Induction of resistance in Mycobacterium smegmatis.

abstract：:Eighteen gram-negative marine bacteria and two terrestrial species, Escherichia coli and Pseudomonas aeruginosa, were examined for their sensitivity to lysis in distilled water after exposure to a salt solution containing a sea water concentration of Mg2+ (0.05 M) or to 0.5 M NaCl. A spectrum of lytic susceptibility w...

journal_title：Canadian journal of microbiology

authors： Laddaga RA,MacLeod RA

更新日期：1982-04-01 00:00:00

abstract：:Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with a range of infections, including various lower respiratory infections, otitis media, and conjunctivitis. There is some debate as to whether or not NTHi produces biofilms and, if so, whether or not this is relevant to pathogenesis. A...

journal_title：Canadian journal of microbiology

authors： Obaid NA,Jacobson GA,Tristram S

更新日期：2015-03-01 00:00:00

abstract：:Bacillus stearothermophilus NCA 2184 lost viability and subsequently released cytoplasmic components when suspended in 0.1 M tris(hydroxymethyl)aminomethane (Tris) buffer (pH 7.2) and incubated at 60 degrees C. Cell lysis was prevented by the addition of 10 mM CaCl2 to the Tris-buffer suspension. Cells which were incu...

journal_title：Canadian journal of microbiology

authors： Mosley GA,Card GL,Koostra WL

更新日期：1976-04-01 00:00:00

abstract：:Approximately 20% of Canada's oil supply now comes from the extraction of bitumen from the oil sands deposits in northeastern Alberta. The oil sands are strip-mined, and the bitumen is typically separated from sand and clays by an alkaline hot water extraction process. The rapidly expanding oil sands industry has mill...

journal_title：Canadian journal of microbiology

authors： Fedorak PM,Coy DL,Salloum MJ,Dudas MJ

更新日期：2002-01-01 00:00:00

abstract：:We compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (Helicoverpa armigera), using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences and 16S library sequence analysis. DGGE profiles and 16S rDNA library se...

journal_title：Canadian journal of microbiology

authors： Xiang H,Wei GF,Jia S,Huang J,Miao XX,Zhou Z,Zhao LP,Huang YP

更新日期：2006-11-01 00:00:00

abstract：:Overexpression and purification are procedures used to allow functional and structural characterization of proteins. Many overexpressed proteins are partially or completely insoluble, and can not be easily purified. The NifA protein is an enhancer-binding protein involved in activating the expression of nif and some f...

journal_title：Canadian journal of microbiology

authors： Monteiro RA,Souza EM,Yates MG,Pedrosa FO,Chubatsu LS

更新日期：2000-11-01 00:00:00

abstract：:Eight murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin D (SED) were obtained by fusion of myeloma cells with mouse spleen cells immunized with SED only or a combination of SED and either enterotoxin A (SEA) or enterotoxin E (SEE). When only SED was used as an immunogen, six MAbs were specific for...

journal_title：Canadian journal of microbiology

authors： Shinagawa K,Omoe K,Matsusaka N,Sugii S

更新日期：1991-08-01 00:00:00

abstract：:The tuberculin activity of protoplasmic extracts isolated from living BCG was purified successively by gel filtration on Sephadex G-100 and G-75, and by electrophoresis on 7.5% and on gradient (6-18%) polyacrylamide gels. The tuberculin-active fractions, as determined in BCG-sensitized guinea pigs, were used as the st...

journal_title：Canadian journal of microbiology

authors： Laguerre M,Turcotte R

更新日期：1975-12-01 00:00:00

abstract：:The Bacillus subtilis glutamyl-tRNA synthetase (GluRS), encoded by the gltX gene, aminoacylates its homologous tRNA(Glu) and tRNA(Gln) with glutamate. This gene was cloned with its sigma A promoter and a downstream region including a rho-independent terminator in the shuttle vector pRB394 for Escherichia coli and B. s...

journal_title：Canadian journal of microbiology

authors： Pelchat M,Lacoste L,Yang F,Lapointe J

更新日期：1998-04-01 00:00:00

abstract：:The study of diaminopimelic acid (DAP) incorporation and turnover during growth recovery in chloramphenicol-treated (CMP-treated) Bacillus megaterium cells showed that two kinds of turnover occurred. A low acid-soluble turnover appeared as soon as growth resumed in bacteria labeled before the CMP treatment and at the ...

journal_title：Canadian journal of microbiology

authors： Fréhel C,de Chastellier C,Ryter A

更新日期：1980-03-01 00:00:00

abstract：:Vaccination is the most promising strategy to reduce the incidence of pneumococcal infection. Although there are vaccines available, all of them are based on polysaccharide antigens (conjugated or not). In addition to their high cost, those vaccines do not cover all serotypes. To overcome these hindrances, we evaluate...

journal_title：Canadian journal of microbiology

authors： Araújo HF,Campos PC,Camargo DR,Pereira FN,Samuel ML,Oliveira MA,Fortes-Dias CL,Leclercq SY

更新日期：2012-09-01 00:00:00

abstract：:Eighteen yeast species of the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus, each one represented by its type strain, were investigated with the objective of evaluating their carotenoid composition. The pigments were extracted from yeast cells, quantified by high pressure liquid chromatography ...

journal_title：Canadian journal of microbiology

authors： Buzzini P,Innocenti M,Turchetti B,Libkind D,van Broock M,Mulinacci N

更新日期：2007-08-01 00:00:00

abstract：:The bacterial flora of an experimental plant that processes liquid swine manure by an aerated compartmented (multistage) lagoon system has been studied. The total flora is characterized by a larger number of oligotrophic bacteria than eutrophic ones. Each compartment displays a specific flora, different from the flora...

journal_title：Canadian journal of microbiology

authors： Chikh G,Pourquié J,Kaiser P,Davila AM

更新日期：1997-11-01 00:00:00

abstract：:The gene-encoding HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus JCM2501 was amplified and expressed in Escherichia coli and the enzymatic features were examined. The enzyme was detected mainly in the membrane fraction. It catalyzed the sulfide-dependent menaquinone (MK) reduction showing special enz...

journal_title：Canadian journal of microbiology

authors： Shibata H,Suzuki K,Kobayashi S

更新日期：2007-09-01 00:00:00

abstract：:The effect of the transition metal compound trans-[Rh(4-ethylpyridine)4Cl2]Cl x 2H2O on the syntheses of DNA, RNA, and protein has been investigated for an auxotrophic bacterial strain, Escherichia coli JS-1, incapable of thymidine, uridine, and histidine syntheses. At low concentration (7.4 x 10(-6) M), this rhodium ...

journal_title：Canadian journal of microbiology

authors： Seeman SL,Jack TR

更新日期：1980-05-01 00:00:00

abstract：:Degradation of purified rubella virus by heat treatment (37, 45, or 56 degrees C) revealed the following structures. The viral envelope, a modified cellular membrane, bears spherical subunits, 5-6 nm in diameter, hexamers, or pentamers. Two glycoproteins, VP-2 (50 000 daltons) and VP-3 (63 000 daltons), are associated...

journal_title：Canadian journal of microbiology

authors： Payment P,Ajdukovic D,Pavilanis V

更新日期：1975-05-01 00:00:00

abstract：:The effects of various hydrocarbon substrates, and a chemical surfactant capable of enhancing crude-oil biodegradation, on the community structure of a mixed-bacterial inoculum were examined in batch culture. Of 1000 TSA-culturable isolates, 68.6% were identified at the genus level or better by phospholipid fatty acid...

journal_title：Canadian journal of microbiology

authors： Van Hamme JD,Odumeru JA,Ward OP

更新日期：2000-05-01 00:00:00

abstract：:Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...

journal_title：Canadian journal of microbiology

authors： Figueira MM,Ciminelli VS,de Andrade MC,Linardi VR

更新日期：1996-05-01 00:00:00

abstract：:Neisseria gonorrhoeae exist in transparent (Tr) and opaque (Op) colony forms. Op forms are recovered from patients early in the menstrual cycle; Tr colonies predominate late in the cycle. The mechanism for this colonial variation was examined by determining the influence of gonodal hormones on growth inhibition of Op ...

journal_title：Canadian journal of microbiology

authors： Salit IE

更新日期：1982-03-01 00:00:00

abstract：:In Escherichia coli, the N-terminal domain of the essential protein FtsK (FtsKN) is proposed to modulate septum formation through the formation of dynamic and essential protein interactions with both the Z-ring and late-stage division machinery. Using genomic mutagenesis, complementation analysis, and in vitro pull-do...

journal_title：Canadian journal of microbiology

authors： Berezuk AM,Roach EJ,Seidel L,Lo RY,Khursigara CM

更新日期：2020-04-01 00:00:00

abstract：:The addition of various concentrations of monensin (1,5, and 10 micrograms/mL) to the culture medium inhibits the fungal growth and perturbs exopolysaccharides secretion, provoking a decrease of production in Botrytis cinerea and an increase in Sclerotium rolfsii. The ionophore induces also modifications in both polym...

journal_title：Canadian journal of microbiology

authors： Koulali Y,Talouizte A,Fonvieille JL,Dargent R

更新日期：1996-09-01 00:00:00

abstract：:DNA hybridization studies were conducted to determine the taxonomic status of the aquatic group of Metschnikowia species and their varieties. Among the DNAs of the four varieties of Metschnikowia bicuspidata, that of Metschnikowia bicuspidata var. australis showed 37 to 51% relative binding with the DNAs of Metschniko...

journal_title：Canadian journal of microbiology

authors： Mendonça-Hagler LC,Hagler AN,Phaff HJ,Tredick J

更新日期：1985-10-01 00:00:00

abstract：:Southern bean mosaic virus (SBMV) protein was cleaved with cyanogen bromide and a highly basic peptide, CB-1, was isolated by ion exclusion and ion-exchange chromatography. Twelve peptides were separated from a tryptic digest of CB-1 by ion-exchange chromatography and the composition of these peptides was similar to t...

journal_title：Canadian journal of microbiology

authors： Tremaine JH,Ronald WP,Kelly EM

更新日期：1980-12-01 00:00:00

abstract：:The use of the food-grade bacterium Lactococcus lactis as a new cell factory is a promising alternative expression system for producing a desired protein. The Omp16-IL2 fusion protein antigen was cloned, expressed, and purified in this study. The Omp16-IL2 fusion gene was designed and cloned in pGH plasmid with approp...

journal_title：Canadian journal of microbiology

authors： Rezaei M,Rabbani Khorasgani M,Zarkesh Esfahani SH,Emamzadeh R,Abtahi H

更新日期：2020-01-01 00:00:00

abstract：:Marine shrimp of the species Penaeus monodon were collected from the coastal region (Haroa) of the deltaic Sundarbans of West Bengal, India during the premonsoon period in 1996. Klebsiella pneumoniae was isolated from the alimentary canal and gills of the shrimp as the sole isolate. All 10 isolated strains were resist...

journal_title：Canadian journal of microbiology

authors： Choudhury P,Kumar R

更新日期：1998-02-01 00:00:00

abstract：:A full-scale nuclear fuel waste disposal container experiment was carried out 240 m below ground in an underground granitic rock research laboratory in Canada. An electric heater was surrounded by buffer material composed of sand and bentonite clay and provided heat equivalent to what is anticipated in a Canadian nucl...

journal_title：Canadian journal of microbiology

authors： Stroes-Gascoyne S,Pedersen K,Haveman SA,Dekeyser K,Arlinger J,Daumas S,Ekendahl S,Hallbeck L,Hamon CJ,Jahromi N,Delaney TL

更新日期：1997-12-01 00:00:00

abstract：:Grapes inoculated with poliovirus 1 and coxsackievirus B5 were washed with water, 0.5% polyehtylene glycol, or phosphate-buffered saline with 1% serum. These washes were equally efficient at removing virus but much of the virus in the water was noninfectious until treated with 0.5% polyethylene glycol. ...

journal_title：Canadian journal of microbiology

authors： Konowalchuk J,Spiers JI

更新日期：1977-09-01 00:00:00

abstract：:Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC 3.1.3.2), cyclic phosphodiesterase, ribonuclease I (EC 3.1.4.22), and phosphoglucose isomerase (EC 5.3.1.9) (PGI is at least par...

journal_title：Canadian journal of microbiology

authors： Chatterjee AK,Ross H,Sanderson KE

更新日期：1976-10-01 00:00:00

abstract：:Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains...

journal_title：Canadian journal of microbiology

authors： Golubev WI,Sampaio JP,Alves L,Golubev NW

更新日期：2004-12-01 00:00:00

abstract：:The in situ degradation of the two nitramine explosives, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), was evaluated using a mixture of RDX and HMX, incubated anaerobically at 10 degrees C with marine sediment from a previous military dumping site of unexplod...

journal_title：Canadian journal of microbiology

authors： Zhao JS,Greer CW,Thiboutot S,Ampleman G,Hawari J

更新日期：2004-02-01 00:00:00