Abstract：
:Characterization and enumeration of the adherent epimural community of the rumen wall of young, conventionally reared lambs were carried out from 2 to 21 days after birth. Three hundred strains were isolated by anaerobic procedures from three sites: dorsal, ventral, and caudal sacs, and from the sloughed epithelial cells. The population of epimural bacteria was very dense from the first days of the lamb's life. This population increased slightly with age. During the first week the counts were similar in the dorsal and ventral sacs, but they were 10 to 100 times lower in the caudal sac. Total counts for anaerobic bacteria were higher than the counts for aerobic bacteria. The isolated strains were distributed into 19 groups: 11 groups included aerotolerant strains, and 8 others, strictly anaerobic strains. During the first week the facultative microflora was mainly composed of Escherichia coli and Streptococci. Later, the epimural community was more complex and included Staphyloccus, Micrococcus, and Gaffkya. The strictly anaerobic microflora was mainly composed of Clostridium, Peptostreptococcus, Veillonella, Propionibacterium, and Acidaminococcus. Some of these strains appeared to be similar to those previously isolated from the rumen fluid of young lambs; however, the genera Micrococcus, Veillonella, Gaffkya, and Acidaminococcus, and E. coli seemed to be specific of the rumen wall tissues.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Rieu F,Fonty G,Gouet Pdoi
10.1139/m89-114subject
Has Abstractpub_date
1989-07-01 00:00:00pages
698-705issue
7eissn
0008-4166issn
1480-3275journal_volume
35pub_type
杂志文章abstract：:A 3.1-kb region of the bacteriophage D3 genome which contains the immunity functions has recently been sequenced (GenBank accession No. L22692). Sequence analysis indicated the presence of a putative repressor gene (c1) whose protein product functions to maintain the bacteriophage genome as a stably integrated prophag...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-030
更新日期：1997-03-01 00:00:00
abstract：:Subtyping Neisseria meningitidis by methods that rely on monoclonal antibody (mAb) reactivity results in an unusually high number of strains that are not subtypeable. To subtype 48 strains isolated (1993-1994) in the province of Quebec that were not subtypeable by mAb-based techniques, we used DNA sequencing of the va...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Several restriction systems were detected in different strains of Streptomyces antibioticus by using actinophages as biological indicators. Adsorption of phages to the bacteria, together with the study of the efficiency of plating gave an initial indication of restriction in three strains. The alternation of efficienc...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-177
更新日期：1985-10-01 00:00:00
abstract：:Five strains of Haemophilus pleuropneumoniae, out of eight strains tested, produced extracellular haemolysin(s) when grown in liquid culture in the presence, but not in the absence, of RNA. The haemolysin produced by the neotype strain was unstable, heat labile, and sensitive to degradation by pronase, trypsin, and ch...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-085
更新日期：1985-05-01 00:00:00
abstract：:To isolate thiamine excretors and (or) overproducers, 188 cultures belonging to nine yeast and three fungal genera were screened. Nine excreted thiamine as determined by both the presence of cross-feeding zones on a thiamine-free agar medium seeded with a thiamine-requiring yeast strain and by the direct detection of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-189
更新日期：1992-11-01 00:00:00
abstract：:Several physiological groups of bacteria were examined for the presence of a cytochrome b - like pigment which is demonstrable in dithionite-reduced minus substrate-reduced difference spectra. This pigment is characterized by an unusually high alpha band at 567 nm, a low concentration relative to conventional cytochro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-177
更新日期：1983-09-01 00:00:00
abstract：:Rabbit antisera prepared to meningococcal serogroups Y and W135 strains were compared with horse antisera using the antiserum agar method (ASA) for the serogroup identification of Neisseria meningitidis. Thirty-seven group Y strains formed immunoprecipitates with the Y rabbit serum only, whereas the same Y strains for...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-109
更新日期：1980-05-01 00:00:00
abstract：:A test is described for selective inhibition of Mycobacterium tuberculosis and M. bovis isolates in fluid medium. The method employs rho-nitro-alpha-acetyl-amino-beta-hydroxy-propiophenone (NAP) as an inhibitory agent for differentiation of mammalian tuberculosis strains from other Mycobacteria. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-124
更新日期：1978-06-01 00:00:00
abstract：:Trace gas uptake by microorganisms controls the oxidative capacity of the troposphere, but little is known about how this important function is affected by changes in soil microbial diversity. This article bridges that knowledge gap by examining the response of the microbial community-level physiological profiles (CLP...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2019-0412
更新日期：2020-04-01 00:00:00
abstract：:A technique is described that employs vapor fixation and a simple vapor diffusion dehydration apparatus to minimize the disturbance of delicate fungal structures during preparation for scanning electron microscopy. The technique is applicable to fungi grown on agar media or on natural substrates. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-106
更新日期：1983-06-01 00:00:00
abstract：:To screen, isolate, and characterize bacterial populations producing microcin J25, we report here two rapid, reliable, and sensitive methods, using polymerase chain reaction and colony blot hybridization with a digoxigenin-labelled probe. A sample of 26 Escherichia coli strains isolated from poultry intestinal content...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:The objective of the present work was to evaluate the relevance of the 2-methylcitric acid cycle (2MCC) to the catabolism of propionate in Burkholderia sacchari. Two B. sacchari mutants unable to grow on propionate were obtained: one disrupted in acnM, and the other in acnM and prpC deleted. An operative 2MCC signific...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-018
更新日期：2009-06-01 00:00:00
abstract：:A morphological and physiological description of an anamorphic basidiomycetous yeast species, named Trichosporon guehoae (CBS 8521T), is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w99-055
更新日期：1999-08-01 00:00:00
abstract：:The use of hsp60 gene sequences for phylogenetic study and identification of pathogenic marine vibrios was investigated. A 600-bp partial hsp60 gene was amplified by PCR and sequenced from 29 strains representing 15 Vibrio species within the family Vibrionaceae. Sequence comparison of the amplified partial hsp60 gene ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w02-089
更新日期：2002-10-01 00:00:00
abstract：:The study of the relationship between MVL 51 virus, of the Gourlay group L 1, and the Mycoplasma cells of A. laidlawii during the first 35 min of infection leads to the conclusion that a "carrier state" has been established, free virus as well as infected and noninfected cells being present at the same time in the cul...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:Intestinal infections are a major cause of mortality in domestic rabbits. Although a few pathogenic organisms have been associated with this type of infection, it is often impossible to demonstrate the presence of any etiologic agents. Recently viral particles were observed in the intestine of diseased rabbits submitt...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-201
更新日期：1980-10-01 00:00:00
abstract：:Agricultural practices such as manure applications could contribute to the spread of antibiotic resistance genes (ARGs) within the environment. Our objective was to assess the impact of certain fertilization methods (mineral or manure) and tillage practices (reduced or conventional) on the presence of ARGs and bacteri...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2019-0343
更新日期：2020-10-01 00:00:00
abstract：:Microsclerotia of three melanin-deficient mutants of Verticillium dahliae formed malanin from (+)-scytalone, 1,8-dihydroxynaphthalene, catechol, and L-3,4-dihydroxyphenylalanine. The melanins formed from (+)-scytalone or 1,8-dihydroxynaphthalene resembled wild-type melanin chemically and ultrastructurally, whereas the...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-049
更新日期：1978-03-01 00:00:00
abstract：:The biochemical reactions, carbohydrate content, and 16S-rRNA sequences of Tatlockia (Legionella) maceachernii and Tatlockia micdadei strains were studied. Except for catalase activity, Tatlockia strains were relatively inert in the biochemical tests commonly used in clinical laboratories. Phenotypically, the two Tatl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-069
更新日期：1993-05-01 00:00:00
abstract：:Recent food-borne outbreaks of human listeriosis as well as numerous sporadic cases have been mainly caused by Listeria monocytogenes serovar 4b strains. Thus, it was of interest to find out whether a certain clone or a certain few clones were responsible for these cases and especially for outbreaks. We used pulsed-fi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-058
更新日期：1993-04-01 00:00:00
abstract：:Absorption of 14C-labelled poliovirus-2 to sedimentable solids of primary sludge samples collected from a secondary treatment facility during a 6-month period averaged 94%; for anaerobically digested sludge, 99%. The extent of virus adsorption was influenced by the amount of solids. Maximal adsorption occurred at or a...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-254
更新日期：1983-12-01 00:00:00
abstract：:Cultures of Bacillus subtilis lysogenic for the temperature bacteriophage SP beta release "betacin", a bacteriocinlike substance that inhibits B. subtilis strains which do not carry this phage. Production of betacin is blocked by mutations in the bet gene on the prophage and a second phage gene, tol, is apparently inv...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-220
更新日期：1980-11-01 00:00:00
abstract：:Early investigation into the mechanism of mitochondriogenesis in the facultative anaerobe Saccharomyces cerevisiae used anaerobic-aerobic transitions as model systems to study the in vivo assembly of these oxidative organelles. This methodology has become entrenched in the early literature and as a result a definitive...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-126
更新日期：1975-06-01 00:00:00
abstract：:Actinomycetes DNAs were digested with restriction enzymes to study the presence of methylated bases. Analysis showed that the enterobacterial Dam and Dcm systems are absent. Methylation at the internal cytosine in CCGG sequences, typical of eukaryotes, was also absent. We also tested 18 restriction endonucleases recog...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-031
更新日期：1996-02-01 00:00:00
abstract：:Schizophyllum commune Fr. was shown, by light, scanning, and transmission electron microscopy, to be a destructive mycoparasite on several phytopathogenic and nematode-trapping fungi. The hyphae of S. commune coiled around host hyphae and fruiting structures and penetrated them by means of either unspecialized hyphae ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-131
更新日期：1978-07-01 00:00:00
abstract：:The repair of DNA damage caused by ultraviolet radiation (UVR) is well understood in both lower and higher organisms. Genetic studies carried out at optimum temperature for growth, 37 °C in Escherichia coli, have revealed the major pathways of DNA repair. We show that E. coli cells grown at 20 °C are more sensitive to...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0874
更新日期：2014-05-01 00:00:00
abstract：:The acetylene-reduction assay was used for in situ and laboratory assessment of biological nitrogen fixation in the acidic, waterlogged, muskeg ecosystem of the southern James Bay area, in the region of Moosonee, Ontario. In situ assays and subsequent laboratory experimentation revealed that nitrogenase activity was p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-130
更新日期：1976-07-01 00:00:00
abstract：:The diversity and variation of total and active ammonia-oxidizing bacteria in a full-scale aerated submerged biofilm reactor for drinking water pretreatment were characterized by clone libraries and denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA and its gene during a whole year. Sequences obtained...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-011
更新日期：2008-05-01 00:00:00
abstract：:The aim of the present study was to explore a new approach based on the hemagglutination (HA) assay to understand the colonization ability of Shigella spp. To study colonization ability, an animal model of 4-day-old suckling mouse, was exploited. We characterized the HA activity of 48 Shigella strains, with erythroc...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-095
更新日期：2012-10-01 00:00:00
abstract：:Humicola lutea 103 is a copper-tolerant fungal strain able to grow in the presence of 300 μg·mL(-1) Cu(2+) under submerged cultivation. To prevent the consequences of copper overload, microorganisms have evolved molecular mechanisms that regulate its uptake, intracellular traffic, storage, and efflux. In spite of this...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-112
更新日期：2012-12-01 00:00:00
