Abstract：
:Porcine epidemic diarrhea virus (PEDV) causes severe infectious diseases in all ages of swine and leads to serious economic losses. Serologic tests are widely accepted and used to detect anti-PEDV antibodies that could indicate PEDV infection or vaccination. In this study, PEDV recombinant S1 protein (rS1) was expressed with the Bac-to-Bac system and purified by nickel-affinity chromatography. An indirect enzyme-linked immunosorbent assay based on rS1 (rS1-ELISA) was then developed and optimized by checkerboard assays with serial dilutions of antigen and serum. Serum samples from 453 domestic pigs and 42 vaccinated pigs were analyzed by the indirect fluorescent antibody (IFA) test and rS1-ELISA. Taking IFA as a gold standard, rS1-ELISA produced a high sensitivity (90.7%) and specificity (94.6%) by a receiver operating characteristic (ROC) curve. In addition, ROC analysis also revealed that rS1-ELISA was consistent with IFA (area under the curve 0.9583 ± 0.0082). This rS1-ELISA was then applied to antibody detection in inactivated PEDV vaccinated pigs. The antibody could be detected 2-4 weeks after the first inoculation. These results indicated that the rS1-ELISA established in this study provides a promising and reliable tool for serologic detection of anti-PEDV IgG antibodies in infected or vaccinated pigs.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Shan Y,Liu Y,Liu Z,Li G,Chen C,Luo H,Chen Y,Guo N,Shi X,Zhang X,Fang W,Li Xdoi
10.1139/cjm-2018-0240subject
Has Abstractpub_date
2019-05-01 00:00:00pages
343-352issue
5eissn
0008-4166issn
1480-3275journal_volume
65pub_type
杂志文章abstract：:Antagonistic activity of the bacterium Pseudomonas cepacia against Trichoderma viride was greatly influenced by nutritional and environmental conditions. Xylose and trehalose strongly enhanced the antifungal activity of P. cepacia, whereas mannitol and glucose had little effect. The carbon sources that enhanced the an...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-152
更新日期：1991-11-01 00:00:00
abstract：:Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-038
更新日期：2004-08-01 00:00:00
abstract：:Grapes inoculated with poliovirus 1 and coxsackievirus B5 were washed with water, 0.5% polyehtylene glycol, or phosphate-buffered saline with 1% serum. These washes were equally efficient at removing virus but much of the virus in the water was noninfectious until treated with 0.5% polyethylene glycol. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-195
更新日期：1977-09-01 00:00:00
abstract：:To isolate thiamine excretors and (or) overproducers, 188 cultures belonging to nine yeast and three fungal genera were screened. Nine excreted thiamine as determined by both the presence of cross-feeding zones on a thiamine-free agar medium seeded with a thiamine-requiring yeast strain and by the direct detection of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-189
更新日期：1992-11-01 00:00:00
abstract：:The attachment of Bradyrhizobium sp. (Lupinus) strain MSDJ718 to excised roots of white lupin was examined. Maximal attachment occurred at early to middle log phases of bacterial growth. This binding was pH dependent, with an optimal value reached at 6.6. Irrespective of the culture age, the attachment was strongly af...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-035
更新日期：1996-03-01 00:00:00
abstract：:Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-070
更新日期：1996-05-01 00:00:00
abstract：:Of 24 bacterial species examined for lisinopril refractive peptidyl dipeptidase activity, only 8 contained activity. Activity in Pseudomonas maltophilia was more than fourfold higher than that of any other species. Pseudomonas maltophilia may be unique among bacteria in possessing high peptidyl dipeptidase activity th...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-010
更新日期：1990-01-01 00:00:00
abstract：:The B subunit of Escherichia coli heat-labile enterotoxin (LTB), a nontoxic molecule with potent biological properties, is a powerful mucosal and parenteral adjuvant that induces a strong immune response against co-administered or coupled antigens. In this paper, the effect of LTB on the humoral immune response to rec...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-056
更新日期：2008-08-01 00:00:00
abstract：:Seventeen of 22 group A meningococcal strains were separated into four serotypes by a microbactericidal assay using group A antisera. In addition, 6 of 19 of these strains were killed by a group B antiserum and similar cidal patterns were obtained with a group C antiserum. There was little correlation between type and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-139
更新日期：1979-08-01 00:00:00
abstract：:The bacterial flora of an experimental plant that processes liquid swine manure by an aerated compartmented (multistage) lagoon system has been studied. The total flora is characterized by a larger number of oligotrophic bacteria than eutrophic ones. Each compartment displays a specific flora, different from the flora...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-154
更新日期：1997-11-01 00:00:00
abstract：:Fluorescein isothiocyanate-conjugated antibody to purified enterotoxin of Clostridium perfringens was used to study the intracellular formation of enterotoxin by this organism. Enterotoxin was detected at 4 h of growth at the end of the cell containing forespore. With the development of the spore, enterotoxin accumula...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-134
更新日期：1977-07-01 00:00:00
abstract：:Ribosomal preparations from Neisseria gonorrhoeae types 1 and 4 were examined for their in vitro stimulation of mouse splenocytes to determine the ribosomal moiety or contaminant responsible for the immunoproliferative activity. In immunodiffusion tests with homologous rabbit antiserum, crude 70S ribosomes formed four...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-094
更新日期：1978-05-01 00:00:00
abstract：:We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in a dctA gene. Levels of dctA gene expression ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-008
更新日期：1992-01-01 00:00:00
abstract：:DNA-degrading activity from anaerobic samples of bovine ruminal fluid, primary anaerobic digestor wastewater, freshwater sediments, and marine sediments was observed in the presence of 5 mM EDTA. Nuclease activity experiments involved exposing salmon chromosomal DNA to the environmental samples in 50 mM pH 7.2 buffer,...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-08-01 00:00:00
abstract：:Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent micr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-063
更新日期：1979-03-01 00:00:00
abstract：:HPr is a protein of the phosphoenolpyruvate:sugar phosphotransferase transport system (PTS). In gram-positive bacteria, HPr can be phosphorylated on Ser-46 by the kinase/phosphorylase HprK/P and on His-15 by phospho-enzyme I (EI~P) of the PTS. In vitro studies with purified HPrs from Bacillus subtilis, Enterococcus fa...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-085
更新日期：2008-11-01 00:00:00
abstract：:The structures of lipopolysaccharides (LPSs) of lic1 and lic1/lic2 mutants from Haemophilus influenzae type b strain Eagan (RM153) were investigated using methylation analysis, electrospray ionization - mass spectrometry, and nuclear magnetic resonance spectroscopy on O-deacylated, O- and N-deacylated core oligosaccha...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w08-009
更新日期：2008-04-01 00:00:00
abstract：:Unicellular green alga represents not only a convenient model for its biochemical and physiological studies but also a sensitive system to test the effects of various environmental factors. Algae cells of two strains, SA-3 strain (exsymbiotic from Paramecium bursaria) and Chlorella vulgaris c-27, were asynchronously c...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-143
更新日期：2002-02-01 00:00:00
abstract：:To determine the ability of environmental bacteria to metabolize the frequently prescribed fluoroquinolone drug ciprofloxacin, eight Mycobacterium spp. cultures were grown for 4 days in a medium containing sorbitol and yeast extract with 100 mg x L(-1) ciprofloxacin. After the cultures had been centrifuged and the sup...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-101
更新日期：2007-01-01 00:00:00
abstract：:The new United States Drinking Water Regulations state that water systems must analyze for Escherichia coli or fecal coliforms on any routine or repeat sample that is positive for total coliforms. The proposed methods for the detection of E. coli are based on beta-glucuronidase activity, using the fluorogenic substrat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-157
更新日期：1991-12-01 00:00:00
abstract：:Hydrogen cyanide production by Pseudomonas aeruginosa growing in a synthetic medium required aerobosis but operated efficiently at low dissolved oxygen concentration. Half maximum levels of cyanogenesis occurred at 0.015 microM oxygen; maximum cyanogenesis occurred over a wide range, 0.1-180 microM, of oxygen concentr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-209
更新日期：1983-10-01 00:00:00
abstract：:Cellobiose transport by the cellulolytic ruminal anaerobe Fibrobacter (Bacteroides) succinogenes was measured using randomly tritiated cellobiose. When assayed at the same concentration (1 mM), total cellobiose uptake was one-fourth to one-third that of total glucose uptake. The abilities of F. succinogenes to transpo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-021
更新日期：1991-02-01 00:00:00
abstract：:The possibility of using the volatile-producing fungus Muscodor albus for biofumigation against building molds was investigated. Several species of Aspergillus and Penicillium as well as fungi belonging to nine other genera were inhibited or killed in vitro by volatiles produced by potato dextrose agar or rye grain cu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W06-124
更新日期：2007-03-01 00:00:00
abstract：:Screening for mycotoxin production by Chaetomium spp. and related fungi on rice culture was conducted by a combination of cytotoxicity tests using HeLa cells and thin-layer chromatography. Producers of sterigmatocystin, O-methylsterigmatocystin, chaetochromin, chaetocin, chetomin, cochliodinols, and mollicellin G were...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-119
更新日期：1981-08-01 00:00:00
abstract：:Overexpression and purification are procedures used to allow functional and structural characterization of proteins. Many overexpressed proteins are partially or completely insoluble, and can not be easily purified. The NifA protein is an enhancer-binding protein involved in activating the expression of nif and some f...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w00-094
更新日期：2000-11-01 00:00:00
abstract：:Sixty-four species of filamentous fungi from five flare pits in northern and western Canada were tested for their ability to degrade crude oil using gas chromatographic analysis of residual hydrocarbons following incubation. Nine isolates were tested further using radiorespirometry to determine the extent of mineraliz...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an indigenous infection with Escherichia coli (K. Nomoto, T. Yokokura, Y. Yoshikai, et al. Can. J. Microbiol. 37:244-247, 1991). In the present study, we demonstrate that nonspecific immunostimulation...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-126
更新日期：1992-08-01 00:00:00
abstract：:Leptospira interrogans serotype pomona was isolated from the kidneys of a normal striped skunk (Mephitis mephitis hudsonicus) collected near Kindersley, Saskatchewan. Although there were no gross lesions, small foci of chronic interstitial nephritis were observed microscopically. This is the first reported isolation o...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-237
更新日期：1977-12-01 00:00:00
abstract：:Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal comple...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-085
更新日期：2005-07-01 00:00:00
abstract：:Two-dimensional (2-D) polyacrylamide gel electrophoresis was used to detect proteins induced in Frankia sp. strain ACN14a-tsr by root exudates of its symbiotic host, Alnus glutinosa. The 5 most prominent proteins were purified from 2-D gels and characterized by N-terminal sequencing. All of these proteins had a high p...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-046
更新日期：2001-06-01 00:00:00
