The N-linked oligosaccharides of human lactoferrin are not required for binding to bacterial lactoferrin receptors.

Abstract:

:The oligosaccharides of human lactoferrin were enzymatically removed with glycopeptidase F, resulting in a preparation containing partial and fully deglycosylated human lactoferrin. The derivatives were separated by Concanavalin A affinity chromatography and compared with native human lactoferrin with respect to their ability to bind to bacterial receptors. Competitive binding experiments demonstrated that the lactoferrin derivatives were equally capable as native lactoferrin in binding to receptors of Neisseria meningitidis, Neisseria gonorrhoeae, and Moraxella catarrhalis. This result indicates that the oligosaccharides on human lactoferrin are not essential for binding to the bacterial receptors.

journal_name

Can J Microbiol

authors

Alcantara J,Padda JS,Schryvers AB

doi

10.1139/m92-198

subject

Has Abstract

pub_date

1992-11-01 00:00:00

pages

1202-5

issue

11

eissn

0008-4166

issn

1480-3275

journal_volume

38

pub_type

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