Abstract：
:The aim of the present investigation was to isolate a probiotic strain from 23 samples of yurts cheese and 21 samples of kumiss (collected from scattered households in Xinjiang and Inner Mongolia), and from eN-Lac Capsules, a health-promoting product. The isolates were subjected to biochemical characterization analysis and were tested for tolerance to low pH, sodium salt, bile salt, pepsin, and trypsin. 16S DNA sequence analysis was conducted to identify the strain. The possible dose-dependent role of strain LP2 in immunomodulation was investigated using the ICR mouse model (from the Institute of Cancer Research). Daily, we conducted clinical observations, a carbon clearance test, a spleen lymphocyte proliferation test, and measurements of body mass and lymphoid organ index. Natural killer cell activity and delayed-type hypersensitivity reaction were determined. The results showed that 3 selected strains (LP2, LP4, and LP9) had high tolerance to low pH, sodium chloride, and bile salt and were not significantly different from Lactobacillus paracasei in terms of morphology, colony, and biochemistry characterizations. A further tolerance test showed that LP2 had the highest survival rate (90%) under the conditions of pH 3.0, 0.3% bile salt, 10 mg/mL pepsin, and 10 mg/mL trypsin for 24 h. The sequence heterogeneities within the 16S rDNA genes molecularly elucidated that the LP2 belongs to the L. paracasei family, on the basis of a homology of 99.6%. A significant enhanced footpad swelling reaction and natural killer cell activity in the middle-dose (10(8) cfu/mL) and the high-dose (10(9) cfu/mL) groups were observed but without obvious dose dependence (P < 0.05). Lymphocyte proliferation was also increased significantly in a dose-dependent manner (P < 0.01) compared with that of the control group, indicating a positive immunoregulatory effect.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Kou X,Chen Q,Ju X,Liu H,Chen W,Xue Zdoi
10.1139/cjm-2014-0383subject
Has Abstractpub_date
2014-11-01 00:00:00pages
729-36issue
11eissn
0008-4166issn
1480-3275journal_volume
60pub_type
杂志文章abstract：:Pyrenocine A, a phytotoxin produced by Pyrenochaeta terrestris (Hansen) Gorenz, Walker and Larson, possesses general antibiotic activity against plants, fungi, and bacteria. Effective doses for 50% inhibition (ED50s) are 4 micrograms/mL for onion seedling elongation; 14, 20, 20, and 25 micrograms/mL for the germinatio...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-055
更新日期：1987-04-01 00:00:00
abstract：:A bacterium C1010, isolated from the rhizospheres of cucumbers in fields in Korea, degraded the microbial quorum-sensing molecules, hexanoyl homoserine lactone (HHSL), and octadecanoyl homoserine lactone (OHSL). Morphological characteristics and 16S rRNA sequence analysis identified C1010 as Acinetobacter sp. strain C...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-083
更新日期：2004-11-01 00:00:00
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journal_title：Canadian journal of microbiology
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doi：10.1139/m78-039
更新日期：1978-03-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-031
更新日期：1996-02-01 00:00:00
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journal_title：Canadian journal of microbiology
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doi：10.1139/W07-002
更新日期：2007-04-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0481
更新日期：2015-04-01 00:00:00
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journal_title：Canadian journal of microbiology
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doi：10.1139/w03-098
更新日期：2003-12-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m87-139
更新日期：1987-09-01 00:00:00
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journal_title：Canadian journal of microbiology
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doi：10.1139/cjm-2019-0261
更新日期：2020-01-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-018
更新日期：1975-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-052
更新日期：1981-03-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-064
更新日期：1976-03-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W07-036
更新日期：2007-06-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-220
更新日期：1983-10-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-129
更新日期：2006-04-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w07-116
更新日期：2008-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-199
更新日期：1981-12-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-174
更新日期：1977-09-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-178
更新日期：1980-09-01 00:00:00
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-188
更新日期：1979-10-01 00:00:00
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journal_title：Canadian journal of microbiology
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更新日期：2003-11-01 00:00:00
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journal_title：Canadian journal of microbiology
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更新日期：2011-08-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-022
更新日期：1978-02-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2001-08-01 00:00:00
abstract：:Environmentally compatible control measures are needed for suppression of Phytophthora capsici on pepper. Twenty-three isolates of Trichoderma were screened for suppression of a mixture of 4 genetically distinct isolates of this pathogen on bell pepper (Capsicum anuum) in greenhouse pot assays. Of these 23 isolates, G...
journal_title：Canadian journal of microbiology
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更新日期：2010-10-01 00:00:00
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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更新日期：1979-03-01 00:00:00
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journal_title：Canadian journal of microbiology
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journal_title：Canadian journal of microbiology
pub_type： 杂志文章
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更新日期：2001-05-01 00:00:00
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journal_title：Canadian journal of microbiology
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更新日期：2009-05-01 00:00:00
