Abstract:
:The use of two pulsed lasers as excitation sources in the immunofluorescent technique FTA-ABS (fluorescent treponemal antibody absorption) shows that excitation of specimens by continuous or long-duration pulses of light (in the order of a millisecond) degrades the fluorescence in such a way that it is impossible to quantify exactly the fluorescent signal or repeat the analysis. Stability of fluorescence can be achieved by very short excitation pulses (in the order of a microsecond) and this fast fluorescent signal can be detected only by an electronic detection and recording system. With such a system, high precision is obtained by elimination of errors by the human eye.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Lambert NG,Ste-Marie ARsubject
Has Abstractpub_date
1975-08-01 00:00:00pages
1232-5issue
8eissn
0008-4166issn
1480-3275journal_volume
21pub_type
杂志文章abstract::When Caryophanon latum was exposed to egg white lysozyme in isotonic sucrose and observed by phase-contrast microscopy, protoplasts emerged along the length of the trichomes, apparently at sites corresponding to cross septa. Electron microscopy of sections revealed that this enzyme initially attacked the core of the s...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-024
更新日期:1975-02-01 00:00:00
abstract::Staphylococcus aureus isolated from the cervix of an infertile woman was found to cause complete immobilization of human spermatozoa in vitro. Only the cell culture and cell-free supernatant showed immobilization activity, indicating that the sperm immobilization factor might be released extracellularly by the organis...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-032
更新日期:2009-07-01 00:00:00
abstract::Three composts were tested for their ability to suppress root and stem rot caused by the soil borne fungal pathogen Fusarium oxysporum f. sp. radicis-cucumerinum (FORC) on cucumber. Two of the composts were prepared from separated dairy solids either by windrow (WDS) or vermicomposting (VMC) while the third, obtained ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-082
更新日期:2000-11-01 00:00:00
abstract::Polymyxin-resistant (PBLr) mutants of Agrobacterium tumefaciens A6, B6, and B6M were isolated from polymyxin-sensitive (PBLs) parent strains in a defined medium containing 600 microgram of polymyxin B sulfate per millilitre. The weight and number of tumors induced by PBLr mutants on a variety of host plants such as ca...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-029
更新日期:1979-02-01 00:00:00
abstract::We continued our study of high-molecular-mass proteases (HMMPs) using several strains of the genus Trichoderma, and other filamentous fungi (Botrytis cinerea, Aspergillus niger, Fusarium culmorum, and Penicillium purpurogenum). We found that five Trichoderma strains secreted HMMPs into the media after induction with b...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0670
更新日期:2019-09-01 00:00:00
abstract::Colletotrichum gloeosporioides is the causal agent of rubber tree anthracnose and leads to serious losses of natural rubber production. The pathogenesis of C. gloeosporioides on rubber tree remains unknown. Cerato-platanin proteins are small, secreted cysteine-rich proteins that contribute to virulence and function in...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0087
更新日期:2018-11-01 00:00:00
abstract::A visual micromethod for measuring antifungal effects on germination and growth is described. The antifungal agent griseofulvin and the fungus Trichophyton mentagrophytes were used as materials to compare the micromethod with a standard assay based on dry mycelial weight. The micromethod was more sensitive than the we...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-093
更新日期:1978-05-01 00:00:00
abstract::The physiochemical characteristics of the intracellular proteolytic enzymes of Oidiodendron kalari, a neuropathogenic fungus, were studied. The organism in the yeast phase was grown in a semisynthetic medium containing 1% tryptone, at 37 degrees C for 48 hr, on a gyrotory shaker. The crude extract was prepared by brea...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-204
更新日期:1975-09-01 00:00:00
abstract::Rhizobacteria promote and have beneficial effects on plant growth, making them useful to agriculture. Nevertheless, the rhizosphere of the chickpea plant has not been extensively examined. The aim of the present study was to select indole-3-acetic acid (IAA) producing rhizobacteria from the rhizosphere of chickpea pla...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0399
更新日期:2014-10-01 00:00:00
abstract::A tryptophan auxotroph of aerobically grown Rodospirillum rubrum was isolated after mutagenesis and replica plating. The mutant grows on tryptophan or indole, accumulates indole glycerol phosphate, lacks the capacity to convert indole glycerol phosphate to indole glycerol phosphate to indole, and finally is defective ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-055
更新日期:1978-03-01 00:00:00
abstract::Shiga-toxin-producing Escherichia coli (STEC) represent a major concern for waterborne disease outbreaks associated with consumption of contaminated groundwater. Over 4 million people rely on private groundwater systems as their primary drinking water source in Canada; many of these systems do not meet current standar...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/cjm-2019-0329
更新日期:2020-05-01 00:00:00
abstract::Experiments were carried out to elucidate linkage between methane consumption and mineralization of P from different phosphorous sources. The treatments were no CH4 no P amendment absolute control, with CH4 no P amendment control, with CH4 + inorganic P as Ca3(PO4)2 and with CH4 + organic P (sodium phytate). P sources...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2020-0254
更新日期:2020-12-10 00:00:00
abstract::Environmental factors influencing the occurrence and community structure of soil yeasts in forests are not well studied. There are few studies dedicated to Southern Hemisphere soil yeasts populations and even fewer focused on temperate forests influenced by volcanic activity. The present work aimed to study the ecolog...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0897
更新日期:2014-06-01 00:00:00
abstract::The diversity of 16 strains of chickpea-infective rhizobia from various geographical origins was analysed using genotypic and phenotypic approaches. Multilocus enzyme electrophoresis was performed, and restriction fragment length polymorphisms of the amplified 16S + IGS (intergenic spacer) rRNA gene, assimilation of 1...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m94-057
更新日期:1994-05-01 00:00:00
abstract::The effect of phenolic compounds, ethyl alcohol, and sodium metabisulphite on the lytic activity of virulent bacteriophage PL-1 on a Lactobacillus casei S strain isolated from a lactic acid beverage fermentation was investigated. Catechin, caffeic, and gallic acids, commercially produced red, white, and champagne tann...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-162
更新日期:1985-09-01 00:00:00
abstract::We studied the persistence of naturally acquired cell-mediated immunity to rubella during early pregnancy. We compared lymphocyte transformation responses to phytohemagglutinin and rubella virus in 35 naturally immune women aged 17-37 years, in the first trimester of pregnancy, with 49 naturally immune age-matched non...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-257
更新日期:1983-12-01 00:00:00
abstract::Ten aspergilli (five each from marine and terrestrial habitats) were screened for siderophore production. All test isolates produced siderophores as indicated by a positive reaction in the FeCl(3) test, chrome azurol sulphonate assay, and chrome azurol sulphonate agar plate test. Further, the test isolates were compar...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-012
更新日期:2006-06-01 00:00:00
abstract::Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-038
更新日期:2004-08-01 00:00:00
abstract::The natural reservoirs and biological characteristics of pathogenic populations of both subspecies of Photobacterium damselae in aquatic habitants remain unclear because of difficulties in obtaining pathogenic strains from the environment. In the present study, we assessed the occurrence of Photobacterium damselae sub...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w11-021
更新日期:2011-05-01 00:00:00
abstract::Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-070
更新日期:1996-05-01 00:00:00
abstract::Ten bacterial strains were isolated by enrichment culture, using as carbon sources either aliphatics or an aromatic-polar mixture. Oxygen uptake rate was used as a criterion to determine culture transfer timing at each enrichment stage. Biodegradation of aliphatics (10,000 mg L(-1)) and an aromatic-polar mixture (5000...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-098
更新日期:2003-12-01 00:00:00
abstract::A method for the production of conjugated linoleic acid (CLA) from linoleic acid (LA) using growing cultures of Propionibacterium freudenreichii ssp. shermanii JS was developed. The growth inhibitory effect of LA was eliminated by dispersing it in a sufficient concentration of polyoxyethylene sorbitan monooleate deter...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::Three composts (Ball, dairy, and greenhouse) were tested for the ability to suppress the development of Fusarium root and stem rot (caused by Fusarium oxysporum f. sp. radicis-cucumerinum) on greenhouse cucumber. Dairy and greenhouse composts significantly reduced disease severity (P = 0.05), while Ball compost had no...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-076
更新日期:2010-11-01 00:00:00
abstract::Melanin biosynthesis in Verticillium dahliae Kleb, was studied with mutants deficient for normal black melanin or for production of microsclerotia. Seven genetically different mutants had apparent blocks in melanin biosynthesis. Four mutants (brm-1 to -4) produced brown microsclerotia and extruded pigments into media;...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-115
更新日期:1976-06-01 00:00:00
abstract::Chitinases isolated from membrane and cytosolic fractions of two mucoraceous fungi, Choanephora cucurbitarum and Phascolomyces articulosus, were investigated. The membrane-bound chitinase was isolated by Bio-Gel P-100 and DEAE Bio-Gel A chromatographic techniques. On SDS-PAGE the chitinase from both fungi migrated as ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-056
更新日期:1992-04-01 00:00:00
abstract::Five strains of Haemophilus pleuropneumoniae, out of eight strains tested, produced extracellular haemolysin(s) when grown in liquid culture in the presence, but not in the absence, of RNA. The haemolysin produced by the neotype strain was unstable, heat labile, and sensitive to degradation by pronase, trypsin, and ch...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-085
更新日期:1985-05-01 00:00:00
abstract::Like recF and recQ mutants studied earlier, two other classes of Escherichia coli mutants defective in the RecF conjugal recombination pathway, recJ and recO, were found to be partially resistant to thymineless death. In contrast, a recN mutant, also belonging to the pathway, was indistinguishable from the wild type w...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-157
更新日期:1988-07-01 00:00:00
abstract::The effect of atmospheric composition and storage temperature on growth and survival of uninjured and sublethally heat-injured Escherichia coli O157:H7, inoculated onto brain heart infusion agar containing 0.3% beef extract (BEM), was determined. BEM plates were packaged in barrier bags in air, 100% CO2, 100% N2, 20% ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w98-224
更新日期:1999-03-01 00:00:00
abstract::Pseudomonas aeruginosa establishes life-long chronic infections in the cystic fibrosis (CF) lung by utilizing various adaptation strategies. Some of these strategies include altering metabolic pathways to utilize readily available nutrients present in the host environment. The airway sputum contains various host-deriv...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0485
更新日期:2014-12-01 00:00:00
abstract::Nonmotile and motility-altered mutants of Myxococcus xanthus have been obtained by the use of chemical mutagens, ultraviolet irradiation, and a procedure for selective spontaneous mutants. As judged by their behaviour on a variety of growth media, in both plate and slide culture, the mutants were divided into four gro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-190
更新日期:1976-09-01 00:00:00