Direct lymphocytotoxicity against herpes simplex virus infected cells.


:This study was undertaken to determine if direct cytotoxicity (DC) against herpes simplex virus infected cells, perhaps mediated by T cells, could be demonstrated in individuals subject to recurrent herpes labialis. The mononuclear cells from 7 out of 17 individuals with recurrent herpes expressed DC whereas no DC was ever exhibited by 7 individuals without a previous history of herpes infections. Several approaches were used to show that the cytotoxicity being detected was predominately of the direct type rather than antibody-dependent cell cytotoxicity (ADCC). Since the effector cells of the DC were sensitive to trypsin treatment and behaved as do natural killer (NK) cells upon cell fractionation, the results were taken to imply that the DC was attributable to a NK-effector cell type rather than a classical T lymphocyte.


Can J Microbiol


Fujimiya Y,Babiuk LA,Rouse BT




Has Abstract


1978-09-01 00:00:00












  • Intrafungal distribution of aflatoxins among conidia and sclerotia of Aspergillus flavus and Aspergillus parasiticus.

    abstract::This research examines the distribution of aflatoxins among conidia and sclerotia of toxigenic strains of Aspergillus flavus Link and Aspergillus parasiticus Speare cultured on Czapek agar (21 days, 28 degrees C). Total aflatoxin levels in conidia and sclerotia varied considerably both within (intrafungal) and among s...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Wicklow DT,Shotwell OL

    更新日期:1983-01-01 00:00:00

  • Characterization of high density monolayers of the biofilm bacterium Caulobacter crescentus: evaluating prospects for developing immobilized cell bioreactors.

    abstract::Caulobacters are biofilm-forming members of the natural flora of soil and aquatic environments, which exhibit several characteristics that make them attractive for development of high surface area microbial bioreactors or biosensors. Although caulobacters are well characterized genetically, little is known about their...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Smit J,Sherwood CS,Turner RF

    更新日期:2000-04-01 00:00:00

  • Rv3351c, a Mycobacterium tuberculosis gene that affects bacterial growth and alveolar epithelial cell viability.

    abstract::Despite the interactions known to occur between various lower respiratory tract pathogens and alveolar epithelial cells (AECs), few reports examine factors influencing the interplay between Mycobacterium tuberculosis bacilli and AECs during infection. Importantly, in vitro studies have demonstrated that the M. tubercu...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Pavlicek RL,Fine-Coulson K,Gupta T,Quinn FD,Posey JE,Willby M,Castro-Garza J,Karls RK

    更新日期:2015-12-01 00:00:00

  • Intrinsic limitations on the continued production of the antibiotic patulin by Penicillium urticae.

    abstract::The initial cause of the cessation of patulin biosynthesis in submerged cultures of Penicillium urticae was sought. The extrinsic limitations to patulin biosynthesis in fermentor cultures were successfully removed and kinetic profiles of the metabolites of the pathway and of three pathway enzymes were determined over ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Neway J,Gaucher GM

    更新日期:1981-02-01 00:00:00

  • Bactericidal activity of magainin 2: use of lipopolysaccharide mutants.

    abstract::Salmonella typhimurium and a series of rough lipopolysaccharide mutants derived from it were used as target bacteria to examine the antimicrobial capacity of magainin 2. Magainin 2 demonstrated a dose-related bactericidal activity against the smooth parent strain and the series of lipopolysaccharide mutants. The lipop...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Macias EA,Rana F,Blazyk J,Modrzakowski MC

    更新日期:1990-08-01 00:00:00

  • Analysis of biofilm formation by intestinal lactobacilli.

    abstract::In this study, the biofilm-forming potential of intestinal Lactobacillus reuteri strains under different culture conditions was characterized by microtiter plate biofilm assays. Moreover, the spatial organization of exogenously applied L. reuteri L2/6 (a pig isolate) at specific locations in gastrointestinal tract of ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Slížová M,Nemcová R,Mad'ar M,Hadryová J,Gancarčíková S,Popper M,Pistl J

    更新日期:2015-06-01 00:00:00

  • A highly efficient second-step concentration technique for bacteriophages and enteric viruses using ammonium sulfate and Tween 80.

    abstract::Addition of Tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. When reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. This floating layer can be obta...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Armon R,Arella M,Payment P

    更新日期:1988-05-01 00:00:00

  • Host cell invasion and intracellular residence by Aeromonas salmonicida: role of the S-layer.

    abstract::Virulent strains of the fish pathogen Aeromonas salmonicida, which have surface S-layers (S+), efficiently adhere to, enter, and survive within macrophages. Here we report that S+ bacteria were 10- to 20-fold more adherent to non-phagocytic fish cell lines than S-layer-negative (S-) mutants. When reconstituted with ex...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Garduño RA,Moore AR,Olivier G,Lizama AL,Garduño E,Kay WW

    更新日期:2000-07-01 00:00:00

  • Impact of glycerol-3-phosphate dehydrogenase on virulence factor production by Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa establishes life-long chronic infections in the cystic fibrosis (CF) lung by utilizing various adaptation strategies. Some of these strategies include altering metabolic pathways to utilize readily available nutrients present in the host environment. The airway sputum contains various host-deriv...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Daniels JB,Scoffield J,Woolnough JL,Silo-Suh L

    更新日期:2014-12-01 00:00:00

  • Comparison of methods for the concentration of Cryptosporidium oocysts and Giardia cysts from raw waters.

    abstract::This study compared the recovery of Cryptosporidium oocysts and Giardia cysts ((oo)cysts) from raw waters using 4 different concentration-elution methods: flatbed membranes, FiltaMax foam, Envirochek HV capsules, and Hemoflow ultrafilters. The recovery efficiency of the combined immunomagnetic separation and staining ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Ferguson C,Kaucner C,Krogh M,Deere D,Warnecke M

    更新日期:2004-09-01 00:00:00

  • Inhibition of Fusarium moniliforme var. subglutinans, the causal agent of pine pitch canker, by the soil bacterium Arthrobacter sp.

    abstract::A species of Arthrobacter was recovered during culture of the causal organism of pitch canker of southern pines. Fusarium moniliforme var. subglutinans (FMS). Arthrobacter is a relatively common soil bacterium and is lytic to several fungal pathogens in the soil. Soil samples from two seed orchards with pitch canker a...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Barrows-Broaddus J,Kerr TJ

    更新日期:1981-01-01 00:00:00

  • Identification of fecal contamination sources in water using host-associated markers.

    abstract::In British Columbia, Canada, drinking water is tested for total coliforms and Escherichia coli, but there is currently no routine follow-up testing to investigate fecal contamination sources in samples that test positive for indicator bacteria. Reliable microbial source tracking (MST) tools to rapidly test water sampl...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Krentz CA,Prystajecky N,Isaac-Renton J

    更新日期:2013-03-01 00:00:00

  • Improving the culturability of freshwater bacteria using FW70, a low-nutrient solid medium amended with sodium pyruvate.

    abstract::Bacterial culture based on the use of plate media is an effective method for investigating bacterial populations in the environment. To improve the culturability of bacteria from freshwater lakes, we developed a new medium, FW70, which contains sodium pyruvate, casamino acids, and lake water and is solidified using ge...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Imazaki I,Kobori Y

    更新日期:2010-04-01 00:00:00

  • Light microscopy observations of tetrazolium-reducing bacteria in the endorhizosphere of maize and other grasses in Brazil.

    abstract::Roots of field-grown tropical maize, Panicum maximum Jacq. and Digitaria decumbens Stent., and of sorghum and wheat grown in monoxenic culture with the diazotroph Spirillum lipoferum (syn. Azospirillum spp.) were examined for tetrazolium-reducing bacteria following incubation of roots in a malate-phosphate buffer-2,3,...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Patriquin DG,Döbereiner J

    更新日期:1978-06-01 00:00:00

  • Effect of toluene on Pseudomonas stutzeri ST-9 morphology - plasmolysis, cell size, and formation of outer membrane vesicles.

    abstract::Isolated toluene-degrading Pseudomonas stutzeri ST-9 bacteria were grown in a minimal medium containing toluene (100 mg·L(-1)) (MMT) or glucose (MMG) as the sole carbon source, with specific growth rates of 0.019 h(-1) and 0.042 h(-1), respectively. Scanning (SEM) as well as transmission (TEM) electron microscope anal...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Michael E,Nitzan Y,Langzam Y,Luboshits G,Cahan R

    更新日期:2016-08-01 00:00:00

  • Exposure of Agrobacterium tumefaciens to agroinfiltration medium demonstrates cellular remodelling and may promote enhanced adaptability for molecular pharming.

    abstract::Agroinfiltration is used to treat plants with modified strains of Agrobacterium tumefaciens for the purpose of transient in planta expression of genes transferred from the bacterium. These genes encode valuable recombinant proteins for therapeutic or industrial applications. Treatment of large quantities of plants for...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Prudhomme N,Pastora R,Muselius B,McLean MD,Cossar D,Geddes-McAlister J

    更新日期:2021-01-01 00:00:00

  • Characterization of 4 T1-like lytic bacteriophages that lyse Shiga-toxin Escherichia coli O157:H7.

    abstract::Bacteriophages are associated with reduced fecal shedding of Shiga-toxin-producing Escherichia coli O157:H7 (STEC O157:H7) in cattle. Four phages exhibiting activity against 12 of 14 STEC O157:H7 strains, representing 11 common phage types, were isolated. Phages did not lyse non-O157 E. coli, with 11 of the 12 STEC st...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Niu YD,Stanford K,Ackermann HW,McAllister TA

    更新日期:2012-07-01 00:00:00

  • Characterization of intronic structures and alternative splicing in Phytophthora sojae by comparative analysis of expressed sequence tags and genomic sequences.

    abstract::The oomycetes, a distinct phylogenetic lineage of fungus-like microorganisms, are heterokonts (stramenopiles) belonging to the supergroup Chromalveolata. Although the complete genomic sequences of a number of oomycetes have been reported, little information regarding the introns therein is available. Here, we investig...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Shen D,Ye W,Dong S,Wang Y,Dou D

    更新日期:2011-02-01 00:00:00

  • Biodegradation of Maya crude oil fractions by bacterial strains and a defined mixed culture isolated from Cyperus laxus rhizosphere soil in a contaminated site.

    abstract::Ten bacterial strains were isolated by enrichment culture, using as carbon sources either aliphatics or an aromatic-polar mixture. Oxygen uptake rate was used as a criterion to determine culture transfer timing at each enrichment stage. Biodegradation of aliphatics (10,000 mg L(-1)) and an aromatic-polar mixture (5000...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Díaz-Ramírez IJ,Ramírez-Saad H,Gutiérrez-Rojas M,Favela-Torres E

    更新日期:2003-12-01 00:00:00

  • Interactions of Phytophthora cinnamomi and Trichoderma spp. in relation to propagule production in soil cultures at 26 degrees C1.

    abstract::Effects of Trichoderma harzianum and T. polysporum on chlamydospore production by two isolates of Phytophthora cinamomi were determined over a 21-day period in nonsterile, sterilized, and sterilized amended soil. Trichoderma was either coinoculated with P. cinnamomi or added to the cultures on day 3 of the incubation ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Kelley WD

    更新日期:1977-03-01 00:00:00

  • Amino acid uptake systems in Bacteroides ruminicola.

    abstract::Uptake of amino acids by Bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. A high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. All amino acids except proline we...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Stevenson RM

    更新日期:1979-10-01 00:00:00

  • Effect of ultraviolet-induced mutants of Trichoderma harzianum with altered antibiotic production on selected pathogens in vitro.

    abstract::Mutants of Trichoderma harzianum with altered antibiotic production were isolated using ultraviolet light mutagenesis. These included strains whose activity in a Fusarium oxysporum spore germination assay was greater than twice that of the parental strain and one that had no detectable antifungal activity. Characteris...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Graeme-Cook KA,Faull JL

    更新日期:1991-09-01 00:00:00

  • Molecular techniques and their potential application in monitoring the microbiological quality of indoor air.

    abstract::Health effects associated with poor indoor air quality have created a need for accurate, reproducible methods of monitoring the microbiological content of indoor air. Improved methods of detection may allow researchers to clarify the effect of individual species present in the indoor environment on human health. This ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章,评审


    authors: MacNeil L,Kauri T,Robertson W

    更新日期:1995-08-01 00:00:00

  • Menaquinone reduction by an HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus.

    abstract::The gene-encoding HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus JCM2501 was amplified and expressed in Escherichia coli and the enzymatic features were examined. The enzyme was detected mainly in the membrane fraction. It catalyzed the sulfide-dependent menaquinone (MK) reduction showing special enz...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Shibata H,Suzuki K,Kobayashi S

    更新日期:2007-09-01 00:00:00

  • Acetylation and nitrosation of ciprofloxacin by environmental strains of mycobacteria.

    abstract::To determine the ability of environmental bacteria to metabolize the frequently prescribed fluoroquinolone drug ciprofloxacin, eight Mycobacterium spp. cultures were grown for 4 days in a medium containing sorbitol and yeast extract with 100 mg x L(-1) ciprofloxacin. After the cultures had been centrifuged and the sup...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Adjei MD,Heinze TM,Deck J,Freeman JP,Williams AJ,Sutherland JB

    更新日期:2007-01-01 00:00:00

  • Cell-wall-bound metal ions are not taken up in Neurospora crassa.

    abstract::To establish the relevance of the cell wall in metal ion transport, cobalt uptake was examined in Neurospora crassa. Cobalt taken up was largely surface bound (>90%), resulting in a release of calcium and magnesium. Surface-bound cobalt could not enter intracellular locations upon further incubation of mycelia in a me...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Latha JN,Rashmi K,Mohan PM

    更新日期:2005-12-01 00:00:00

  • The biodegradation of methoxychlor by Klebsiella pneumoniae.

    abstract::Klebsiella pneumoniae was found to effect the reductive dechlorination of methoxychlor (2,2-bis(p-methoxyphenyl)-1, 1, 1-trichloroethane) and its dihydroxy derivative (2, 2-bis (p-hydroxyphenyl)-1, 1, 1-trichloroethane) to dichloro derivatives 2, 2-bis (p-methoxyphenyl)-1, 1-dichloroethane and 2, 2-bis (p-hydroxypheny...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Baarschers WH,Bharath AI,Elvish J,Davies M

    更新日期:1982-02-01 00:00:00

  • Dietary fructooligosaccharides alter the cultivable faecal population of rats but do not stimulate the growth of intestinal bifidobacteria.

    abstract::The effect of fructans on the cultivable faecal community of Bio Breeding rats fed diets containing 5% (m/v) food-grade fructooligosaccharide (FOS) was investigated. Culturing of faecal material using chicory inulin as the sole carbohydrate source revealed the presence of a greater diversity of inulin-utilizing bacter...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Gourgue-Jeannot C,Kalmokoff ML,Kheradpir E,Kwan J,Lampi BJ,McAllister M,Brooks SP

    更新日期:2006-10-01 00:00:00

  • Detection and characterization of bacteria from the potato rhizosphere degrading N-acyl-homoserine lactone.

    abstract::Quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. The signal molecules involved in quorum sensing in P. carotovorum subsp. carotovorum belong to the group of N-acyl homoserine lactones (AHLs). In our study, we scre...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Jafra S,Przysowa J,Czajkowski R,Michta A,Garbeva P,van der Wolf JM

    更新日期:2006-10-01 00:00:00

  • Leakage of periplasmic enzymes from lipopolysaccharide-defective mutants of Salmonella typhimurium.

    abstract::Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC, cyclic phosphodiesterase, ribonuclease I (EC, and phosphoglucose isomerase (EC (PGI is at least par...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章


    authors: Chatterjee AK,Ross H,Sanderson KE

    更新日期:1976-10-01 00:00:00