Abstract：
:The activities of isocitrate lyase, esterase, and lipase by the psychrotrophic Acinetobacter sp. strain HH1-1 were monitored during incubation at 25 degreesC, 5 degreesC, and after a 25 degreesC to 5 degreesC down shift in growth temperature. During growth at 25 degreesC, isocitrate lyase activity was detected in cell-free extracts, but at 5 degreesC and after cold shock, activity was measured primarily in the cell culture supernatant. Strain HH1-1 produced two cell-associated esterases and an extracellular esterase and lipase. Activities of the extracellular esterase and lipase were reduced when cells were grown at 5 degreesC and after cold shock. In contrast, an increased synthesis of a 53-kDa cell-associated esterase was observed 50 h after cold shock. An extracellular polysaccharide was also produced, indicated by a decrease in surface tension in cell culture supernatant when cells were incubated at 25 degreesC; but like extracellular enzyme activity, production of the exopolymer was reduced when cells were subjected to low temperatures. These results indicated that the intracellular enzyme, isocitrate lyase, leaked out of the cell after cold shock and during growth at 5 degreesC. The increased activity of a cell-associated esterase suggested this enzyme is required for growth at low temperatures. In contrast, activities of extracellular lipolytic enzymes and production of an extracellular polysaccharide were negatively affected at the lower temperatures.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Barbaro SE,Trevors JT,Inniss WEsubject
Has Abstractpub_date
2001-03-01 00:00:00pages
194-205issue
3eissn
0008-4166issn
1480-3275journal_volume
47pub_type
杂志文章abstract：:The acetylene-reduction assay was used for in situ and laboratory assessment of biological nitrogen fixation in the acidic, waterlogged, muskeg ecosystem of the southern James Bay area, in the region of Moosonee, Ontario. In situ assays and subsequent laboratory experimentation revealed that nitrogenase activity was p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-130
更新日期：1976-07-01 00:00:00
abstract：:Studies of the molecular biology of human papillomavirus type 16 have been limited by the lack of a tissue culture system that is fully permissive for virus replication; as a result, high-titre stocks of infectious virus are not readily available. Therefore, studies of viral gene expression have relied on analysis of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-016
更新日期：1993-01-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-083
更新日期：1994-06-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-083
更新日期：2012-09-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-106
更新日期：1983-06-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-190
更新日期：1976-09-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-083
更新日期：2007-10-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-038
更新日期：1981-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-251
更新日期：1975-11-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-031
更新日期：1984-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-036
更新日期：2003-04-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0703
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0708
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-072
更新日期：2012-09-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-257
更新日期：1983-12-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-124
更新日期：1978-06-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-012
更新日期：2006-06-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-158
更新日期：1979-09-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-065
更新日期：1984-04-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-228
更新日期：1977-11-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-44-11-1066
更新日期：1998-11-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-066
更新日期：2008-10-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:A moderately halophilic Acinetobacter sp., capable of producing dextrinogenic amylase, was isolated from sea-sands. Maximum enzyme production was obtained when the bacterium was cultivated aerobically in media containing 1 to 2M NaCl or 1M KCl. Two kinds of amylase, amylases I and II were purified from the culture fil...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-169
更新日期：1978-09-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-049
更新日期：1978-03-01 00:00:00
abstract：:The rumen microbiota plays a major role in the metabolism and absorption of indigestible food sources. Xinjiang brown cattle (Bos taurus), Tarim red deer (Cervus elaphus yarkandensis), and Karakul sheep (Ovis aries) are important ruminant species for animal husbandry in the Tarim Basin. However, the microbiota and rum...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2016-0596
更新日期：2017-05-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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更新日期：2013-08-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-023
更新日期：2010-05-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1976-07-01 00:00:00
abstract：:Penicillium chrysogenum F1 is very efficient in bioleaching heavy metals from the soil and is used for that purpose. We found that F1 can extract 19.8 mg Cd, Cu, Pb, and Zn from 2.5 g soil; the total heavy metals' bioleaching ratio was 60.4%. In this study, the bioleaching mechanism was investigated by means of metabo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2018-0624
更新日期：2019-09-01 00:00:00
