Abstract：
:Clinical isolates of Staphylococcus aureus were found to exhibit strain-specific heterogeneity to the growth-enhancing effects of human urokinase (UK), a proteinase with plasminogen activator activity. Nine out of fourteen (64%) methicillin-sensitive strains of S. aureus were responsive to UK in "in vitro" cultures. In contrast, 3/29 (10%) methicillin-resistant strains were responsive to the proteinase. When only strains isolated from western Canada were considered, 6/11 methicillin-sensitive strains and 1/26 methicillin-resistant strains were responsive to UK. The single western Canadian methicillin-resistant strain (strain 456) responsive to UK was one of two isolated from the same patient, indicating that the two strains were phenotypically different. Strain 456, resistant to 32 micrograms methicillin/mL, was responsive to as little as 50 U UK/mL and enhancement of growth was evident by 9 h of incubation at 37 degrees C. This growth enhancement was specific to UK and not duplicated by equivalent concentrations of other proteins (bovine serum albumin, trypsin, plasminogen). The results presented indicate differences in the frequency of the UK-responsive phenotype between methicillin-sensitive and -resistant S. aureus. These findings indicate that the UK phenotype of S. aureus may have utility in both phenotyping clinical isolates, as well as providing insights into the regulation of growth in this clinically important organism.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Hart DA,Louie T,Krulicki W,Reno Cdoi
10.1139/m96-131subject
Has Abstractpub_date
1996-10-01 00:00:00pages
1024-31issue
10eissn
0008-4166issn
1480-3275journal_volume
42pub_type
杂志文章abstract：:Brachiaria, predominantly an African genus, contains species, such as B. brizantha, an apomictic C4 grass, that are commercially important forage grasses in tropical America, where they now cover about 55 million hectares. From B. brizantha accession CIAT 6780, we isolated an endophytic fungus that may be economically...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-127
更新日期：2001-01-01 00:00:00
abstract：:Experiments were conducted to examine the dynamics of Cache Valley virus in Culiseta inornata, the probable chief vector of the virus. Of about 1500 laboratory reared C. inornata exposed to viraemic suckling mice, 72 took a blood meal. A relatively high precentage (93%) of the latter mosquitoes became infected. The vi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:Cultures of Sclerotium rolfsii and Trichoderma viride together in autoclaved soil were assayed at intervals during 8 days of incubation for proteolytic activity (PA) of T. viride. Significant proteolytic activity was detected only in soil containing T. viride (i.e., T. viride alone or S. rolfsii + T. viride); greatest...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-079
更新日期：1978-04-01 00:00:00
abstract：:Seven streptomycete strains were tested for biotransformation of salicylate. The products were identified by nuclear magnetic resonance spectroscopy and three types of conversion were found. Streptomyces cinnamonensis and Streptomyces spectabilis formed gentisate and salicylamide concurrently. Streptomyces rimosus tra...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-111
更新日期：1996-08-01 00:00:00
abstract：:Methanol extracts were obtained from L. interrogans serovars icterohaemorrhagiae and canicola and L. biflexa serovar patoc. Human sera from 167 normal individuals and 40 patients with different infectious diseases tested by complement fixation tests showed negative reactions. Sera from 100 patients with a suspicion of...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-075
更新日期：1991-06-01 00:00:00
abstract：:Nodulation of Pisum sativum cv. Afghanistan by Rhizobium leguminosarum strain Tom can be blocked by the nonnodulating R. leguminosarum strain PF2. This system was used to study the dynamics of rhizobial binding to seedling root surfaces. Appreciable numbers of 35S-labeled rhizobia bound to the root surface within 2h a...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-099
更新日期：1980-04-01 00:00:00
abstract：:Most prokaryotic chromosomes contain a number of toxin-antitoxin (TA) modules consisting of a pair of genes that encode 2 components, a stable toxin and its cognate labile antitoxin. TA systems are also known as addiction modules, since the cells become "addicted" to the short-lived antitoxin product (the unstable ant...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章,评审
doi：10.1139/w2012-025
更新日期：2012-05-01 00:00:00
abstract：:Immunovirological studies suggest that human respiratory syncytial virus may well be composed of five structural proteins as are other members of the Paramyxoviridae family: the two external membrane glycoproteins H (90 000) and Fo (F1, 49 000; F2, 20 000; disulfide linked), the internal membrane protein M (34 000), t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-004
更新日期：1986-01-01 00:00:00
abstract：:A species of Arthrobacter was recovered during culture of the causal organism of pitch canker of southern pines. Fusarium moniliforme var. subglutinans (FMS). Arthrobacter is a relatively common soil bacterium and is lytic to several fungal pathogens in the soil. Soil samples from two seed orchards with pitch canker a...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-004
更新日期：1981-01-01 00:00:00
abstract：:No clear data are available on how flavonoids from different chemical groups affect root colonization by arbuscular mycorrhizal fungi (AMF) and whether flavonoids affecting the presymbiotic growth of AMF also affect root colonization by AMF. In the present work, we compared the effect of flavones (chrysin and luteolin...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-036
更新日期：2007-06-01 00:00:00
abstract：:Guanidine-resistant (gr) mutants of poliovirus were previously categorized into four groups by electrophoretic properties and peptide maps of nonstructural virus protein 2C. Growth of mutants in the presence of guanidine depends upon temperature of incubation. The four groups of gr variants respond differently to temp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-220
更新日期：1985-12-01 00:00:00
abstract：:Separation of Vero cell membrane in a discontinuous sucrose gradient reveals five fractions. After infection a sixth fraction appears. It contains virioins but mostly modified membranes with subunits 5-6 nm in diameter, probably the hemagglutinin. None of the enzymes used was associated with this fraction. No modifica...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-103
更新日期：1975-05-01 00:00:00
abstract：:For the first time, an Escherichia coli strain producing four microcins (Mcc), B17, D93, J25, and L, and showing immunity to Mcc V was isolated and characterized. Each of the gene clusters encoding the production of Mcc B17, D93, and L was cloned separately. The gene cluster for Mcc L was cloned within a 13.5-kb HindI...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-047
更新日期：2003-05-01 00:00:00
abstract：:The monitoring of antimicrobial susceptibilities in Neisseria gonorrhoeae isolates and characterization of N. gonorrhoeae multiantigen sequence types (NG-MAST, ST) provide important surveillance data as resistance rates continue to rise. A total of 2970 N. gonorrhoeae isolates were collected by Canadian provincial pub...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0357
更新日期：2013-10-01 00:00:00
abstract：:Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-070
更新日期：1996-05-01 00:00:00
abstract：:Group B Neisseria meningitidis (SD1C) was grown on defined medium supplemented with each of a variety of sulphur compounds as the sole source of sulphur. The organism grew on sulphate, sulphite, bisulphite, thiosulphate, dithionite, hydrosulphide, thiocyanate, L-cysteine, L-cystine, reduced glutathione, methionine, me...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-232
更新日期：1984-12-01 00:00:00
abstract：:Probiotics are usually isolated from the gastrointestinal tract of humans and animals. The search of probiotics in human milk is a recent field of research, as the existence of the human milk microbiome was discovered only about a decade ago. To our knowledge, no reports regarding the potential probiotic effect of bac...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0811
更新日期：2016-06-01 00:00:00
abstract：:Of 24 bacterial species examined for lisinopril refractive peptidyl dipeptidase activity, only 8 contained activity. Activity in Pseudomonas maltophilia was more than fourfold higher than that of any other species. Pseudomonas maltophilia may be unique among bacteria in possessing high peptidyl dipeptidase activity th...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-010
更新日期：1990-01-01 00:00:00
abstract：:Species of Enterobacter and Serratia were examined for deoxyribonucleic acid relatedness to Klebsielleae, to atypical erwiniae, and to other members of Enterobacteriaceae. Deoxyribonucleic acid hybridization and then hydroxyapatite chromatography was the technique used to assess relatedness. Strains of Enterobacter cl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-018
更新日期：1976-02-01 00:00:00
abstract：:Subtyping Neisseria meningitidis by methods that rely on monoclonal antibody (mAb) reactivity results in an unusually high number of strains that are not subtypeable. To subtype 48 strains isolated (1993-1994) in the province of Quebec that were not subtypeable by mAb-based techniques, we used DNA sequencing of the va...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Candida albicans and Saccharomyces cerevisiae cells were examined by scanning scanning electron microscopy before and after extraction of the mannans of the cell wall. The surfaces of control cells were smooth; after mannan extraction they were rough and showed erosions which were particularly striking within the area...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-164
更新日期：1980-08-01 00:00:00
abstract：:The insect pathogenic fungus Aschersonia placenta is a highly effective pathogen of whiteflies and scale insects. However, few genetic tools are currently available for studying this organism. Here we report on the conditions for the production of transformable A. placenta protoplasts using an optimized protocol based...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0770
更新日期：2016-07-01 00:00:00
abstract：:Fifty-four strains of Neisseria gonorrhoeae were examined for their ability to be agglutinated by various lectins. Wheat germ agglutinin, ricin, soybean lectin, and peanut agglutinin agglutinated all strains tested. Dolichos biflorus, Sophora japonica, Maclura pomifera, Ulex Europaeus, Lens culinaris, Canavalia ensifo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-078
更新日期：1980-04-01 00:00:00
abstract：:Nitrogen-fixing, field-obtained root nodules of the silky green alder were studied by transmission electron microscopy. The nodule endophyte exhibited a prokaryotic cytology and was present in two forms: the hypha(0.3-1.0mum), which was branched and septate, and the vesicle (3-5mum), which was also septate and develo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-157
更新日期：1975-07-01 00:00:00
abstract：:The gene-encoding HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus JCM2501 was amplified and expressed in Escherichia coli and the enzymatic features were examined. The enzyme was detected mainly in the membrane fraction. It catalyzed the sulfide-dependent menaquinone (MK) reduction showing special enz...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-077
更新日期：2007-09-01 00:00:00
abstract：:Degradation of purified rubella virus by heat treatment (37, 45, or 56 degrees C) revealed the following structures. The viral envelope, a modified cellular membrane, bears spherical subunits, 5-6 nm in diameter, hexamers, or pentamers. Two glycoproteins, VP-2 (50 000 daltons) and VP-3 (63 000 daltons), are associated...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1975-05-01 00:00:00
abstract：:We report some characteristics of a ruminal bacterium (strain NPOH1) that metabolizes 3-nitropropanol, the toxic principle of various milk vetchs that are distributed worldwide. The gram-positive bacterium was nonmotile and did not produce spores. Growth of strain NPOH1 occurred under anaerobic conditions and was supp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-088
更新日期：1997-07-01 00:00:00
abstract：:In this work, we assayed the ability of newly prepared indolizine derivates (epimers) 6C and 6A to inhibit the growth of Mycobacterium smegmatis and used them for resistance induction. 6A inhibited the growth of M. smegmatis at a concentration of 100 μg/mL. No inhibitory effect was observed in the presence of 6C. By i...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0438
更新日期：2013-02-01 00:00:00
abstract：:The purpose of this study was to determine the prevalence of antibiotic-resistant bacteria and endotoxin in soil after land application of biosolids. Soil was collected over a 15 month period following land application of biosolids, and antibiotic resistance was ascertained using clinically relevant antibiotic concent...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-021
更新日期：2007-05-01 00:00:00
abstract：:The adherence of Streptococcus pneumoniae to epithelial (A549) lung cells was studied and the bacterial binding distribution was found to be nonrandom (non-Gaussian). Analysis of the dependency of bacterial binding on the cell cycle of A549 cells revealed that approximately 1.8 times more bacteria bind to G2 cells tha...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
