Abstract:
:We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in a dctA gene. Levels of dctA gene expression were determined under different environmental conditions using dctA-lacZ fusions, and beta-galactosidase activities increased in response to osmotic stress and also when the cells were incubated in medium low in calcium. The transcriptional induction of the dctA gene by environmental signals was decreased by DNA gyrase inhibitors such as novobiocin and coumermycin.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Batista S,Castro S,Aguilar OM,Martínez-Drets Gdoi
10.1139/m92-008subject
Has Abstractpub_date
1992-01-01 00:00:00pages
51-5issue
1eissn
0008-4166issn
1480-3275journal_volume
38pub_type
杂志文章abstract::The mouse model of intraperitoneal sepsis with Proteus rettgeri was used to evaluate the anti-endotoxic effect of polymyxin B sulfate. An unexpected reversal of the usual protective effect of polymyxin in experimental enterobacterial sepsis was observed in which the lethality of the infection was enhanced. ...
journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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