Nutrient uptake by Candida albicans: the influence of cell surface mannoproteins.

Abstract:

:Numerous ultrastructural and biochemical analyses have been performed to characterize the cell wall composition and structure of Candida albicans. However, little investigation has focused on how subtle differences in cell wall structure influence the intracellular transport of amino acids and monosaccharides. In this study C. albicans 4918 and ATCC 10231 were grown in culture conditions capable of modifying surface mannoproteins and induced surface hydrophobic or hydrophilic yeast cell wall states. Subcultures of these hydrophobic and hydrophilic yeasts were subsequently incubated with one of seven L-[3H] amino acids: glycine, leucine, proline, serine, aspartic acid, lysine, or arginine. The transport of [3H] mannose and [3H] N-acetyl-D-glucosamine were also investigated. This study revealed significant strain differences (P < or = 0.05) between hydrophilic and hydrophobic yeast transport of these nutrients throughout a 2 h incubation. Hydrophilic cultures of 4918 and ATCC 10231 transported nearly two times more (pmol mg-1 dry weight) proline, mannose, and N-acetyl-D-glucosamine than hydrophobic yeast. Hydrophobic cultures preferentially incorporated serine and aspartic acid in both these strains. Strain variation was indicated with the transport of leucine, lysine, and arginine, as follows: experiments showed that hydrophilic 4918 cultures selectively transported leucine, lysine, and arginine, whereas, the hydrophobic ATCC 10231 cultures incorporated these amino acids.

journal_name

Can J Microbiol

authors

Braun PC

subject

Has Abstract

pub_date

1999-05-01 00:00:00

pages

353-9

issue

5

eissn

0008-4166

issn

1480-3275

journal_volume

45

pub_type

杂志文章
  • Measurement of dissolved carbon dioxide.

    abstract::Several probes for measuring dissolved carbon dioxide (CO2) concentration were installed in a 68-litre fermentor and their effectiveness compared. Submerged silastic rubber tubing gave reproducible results over a wide range of operating conditions and was generally superior to all other probes evaluated. The silastic ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-007

    authors: Alford JS Jr

    更新日期:1976-01-01 00:00:00

  • Effect of a Vietnamese Cinnamomum cassia essential oil and its major component trans-cinnamaldehyde on the cell viability, membrane integrity, membrane fluidity, and proton motive force of Listeria innocua.

    abstract::The antibacterial mechanism of a Cinnamomum cassia essential oil from Vietnam and of its main component (trans-cinnamaldehyde, 90% (m/m) of C. cassia essential oil) against a Listeria innocua strain was investigated to estimate their potential for food preservation. In the presence of C. cassia essential oil or trans-...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2014-0481

    authors: Trinh NT,Dumas E,Thanh ML,Degraeve P,Ben Amara C,Gharsallaoui A,Oulahal N

    更新日期:2015-04-01 00:00:00

  • A tolerant lactic acid bacteria, Lactobacillus paracasei, and its immunoregulatory function.

    abstract::The aim of the present investigation was to isolate a probiotic strain from 23 samples of yurts cheese and 21 samples of kumiss (collected from scattered households in Xinjiang and Inner Mongolia), and from eN-Lac Capsules, a health-promoting product. The isolates were subjected to biochemical characterization analysi...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2014-0383

    authors: Kou X,Chen Q,Ju X,Liu H,Chen W,Xue Z

    更新日期:2014-11-01 00:00:00

  • A Colletotrichum gloeosporioides cerato-platanin protein, CgCP1, contributes to conidiation and plays roles in the interaction with rubber tree.

    abstract::Colletotrichum gloeosporioides is the causal agent of rubber tree anthracnose and leads to serious losses of natural rubber production. The pathogenesis of C. gloeosporioides on rubber tree remains unknown. Cerato-platanin proteins are small, secreted cysteine-rich proteins that contribute to virulence and function in...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2018-0087

    authors: Wang W,An B,Feng L,He C,Luo H

    更新日期:2018-11-01 00:00:00

  • Concentric bodies in two species of he loculoascomycetes.

    abstract::Concentric bodies were found in vegetative and pycnidial cells of Podoxythium tricothecium and in ascostromal paraphyses of Rhytidhysterium rufulum, two representatives of the Loculoascomycetes. They averages 185 nm in diameter in P. trichothecium and 255 nm in R. rufulum and consisted of three zones. ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m77-218

    authors: Samuelson DA,Bezerra J

    更新日期:1977-10-01 00:00:00

  • Purification and characterization of 2-ethoxyphenol-induced cytochrome P450 from Corynebacterium sp. strain EP1.

    abstract::A soluble cytochrome P450 (P450EP1A) induced by 2-ethoxyphenol was purified to apparent homogeneity from Corynebacterium sp. strain EP1. The P450EP1A showed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight of about 45 kDa. The CO-reduced difference spectra of P450EP1A ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Kawahara N,Ikatsu H,Kawata H,Miyoshi S,Tomochika K,Sinoda S

    更新日期:1999-10-01 00:00:00

  • Antimicrobial susceptibilities and distribution of sequence types of Neisseria gonorrhoeae isolates in Canada: 2010.

    abstract::The monitoring of antimicrobial susceptibilities in Neisseria gonorrhoeae isolates and characterization of N. gonorrhoeae multiantigen sequence types (NG-MAST, ST) provide important surveillance data as resistance rates continue to rise. A total of 2970 N. gonorrhoeae isolates were collected by Canadian provincial pub...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2013-0357

    authors: Martin I,Sawatzky P,Liu G,Allen V,Lefebvre B,Hoang L,Lovgren M,Haldane D,Caeseele PV,Horsman G,Garceau R,Ratnam S,Wong T,Gilmour M

    更新日期:2013-10-01 00:00:00

  • A potential role of Escherichia coli pathobionts in the pathogenesis of pediatric inflammatory bowel disease.

    abstract::Through genomic analysis of mucosa-associated Escherichia coli strains, we found a close genetic association among isolates from pediatric inflammatory bowel disease (IBD) patients. A specific E. coli pathovar, adherent-invasive E. coli (AIEC), was found in Crohn's disease (CD) adult patients - this pathovar has enhan...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w2012-007

    authors: Schippa S,Iebba V,Totino V,Santangelo F,Lepanto M,Alessandri C,Nuti F,Viola F,Di Nardo G,Cucchiara S,Longhi C,Conte MP

    更新日期:2012-04-01 00:00:00

  • Oral administration of Bifidobacterim bifidum for modulating microflora, acid and bile resistance, and physiological indices in mice.

    abstract::Bifidobacteria are generally acknowledged as major gut microflora used as probiotics, which promote human health. In this study, the effects of the administration of Bifidobacterim bifidum on modulating gastrointestinal (GI) tract microflora, acid and bile resistance, and physiological indices in BALB/c mice were inve...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2014-0694

    authors: Wang BG,Xu HB,Wei H,Zeng ZL,Xu F

    更新日期:2015-02-01 00:00:00

  • Test for differentiation of M. tuberculosis and M. bovis from other mycobacteria.

    abstract::A test is described for selective inhibition of Mycobacterium tuberculosis and M. bovis isolates in fluid medium. The method employs rho-nitro-alpha-acetyl-amino-beta-hydroxy-propiophenone (NAP) as an inhibitory agent for differentiation of mammalian tuberculosis strains from other Mycobacteria. ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-124

    authors: Laszlo A,Eidus L

    更新日期:1978-06-01 00:00:00

  • Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta).

    abstract::Uroporphyrinogen decarboxylase (UroD) (EC 4.1.1.37) is an enzyme from the tetrapyrrole biosynthetic pathway, in which chlorophyll is the main final product in algae. This is the first time that a study on UroD activity has been performed in a green alga (Chlorella). We isolated and partially purified the enzyme from a...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W06-117

    authors: Juárez AB,Aldonatti C,Vigna MS,Ríos de Molina Mdel C

    更新日期:2007-02-01 00:00:00

  • Growth and survival of uninjured and sublethally heat-injured Escherichia coli O157:H7 on beef extract medium as influenced by package atmosphere and storage temperature.

    abstract::The effect of atmospheric composition and storage temperature on growth and survival of uninjured and sublethally heat-injured Escherichia coli O157:H7, inoculated onto brain heart infusion agar containing 0.3% beef extract (BEM), was determined. BEM plates were packaged in barrier bags in air, 100% CO2, 100% N2, 20% ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w98-224

    authors: Semanchek JJ,Golden DA,Williams RC

    更新日期:1999-03-01 00:00:00

  • Incidence of Norwalk virus infections during a prospective epidemiological study of drinking water-related gastrointestinal illness.

    abstract::To determine the seroprevalence of Norwalk virus and whether Norwalk virus contributed to an observed increase in illness in tap water drinkers participating in a prospective epidemiological study, sera collected during the study were examined for changes in Norwalk virus antibody titer, using a specific enzyme immuno...

    journal_title:Canadian journal of microbiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1139/m94-128

    authors: Payment P,Franco E,Fout GS

    更新日期:1994-10-01 00:00:00

  • Cultural, morphological, and physiological characteristics of Thermomonospora fusca (strain 190Th).

    abstract::The cultural, morphological, and physiological properties of Thermomonospora fusca (strain 190Th) are described. Its physiological properties show that this species is primarily a carbohydrate-degrading actinomycete which can use a wide range of plant sugars and polymeric carbohydrates as sources of carbon and energy....

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-267

    authors: Crawford DL

    更新日期:1975-11-01 00:00:00

  • The in vitro hydroxylation of 4'-chloro-4-biphenylol by a mushroom tyrosinase preparation.

    abstract::Incubation of 4'-chloro-4-biphenylol with a mushroom tyrosinase preparation gave the catechol, 4'-chloro-3,4-biphenyldiol as the sole in vitro metabolite. This compound was identical with the major rat urinary metabolite of 4'-chloro-4-biphenylol and thus confirms the structure assigned to the metabolite. This result ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-014

    authors: Safe S,Ellis BE,Hutzinger O

    更新日期:1976-01-01 00:00:00

  • Stress resistance of Escherichia coli and Bacillus subtilis is modulated by auxins.

    abstract::Two bacterial species, Gram-negative Escherichia coli and Gram-positive Bacillus subtilis, were exposed to different auxins to examine possible effects of these substances on bacterial stress tolerance. Bacterial resistance to UV irradiation, heat shock, and streptomycin was assessed with and without previous exposure...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2013-0266

    authors: Repar J,Šućurović S,Zahradka K,Zahradka D,Ćurković-Perica M

    更新日期:2013-11-01 00:00:00

  • Sorogen elongation and side branching during fruiting body development in Polysphondylium pallidum.

    abstract::We report a cinematographic analysis of culmination in fruiting bodies of Polysphondylium pallidum. During the final stages of aggregation, sorogen elongation is slow. An increase in the rate of sorogen elongation occurs when the growing stalk contacts the substratum, lifting the cell mass into the air. The elongation...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-163

    authors: O'Day DH,Durston AJ

    更新日期:1980-08-01 00:00:00

  • Biochemical characterization of three mycobacterial ribosomal fractions.

    abstract::The induction of antituberculous immunity by crude ribosomal fractions isolated from Mycobacterium tuberculosis strain H37Ra, M. bovis strain BCG, and M. smegmatis was studied in CF-1 mice. Levels of antituberculous immunity similar to that induced by live BCG were induced by the BCG and H37Ra ribosomal fractions wher...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-032

    authors: Portelance V,Beaudet R

    更新日期:1983-02-01 00:00:00

  • Eight UCA codons differentially affect the expression of the lacZ gene in the divE42 mutant of Escherichia coli.

    abstract::In the divE mutant, which has a temperature-sensitive mutation in the tRNA1(Ser) gene, the synthesis of beta-galactosidase is dramatically decreased at the non-permissive temperature. In Escherichia coli, the UCA codon is only recognized by tRNA1(Ser). Several genes containing UCA codons are normally expressed at 42 d...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Kubo T,Aiso T,Ohki R

    更新日期:2000-06-01 00:00:00

  • Isolation of enterotoxigenic Aeromonas from fish.

    abstract::This paper reports two different enterotoxigenic classes of the genus Aeromonas isolated from fishes. Strains of A. sobria were isolated from healthy fishes only wheras strains of A. hydrophila were isolated from both healthy and moribund fishes. Aeromonas sobria and hydrophilia strains produced a cytotoxic factor and...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m77-174

    authors: Boulanger Y,Lallier R,Cousineau G

    更新日期:1977-09-01 00:00:00

  • Genotyping of Yersinia enterocolitica biotype 1A strains from clinical and nonclinical origins by pulsed-field gel electrophoresis.

    abstract::Yersinia enterocolitica biotype 1A (B1A) strains are considered mainly nonpathogenic. However, some studies considered strains of this biotype to be the causal agents of infections in humans and animals. In South America, there are no studies that have compared clinical and nonclinical strains of B1A typed by pulsed-f...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2014-0211

    authors: Campioni F,Falcão JP

    更新日期:2014-06-01 00:00:00

  • Aerobic adaptation in yeast. I. Changes in metabolic intermediates during a step-down anaerobic-aerobic transfer.

    abstract::Early investigation into the mechanism of mitochondriogenesis in the facultative anaerobe Saccharomyces cerevisiae used anaerobic-aerobic transitions as model systems to study the in vivo assembly of these oxidative organelles. This methodology has become entrenched in the early literature and as a result a definitive...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-126

    authors: Bruver RM,Ball JS,Tustanoff ER

    更新日期:1975-06-01 00:00:00

  • Cytochrome aa3 in facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense.

    abstract::We partially purified and characterized the cytochrome aa3 from the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense. This cytochrome aa3 showed oxygen consumption activity with N, N, N', N'-tetramethyl-1,4-phenylenediamine and ascorbate as substrates, and also displayed bovine cytochrome c o...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w05-040

    authors: Nunoura T,Sako Y,Wakagi T,Uchida A

    更新日期:2005-08-01 00:00:00

  • Sucrose-mediated giant cell formation in the genus Neisseria.

    abstract::Growth of Neisseria perflava, Neisseria cinerea, and Neisseria sicca strain Kirkland in media supplemented with sucrose (0.5 to 5.0% w/v) resulted in the formation of giant cells. Response to sucrose was specific in that a variety of other carbohydrates did not mediate giant cell formation. Giant cells appeared only u...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-065

    authors: Johnson KG,McDonald IJ

    更新日期:1976-03-01 00:00:00

  • [Rubella virus. III. Purification of infected or non-infected Vero cell membranes].

    abstract::Separation of Vero cell membrane in a discontinuous sucrose gradient reveals five fractions. After infection a sixth fraction appears. It contains virioins but mostly modified membranes with subunits 5-6 nm in diameter, probably the hemagglutinin. None of the enzymes used was associated with this fraction. No modifica...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-103

    authors: Payment P,Ajdukovic D,Pavilanis V

    更新日期:1975-05-01 00:00:00

  • Seroepidemiology of Q fever in Nova Scotia and Prince Edward Island.

    abstract::The prevalence of Coxiella burnetti infection (Q fever) was determined among Nova Scotia (N.S.) and Prince Edward Island (P.E.I.) blood donors by using the complement fixation and microimmunofluorescence (IF) test. The complement fixation and IF antibody tests measured antibody prevalence for the phase II or phase I a...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m84-021

    authors: Marrie TJ,Van Buren J,Faulkner RS,Haldane EV,Williams JC,Kwan C

    更新日期:1984-01-01 00:00:00

  • Thymineless death in Escherichia coli mutants deficient in the RecF recombination pathway.

    abstract::Like recF and recQ mutants studied earlier, two other classes of Escherichia coli mutants defective in the RecF conjugal recombination pathway, recJ and recO, were found to be partially resistant to thymineless death. In contrast, a recN mutant, also belonging to the pathway, was indistinguishable from the wild type w...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m88-157

    authors: Nakayama K,Shiota S,Nakayama H

    更新日期:1988-07-01 00:00:00

  • Purification and characterization of a psychrophilic catalase from Antarctic Bacillus.

    abstract::Catalase from Bacillus sp. N2a (BNC) isolated from Antarctic seawater was purified to homogeneity. BNC has a molecular mass of about 230 kDa and is composed of four identical subunits of 56 kDa. The catalase showed optimal activity at 25 degrees C and at a pH range of 6-11. The enzyme could be inhibited by azide, hydr...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w08-066

    authors: Wang W,Sun M,Liu W,Zhang B

    更新日期:2008-10-01 00:00:00

  • Evidence for a relationship between chloroquine and complement from studies with lymphocyte mitogens: possible implications for the mechanism of action of chloroquine in disease.

    abstract::Chloroquine increases the inhibition of cultured lymphocytes by high concentrations of phytohemagglutinin (PHA) or concanavalin A (Con A). The inhibition is also increased by complement. Thus chloroquine and complement have similar effects. Time-course studies show that chloroquine increases the rate of onset of the c...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-231

    authors: Forsdyke DR

    更新日期:1975-10-01 00:00:00

  • Changes in pattern of respiration and glucose utilisation in Candida utilis during the cell cycle: some variations with growth rate.

    abstract::The release of 14CO2 from 14C-labelled glucose(G-1(-14)C, G-3,4(-14)C, G-6(-14)C) was followed in phased cultures of Candida utilis grown in a glucose- mineral salts medium under altered conditions of carbon:nitrogen limitation at doubling times of 2,4 and 6, h. Changes in oxygen uptake and CO2 evolution were observe...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-150

    authors: Dawson PS,Westlake DW

    更新日期:1975-07-01 00:00:00