Abstract:
:A virulent strain of pigeon pea wilt pathogen was isolated from wilted pigeon pea plants and was identified as Fusarium oxysporum f. sp. udum. Many bacterial cultures showing antagonism to the pathogen were isolated from various ecological niches. When tested under pot and field conditions, development of fusarial wilt symptoms was prevented in pigeon pea seeds treated with one such antagonist, Bacillus brevis. A formulation of B. brevis with vermiculite as a carrier had a shelf life of at least 6 months. Bacillus brevis produced an extracellular antagonistic substance which induced swelling of the pathogen's hyphal tips, and cells were bulbous and swollen with shrunken and granulated cytoplasm. The antagonistic substance also inhibited germination of conidia, and was fungicidal to the vegetative mycelia of the pathogen. Comparison of the properties of our antagonistic substance with that of known antibiotics produced by B. brevis suggests that our antagonistic substance is a novel compound. The observations reported here indicate that this strain of B. brevis may have potential as a biocontrol agent against fusarial wilt in pigeon pea.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Bapat S,Shah AKdoi
10.1139/w99-109subject
Has Abstractpub_date
2000-02-01 00:00:00pages
125-32issue
2eissn
0008-4166issn
1480-3275journal_volume
46pub_type
杂志文章abstract::Trichloroethylene (TCE) is a prevalent contaminant of groundwater that can be cometabolically degraded by indigenous microbes. Groundwater contaminated with TCE from a US Department of Energy site in Ohio was used to characterize the site-specific impact of phenol on the indigenous bacterial community for use as a pos...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-025
更新日期:2005-06-01 00:00:00
abstract::Dithiothreitol reacted, at pH 9.0, with the isolated cell walls of Methanospirillum hungatii, to release about 23% of the cell wall dry weight as a high molecular weight fraction (> 0.5 million daltons). Untreated walls consisted of 70% amino acids, 11% lipid, and 6.6% carbohydrate. Sugars were identified as rhamnose,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-017
更新日期:1980-02-01 00:00:00
abstract::The application of crossed immunoelectrophoresis to the analysis of a reference cytoplasm and cell envelope preparation from Pasteurella multocida serotype 1 revealed antigenic complexity not previously found. At least 55 cytoplasmic and 19 cell envelope antigens were clearly distinguished. Variation of anticytoplasm ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-118
更新日期:1980-06-01 00:00:00
abstract::The attachment of Bradyrhizobium sp. (Lupinus) strain MSDJ718 to excised roots of white lupin was examined. Maximal attachment occurred at early to middle log phases of bacterial growth. This binding was pH dependent, with an optimal value reached at 6.6. Irrespective of the culture age, the attachment was strongly af...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-035
更新日期:1996-03-01 00:00:00
abstract::Uptake of amino acids by Bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. A high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. All amino acids except proline we...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-180
更新日期:1979-10-01 00:00:00
abstract::Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m89-015
更新日期:1989-01-01 00:00:00
abstract::In the past decade, the large tailings pond (Mildred Lake Settling Basin) on the Syncrude Canada Ltd. lease near Fort McMurray, Alta., has gone methanogenic. Currently, about 60%-80% of the flux of gas across the surface of the tailings pond is methane. As well as adding to greenhouse gas emissions, the production of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with a range of infections, including various lower respiratory infections, otitis media, and conjunctivitis. There is some debate as to whether or not NTHi produces biofilms and, if so, whether or not this is relevant to pathogenesis. A...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2014-0763
更新日期:2015-03-01 00:00:00
abstract::5-Methoxymethyl-2'-deoxyuridine (MMUdR), a drug with potent antiviral activity in vitro against Herpes simplex virus, was investigated for its immunosuppressive effects. Doses as high as 2000mg/kg given daily for 9 days were not immunosupporessive as judged by the fact that treated animals produced normal immune respo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-158
更新日期:1977-08-01 00:00:00
abstract::We examined isolates from 4 commercial bioinsecticides based on different strains of Bacillus thuringiensis subspecies (kurstaki, israelensis, aizawai, and tenebrionis) for the presence of genes encoding proteins with known enterotoxigenicity (nhe, hbl, cytk, ces) and various other putative virulence genes (piplc, sph...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-106
更新日期:2007-12-01 00:00:00
abstract::Addition of Tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. When reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. This floating layer can be obta...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-107
更新日期:1988-05-01 00:00:00
abstract::Two bacterial species, Gram-negative Escherichia coli and Gram-positive Bacillus subtilis, were exposed to different auxins to examine possible effects of these substances on bacterial stress tolerance. Bacterial resistance to UV irradiation, heat shock, and streptomycin was assessed with and without previous exposure...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0266
更新日期:2013-11-01 00:00:00
abstract::Klebsiella pneumoniae was found to effect the reductive dechlorination of methoxychlor (2,2-bis(p-methoxyphenyl)-1, 1, 1-trichloroethane) and its dihydroxy derivative (2, 2-bis (p-hydroxyphenyl)-1, 1, 1-trichloroethane) to dichloro derivatives 2, 2-bis (p-methoxyphenyl)-1, 1-dichloroethane and 2, 2-bis (p-hydroxypheny...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-023
更新日期:1982-02-01 00:00:00
abstract::Virulent strains of the fish pathogen Aeromonas salmonicida, which have surface S-layers (S+), efficiently adhere to, enter, and survive within macrophages. Here we report that S+ bacteria were 10- to 20-fold more adherent to non-phagocytic fish cell lines than S-layer-negative (S-) mutants. When reconstituted with ex...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-034
更新日期:2000-07-01 00:00:00
abstract::A mesophilic anaerobe, a member of the Bacteroidaceae family (NRC2248), isolated from a cellulose-enrichment culture, digested untreated Whatman cellulose powder and HCl-treated cotton battings while producing hydrogen, carbon dioxide, cellobiose, glucose, and acetic acid as the sole volatile acid. This organism also ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-222
更新日期:1979-12-01 00:00:00
abstract::BaeR, a response regulator protein, takes part in multidrug efflux, bacterial virulence activity, and other biological functions. Recently, BaeR was shown to induce inflammatory responses by activating the mitogen-activated protein kinases (MAPKs). In this study, we investigated additional pathways used by BaeR to ind...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2016-0057
更新日期:2016-09-01 00:00:00
abstract::The susceptibility of Cephalosporium acremonium to selected amino acid analogues was markedly influenced by the carbon source used in the test media. Lysine hydroxamate, beta-hydroxy norvaline, and hexafluorovaline were toxic when tested with ribose, ribose or fructose, and ribose or galactose, respectively. In contra...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-120
更新日期:1979-07-01 00:00:00
abstract::Lysis of mid-log phase cells of the Gram-positive bacterium, Corynebacterium sepedonicum, by a combination of lysozyme treatment and incubation with alkaline sodium dodecyl sulfate at 56 degrees C led to the recovery of a single plasmid. The plasmid was purified in CsCl density gradients, and its molecular weight esti...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-116
更新日期:1986-08-01 00:00:00
abstract::The suitability of two types of membrane filters for scanning and transmission electron-microscopical examination of chlamydospores formed from macroconidia of Fusarium solani from soil was tested. An improved method to incubate propagules in soil and to collect them free from soil particles for electron-microscopical...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-239
更新日期:1976-11-01 00:00:00
abstract::Several probes for measuring dissolved carbon dioxide (CO2) concentration were installed in a 68-litre fermentor and their effectiveness compared. Submerged silastic rubber tubing gave reproducible results over a wide range of operating conditions and was generally superior to all other probes evaluated. The silastic ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-007
更新日期:1976-01-01 00:00:00
abstract::Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal comple...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-085
更新日期:2005-07-01 00:00:00
abstract::Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and th...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-236
更新日期:1978-12-01 00:00:00
abstract::The use of the food-grade bacterium Lactococcus lactis as a new cell factory is a promising alternative expression system for producing a desired protein. The Omp16-IL2 fusion protein antigen was cloned, expressed, and purified in this study. The Omp16-IL2 fusion gene was designed and cloned in pGH plasmid with approp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0261
更新日期:2020-01-01 00:00:00
abstract::The metal-binding properties of Pseudomonas aeruginosa PAO1 biofilms were investigated using four metals (Cu, Fe, Au, and La). All but one of the metals (i.e., Cu) were bound by the biofilms in amounts that were significantly greater than those bound by planktonically grown cells of the same strain. Lanthanum precipit...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::The expression of the toxA and regA genes in Pseudomonas aeruginosa is negatively regulated by iron at the transcriptional level. We have previously described ptxR, an exotoxin A regulatory gene which appears to enhance toxA expression through regA. In this study, we have tried to determine if ptxR expression correlat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Scanning and transmission electron microscopy was applied for a morphological study of three strains of Bifidobacterium grown on solid or liquid media. The pronounced pleomorphism of the cultures previously observed by light microscopy was confirmed. A possible sequence of the morphological events during transformatio...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-197
更新日期:1975-09-01 00:00:00
abstract::The presence of other soil microorganisms might influence the ability of rhizobacterial inoculants to promote plant growth either by reducing contact between the inoculant and the plant root or by interfering with the mechanism(s) involved in rhizobacterially mediated growth promotion. We conducted the following exper...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-080
更新日期:2001-09-01 00:00:00
abstract::One of the proposed mechanisms by which rhizobacteria enhance plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin riboside (DHZR) in pure culture. Cytokinin production by Pseudomonas fluorescens G20-18, a rifa...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-029
更新日期:2001-05-01 00:00:00
abstract::The gene-encoding HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus JCM2501 was amplified and expressed in Escherichia coli and the enzymatic features were examined. The enzyme was detected mainly in the membrane fraction. It catalyzed the sulfide-dependent menaquinone (MK) reduction showing special enz...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-077
更新日期:2007-09-01 00:00:00
abstract::DNA hybridization studies were conducted to determine the taxonomic status of the aquatic group of Metschnikowia species and their varieties. Among the DNAs of the four varieties of Metschnikowia bicuspidata, that of Metschnikowia bicuspidata var. australis showed 37 to 51% relative binding with the DNAs of Metschniko...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m85-170
更新日期:1985-10-01 00:00:00