Abstract:
:This study was undertaken to determine if direct cytotoxicity (DC) against herpes simplex virus infected cells, perhaps mediated by T cells, could be demonstrated in individuals subject to recurrent herpes labialis. The mononuclear cells from 7 out of 17 individuals with recurrent herpes expressed DC whereas no DC was ever exhibited by 7 individuals without a previous history of herpes infections. Several approaches were used to show that the cytotoxicity being detected was predominately of the direct type rather than antibody-dependent cell cytotoxicity (ADCC). Since the effector cells of the DC were sensitive to trypsin treatment and behaved as do natural killer (NK) cells upon cell fractionation, the results were taken to imply that the DC was attributable to a NK-effector cell type rather than a classical T lymphocyte.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Fujimiya Y,Babiuk LA,Rouse BTdoi
10.1139/m78-177subject
Has Abstractpub_date
1978-09-01 00:00:00pages
1076-81issue
9eissn
0008-4166issn
1480-3275journal_volume
24pub_type
杂志文章abstract::Screening for mycotoxin production by Chaetomium spp. and related fungi on rice culture was conducted by a combination of cytotoxicity tests using HeLa cells and thin-layer chromatography. Producers of sterigmatocystin, O-methylsterigmatocystin, chaetochromin, chaetocin, chetomin, cochliodinols, and mollicellin G were...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-119
更新日期:1981-08-01 00:00:00
abstract::Pathogenic strains of Vibrio cholerae O139 possess the cholera toxin A subunit (ctxA) gene as well as the gene for toxin co-regulated pili (tcpA). We report the isolation of a ctxA-negative, tcpA-negative V. cholerae O139 strain (INDREI) from a patient in Mexico diagnosed with gastrointestinal illness. Certain phenoty...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-004
更新日期:2003-01-01 00:00:00
abstract::We continued our study of high-molecular-mass proteases (HMMPs) using several strains of the genus Trichoderma, and other filamentous fungi (Botrytis cinerea, Aspergillus niger, Fusarium culmorum, and Penicillium purpurogenum). We found that five Trichoderma strains secreted HMMPs into the media after induction with b...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0670
更新日期:2019-09-01 00:00:00
abstract::Glutaraldehyde, the biological fixative of choice in the cytochemical localization of the phosphatases, was investigated for its effects on Pseudomonas aeruginosa alkaline phosphatase. Comparative studies on the inactivation of alkaline phosphatase by glutaraldehyde showed significant differences when the purified pro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-078
更新日期:1981-05-01 00:00:00
abstract::Three composts (Ball, dairy, and greenhouse) were tested for the ability to suppress the development of Fusarium root and stem rot (caused by Fusarium oxysporum f. sp. radicis-cucumerinum) on greenhouse cucumber. Dairy and greenhouse composts significantly reduced disease severity (P = 0.05), while Ball compost had no...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-076
更新日期:2010-11-01 00:00:00
abstract::Surface oxidation of sulfides and copper (Cu) activation are 2 of the main processes that determine the efficiency of flotation. The present study was developed with the intention to ascertain the role of the phenomena in the biomodification of sulfides by Acidithiobacillus ferrooxidans culture (cells and growth me...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-072
更新日期:2012-09-01 00:00:00
abstract::The synthesis of the nucleotide precursors for peptidoglycan is regulated by the relA gene in Escherichia coli. Thus, nucleotide precursors labeled with [3H]diaminopimelic acid accumulated in a relA strain but not in an isogenic relA+ strain during amino acid deprivation. Furthermore, nucleotide precursor synthesis wa...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-098
更新日期:1982-06-01 00:00:00
abstract::The physiochemical characteristics of the intracellular proteolytic enzymes of Oidiodendron kalari, a neuropathogenic fungus, were studied. The organism in the yeast phase was grown in a semisynthetic medium containing 1% tryptone, at 37 degrees C for 48 hr, on a gyrotory shaker. The crude extract was prepared by brea...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-204
更新日期:1975-09-01 00:00:00
abstract::Among nine eluents tested, 0.5% (w/v) isoelectric casein at pH 9.0 and 0.5% (w/v) non-fat dry milk (pH 9.0) were the most efficient in eluting poliovirus type 1 (Sabin) from Eustis fine sand. However, no significant difference was found between the overall (elution followed by concentration) virus recoveries by non-fa...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-130
更新日期:1979-08-01 00:00:00
abstract::In this work, we disrupted one of three putative phosphatidylinositol phospholipase C genes of Aspergillus nidulans and studied its effect on carbon source sensing linked to vegetative mitotic nuclear division. We showed that glucose does not affect nuclear division rates during early vegetative conidial germination (...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-034
更新日期:2011-07-01 00:00:00
abstract::Like recF and recQ mutants studied earlier, two other classes of Escherichia coli mutants defective in the RecF conjugal recombination pathway, recJ and recO, were found to be partially resistant to thymineless death. In contrast, a recN mutant, also belonging to the pathway, was indistinguishable from the wild type w...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-157
更新日期:1988-07-01 00:00:00
abstract::alpha-Methyl lysine was investigated as a potential inhibitor of lysine transport in Escherichia coli and Bacillus sphaericus. At equimolar concentrations, no inhibition was observed in either organism, but at 10X and 100X the lysine concentration, alpha-methyl lysine caused a 20-50% reduction in the initial rate of l...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-095
更新日期:1986-06-01 00:00:00
abstract::Quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. The signal molecules involved in quorum sensing in P. carotovorum subsp. carotovorum belong to the group of N-acyl homoserine lactones (AHLs). In our study, we scre...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-062
更新日期:2006-10-01 00:00:00
abstract::We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in a dctA gene. Levels of dctA gene expression ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-008
更新日期:1992-01-01 00:00:00
abstract::The present investigation was aimed to scrutinize the salt tolerance potential of plant-growth-promoting rhizobacteria (PGPR) isolated from rhizospheric soil of selected halophytes (Atriplex leucoclada, Haloxylon salicornicum, Lespedeza bicolor, Suaeda fruticosa, and Salicornica virginica) collected from high-saline f...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0668
更新日期:2015-04-01 00:00:00
abstract::Dietary exposure to heavy metals may have detrimental effects on human and animal health, even at low concentrations. Specific probiotic bacteria may have properties that enable them to bind toxins from food and water. We assessed the interaction of probiotic bacteria with cadmium and lead in vitro as an initial scree...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-043
更新日期:2006-09-01 00:00:00
abstract::Bottles of medium were inoculated with both strains of Chromatiacea and Chlorobiaceae (mixed cultures) and immersed at differnt depths in the marine coastal waters of the Marseilles basin (France). After development, the in vivo absorption characteristics of the cells show the effect of the daylight penetration on the...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::Studies show that Paracoccus denitrificans can denitrify nitrogen sources under aerobic conditions. However, the lack of data on its genome sequence has restricted molecular studies and practical applications. In this study, the complete genome of P. denitrificans ATCC 19367 was sequenced and its nitrogen metabolism p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0037
更新日期:2019-07-01 00:00:00
abstract::A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-106
更新日期:1983-06-01 00:00:00
abstract::For the recently described serotype 15 of biotype I and serotypes 13 and 14 of biotype II of Actinobacillus pleuropneumoniae, fhuA and hgbA were detected by polymerase chain reaction and DNA sequencing. To determine the substrate specificity of the iron receptors FhuA and HgbA and to study their role in the virulence ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-135
更新日期:2006-04-01 00:00:00
abstract::Roots of field-grown tropical maize, Panicum maximum Jacq. and Digitaria decumbens Stent., and of sorghum and wheat grown in monoxenic culture with the diazotroph Spirillum lipoferum (syn. Azospirillum spp.) were examined for tetrazolium-reducing bacteria following incubation of roots in a malate-phosphate buffer-2,3,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-122
更新日期:1978-06-01 00:00:00
abstract::The search for new antimicrobial compounds involves finding novel sources of chemotherapeutic compounds or manipulating and combining structures from existing molecules. Small antimicrobial peptides (AMPs) are components of innate immune defenses characterized in greatest detail in insect-derived AMPs. We have generat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-001
更新日期:2009-05-01 00:00:00
abstract::Escherichia coli is capable of producing several virulence factors involved in the pathophysiology of bovine mastitis. Cell surface structures such as flagella, pili, and other outer membrane adhesins are considered essential for biofilm formation on abiotic surfaces. The aim of this study was characterize the molecul...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0053
更新日期:2013-05-01 00:00:00
abstract::Staphylococcus aureus was grown in a rich peptone medium which became alkaline with continued incubation. Cells were grown at 37 degrees C and in the same medium containing 1 M NaCl at 46 degrees C, a temperature at which this organism can grow only when protected by NaCl. Cells of these cultures are hereafter called ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-049
更新日期:1983-03-01 00:00:00
abstract::Urinary tract infections are common. Few published studies have demonstrated the change in Escherichia coli urinary isolate antimicrobial susceptibility over time within a given area and (or) population. The purpose of this study was to evaluate the change in susceptibility of E. coli clinical isolates obtained from u...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-001
更新日期:2012-03-01 00:00:00
abstract::In Escherichia coli, the N-terminal domain of the essential protein FtsK (FtsKN) is proposed to modulate septum formation through the formation of dynamic and essential protein interactions with both the Z-ring and late-stage division machinery. Using genomic mutagenesis, complementation analysis, and in vitro pull-do...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0493
更新日期:2020-04-01 00:00:00
abstract::A stable enrichment culture converting acetic acid to methane was successfully obtained from a pear waste digester, using a synthetic substrate solution with acetic acid as the main carbon source. This enrichment culture converted up to 10 mmol of acetic acid per litre per day at 35 degrees C and did not use hydrogen ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-194
更新日期:1976-09-01 00:00:00
abstract::The aim of this study was to assess the influence of sodium content on the microbiota on the surface of ready-to-eat (RTE) meat products purchased from the retail market in Canada. Products, including sliced and sausage-type deli meats, were analysed with culture-dependent and culture-independent methods. Bacteria wer...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0630
更新日期:2015-02-01 00:00:00
abstract::Of 33 streptococcal cultures belonging to serological group L, all bound human immunoglobulin (Ig) G, fibrinogen, and fibronectin; 32 bound bovine IgG; 31 bound alpha 2-macroglobulin; 5 bound albumin; and none bound either haptoglobin or IgA. The binding sites for IgG could be isolated from the L streptococci by tryps...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-001
更新日期:1988-01-01 00:00:00
abstract::The effect of phenolic compounds, ethyl alcohol, and sodium metabisulphite on the lytic activity of virulent bacteriophage PL-1 on a Lactobacillus casei S strain isolated from a lactic acid beverage fermentation was investigated. Catechin, caffeic, and gallic acids, commercially produced red, white, and champagne tann...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-162
更新日期:1985-09-01 00:00:00