Abstract：
:The fatty acid pattern in Mycobacterium vaccae strain JOB5 was examined after growth on n-alkanes (C14-C18), 1-alkenes (C14-C18), 2- or 3-methyl octadecane, and 8-heptadecene. It was evident that monoterminal oxidation of n-alkanes was followed by beta-oxidation and that both parent fatty acid and products of beta-oxidation were incorporated into cellular lipids. Radioactive experiments demonstrated that there was desaturation of long-chain fatty acids. There was no evidence of chain elongation. Growth on 1-alkenes resulted in the incorporation of fatty acids that were products of two primary modes of oxidation: (1) methyl group attack resulting in omega-unsaturated fatty acids and (2) double-bond attack resulting in the removal of one carbon from the substrate. Cells of strain JOB5 grown on 2- and 3-methyl octadecane contained the corresponding iso- or anteiso-fatty acids in significant quantity. Cells cultured on 8-heptadecene contained 8- and 9-heptadecenoic acids, 6- and 7-pentadecenoic acids, 9- and 10-methyl heptadecanoic acids, and 7- and 8-methyl pentadecanoic acids. Fatty acid composition (C13 to C19) was affected by substrate chain length and was additionally modified by cellular control mechanisms.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
King DH,Perry JJdoi
10.1139/m75-012subject
Has Abstractpub_date
1975-01-01 00:00:00pages
85-9issue
1eissn
0008-4166issn
1480-3275journal_volume
21pub_type
杂志文章abstract：:Marine shrimp of the species Penaeus monodon were collected from the coastal region (Haroa) of the deltaic Sundarbans of West Bengal, India during the premonsoon period in 1996. Klebsiella pneumoniae was isolated from the alimentary canal and gills of the shrimp as the sole isolate. All 10 isolated strains were resist...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:We studied the persistence of naturally acquired cell-mediated immunity to rubella during early pregnancy. We compared lymphocyte transformation responses to phytohemagglutinin and rubella virus in 35 naturally immune women aged 17-37 years, in the first trimester of pregnancy, with 49 naturally immune age-matched non...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-257
更新日期：1983-12-01 00:00:00
abstract：:Various soil fungi were tested for their capacity to degrade the insecticide [14C]photodieldrin. Of nine species investigated, Trichoderma viride was the only one which degraded the insecticide to an appreciable extent into water-soluble, non-insecticidal compounds within 4-5 weeks. These products amounted to 32-41% o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-198
更新日期：1976-09-01 00:00:00
abstract：:Ribosomal preparations from Neisseria gonorrhoeae types 1 and 4 were examined for their in vitro stimulation of mouse splenocytes to determine the ribosomal moiety or contaminant responsible for the immunoproliferative activity. In immunodiffusion tests with homologous rabbit antiserum, crude 70S ribosomes formed four...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-094
更新日期：1978-05-01 00:00:00
abstract：:Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and th...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-236
更新日期：1978-12-01 00:00:00
abstract：:Five strains of Haemophilus pleuropneumoniae, out of eight strains tested, produced extracellular haemolysin(s) when grown in liquid culture in the presence, but not in the absence, of RNA. The haemolysin produced by the neotype strain was unstable, heat labile, and sensitive to degradation by pronase, trypsin, and ch...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-085
更新日期：1985-05-01 00:00:00
abstract：:Polymerase chain reaction amplification was used to compare different regions of the cytochrome c3 gene from nine strains of Desulfovibrio vulgaris, to examine homology within the species. Six 30-base polymerase chain reaction primers and three probes were synthesized on the basis of the published nucleic acid sequenc...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-059
更新日期：1993-04-01 00:00:00
abstract：:Andosols comprise one of the most important soil groups for agricultural activities in Japan because they cover about 46.5% of arable upland fields. In this soil group, available phosphorus (P) is accumulated by application of excessive fertilizer, but little is known about the influence of increasing P availability o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0612
更新日期：2013-06-01 00:00:00
abstract：:The phototrophic purple sulfur bacterium Thiopedia rosea forms multicellular, gas-vacuolate, regular, flat aggregates (platelets, sheets) held together by slime. Platelets found in eutrophic water consisted of slime (85% of the total wet volume) and 16 cells, while the gas-filled vacuole occupied 44% of the volume of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-113
更新日期：1986-07-01 00:00:00
abstract：:The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1-2 d. In the buffer system, the 1- and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-131
更新日期：2005-03-01 00:00:00
abstract：:Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (DNA-DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C)) and relative genome sizes were determined for selected strains...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-083
更新日期：1979-05-01 00:00:00
abstract：:Two rod-shaped viruses of similar length occurring in hops were isolated from a mixed infection. One (hop mosaic virus) infected Nicotiana clevelandii, produced mosaic symptoms in mosaic-sensitive hop cultivars, and did not infect Chenopodium murale. The other (hop latent virus) infected C. murale but not N. cleveland...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-168
更新日期：1976-08-01 00:00:00
abstract：:The cultural, morphological, and physiological properties of Thermomonospora fusca (strain 190Th) are described. Its physiological properties show that this species is primarily a carbohydrate-degrading actinomycete which can use a wide range of plant sugars and polymeric carbohydrates as sources of carbon and energy....
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-267
更新日期：1975-11-01 00:00:00
abstract：:Characterization and enumeration of the adherent epimural community of the rumen wall of young, conventionally reared lambs were carried out from 2 to 21 days after birth. Three hundred strains were isolated by anaerobic procedures from three sites: dorsal, ventral, and caudal sacs, and from the sloughed epithelial ce...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-114
更新日期：1989-07-01 00:00:00
abstract：:The bacterial flora of an experimental plant that processes liquid swine manure by an aerated compartmented (multistage) lagoon system has been studied. The total flora is characterized by a larger number of oligotrophic bacteria than eutrophic ones. Each compartment displays a specific flora, different from the flora...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-154
更新日期：1997-11-01 00:00:00
abstract：:We examined isolates from 4 commercial bioinsecticides based on different strains of Bacillus thuringiensis subspecies (kurstaki, israelensis, aizawai, and tenebrionis) for the presence of genes encoding proteins with known enterotoxigenicity (nhe, hbl, cytk, ces) and various other putative virulence genes (piplc, sph...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-106
更新日期：2007-12-01 00:00:00
abstract：:Two-dimensional (2-D) polyacrylamide gel electrophoresis was used to detect proteins induced in Frankia sp. strain ACN14a-tsr by root exudates of its symbiotic host, Alnus glutinosa. The 5 most prominent proteins were purified from 2-D gels and characterized by N-terminal sequencing. All of these proteins had a high p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-046
更新日期：2001-06-01 00:00:00
abstract：:In this work a bacterial classification method based on the discriminant analysis of the microcalorimetric data provided by the growth power-time (p-t) curves is developed. This method is applied to classify several species of Enterobacteria of different origins, and the results are compared with those obtained by con...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-186
更新日期：1988-09-01 00:00:00
abstract：:We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-102
更新日期：2007-01-01 00:00:00
abstract：:The presence of other soil microorganisms might influence the ability of rhizobacterial inoculants to promote plant growth either by reducing contact between the inoculant and the plant root or by interfering with the mechanism(s) involved in rhizobacterially mediated growth promotion. We conducted the following exper...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-080
更新日期：2001-09-01 00:00:00
abstract：:Viruses were isolated from every sample of raw (100 L) and treated (1000 L) water collected at a water treatment plant drawing sewage-contaminated river water. Few plaque-forming isolates were formed but cytopathogenic viruses were isolated as frequently in drinking water as in raw water. In drinking water some sample...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-063
更新日期：1981-04-01 00:00:00
abstract：:The gene-encoding HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus JCM2501 was amplified and expressed in Escherichia coli and the enzymatic features were examined. The enzyme was detected mainly in the membrane fraction. It catalyzed the sulfide-dependent menaquinone (MK) reduction showing special enz...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-077
更新日期：2007-09-01 00:00:00
abstract：:Fifty-two killer, factor-resistant, nuclear mutants were isolated from sensitive strains of yeast and assorted into three functional groups. All but one mutant owed their resistance to an alteration in the cell wall binding site for killer. In several mutant strains, an alteration at the site of killer binding was ass...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-041
更新日期：1978-03-01 00:00:00
abstract：:The entire nucleotide sequence of the rsaA gene, encoding the paracrystalline surface (S) layer protein (RsaA) of Caulobacter crescentus CB15A, was determined. The rsaA gene encoded a protein of 1026 amino acids, with a predicted molecular weight of 98,132. Protease cleavage of mature RsaA protein and amino acid seque...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-033
更新日期：1992-03-01 00:00:00
abstract：:Four phages isolated from cattle and poultry feces were analyzed for their ability to lyse Salmonella serotypes and Escherichia coli O157:H7. The phage one-step growth curves, morphology, and genetic characteristics were determined. All phages showed a lytic effect on various Salmonella serotypes and E. coli O157:H7, ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-099
更新日期：2011-12-01 00:00:00
abstract：:The isolation of 12 strains of cellulolytic fungi from the rumen of a roughage-fed steer is described. These represented three different genera, including one new genus and species (Orpinomyces bovis). The organisms were indistinguishable on the basis of fermentation products from cellulose, and their fermentation pat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-090
更新日期：1990-07-01 00:00:00
abstract：:We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an indigenous infection with Escherichia coli (K. Nomoto, T. Yokokura, Y. Yoshikai, et al. Can. J. Microbiol. 37:244-247, 1991). In the present study, we demonstrate that nonspecific immunostimulation...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-126
更新日期：1992-08-01 00:00:00
abstract：:Alkaline phosphatase (APase) was present in the bovine rumen in both cell-free and cell-associated states and levels of the enzyme varied with dietary regime. Reaction product deposition showed that the enzyme was associated with the mixed bacterial population. No enzyme was observed to be associated with protozoa. Tr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-111
更新日期：1976-05-01 00:00:00
abstract：:The B subunit of Escherichia coli heat-labile enterotoxin (LTB), a nontoxic molecule with potent biological properties, is a powerful mucosal and parenteral adjuvant that induces a strong immune response against co-administered or coupled antigens. In this paper, the effect of LTB on the humoral immune response to rec...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-056
更新日期：2008-08-01 00:00:00
abstract：:The study investigated the presence of Borrelia-specific antibodies captured in immune complexes (ICs) in patients with early Lyme disease manifested by erythema migrans. Out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ICs containing IgM antibodies, while only 4 (22.2%) were IgG pos...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-105
更新日期：2007-12-01 00:00:00
