Abstract:
:The antiserum agar method (ASA), which is based on the formation of immunoprecipitates around bacterial growth on agar containing meningococcal hyperimmune horse serum, was evaluated for serogroup identification of Neisseria meningitidis. Four hundred meningococcal stains were serogrouped by ASA employing horse antisera to serogroups A, B, C, Y, W135, Z, and 29E and compared to serogroup identification by bacterial slide agglutination (BA) employing rabbit antisera. Overall, there was 95% agreement between the two methods. The ASA proved to be more accurate than BA since 15 strains which cross-reacted with Y and W135 rabbit antisera by BA were specifically serogrouped as either Y or W135 by ASA. In addition, 5 out of 75 strains which were ungroupable by BA were serogrouped as either B or 29E by ASA. Repeat serogroup identification of 100 meningococcal strains by ASA provided identical results thus showing the reproducibility of the method. The ASA is advantageous to BA since it is more reliable, utilizes standard antisera which do not have to be absorbed to remove cross-reactions, does not require the preparation of standardized bacterial antigen, and is simple to perform.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Ashton FE,Ryan A,Diena BB,Frasch CEdoi
10.1139/m79-113subject
Has Abstractpub_date
1979-06-01 00:00:00pages
784-7issue
6eissn
0008-4166issn
1480-3275journal_volume
25pub_type
杂志文章abstract::In a previous study it was shown that the number of wood-inhabiting bacteria was drastically reduced after colonization of beech (Fagus sylvatica) wood blocks by the white-rot fungus Hypholoma fasciculare, or sulfur tuft (Folman et al. 2008). Here we report on the mechanisms of this fungal-induced antibacterial activi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-023
更新日期:2010-05-01 00:00:00
abstract::Two rod-shaped viruses of similar length occurring in hops were isolated from a mixed infection. One (hop mosaic virus) infected Nicotiana clevelandii, produced mosaic symptoms in mosaic-sensitive hop cultivars, and did not infect Chenopodium murale. The other (hop latent virus) infected C. murale but not N. cleveland...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-168
更新日期:1976-08-01 00:00:00
abstract::Eighteen yeast species of the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus, each one represented by its type strain, were investigated with the objective of evaluating their carotenoid composition. The pigments were extracted from yeast cells, quantified by high pressure liquid chromatography ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-068
更新日期:2007-08-01 00:00:00
abstract::Pathogenic and nonpathogenic isolates of Venturia inaequalis were grown in liquid culture. Hyphae were treated with two types of fimbrial antiserum (AU- and AV-1) and examined by immunofluorescent microscopy, in order to establish the distribution of fimbrial epitopes in whole cell mounts. The AV-1 antiserum was speci...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-061
更新日期:2000-09-01 00:00:00
abstract::Nitrogen-fixing, field-obtained root nodules of the silky green alder were studied by transmission electron microscopy. The nodule endophyte exhibited a prokaryotic cytology and was present in two forms: the hypha(0.3-1.0mum), which was branched and septate, and the vesicle (3-5mum), which was also septate and develo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-157
更新日期:1975-07-01 00:00:00
abstract::Soil bacteria that utilize inositol hexaphosphate (IHP) were isolated from a range of soils using defined selection media. An analysis of 200 randomly selected isolates indicated that less than 0.5% of the culturable population of soil bacteria were capable of using IHP as a sole source of C and P. From a further 238 ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m97-073
更新日期:1997-06-01 00:00:00
abstract::Iron and sulfur oxidation by Thiobacillus ferrooxidans as well as growth on ferrous iron were inhibited by a variety of low molecular weight organic compounds. The influences of chemical structure of the organic inhibitors, pH, temperature, physical treatment of cells, and added inhibitory or stimulatory inorganic ion...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-105
更新日期:1976-05-01 00:00:00
abstract::The regulation of de novo pyrimidine biosynthesis in Pseudomonas putida ATCC 17536 by pyrimidines was explored. The pathway enzyme activities were higher in glucose-grown cells than in succinate-grown cells, indicating catabolite repression by succinate. In P. putida cells grown on succinate as a carbon source, only a...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-110
更新日期:2002-12-01 00:00:00
abstract::Environmental scanning electron microscopic observations of Postia placenta grown on a defined medium and on red spruce wood allowed for the examination of the hydrated sheath of P. placenta. In the wood environment, mature hyphae that were not adhering to the substrate were observed to have a mycofibrillar morphology...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-058
更新日期:1995-04-01 00:00:00
abstract::The fatty acid pattern in Mycobacterium vaccae strain JOB5 was examined after growth on n-alkanes (C14-C18), 1-alkenes (C14-C18), 2- or 3-methyl octadecane, and 8-heptadecene. It was evident that monoterminal oxidation of n-alkanes was followed by beta-oxidation and that both parent fatty acid and products of beta-oxi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-012
更新日期:1975-01-01 00:00:00
abstract::The respiration of Candida lipolytica on n-tetradecane is decreased in the presence of bentonite. This inhibition is less pronounced by the introduction of hydrophobic chains on the bentonite. Oxygen demand of resting cells varies with the length of hydrophobic chains. In fermentor, addition of adsorbant does not stim...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract::The purpose of this study was to examine antimicrobial activity of endophytic fungi isolated from the leaves, stems, and roots of 54 species of Orchidaceae collected in a Brazilian tropical ecosystem. In total, 382 filamentous fungi and 13 yeast isolates were obtained and cultured to examine the production of crude ex...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W09-101
更新日期:2009-12-01 00:00:00
abstract::The repair of DNA damage caused by ultraviolet radiation (UVR) is well understood in both lower and higher organisms. Genetic studies carried out at optimum temperature for growth, 37 °C in Escherichia coli, have revealed the major pathways of DNA repair. We show that E. coli cells grown at 20 °C are more sensitive to...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0874
更新日期:2014-05-01 00:00:00
abstract::Ten bacteriophages infecting Micrococcus luteus have been characterized. All phages contain double-stranded DNA, of 64.3--73.5 mol% guanine plus cytosine (GC). The DNA of phage N7 has the highest GC content reported for any bacterial virus. No unusual bases have been found. The intracellular replicating DNAs of six ph...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-158
更新日期:1979-09-01 00:00:00
abstract::Hydrogen cyanide production by Pseudomonas aeruginosa growing in a synthetic medium required aerobosis but operated efficiently at low dissolved oxygen concentration. Half maximum levels of cyanogenesis occurred at 0.015 microM oxygen; maximum cyanogenesis occurred over a wide range, 0.1-180 microM, of oxygen concentr...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-209
更新日期:1983-10-01 00:00:00
abstract::Separation of Vero cell membrane in a discontinuous sucrose gradient reveals five fractions. After infection a sixth fraction appears. It contains virioins but mostly modified membranes with subunits 5-6 nm in diameter, probably the hemagglutinin. None of the enzymes used was associated with this fraction. No modifica...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-103
更新日期:1975-05-01 00:00:00
abstract::Microbial mats that develop in shallow brackish and hyposaline ponds in the rims of two French polynesian atolls (Rangiroa and Tetiaroa) were intensively investigated during the past three years. Comparative assessment of these mats (called kopara in polynesian language) showed remarkable similarities in their composi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
abstract::Cellobiose transport by the cellulolytic ruminal anaerobe Fibrobacter (Bacteroides) succinogenes was measured using randomly tritiated cellobiose. When assayed at the same concentration (1 mM), total cellobiose uptake was one-fourth to one-third that of total glucose uptake. The abilities of F. succinogenes to transpo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-021
更新日期:1991-02-01 00:00:00
abstract::The diversity of 16 strains of chickpea-infective rhizobia from various geographical origins was analysed using genotypic and phenotypic approaches. Multilocus enzyme electrophoresis was performed, and restriction fragment length polymorphisms of the amplified 16S + IGS (intergenic spacer) rRNA gene, assimilation of 1...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-057
更新日期:1994-05-01 00:00:00
abstract::Pestalotiopsis species were most dominant endophytic species isolated from four medicinal plants including Terminalia arjuna, Terminalia chebula, Azadirachta indica, and Holarrhena antidysenterica. Thirty Pestalotiopsis species isolated from different parts of the medicinal plants were selected for the study. The anti...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-070
更新日期:2008-09-01 00:00:00
abstract::Sixty-four species of filamentous fungi from five flare pits in northern and western Canada were tested for their ability to degrade crude oil using gas chromatographic analysis of residual hydrocarbons following incubation. Nine isolates were tested further using radiorespirometry to determine the extent of mineraliz...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Three composts were tested for their ability to suppress root and stem rot caused by the soil borne fungal pathogen Fusarium oxysporum f. sp. radicis-cucumerinum (FORC) on cucumber. Two of the composts were prepared from separated dairy solids either by windrow (WDS) or vermicomposting (VMC) while the third, obtained ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-082
更新日期:2000-11-01 00:00:00
abstract::The aim of this study was to unravel, by focusing on cell surface properties, the underlying virulence factors contributing to the difference in the pathogenicity observed in two Acinetobacter baumannii strains isolated from the same patient. The two strains were phenotypically different: (i) a mucoid strain (AB-M),...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-131
更新日期:2012-03-01 00:00:00
abstract::The activities of isocitrate lyase, esterase, and lipase by the psychrotrophic Acinetobacter sp. strain HH1-1 were monitored during incubation at 25 degreesC, 5 degreesC, and after a 25 degreesC to 5 degreesC down shift in growth temperature. During growth at 25 degreesC, isocitrate lyase activity was detected in cell...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::DNA-degrading activity from anaerobic samples of bovine ruminal fluid, primary anaerobic digestor wastewater, freshwater sediments, and marine sediments was observed in the presence of 5 mM EDTA. Nuclease activity experiments involved exposing salmon chromosomal DNA to the environmental samples in 50 mM pH 7.2 buffer,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::Glutaraldehyde, the biological fixative of choice in the cytochemical localization of the phosphatases, was investigated for its effects on Pseudomonas aeruginosa alkaline phosphatase. Comparative studies on the inactivation of alkaline phosphatase by glutaraldehyde showed significant differences when the purified pro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-078
更新日期:1981-05-01 00:00:00
abstract::Agricultural practices such as manure applications could contribute to the spread of antibiotic resistance genes (ARGs) within the environment. Our objective was to assess the impact of certain fertilization methods (mineral or manure) and tillage practices (reduced or conventional) on the presence of ARGs and bacteri...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0343
更新日期:2020-10-01 00:00:00
abstract::The induction of nitrogenase (C2H2) activity in asymbiotically cultured Rhizobium sp. 32H1 was found to be associated with morphological changes in the cells which were more pronounced than those seen in bacteroids. Polyphosphate granules were found in both bacteroids and cultured cells, but poly-beta-hydroxybutyrate ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-055
更新日期:1979-03-01 00:00:00
abstract::Canadian oil sands tailings are predominately sodic residues contaminated by hydrocarbons such as naphthenic acids. These conditions are harsh for plant development. In this study, we evaluated the effect of inoculating roots of Alnus viridis ssp. crispa and Alnus incana ssp. rugosa with ectomycorrhizal fungi in the p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0703
更新日期:2016-07-01 00:00:00
abstract::Like recF and recQ mutants studied earlier, two other classes of Escherichia coli mutants defective in the RecF conjugal recombination pathway, recJ and recO, were found to be partially resistant to thymineless death. In contrast, a recN mutant, also belonging to the pathway, was indistinguishable from the wild type w...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-157
更新日期:1988-07-01 00:00:00