Effect of heat on endotoxin in plasma and in pyrogen-free water, as measured in the Limulus amoebocyte lysate assay.

Abstract:

:Four modes of heating endotoxin in plasma and two different times of heating endotoxin in pyrogen-free water were compared. There were no significant differences in standard curves after heating endotoxin in plasma at 100 degrees C for 1 and for 10 min. However, the standard curve after heating for 10 min at 75 degrees C had a significantly less steep slope, and after heating for 10 min at 56 degrees C, it was completely flat. Heating of endotoxin in pyrogen-free water for 1 min also resulted in the flattening of the standard curve, which was even more pronounced after 10 min of heating.

journal_name

Can J Microbiol

authors

Piotrowicz BI,McCartney AC

doi

10.1139/m86-140

subject

Has Abstract

pub_date

1986-10-01 00:00:00

pages

763-4

issue

10

eissn

0008-4166

issn

1480-3275

journal_volume

32

pub_type

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