Abstract:
:Plant-microbial interactions have been well studied because of the ecological importance of such relationships in aquatic systems. However, general knowledge regarding the composition of these biofilm communities is still evolving, partly as a result of several confounding factors that are attributable to plant host properties and to hydrodynamic conditions in aquatic environments. In this study, the occurrences of various bacterial phylogenetic taxa on 2 native plants, i.e., mayapple (Podophyllum peltatum L.) and cow parsnip (Heracleum maximum Bartram), and on an invasive species, i.e., garlic mustard (Alliaria petiolata (M. Bieb.) Cavara & Grande), were quantitatively examined using nucleic acid staining and fluorescence in situ hybridization. The plants were incubated in triplicates for about a week within the Kalamazoo River and Pierce Cedar Creek as well as in microcosms. The bacterial groups targeted for enumeration are known to globally occur in relatively high abundance and are also ubiquitously distributed in freshwater environments. Fluorescence in situ hybridization analyses of the bacterioplankton assemblages revealed that the majority of bacterial cells that hybridized with the different probes were similar between the 2 sites. In contrast, the plant-associated populations while similar on the 3 plants incubated in Kalamazoo River, their representations were highest on the 2 native plants relative to the invasive species in Pierce Cedar Creek. Overall, our results further suggested that epiphytic bacterial assemblages are probably under the influences of and probably subsequently respond to multiple variables and conditions in aquatic milieus.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Olapade OA,Pung Kdoi
10.1139/w2012-053subject
Has Abstractpub_date
2012-06-01 00:00:00pages
767-75issue
6eissn
0008-4166issn
1480-3275journal_volume
58pub_type
杂志文章abstract::In both relA+ and relA- derivatives, the biosynthesis of peptidoglycan, lipid intermediates, and nucleotide precursors abruptly halted at the onset of diauxic lag from glucose to lactose with a concomitant accumulation of guanosine 5'-diphosphate 3'-diphosphate (ppGpp). These results are consistent with the proposal t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-188
更新日期:1979-10-01 00:00:00
abstract::Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-015
更新日期:1989-01-01 00:00:00
abstract::The expression of the toxA and regA genes in Pseudomonas aeruginosa is negatively regulated by iron at the transcriptional level. We have previously described ptxR, an exotoxin A regulatory gene which appears to enhance toxA expression through regA. In this study, we have tried to determine if ptxR expression correlat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Increasing the use of nitrogen fertilizers in tea orchards has led to intense nitrous oxide (N2O) emissions. Foliar application of Paenibacillus polymyxa biofertilizer has been proven to be beneficial for organic tea production. In this study, tea yield and quality were significantly improved after application of P. p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0511
更新日期:2020-03-01 00:00:00
abstract::In the dairy industry, bacterial contaminants persist on equipment surfaces due to spore and biofilm formation. These are involved in cross-contamination problems that affect the quality of processed products and limit their life. The pasteurization process, in which milk is submitted to moderate heat treatment, is in...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/cjm-2018-0435
更新日期:2019-06-01 00:00:00
abstract::In Neurospora crassa, heat shock results in the induction of 9 to 11 heat shock proteins (HSP), of which HSP80 is the most abundant and the first to be synthesized. The induction of HSP80 was investigated during normal growth (2% sucrose) and under sucrose starvation. Transfer of mycelium to a medium supplemented with...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-031
更新日期:1988-02-01 00:00:00
abstract::The physiological characteristics and fatty acid composition of 40 strains of Yarrowia lipolytica isolated from irradiated poultry meat, commercial chilled foods, the superficial water of lagoons of an Italian river delta, and commercial light butter were compared. Discriminant analysis of the results permitted the 40...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-008
更新日期:1994-01-01 00:00:00
abstract::To assess the effect of fire and salvage logging on the diversity of mycorrhizal-bacterial communities, bacteria associated with Cenococcum, Thelephora, Tomentella, Russulaceae, and E-strain ectomycorrhizae (ECM) of Abies lasiocarpa seedlings were characterized using two approaches. First, bacteria were isolated and c...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-056
更新日期:2002-07-01 00:00:00
abstract::The susceptibility of congenitally immunodeficient mice to a nonencapsulated strain of Cryptococcus neoformans (strain M7) was evaluated. Gnotobiotic mice with defined congenital defects in innate immunity (beige) or cell-mediated immunity (athymic) or with combined defects in innate and cellular immunity (beige athym...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-144
更新日期:1991-11-01 00:00:00
abstract::Antibiotic susceptibility tests and restriction enzyme analysis (REA) of genomic DNA were performed to characterize the relationship between sources of isolates of yellow-pigmented enterococci. Antibiotic susceptibility tests were conducted with 10 therapeutic antibiotics and 54 isolates grouped by source (wild and ot...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-44-11-1066
更新日期:1998-11-01 00:00:00
abstract::The antiserum agar method (ASA), which is based on the formation of immunoprecipitates around bacterial growth on agar containing meningococcal hyperimmune horse serum, was evaluated for serogroup identification of Neisseria meningitidis. Four hundred meningococcal stains were serogrouped by ASA employing horse antise...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-113
更新日期:1979-06-01 00:00:00
abstract::Hydrogen cyanide production by Pseudomonas aeruginosa growing in a synthetic medium required aerobosis but operated efficiently at low dissolved oxygen concentration. Half maximum levels of cyanogenesis occurred at 0.015 microM oxygen; maximum cyanogenesis occurred over a wide range, 0.1-180 microM, of oxygen concentr...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-209
更新日期:1983-10-01 00:00:00
abstract::The use of the food-grade bacterium Lactococcus lactis as a new cell factory is a promising alternative expression system for producing a desired protein. The Omp16-IL2 fusion protein antigen was cloned, expressed, and purified in this study. The Omp16-IL2 fusion gene was designed and cloned in pGH plasmid with approp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0261
更新日期:2020-01-01 00:00:00
abstract::The metabolism of cyclohexanecarboxylic acid by a bacterium, designated PRL W19, follows a pathway involving beta-oxidation of coenzyme A intermediates analogous to the classical oxidation of fatty acids. The organism appears to have the property for the constitutive metabolism of caproic acid, and cell extracts conta...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-141
更新日期:1978-07-01 00:00:00
abstract::Thirty Haemophilus strains and six Actinobacillus strains, all of porcine origin, were examined for their biochemical reactivity on API 20E and API ZYM test strips using dense cell suspensions (supplemented with NAD as appropriate) as strip inocula. When combined with a test for V-factor dependency, the use of both st...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-195
更新日期:1984-10-01 00:00:00
abstract::A strain of Pseudomonas putida grew rapidly on cyclohexanecarboxylic acid as a sole source of carbon. A CoA-mediated beta-oxidation pathway was induced for the metabolism of the compound. The organism could not utilize 3-cyclohexenecarboxylic acid as a sole source of carbon for growth, but cells grown on gluconate in ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-198
更新日期:1982-12-01 00:00:00
abstract::A commercially available single-bottle blood culture system was evaluated at Ben Taub General Hospital, a Harris County District Hospital. Blood cultures from 1010 patients were examined with the Lederle Diagnostics one-bottle blood culture medium-SPS, Columbia broth (E-Vac, Pfizer), and an in-house-prepared brain hea...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-155
更新日期:1977-08-01 00:00:00
abstract::The induction of antituberculous immunity by crude ribosomal fractions isolated from Mycobacterium tuberculosis strain H37Ra, M. bovis strain BCG, and M. smegmatis was studied in CF-1 mice. Levels of antituberculous immunity similar to that induced by live BCG were induced by the BCG and H37Ra ribosomal fractions wher...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-032
更新日期:1983-02-01 00:00:00
abstract::The Bacillus subtilis glutamyl-tRNA synthetase (GluRS), encoded by the gltX gene, aminoacylates its homologous tRNA(Glu) and tRNA(Gln) with glutamate. This gene was cloned with its sigma A promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and B. s...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::The effects of various hydrocarbon substrates, and a chemical surfactant capable of enhancing crude-oil biodegradation, on the community structure of a mixed-bacterial inoculum were examined in batch culture. Of 1000 TSA-culturable isolates, 68.6% were identified at the genus level or better by phospholipid fatty acid...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::The sensitivity of delta red-gam delta ren mutants of bacteriophage lambda to Rex exclusion by lambda rexA+ rexB+ lysogens is modulated by the prophage cI repressor allele. We show the following: (i) lambda spi156 delta nin5 forms plaques on a cI+-rexA+-rexB+ lysogen with 10(5)-fold higher efficiency than on cI[Ts]-re...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-021
更新日期:2003-03-01 00:00:00
abstract::Fluorescein isothiocyanate-conjugated antibody to purified enterotoxin of Clostridium perfringens was used to study the intracellular formation of enterotoxin by this organism. Enterotoxin was detected at 4 h of growth at the end of the cell containing forespore. With the development of the spore, enterotoxin accumula...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-134
更新日期:1977-07-01 00:00:00
abstract::Using a gene fusion approach, hybrid proteins were created by linking alkaline phosphatase (PhoA) or a cellulase reporter (delta CenA) to four large N-terminal portions of the Caulobacter crescentus surface (S)-layer protein (RsaA; 1026 amino acids). Three of the sites (amino acids 189, 220, 315) were selected on the ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-122
更新日期:1994-09-01 00:00:00
abstract::The ultraviolet germicidal irradiation (UVGI) dose necessary to inactivate fungal spores on an agar surface and the efficacy of UVGI were determined for cultures of Stachybotrys chartarum (ATCC 208877). This study employed a UVGI testing unit consisting of four chambers with a 9-W, Phillips, low pressure, mercury UVGI...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-061
更新日期:2005-09-01 00:00:00
abstract::A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-106
更新日期:1983-06-01 00:00:00
abstract::For the first time, an Escherichia coli strain producing four microcins (Mcc), B17, D93, J25, and L, and showing immunity to Mcc V was isolated and characterized. Each of the gene clusters encoding the production of Mcc B17, D93, and L was cloned separately. The gene cluster for Mcc L was cloned within a 13.5-kb HindI...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-047
更新日期:2003-05-01 00:00:00
abstract::No clear data are available on how flavonoids from different chemical groups affect root colonization by arbuscular mycorrhizal fungi (AMF) and whether flavonoids affecting the presymbiotic growth of AMF also affect root colonization by AMF. In the present work, we compared the effect of flavones (chrysin and luteolin...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-036
更新日期:2007-06-01 00:00:00
abstract::Wild-type glucose uptake and glucose chemotaxis activities were restored in glucose transport defective Pseudomonas aeruginosa strains PFB360 and PFB362 after introduction of plasmid pPZ129, containing a 1.1-kilobase DNA fragment that is essential for the expression of the P. aeruginosa periplasmic glucose binding pro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-163
更新日期:1993-11-01 00:00:00
abstract::During growth on minimal salts - glucose media supplemented with high concentrations of Na2SO3 (10-3 and 10-2 M), Salmonella heidelberg exhibited cytological and growth responses which indicated increased cellular toxicity with increasing sulfite concentrations. The large quantities of sulfide evolved during growth at...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-217
更新日期:1979-12-01 00:00:00
abstract::The monitoring of antimicrobial susceptibilities in Neisseria gonorrhoeae isolates and characterization of N. gonorrhoeae multiantigen sequence types (NG-MAST, ST) provide important surveillance data as resistance rates continue to rise. A total of 2970 N. gonorrhoeae isolates were collected by Canadian provincial pub...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0357
更新日期:2013-10-01 00:00:00