Abstract:
:An endo-beta-1,4-xylanase gene was cloned from Neocallimastix patriciarum 27 in the bacteriophage vector lambda gtWES lambda B and was subcloned into the plasmid vectors pUC18 and pUC19 in which xylanase activity was expressed in both orientations. The xylanase was located in the periplasmic space of the host, Escherichia coli HB101. The pH and temperature optima for periplasmic xylanase activity were 6.2 and 40 degrees C, respectively, and the Km for oat spelt xylan hydrolysis was 0.89 mg.mL-1. It also exhibited hydrolytic activity on carboxymethyl cellulose that was equivalent to 28% of the activity exhibited by the enzyme on xylan. It bound to crystalline cellulose, but lacked hydrolytic activity on amorphous cellulose. SDS-PAGE followed by zymogram analysis showed active bands of 68, 58, and 51 kDa. Isoelectric focusing in gels combined with zymogram analysis showed one band of xylanase activity with a pI of 3.6.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Lee JM,Hu Y,Zhu H,Cheng KJ,Krell PJ,Forsberg CWdoi
10.1139/m93-020subject
Has Abstractpub_date
1993-01-01 00:00:00pages
134-9issue
1eissn
0008-4166issn
1480-3275journal_volume
39pub_type
杂志文章abstract::Bacterial biofilms are commonly formed on medical devices and food processing surfaces. The antimicrobials used have limited efficacy against the biofilms; therefore, new strategies to prevent and remove these structures are needed. Here, the effectiveness of brief oxidative treatments, based on the combination of sod...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0747
更新日期:2015-05-01 00:00:00
abstract::Nitrogenase in Azotobacter chroococcum whole cells was inhibited by enzymically generated superoxide anion (O2-), hydrogen peroxide, and ethyl hydrogen peroxide. The degree of inhibition produced by O2- was related to the quantity of oxygen supplied to the organisms in continuous cultures. O2- also inhibited oxygen up...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-228
更新日期:1977-11-01 00:00:00
abstract::This study was undertaken to determine if direct cytotoxicity (DC) against herpes simplex virus infected cells, perhaps mediated by T cells, could be demonstrated in individuals subject to recurrent herpes labialis. The mononuclear cells from 7 out of 17 individuals with recurrent herpes expressed DC whereas no DC was...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-177
更新日期:1978-09-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w98-224
更新日期:1999-03-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-194
更新日期:1978-10-01 00:00:00
abstract::Many studies on quorum quenching focus on the discovery and characterization of novel acyl-homoserine lactonases (AHL-lactonases) because these enzymes could be used in the control of diseases caused by Gram-negative bacteria. The effects of quorum quenching are also remarkable in the performance of bacterial consorti...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2017-01-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-122
更新日期:1994-09-01 00:00:00
abstract::Strain-specific fluorescent antibodies were prepared for three isolates of Aereomonas hydrophila. These antisera were reacted with 255 other A. hydrophila isolates, and their immunofluorescence was measured quantitatively by photometric fluorescence microscopy. Only 27.5% of the isolates reacted with the prepared anti...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-024
更新日期:1980-02-01 00:00:00
abstract::Our purpose was to study bacterial antagonism between a limited number of strictly anaerobic strains and Clostridium perfringens in the intestinal tract of gnotobiotic rodents. Gnotobiotic mice harboring a Bacteroides thetaiotaomicron, a Fusobacterium necrogenes, and a Clostridium sp. strain were protected against pat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m87-039
更新日期:1987-03-01 00:00:00
abstract::The antiserum agar method (ASA), which is based on the formation of immunoprecipitates around bacterial growth on agar containing meningococcal hyperimmune horse serum, was evaluated for serogroup identification of Neisseria meningitidis. Four hundred meningococcal stains were serogrouped by ASA employing horse antise...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-113
更新日期:1979-06-01 00:00:00
abstract::Fecal coliforms (FC) counts were compared with Escherichia coli counts in differently contaminated freshwater samples (n = 166). FC were enumerated by plate count on triphenyl 2,3,5-tetrazolium chloride Tergitol medium. Escherichia coli were enumerated by the most probable number microplate method based on the detecti...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-033
更新日期:2007-06-01 00:00:00
abstract::Environmental scanning electron microscopic observations of Postia placenta grown on a defined medium and on red spruce wood allowed for the examination of the hydrated sheath of P. placenta. In the wood environment, mature hyphae that were not adhering to the substrate were observed to have a mycofibrillar morphology...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-058
更新日期:1995-04-01 00:00:00
abstract::Through genomic analysis of mucosa-associated Escherichia coli strains, we found a close genetic association among isolates from pediatric inflammatory bowel disease (IBD) patients. A specific E. coli pathovar, adherent-invasive E. coli (AIEC), was found in Crohn's disease (CD) adult patients - this pathovar has enhan...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-007
更新日期:2012-04-01 00:00:00
abstract::A microplaque reduction assay for human and mouse interferons is described, using plastic disposable multicompartmental plates with flat-bottomed wells of 6 mm in diameter. The procedure is rapid and reproducible and lends itself to a considerable degree of automation. Its potential application in other areas of virol...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-186
更新日期:1975-08-01 00:00:00
abstract::Methanol extracts were obtained from L. interrogans serovars icterohaemorrhagiae and canicola and L. biflexa serovar patoc. Human sera from 167 normal individuals and 40 patients with different infectious diseases tested by complement fixation tests showed negative reactions. Sera from 100 patients with a suspicion of...
journal_title:Canadian journal of microbiology
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doi:10.1139/m91-075
更新日期:1991-06-01 00:00:00
abstract::Nitrogen-fixing, field-obtained root nodules of the silky green alder were studied by transmission electron microscopy. The nodule endophyte exhibited a prokaryotic cytology and was present in two forms: the hypha(0.3-1.0mum), which was branched and septate, and the vesicle (3-5mum), which was also septate and develo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-157
更新日期:1975-07-01 00:00:00
abstract::In both relA+ and relA- derivatives, the biosynthesis of peptidoglycan, lipid intermediates, and nucleotide precursors abruptly halted at the onset of diauxic lag from glucose to lactose with a concomitant accumulation of guanosine 5'-diphosphate 3'-diphosphate (ppGpp). These results are consistent with the proposal t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-188
更新日期:1979-10-01 00:00:00
abstract::The effect of the transition metal compound trans-[Rh(4-ethylpyridine)4Cl2]Cl x 2H2O on the syntheses of DNA, RNA, and protein has been investigated for an auxotrophic bacterial strain, Escherichia coli JS-1, incapable of thymidine, uridine, and histidine syntheses. At low concentration (7.4 x 10(-6) M), this rhodium ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-111
更新日期:1980-05-01 00:00:00
abstract::The origin of the eukaryotic nucleus is difficult to reconstruct. While eukaryotic organelles (chloroplast, mitochondrion) are eubacterial endosymbionts, the source of nuclear genes has been obscured by multiple nucleotide substitutions. Using evolutionary parsimony, a newly developed rate-invariant treeing algorithm,...
journal_title:Canadian journal of microbiology
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更新日期:1989-01-01 00:00:00
abstract::Twenty-six strains of group JK corynebacteria had the same colonial morphology and biological reactions as the biotypes of the biovars of Corynebacterium genitalium and C. pseudogenitalium. Therefore, group JK corynebacteria can be assigned to the biovars of C. genitalium or C. pseudogenitalium. Although the strains d...
journal_title:Canadian journal of microbiology
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更新日期:1984-08-01 00:00:00
abstract::There are inherent weaknesses associated with currently used bacterial fecal pollution indicator systems. Fecal pollution indicator data would be more meaningful if supplemented with information relating to the occurrence of pathogens in recreational water. Through surveys of four bathing beaches on Lake Ontario, it w...
journal_title:Canadian journal of microbiology
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doi:10.1139/m79-159
更新日期:1979-09-01 00:00:00
abstract::Staphylococcus aureus isolated from the cervix of an infertile woman was found to cause complete immobilization of human spermatozoa in vitro. Only the cell culture and cell-free supernatant showed immobilization activity, indicating that the sperm immobilization factor might be released extracellularly by the organis...
journal_title:Canadian journal of microbiology
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更新日期:2009-07-01 00:00:00
abstract::The effects of seven thiophosphorus pesticides and their degradation products on estuarine ammonium oxidizers were examined. Using an axenic, closed-culture, high cell density assay, the pesticides caused little inhibition of ammonium oxidation. However, the degradation product of methyl parathion, p-aminophenol, sign...
journal_title:Canadian journal of microbiology
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doi:10.1139/m80-216
更新日期:1980-11-01 00:00:00
abstract::Listeria monocytogenes and Listeria innocua differ markedly in virulence but are indistinguishable by classical taxonomic criteria. Both species are actively motile and produce abundant flagellin at 22 degrees C. We have found, however, noticeable differences between L. monocytogenes and L. innocua in motility and fla...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-076
更新日期:1995-07-01 00:00:00
abstract::The susceptibility of Cephalosporium acremonium to selected amino acid analogues was markedly influenced by the carbon source used in the test media. Lysine hydroxamate, beta-hydroxy norvaline, and hexafluorovaline were toxic when tested with ribose, ribose or fructose, and ribose or galactose, respectively. In contra...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-120
更新日期:1979-07-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-007
更新日期:1976-01-01 00:00:00
abstract::An active in vitro protein-synthesizing system has been developed from Rhodospirillum rubrum grown under either photosynthetic or heterotrophic conditions. A protease activity has been found in both of these systems, and this activity can be readily inactivated by treating the cells with KCl and phenylmethyl sulfonylf...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-042
更新日期:1976-02-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:2018-08-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-111
更新日期:1976-05-01 00:00:00