined after growth on n-alkanes (C14-C18), 1-alkenes (C14-C18), 2- or 3-methyl octadecane, and 8-heptadecene. It was evident that monoterminal oxidation of n-alkanes was followed by beta-oxidation and that both parent fatty acid and products of beta-oxi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-012
更新日期：1975-01-01 00:00:00
abstract：:Roots of field-grown tropical maize, Panicum maximum Jacq. and Digitaria decumbens Stent., and of sorghum and wheat grown in monoxenic culture with the diazotroph Spirillum lipoferum (syn. Azospirillum spp.) were examined for tetrazolium-reducing bacteria following incubation of roots in a malate-phosphate buffer-2,3,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-122
更新日期：1978-06-01 00:00:00
abstract：:It is important to understand the dynamics of tetracycline-resistant bacteria (TRB) and tetracycline resistance genes (TRGs) in bulk and rhizosphere soils for evaluating the spread of TRGs from pig manure to human. In this work, a greenhouse experiment was conducted to investigate the difference in abundance of TRB, t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0767
更新日期：2017-07-01 00:00:00
abstract：:We compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (Helicoverpa armigera), using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences and 16S library sequence analysis. DGGE profiles and 16S rDNA library se...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-064
更新日期：2006-11-01 00:00:00
abstract：:The induction of antituberculous immunity by crude ribosomal fractions isolated from Mycobacterium tuberculosis strain H37Ra, M. bovis strain BCG, and M. smegmatis was studied in CF-1 mice. Levels of antituberculous immunity similar to that induced by live BCG were induced by the BCG and H37Ra ribosomal fractions wher...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-032
更新日期：1983-02-01 00:00:00
abstract：:An active in vitro protein-synthesizing system has been developed from Rhodospirillum rubrum grown under either photosynthetic or heterotrophic conditions. A protease activity has been found in both of these systems, and this activity can be readily inactivated by treating the cells with KCl and phenylmethyl sulfonylf...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-042
更新日期：1976-02-01 00:00:00
abstract：:An investigation of brucellosis caused by Brucella suis, type 4, in reindeer, Rangifer tarandus L., and other ungulates inoculated experimentally with virulent isolates was undertaken to observe the course of infection, follow titres of serum agglutins, and determine the extent to which intraspecific and interspecific...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-024
更新日期：1978-02-01 00:00:00
abstract：:Colletotrichum gloeosporioides is the causal agent of rubber tree anthracnose and leads to serious losses of natural rubber production. The pathogenesis of C. gloeosporioides on rubber tree remains unknown. Cerato-platanin proteins are small, secreted cysteine-rich proteins that contribute to virulence and function in...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0087
更新日期：2018-11-01 00:00:00
abstract：:New antibiotics produced by Streptomyces echinatus A8331 cultured in the presence of heterocyclic aromatic acids can be separated and purified by high-performance liquid chromatography using reversed phase columns. Natural quinoxaline antibiotics and certain quinoline derivatives can also be efficiently separated in n...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-112
更新日期：1984-06-01 00:00:00
abstract：:Fetal bovine serum (FBS) is a necessary constituent of the culture media employed to foster the growth of Trypanosoma cruzi epimastigote forms. In different laboratories, the serum is used at final concentrations of 5 or 10%. We have normally supplemented the complex medium with 10% FBS. Under this condition we have d...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-132
更新日期：1995-10-01 00:00:00
abstract：:Like recF and recQ mutants studied earlier, two other classes of Escherichia coli mutants defective in the RecF conjugal recombination pathway, recJ and recO, were found to be partially resistant to thymineless death. In contrast, a recN mutant, also belonging to the pathway, was indistinguishable from the wild type w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-157
更新日期：1988-07-01 00:00:00
abstract：:Pathogenic strains of Vibrio cholerae O139 possess the cholera toxin A subunit (ctxA) gene as well as the gene for toxin co-regulated pili (tcpA). We report the isolation of a ctxA-negative, tcpA-negative V. cholerae O139 strain (INDREI) from a patient in Mexico diagnosed with gastrointestinal illness. Certain phenoty...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-004
更新日期：2003-01-01 00:00:00
abstract：:The altitudinal distribution of microfungi associated with Betula ermanii Cham. leaf litter was investigated on Mt. Rishiri (1719 m), northern Japan. Fallen leaves were collected at five sites of different altitudes (approximately 300, 600, 900, 1200, and 1500 m above sea level) along a transect on the northern slope ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-030
更新日期：2009-07-01 00:00:00
abstract：:In the dairy industry, bacterial contaminants persist on equipment surfaces due to spore and biofilm formation. These are involved in cross-contamination problems that affect the quality of processed products and limit their life. The pasteurization process, in which milk is submitted to moderate heat treatment, is in...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/cjm-2018-0435
更新日期：2019-06-01 00:00:00
abstract：:Ten bacterial strains were isolated by enrichment culture, using as carbon sources either aliphatics or an aromatic-polar mixture. Oxygen uptake rate was used as a criterion to determine culture transfer timing at each enrichment stage. Biodegradation of aliphatics (10,000 mg L(-1)) and an aromatic-polar mixture (5000...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-098
更新日期：2003-12-01 00:00:00
abstract：:Four modes of heating endotoxin in plasma and two different times of heating endotoxin in pyrogen-free water were compared. There were no significant differences in standard curves after heating endotoxin in plasma at 100 degrees C for 1 and for 10 min. However, the standard curve after heating for 10 min at 75 degree...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-140
更新日期：1986-10-01 00:00:00
abstract：:The aim of this study was to assess the influence of sodium content on the microbiota on the surface of ready-to-eat (RTE) meat products purchased from the retail market in Canada. Products, including sliced and sausage-type deli meats, were analysed with culture-dependent and culture-independent methods. Bacteria wer...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0630
更新日期：2015-02-01 00:00:00
abstract：:Filamentous growth of Candida albicans occurs in response to a variety of environmental signals. The C. albicans gene orf19.1944 and its allele orf19.9499 are identical and are predicted to encode an 823-residue, 7-transmembrane-domain protein that has all the expected features of a G-protein-coupled receptor. The pro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-095
更新日期：2004-12-01 00:00:00
abstract：:Marine shrimp of the species Penaeus monodon were collected from the coastal region (Haroa) of the deltaic Sundarbans of West Bengal, India during the premonsoon period in 1996. Klebsiella pneumoniae was isolated from the alimentary canal and gills of the shrimp as the sole isolate. All 10 isolated strains were resist...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:Influenza undergoes extensive antigenic variation in nature. These new antigenic variants invariably supplant the preceding antigenic type that then disappears. In apparent contrast to this, two H3N2 strains that were forwarded to the Laboratory Centre for Disease Control, Ottawa, in 1984 from a Canadian public health...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-139
更新日期：1987-09-01 00:00:00
abstract：:This study aimed to examine aminoglycosides (AMGs) resistance mechanisms, including the AMG-modifying enzyme genes, mexXY, rplY, nuoG, and galU, in the Pseudomonas aeruginosa non-cystic fibrosis (CF) isolates in Thailand. One hundred P. aeruginosa isolates from non-CF patients were examined for susceptibility to AMGs ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0465
更新日期：2013-01-01 00:00:00
abstract：:From the four known isoforms of the staphylococcal exfoliative toxins (ETs), only ETA and ETB are the major causative agents. General knowledge is that the gene for ETA is located on the chromosome, whereas that for ETB is located on a large plasmid. Yoshizawa and co-workers (2000, Microbiol. Immunol. 44(3): 189-191) ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0721
更新日期：2013-04-01 00:00:00
abstract：:In a previous study it was shown that the number of wood-inhabiting bacteria was drastically reduced after colonization of beech (Fagus sylvatica) wood blocks by the white-rot fungus Hypholoma fasciculare, or sulfur tuft (Folman et al. 2008). Here we report on the mechanisms of this fungal-induced antibacterial activi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-023
更新日期：2010-05-01 00:00:00
abstract：:A bacterial strain capable of utilizing 3,6-dimethylphenanthrene (3,6-DMP) as its sole source of carbon and energy was isolated from a creosote-contaminated soil. The isolate was identified as a strain of Sphingomonas sp. and was designated strain JS1. Utilization of 3,6-DMP was demonstrated by an increase in bacteria...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-018
更新日期：2003-02-01 00:00:00
abstract：:The phototrophic purple sulfur bacterium Thiopedia rosea forms multicellular, gas-vacuolate, regular, flat aggregates (platelets, sheets) held together by slime. Platelets found in eutrophic water consisted of slime (85% of the total wet volume) and 16 cells, while the gas-filled vacuole occupied 44% of the volume of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-113
更新日期：1986-07-01 00:00:00
