Abstract:
:Pseudomonas aeruginosa establishes life-long chronic infections in the cystic fibrosis (CF) lung by utilizing various adaptation strategies. Some of these strategies include altering metabolic pathways to utilize readily available nutrients present in the host environment. The airway sputum contains various host-derived nutrients that can be utilized by P. aeruginosa, including phosphatidylcholine, a major component of lung surfactant. Pseudomonas aeruginosa can degrade phosphatidylcholine to glycerol and fatty acids to increase the availability of usable carbon sources in the CF lung. In this study, we show that some CF-adapted P. aeruginosa isolates utilize glycerol more efficiently as a carbon source than nonadapted isolates. Furthermore, a mutation in a gene required for glycerol utilization impacts the production of several virulence factors in both acute and chronic isolates of P. aeruginosa. Taken together, the results suggest that interference with this metabolic pathway may have potential therapeutic benefits.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Daniels JB,Scoffield J,Woolnough JL,Silo-Suh Ldoi
10.1139/cjm-2014-0485subject
Has Abstractpub_date
2014-12-01 00:00:00pages
857-63issue
12eissn
0008-4166issn
1480-3275journal_volume
60pub_type
杂志文章abstract::A visual micromethod for measuring antifungal effects on germination and growth is described. The antifungal agent griseofulvin and the fungus Trichophyton mentagrophytes were used as materials to compare the micromethod with a standard assay based on dry mycelial weight. The micromethod was more sensitive than the we...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-093
更新日期:1978-05-01 00:00:00
abstract::Scanning and transmission electron microscopy was applied for a morphological study of three strains of Bifidobacterium grown on solid or liquid media. The pronounced pleomorphism of the cultures previously observed by light microscopy was confirmed. A possible sequence of the morphological events during transformatio...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-197
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abstract::To determine the seroprevalence of Norwalk virus and whether Norwalk virus contributed to an observed increase in illness in tap water drinkers participating in a prospective epidemiological study, sera collected during the study were examined for changes in Norwalk virus antibody titer, using a specific enzyme immuno...
journal_title:Canadian journal of microbiology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1994-10-01 00:00:00
abstract::Seven lambs, separated from their dam 24 h after birth, were kept in a conventional environment until transferred to sterile isolators between 1 and 9 days of age: two on day 1 (IA and IB), two on day 4 (IVA and IVB), one on day 8 (VIIIA), and two on day 9 (IXA and IXB). The lambs were reared in these isolators until ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-064
更新日期:1989-03-01 00:00:00
abstract::The effects of pressurized CO2 on the survival of Escherichia coli and the mechanism of cell inactivation were studied. Bacterial cultures were inoculated in nutrient broth and incubated at 30 degrees C for 18 h. Exposure of the cells to CO2 under pressures ranging from 2.5 to 25 MPa and at temperatures between 8 and ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-078
更新日期:2006-12-01 00:00:00
abstract::Acid (AcP) and alkaline phosphatase (AlP) were localized by physicobiochemical techniques. Greater than 53% of the phosphatases were detected, following sonication, in a low speed centrifugation pellet while osmotically shocked and spheroplasted Capnocytophaga species released only 9-28% and 11-43% of the cellular pho...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-212
更新日期:1983-10-01 00:00:00
abstract::The synthesis of the nucleotide precursors for peptidoglycan is regulated by the relA gene in Escherichia coli. Thus, nucleotide precursors labeled with [3H]diaminopimelic acid accumulated in a relA strain but not in an isogenic relA+ strain during amino acid deprivation. Furthermore, nucleotide precursor synthesis wa...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-098
更新日期:1982-06-01 00:00:00
abstract::The influence of plant growth promoting (PGP) activity of bacterial communities recovered from each of six weed species (barnyard grass (Echinochloa crusfalli (L.) Beauv.), corn spurrey (Spergula arvensis L.), goldenrod (Sonchus sp.), Italian ryegrass (Lolium multiflorum L.), lamb's-quarters (Chenopodium album L.), an...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-110
更新日期:2001-11-01 00:00:00
abstract::Southern bean mosaic virus (SBMV) protein was cleaved with cyanogen bromide and a highly basic peptide, CB-1, was isolated by ion exclusion and ion-exchange chromatography. Twelve peptides were separated from a tryptic digest of CB-1 by ion-exchange chromatography and the composition of these peptides was similar to t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-241
更新日期:1980-12-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-062
更新日期:2002-08-01 00:00:00
abstract::The aim of this study was to assess the influence of sodium content on the microbiota on the surface of ready-to-eat (RTE) meat products purchased from the retail market in Canada. Products, including sliced and sausage-type deli meats, were analysed with culture-dependent and culture-independent methods. Bacteria wer...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0630
更新日期:2015-02-01 00:00:00
abstract::Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w98-225
更新日期:1999-02-01 00:00:00
abstract::Marine shrimp of the species Penaeus monodon were collected from the coastal region (Haroa) of the deltaic Sundarbans of West Bengal, India during the premonsoon period in 1996. Klebsiella pneumoniae was isolated from the alimentary canal and gills of the shrimp as the sole isolate. All 10 isolated strains were resist...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
abstract::Of 33 streptococcal cultures belonging to serological group L, all bound human immunoglobulin (Ig) G, fibrinogen, and fibronectin; 32 bound bovine IgG; 31 bound alpha 2-macroglobulin; 5 bound albumin; and none bound either haptoglobin or IgA. The binding sites for IgG could be isolated from the L streptococci by tryps...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-001
更新日期:1988-01-01 00:00:00
abstract::In this study, 16S rRNA gene primers were designed to complement the suite of already available PCR primers for the detection of different methanogens involved in biogas production through anaerobic digestion by SYBR Green real-time PCR. Primers designed for use in TaqMan real-time PCR for the organisms Methanosaeta, ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-157
更新日期:2009-05-01 00:00:00
abstract::Long-term survival, dispersal, and germination of Bacillus thuringiensis var. kurstaki DMU67R has been investigated in a field trial. An experimental cabbage plot was sprayed with DMU67R in 1993 and allowed to lie fallow since. The investigations reported here were carried out from 1997 to 2000 in this plot. High pers...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-009
更新日期:2002-03-01 00:00:00
abstract::The cellular functions are strongly influenced by the composition of the environment. In particular, phenotypes of microbial strains are modulated by concentrations of ions in the culture medium, and differences in element levels may be responsible for a phenotypic variability observed when microbial strains are grown...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-012
更新日期:2007-04-01 00:00:00
abstract::Actinomycetes DNAs were digested with restriction enzymes to study the presence of methylated bases. Analysis showed that the enterobacterial Dam and Dcm systems are absent. Methylation at the internal cytosine in CCGG sequences, typical of eukaryotes, was also absent. We also tested 18 restriction endonucleases recog...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-031
更新日期:1996-02-01 00:00:00
abstract::Most prokaryotic chromosomes contain a number of toxin-antitoxin (TA) modules consisting of a pair of genes that encode 2 components, a stable toxin and its cognate labile antitoxin. TA systems are also known as addiction modules, since the cells become "addicted" to the short-lived antitoxin product (the unstable ant...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章,评审
doi:10.1139/w2012-025
更新日期:2012-05-01 00:00:00
abstract::A disseminated and fatal infection was established in C57BL mice, injected intraperitoneally with either Neisseria meningitidis B,2b or Haemophilus influenzae type b bacteria plus enhancement factors. The effects of mucin, hemoglobin, and iron dextran as enhancement of bacterial infectivity in mice were evaluated indi...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-007
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-148
更新日期:1976-07-01 00:00:00
abstract::Deoxyribonuclease (DNase) activity was found in the culture fluids of numerous marine bacteria isolated from seawater. Among these ogranisms, marine bacterium, Vibrio sp., strain No. 2, showed the highest deoxyribonucleic acid-hydrolyzing activity. This organism requires salts of seawater for both growth and extracell...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-213
更新日期:1976-10-01 00:00:00
abstract::In mycetomes of leafhoppers, Helochara communis, ultrastructural and histochemical studies revealed that spheroplast symbiotes (t-symbiotes) were converted to 'a-symbiotes' (so-called), with apparent loss of DNA--a phenomenon reminiscent of rhizobium-bacteroid conversion in certain legume nodules. Additional t-symbiot...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-028
更新日期:1975-02-01 00:00:00
abstract::The sensitivity of delta red-gam delta ren mutants of bacteriophage lambda to Rex exclusion by lambda rexA+ rexB+ lysogens is modulated by the prophage cI repressor allele. We show the following: (i) lambda spi156 delta nin5 forms plaques on a cI+-rexA+-rexB+ lysogen with 10(5)-fold higher efficiency than on cI[Ts]-re...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w03-021
更新日期:2003-03-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-106
更新日期:1983-06-01 00:00:00
abstract::Large numbers of minute, flat, long, straight, but often broken structures were observed in aqueous extracts of soil. We have applied the purely descriptive term 'tubules' to these structure, because they apparently became flattened during preparation for electron-microscopic viewing. The tubules appeared to be compos...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
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更新日期:2010-02-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w10-054
更新日期:2010-08-01 00:00:00
abstract::Immunovirological studies suggest that human respiratory syncytial virus may well be composed of five structural proteins as are other members of the Paramyxoviridae family: the two external membrane glycoproteins H (90 000) and Fo (F1, 49 000; F2, 20 000; disulfide linked), the internal membrane protein M (34 000), t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-004
更新日期:1986-01-01 00:00:00