Induction and characterization of morphologic mutants in a natural Saccharomyces cerevisiae strain.

Abstract:

:Saccharomyces cerevisiae is a good model with which to study the effects of morphologic differentiation on the ecological behaviour of fungi. In this work, 33 morphologic mutants of a natural strain of S. cerevisiae, obtained with UV mutagenesis, were selected for their streak shape and cell shape on rich medium. Two of them, showing both high sporulation proficiency and constitutive pseudohyphal growth, were analysed from a genetic and physiologic point of view. Each mutant carries a recessive monogenic mutation, and the two mutations reside in unlinked genes. Flocculation ability and responsiveness to different stimuli distinguished the two mutants. Growth at 37 degrees C affected the cell but not the colony morphology, suggesting that these two phenotypes are regulated differently. The effect of ethidium bromide, which affects mitochondrial DNA replication, suggested a possible "retrograde action" of mitochondria in pseudohyphal growth.

journal_name

Can J Microbiol

authors

Barberio C,Bianchi L,Pinzauti F,Lodi T,Ferrero I,Polsinelli M,Casalone E

doi

10.1139/W06-132

subject

Has Abstract

pub_date

2007-02-01 00:00:00

pages

223-30

issue

2

eissn

0008-4166

issn

1480-3275

pii

w06-132

journal_volume

53

pub_type

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