Abstract：
:Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stationary phase of growth is mutated with a frequency of 10(-5). The difference in the mutation susceptibility is due to the dynamics of plasmid pHH1. p-vac gene mutations are caused (i) by the integration of an insertion element or (ii) by a deletion event encompassing the p-vac gene region. In contrast, c-vac mutants analyzed to date incurred neither insertion elements nor deletions. Deletion events within pHH1 occur at high frequencies during the development of a H. halobium culture. The investigation of the fusion regions resulting from deletion events indicates that insertion elements are involved. The analysis of pHH1 deletion variants led to a 4 kilobase pair DNA region containing the origin of replication of the pHH1 plasmid.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Pfeifer F,Blaseio U,Horne Mdoi
10.1139/m89-015subject
Has Abstractpub_date
1989-01-01 00:00:00pages
96-100issue
1eissn
0008-4166issn
1480-3275journal_volume
35pub_type
杂志文章abstract：:Microsclerotia of three melanin-deficient mutants of Verticillium dahliae formed malanin from (+)-scytalone, 1,8-dihydroxynaphthalene, catechol, and L-3,4-dihydroxyphenylalanine. The melanins formed from (+)-scytalone or 1,8-dihydroxynaphthalene resembled wild-type melanin chemically and ultrastructurally, whereas the...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-049
更新日期：1978-03-01 00:00:00
abstract：:A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-106
更新日期：1983-06-01 00:00:00
abstract：:An intracellular invertase was induced in cultures of Clostridium pasteurianum utilizing sucrose as its carbon source for growth. This enzyme synthesis could be repressed by the addition of fructose of a sucrose-growing culture. In contrast, invertase activity was not affected by the addition of glucose to sucrose-gro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-251
更新日期：1975-11-01 00:00:00
abstract：:In Salmonella typhimurium LT2 the F factor of Escherichia coli K-12 replicates normally but is repressed; Flac+ cells give no visible lysis on solid media with male-specific phages, low frequency transfer of Flac+ (0.001-0.007 per donor cell), few f2 infective centers (0.002-0.006 per cell), and they propagate male-sp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-184
更新日期：1983-09-01 00:00:00
abstract：:Probiotics are usually isolated from the gastrointestinal tract of humans and animals. The search of probiotics in human milk is a recent field of research, as the existence of the human milk microbiome was discovered only about a decade ago. To our knowledge, no reports regarding the potential probiotic effect of bac...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0811
更新日期：2016-06-01 00:00:00
abstract：:Ten bacteriophages infecting Micrococcus luteus have been characterized. All phages contain double-stranded DNA, of 64.3--73.5 mol% guanine plus cytosine (GC). The DNA of phage N7 has the highest GC content reported for any bacterial virus. No unusual bases have been found. The intracellular replicating DNAs of six ph...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-158
更新日期：1979-09-01 00:00:00
abstract：:Eighteen gram-negative marine bacteria and two terrestrial species, Escherichia coli and Pseudomonas aeruginosa, were examined for their sensitivity to lysis in distilled water after exposure to a salt solution containing a sea water concentration of Mg2+ (0.05 M) or to 0.5 M NaCl. A spectrum of lytic susceptibility w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-063
更新日期：1982-04-01 00:00:00
abstract：:The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoea colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 microgram of LPS, and challenged intra...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-022
更新日期：1978-02-01 00:00:00
abstract：:The cellular functions are strongly influenced by the composition of the environment. In particular, phenotypes of microbial strains are modulated by concentrations of ions in the culture medium, and differences in element levels may be responsible for a phenotypic variability observed when microbial strains are grown...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-012
更新日期：2007-04-01 00:00:00
abstract：:Isoquinoline is a nitrogen heterocyclic compound that is associated with coal- and oil-derived wastes. Four strains of bacteria able to degrade isoquinoline in pure culture were isolated from sites known to be contaminated with oil. Isoquinoline was used as the sole source of carbon and nitrogen by these isolates. Iso...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-089
更新日期：1994-07-01 00:00:00
abstract：:A nystatin-resistant mutant of Aspergillus niger is described that has quantitative changes in phospholipid composition compared with the wild type. The proportion of linoleic acid in total phospholipids was elevated in the mutant from 38 to 56% and the proportion of oleic acid was lowered from 28 to 12%. Amino acid t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-076
更新日期：1990-06-01 00:00:00
abstract：:A morphological and physiological description of an anamorphic basidiomycetous yeast species, named Trichosporon guehoae (CBS 8521T), is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w99-055
更新日期：1999-08-01 00:00:00
abstract：:Monoclonal antibodies reacting with the B subunit of Vibrio cholerae O1 strain 569B cholera toxin (CT-B) were used to identify unique and common epitopes of V. cholerae non-O1 and Vibrio mimicus CT-B. Vibrio cholerae non-O1 strains produced CT-B showing three monoclonal antibody reaction patterns (epitypes), which cor...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-071
更新日期：1990-06-01 00:00:00
abstract：:Seven tk- mutants of herpes simplex virus, type 2 (HSV-2), and three tk- mutants of herpes simplex virus, type 1 (HSV-1), were isolated which did not produce the thymidine kinase (TK) polypeptides but formed smaller polypeptides not seen in wild-type infected cells. Positive TK mRNA selection by hybridization to the c...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-063
更新日期：1983-04-01 00:00:00
abstract：:Nitrogen-fixing, field-obtained root nodules of the silky green alder were studied by transmission electron microscopy. The nodule endophyte exhibited a prokaryotic cytology and was present in two forms: the hypha(0.3-1.0mum), which was branched and septate, and the vesicle (3-5mum), which was also septate and develo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-157
更新日期：1975-07-01 00:00:00
abstract：:Characterization and enumeration of the adherent epimural community of the rumen wall of young, conventionally reared lambs were carried out from 2 to 21 days after birth. Three hundred strains were isolated by anaerobic procedures from three sites: dorsal, ventral, and caudal sacs, and from the sloughed epithelial ce...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m89-114
更新日期：1989-07-01 00:00:00
abstract：:Proteins were solubilized from cell wall fractions of Candida albicans and separated by polyacrylamide gel electrophoresis. Cell walls were isolated from 25 and 37 degrees C growing and stationary phase yeast cultures and from germ tubes. The 42 protein bands detected by dye binding were observed in all wall extracts,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-220
更新日期：1983-10-01 00:00:00
abstract：:Plant secondary metabolites, such as saponins, have a considerable impact in agriculture because of their allelopathic effects. They also affect the growth of soil microorganisms, especially fungi. We investigated the influence of saponins on rhizosphere bacteria in vitro and in soil conditions. The effects of gypsoph...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-052
更新日期：2003-06-01 00:00:00
abstract：:We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in a dctA gene. Levels of dctA gene expression ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-008
更新日期：1992-01-01 00:00:00
abstract：:A 3.1-kb region of the bacteriophage D3 genome which contains the immunity functions has recently been sequenced (GenBank accession No. L22692). Sequence analysis indicated the presence of a putative repressor gene (c1) whose protein product functions to maintain the bacteriophage genome as a stably integrated prophag...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-030
更新日期：1997-03-01 00:00:00
abstract：:We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-102
更新日期：2007-01-01 00:00:00
abstract：:Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-070
更新日期：1996-05-01 00:00:00
abstract：:The presence of other soil microorganisms might influence the ability of rhizobacterial inoculants to promote plant growth either by reducing contact between the inoculant and the plant root or by interfering with the mechanism(s) involved in rhizobacterially mediated growth promotion. We conducted the following exper...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-080
更新日期：2001-09-01 00:00:00
abstract：:Increasing the use of nitrogen fertilizers in tea orchards has led to intense nitrous oxide (N2O) emissions. Foliar application of Paenibacillus polymyxa biofertilizer has been proven to be beneficial for organic tea production. In this study, tea yield and quality were significantly improved after application of P. p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2019-0511
更新日期：2020-03-01 00:00:00
abstract：:Numbers of viable bacteria as indicated by colony-forming units (CFU), microbial adenosine triphosphate levels, dissolved organic carbon (DOC), particulate organic carbon and nitrogen (POC and PON), glucose concentration, heterotrophic potential (Vmax), and in situ heterotrophic activity (Vn) of near-surface microlaye...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-251
更新日期：1976-12-01 00:00:00
abstract：:Attempts at improving chemical fixation for electron-microscopic observation of the capsule of Klebsiella pneumoniae were made. The capsule was preserved by using alcian blue - lanthanum and tris-(1-aziridinyl) phosphine oxide (TAPO) - aldehyde - osmium procedures. Despite the different retention of the overall capsul...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-102
更新日期：1977-06-01 00:00:00
abstract：:Studies of the molecular biology of human papillomavirus type 16 have been limited by the lack of a tissue culture system that is fully permissive for virus replication; as a result, high-titre stocks of infectious virus are not readily available. Therefore, studies of viral gene expression have relied on analysis of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-016
更新日期：1993-01-01 00:00:00
abstract：:The oligosaccharides of human lactoferrin were enzymatically removed with glycopeptidase F, resulting in a preparation containing partial and fully deglycosylated human lactoferrin. The derivatives were separated by Concanavalin A affinity chromatography and compared with native human lactoferrin with respect to their...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-198
更新日期：1992-11-01 00:00:00
abstract：:Nonlinear survivor curves were obtained when spores of Bacillus cereus were heated in physiological saline solution. Curvilinear survivor curves did not appear to be caused by experimental artifacts but by the heterogeneity of spore population with regard to heat resistance. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-217
更新日期：1975-10-01 00:00:00
abstract：:A strain of Pseudomonas putida grew rapidly on cyclohexanecarboxylic acid as a sole source of carbon. A CoA-mediated beta-oxidation pathway was induced for the metabolism of the compound. The organism could not utilize 3-cyclohexenecarboxylic acid as a sole source of carbon for growth, but cells grown on gluconate in ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-198
更新日期：1982-12-01 00:00:00
