[Study of the stability and the degradation of fluorescence in the immunofluorescence technic (FTA-ABS) using pulsed lasers].

Abstract:

:The use of two pulsed lasers as excitation sources in the immunofluorescent technique FTA-ABS (fluorescent treponemal antibody absorption) shows that excitation of specimens by continuous or long-duration pulses of light (in the order of a millisecond) degrades the fluorescence in such a way that it is impossible to quantify exactly the fluorescent signal or repeat the analysis. Stability of fluorescence can be achieved by very short excitation pulses (in the order of a microsecond) and this fast fluorescent signal can be detected only by an electronic detection and recording system. With such a system, high precision is obtained by elimination of errors by the human eye.

journal_name

Can J Microbiol

authors

Lambert NG,Ste-Marie AR

subject

Has Abstract

pub_date

1975-08-01 00:00:00

pages

1232-5

issue

8

eissn

0008-4166

issn

1480-3275

journal_volume

21

pub_type

杂志文章
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  • Isolation of a spermatozoal immobilization factor from Staphylococcus aureus filtrates.

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  • The study of intracellular and secreted high-molecular-mass protease(s) of Trichoderma spp., and their responses to conidiation stimuli.

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  • A Colletotrichum gloeosporioides cerato-platanin protein, CgCP1, contributes to conidiation and plays roles in the interaction with rubber tree.

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  • Visual micromethod for assay of fungal growth.

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    doi:10.1139/m78-093

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  • Proteolytic activity of Oidiodendron kalrai.

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  • IAA-producing rhizobacteria from chickpea (Cicer arietinum L.) induce changes in root architecture and increase root biomass.

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  • Evaluating the risks associated with Shiga-toxin-producing Escherichia coli (STEC) in private well waters in Canada.

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  • Ecology of cultivable yeasts in pristine forests in northern Patagonia (Argentina) influenced by different environmental factors.

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  • Genotypic and phenotypic diversity of Rhizobium isolated from chickpea (Cicer arietinum L.).

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    doi:10.1139/m94-057

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  • Effect of phenolic compounds, ethyl alcohol, and sodium metabisulphite on the lytic activity of phage PL-1 on a Lactobacillus casei S strain.

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  • Persistence of naturally acquired cell-mediated immunity to rubella virus during the first trimester of pregnancy.

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    doi:10.1139/m83-257

    authors: Rossier E,Phipps PH,Weber JM

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  • Chemical characterization and quantification of siderophores produced by marine and terrestrial aspergilli.

    abstract::Ten aspergilli (five each from marine and terrestrial habitats) were screened for siderophore production. All test isolates produced siderophores as indicated by a positive reaction in the FeCl(3) test, chrome azurol sulphonate assay, and chrome azurol sulphonate agar plate test. Further, the test isolates were compar...

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  • Isolation and transcriptional regulation of the Kluyveromyces lactis FBA1 (fructose-1,6-bisphosphate aldolase) gene.

    abstract::Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is ...

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  • Cytosolic and membrane-bound chitinases of two mucoraceous fungi: a comparative study.

    abstract::Chitinases isolated from membrane and cytosolic fractions of two mucoraceous fungi, Choanephora cucurbitarum and Phascolomyces articulosus, were investigated. The membrane-bound chitinase was isolated by Bio-Gel P-100 and DEAE Bio-Gel A chromatographic techniques. On SDS-PAGE the chitinase from both fungi migrated as ...

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  • Thymineless death in Escherichia coli mutants deficient in the RecF recombination pathway.

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