Identification of fecal contamination sources in water using host-associated markers.

Abstract:

:In British Columbia, Canada, drinking water is tested for total coliforms and Escherichia coli, but there is currently no routine follow-up testing to investigate fecal contamination sources in samples that test positive for indicator bacteria. Reliable microbial source tracking (MST) tools to rapidly test water samples for multiple fecal contamination markers simultaneously are currently lacking. The objectives of this study were (i) to develop a qualitative MST tool to identify fecal contamination from different host groups, and (ii) to evaluate the MST tool using water samples with evidence of fecal contamination. Singleplex and multiplex polymerase chain reaction (PCR) were used to test (i) water from polluted sites and (ii) raw and drinking water samples for presence of bacterial genetic markers associated with feces from humans, cattle, seagulls, pigs, chickens, and geese. The multiplex MST assay correctly identified suspected contamination sources in contaminated waterways, demonstrating that this test may have utility for heavily contaminated sites. Most raw and drinking water samples analyzed using singleplex PCR contained at least one host-associated marker. Singleplex PCR was capable of detecting host-associated markers in small sample volumes and is therefore a promising tool to further analyze water samples submitted for routine testing and provide information useful for water quality management.

journal_name

Can J Microbiol

authors

Krentz CA,Prystajecky N,Isaac-Renton J

doi

10.1139/cjm-2012-0618

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

210-20

issue

3

eissn

0008-4166

issn

1480-3275

journal_volume

59

pub_type

杂志文章
  • Ecology of cultivable yeasts in pristine forests in northern Patagonia (Argentina) influenced by different environmental factors.

    abstract::Environmental factors influencing the occurrence and community structure of soil yeasts in forests are not well studied. There are few studies dedicated to Southern Hemisphere soil yeasts populations and even fewer focused on temperate forests influenced by volcanic activity. The present work aimed to study the ecolog...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2013-0897

    authors: Mestre MC,Fontenla S,Rosa CA

    更新日期:2014-06-01 00:00:00

  • Survival in foods of Staphylococcus aureus grown under optimal and stressed conditions and the effect of some food preservatives.

    abstract::Staphylococcus aureus was grown in a rich peptone medium which became alkaline with continued incubation. Cells were grown at 37 degrees C and in the same medium containing 1 M NaCl at 46 degrees C, a temperature at which this organism can grow only when protected by NaCl. Cells of these cultures are hereafter called ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-049

    authors: El-Banna AA,Hurst A

    更新日期:1983-03-01 00:00:00

  • Mapping of transfer and H pilus coding regions of the IncHII plasmid pHH1508a.

    abstract::The IncHII plasmid pHH1508a (208 kilobases) encodes resistance to potassium tellurite, trimethoprim, and streptomycin. Conjugative pili encoded by pHH1508a were isolated, purified, and used for preparation of anti-H pilus antiserum. Immuno-gold labelling experiments using H pilus specific antiserum showed that antigen...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m89-043

    authors: Yan W,Taylor DE

    更新日期:1989-02-01 00:00:00

  • Relationship between clinical site of isolation and ability to form biofilms in vitro in nontypeable Haemophilus influenzae.

    abstract::Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with a range of infections, including various lower respiratory infections, otitis media, and conjunctivitis. There is some debate as to whether or not NTHi produces biofilms and, if so, whether or not this is relevant to pathogenesis. A...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2014-0763

    authors: Obaid NA,Jacobson GA,Tristram S

    更新日期:2015-03-01 00:00:00

  • Chemical and enzymatic changes in the cell walls of Candida albicans and Saccharomyces cerevisiae by scanning electron microscopy.

    abstract::Candida albicans and Saccharomyces cerevisiae cells were examined by scanning scanning electron microscopy before and after extraction of the mannans of the cell wall. The surfaces of control cells were smooth; after mannan extraction they were rough and showed erosions which were particularly striking within the area...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m80-164

    authors: Koch Y,Rademacher KH

    更新日期:1980-08-01 00:00:00

  • Physical, chemical, and microbiological characteristics of microbial mats (kopara) in the South Pacific atolls of French Polynesia.

    abstract::Microbial mats that develop in shallow brackish and hyposaline ponds in the rims of two French polynesian atolls (Rangiroa and Tetiaroa) were intensively investigated during the past three years. Comparative assessment of these mats (called kopara in polynesian language) showed remarkable similarities in their composi...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:

    authors: Che LM,Andréfouët S,Bothorel V,Guezennec M,Rougeaux H,Guezennec J,Deslandes E,Trichet J,Matheron R,Le Campion T,Payri C,Caumette P

    更新日期:2001-11-01 00:00:00

  • [Rubella virus. III. Purification of infected or non-infected Vero cell membranes].

    abstract::Separation of Vero cell membrane in a discontinuous sucrose gradient reveals five fractions. After infection a sixth fraction appears. It contains virioins but mostly modified membranes with subunits 5-6 nm in diameter, probably the hemagglutinin. None of the enzymes used was associated with this fraction. No modifica...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-103

    authors: Payment P,Ajdukovic D,Pavilanis V

    更新日期:1975-05-01 00:00:00

  • Oxylipin and mitochondrion probes to track yeast sexual cells.

    abstract::When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests incre...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W08-035

    authors: Ncango DM,Swart CW,Goldblatt ME,Pohl CH,Van Wyk PW,Botes PJ,Kock JL

    更新日期:2008-06-01 00:00:00

  • Swarming motility by Photorhabdus temperata is influenced by environmental conditions and uses the same flagella as that used in swimming motility.

    abstract::Photorhabdus temperata, an insect pathogen and nematode symbiont, is motile in liquid medium by swimming. We found that P. temperata was capable of surface movement, termed swarming behavior. Several lines of evidence indicate that P. temperata use the same flagella for both swimming and swarming motility. Both motili...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W10-119

    authors: Michaels B,Tisa LS

    更新日期:2011-03-01 00:00:00

  • Zerumbone inhibits Candida albicans biofilm formation and hyphal growth.

    abstract::Candida albicans biofilm formation is considered an important matter because it can lead to strong resistance to conventional antifungal agents. Hyphae formed by C. albicans can also act as an important virulence factor related to its biofilm. The objective of this study was to determine the effect of zerumbone, a mon...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2019-0155

    authors: Shin DS,Eom YB

    更新日期:2019-10-01 00:00:00

  • Aerobic adaptation in yeast. I. Changes in metabolic intermediates during a step-down anaerobic-aerobic transfer.

    abstract::Early investigation into the mechanism of mitochondriogenesis in the facultative anaerobe Saccharomyces cerevisiae used anaerobic-aerobic transitions as model systems to study the in vivo assembly of these oxidative organelles. This methodology has become entrenched in the early literature and as a result a definitive...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m75-126

    authors: Bruver RM,Ball JS,Tustanoff ER

    更新日期:1975-06-01 00:00:00

  • Host cell invasion and intracellular residence by Aeromonas salmonicida: role of the S-layer.

    abstract::Virulent strains of the fish pathogen Aeromonas salmonicida, which have surface S-layers (S+), efficiently adhere to, enter, and survive within macrophages. Here we report that S+ bacteria were 10- to 20-fold more adherent to non-phagocytic fish cell lines than S-layer-negative (S-) mutants. When reconstituted with ex...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w00-034

    authors: Garduño RA,Moore AR,Olivier G,Lizama AL,Garduño E,Kay WW

    更新日期:2000-07-01 00:00:00

  • A turbidimetric and electron microscopic study of the effects of several parameters on the lysis of Streptococcus faecalis by lysozyme.

    abstract::The lytic effect of lysozyme on Streptococcus faecalis ATCC 9790 was studied by spectrophotometry and electron microscopy and it was found to be highly dependent on the ionic strength of the suspending media and on the ratio lysozyme to bacterial cell mass. When 7.2 X 10(8) bacteria/mL are exposed to 0.4 mg/mL of lyso...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m84-142

    authors: Carvalho ME,Gonçalves MH,Silva MT

    更新日期:1984-07-01 00:00:00

  • Efficient production of Aschersonia placenta protoplasts for transformation using optimization algorithms.

    abstract::The insect pathogenic fungus Aschersonia placenta is a highly effective pathogen of whiteflies and scale insects. However, few genetic tools are currently available for studying this organism. Here we report on the conditions for the production of transformable A. placenta protoplasts using an optimized protocol based...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2015-0770

    authors: Wei X,Song X,Dong D,Keyhani NO,Yao L,Zang X,Dong L,Gu Z,Fu D,Liu X,Qiu J,Guan X

    更新日期:2016-07-01 00:00:00

  • Inhibition of growth, iron, and sulfur oxidation in Thiobacillus ferrooxidans by simple organic compounds.

    abstract::Iron and sulfur oxidation by Thiobacillus ferrooxidans as well as growth on ferrous iron were inhibited by a variety of low molecular weight organic compounds. The influences of chemical structure of the organic inhibitors, pH, temperature, physical treatment of cells, and added inhibitory or stimulatory inorganic ion...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-105

    authors: Tuttle JH,Dugan PR

    更新日期:1976-05-01 00:00:00

  • A survey of indigenous microbial hydrocarbon degradation genes in soils from Antarctica and Brazil.

    abstract::Total community DNA from 29 noncontaminated soils and soils impacted by petroleum hydrocarbons and chloro-organics from Antarctica and Brazil were screened for the presence of nine catabolic genes, encoding alkane monooxygenase or aromatic dioxygenases, from known bacterial biodegradation pathways. Specific primers an...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-008

    authors: Luz AP,Pellizari VH,Whyte LG,Greer CW

    更新日期:2004-05-01 00:00:00

  • Biodegradation of the nitramine explosives hexahydro-1,3,5-trinitro-1,3,5-triazine and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine in cold marine sediment under anaerobic and oligotrophic conditions.

    abstract::The in situ degradation of the two nitramine explosives, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), was evaluated using a mixture of RDX and HMX, incubated anaerobically at 10 degrees C with marine sediment from a previous military dumping site of unexplod...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w03-112

    authors: Zhao JS,Greer CW,Thiboutot S,Ampleman G,Hawari J

    更新日期:2004-02-01 00:00:00

  • Enterotoxin formation by Clostridium perfringens type A studied by the use of fluorescent antibody.

    abstract::Fluorescein isothiocyanate-conjugated antibody to purified enterotoxin of Clostridium perfringens was used to study the intracellular formation of enterotoxin by this organism. Enterotoxin was detected at 4 h of growth at the end of the cell containing forespore. With the development of the spore, enterotoxin accumula...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m77-134

    authors: Niilo L

    更新日期:1977-07-01 00:00:00

  • Internalization of Mycobacterium shottsii and Mycobacterium pseudoshottsii by Acanthamoeba polyphaga.

    abstract::Amoebae serve as environmental hosts to a variety of mycobacteria, including Mycobacterium avium and Mycobacterium marinum. Mycobacterium shottsii and Mycobacterium pseudoshottsii are waterborne species isolated from the spleens and dermal lesions of striped bass (Morone saxatilis) from the Chesapeake Bay. The optimal...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2013-0079

    authors: Gupta T,Fine-Coulson K,Karls R,Gauthier D,Quinn F

    更新日期:2013-08-01 00:00:00

  • Induction of C4-dicarboxylate transport genes by external stimuli in Rhizobium meliloti.

    abstract::We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in a dctA gene. Levels of dctA gene expression ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m92-008

    authors: Batista S,Castro S,Aguilar OM,Martínez-Drets G

    更新日期:1992-01-01 00:00:00

  • Direct lymphocytotoxicity against herpes simplex virus infected cells.

    abstract::This study was undertaken to determine if direct cytotoxicity (DC) against herpes simplex virus infected cells, perhaps mediated by T cells, could be demonstrated in individuals subject to recurrent herpes labialis. The mononuclear cells from 7 out of 17 individuals with recurrent herpes expressed DC whereas no DC was...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m78-177

    authors: Fujimiya Y,Babiuk LA,Rouse BT

    更新日期:1978-09-01 00:00:00

  • Stress-survival responses of a carbon-starved p-nitrophenol-mineralizing Moraxella strain in river water.

    abstract::The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1-2 d. In the buffer system, the 1- and...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w04-131

    authors: Moore M,Trevors J,Lee H,Leung KT

    更新日期:2005-03-01 00:00:00

  • The nucleotide sequence of herpes simplex virus type 2 (333) glycoprotein gB2 and analysis of predicted antigenic sites.

    abstract::The gene coding for glycoprotein B2 (gB2) of herpes simplex virus type 2 (HSV-2) strain 333 was mapped and its nucleotide sequence determined. Open reading frame analysis deduced a polypeptide consisting of 902 amino acids and having close homology to gB1 of HSV type 1. Several predicted features of gB2 are consistent...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m87-154

    authors: Zwaagstra JC,Leung WC

    更新日期:1987-10-01 00:00:00

  • Hydrogen cyanide production by Pseudomonas aeruginosa at reduced oxygen levels.

    abstract::Hydrogen cyanide production by Pseudomonas aeruginosa growing in a synthetic medium required aerobosis but operated efficiently at low dissolved oxygen concentration. Half maximum levels of cyanogenesis occurred at 0.015 microM oxygen; maximum cyanogenesis occurred over a wide range, 0.1-180 microM, of oxygen concentr...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m83-209

    authors: Castric PA

    更新日期:1983-10-01 00:00:00

  • The in vitro hydroxylation of 4'-chloro-4-biphenylol by a mushroom tyrosinase preparation.

    abstract::Incubation of 4'-chloro-4-biphenylol with a mushroom tyrosinase preparation gave the catechol, 4'-chloro-3,4-biphenyldiol as the sole in vitro metabolite. This compound was identical with the major rat urinary metabolite of 4'-chloro-4-biphenylol and thus confirms the structure assigned to the metabolite. This result ...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m76-014

    authors: Safe S,Ellis BE,Hutzinger O

    更新日期:1976-01-01 00:00:00

  • Purification and chemical characterization of an exopolysaccharide isolated from Capnocytophaga ochracea.

    abstract::Purification and chemical characterization of an immunosuppressive exopolysaccharide from Capnocytophaga ochracea strain 25 are described. This polysaccharide was extracted from spent culture medium by cold ethanol precipitation. Purification was accomplished by trichloroacetic acid and pronase treatments in combinati...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m85-001

    authors: Dyer JK,Bolton RW

    更新日期:1985-01-01 00:00:00

  • Staphylococcus aureus strains differ in their in vitro responsiveness to human urokinase: evidence that methicillin-resistant strains are predominately nonresponsive to the growth-enhancing effects of urokinase.

    abstract::Clinical isolates of Staphylococcus aureus were found to exhibit strain-specific heterogeneity to the growth-enhancing effects of human urokinase (UK), a proteinase with plasminogen activator activity. Nine out of fourteen (64%) methicillin-sensitive strains of S. aureus were responsive to UK in "in vitro" cultures. I...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/m96-131

    authors: Hart DA,Louie T,Krulicki W,Reno C

    更新日期:1996-10-01 00:00:00

  • Microbial surfactant activities from a petrochemical landfarm in a humid tropical region of Brazil.

    abstract::The goal of this study was to assess the presence and surfactant potential of naturally occurring microbes from a tropical soil with petrochemical contamination. Microorganisms in a soil sample from a Brazilian landfarm were isolated and grown on petroleum as the sole carbon source. Of 60 isolates screened for petrole...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/W07-052

    authors: Maciel BM,Dias JC,Dos Santos AC,Filho RC,Fontana R,Loguercio LL,Rezende RP

    更新日期:2007-08-01 00:00:00

  • Isolation and diversity of natural product biosynthetic genes of cultivable bacteria associated with marine sponge Mycale sp. from the coast of Fujian, China.

    abstract::The marine sponge Mycale sp., a potential source of natural bioactive products, is widely distributed along the coast of Fujian, China. The cultivable bacterial community associated with Mycale sp., the antibacterial activities, and the PKS (polyketide synthase) and NRPS (nonribosomal peptide synthetase) gene diversit...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/cjm-2013-0785

    authors: Su P,Wang DX,Ding SX,Zhao J

    更新日期:2014-04-01 00:00:00

  • Selection and characterization of Spiroplasma citri mutants by random transposome mutagenesis.

    abstract::Phytopathogenic spiroplasmas can multiply in vascular plants and insects. A deeper understanding of this dual-host life could be furthered through the identification by random mutagenesis of spiroplasma genes required. The ability of the EZ::TN™ Tnp transposome™ system to create random insertional mutations i...

    journal_title:Canadian journal of microbiology

    pub_type: 杂志文章

    doi:10.1139/w11-026

    authors: Mutaqin K,Comer JL,Wayadande AC,Melcher U,Fletcher J

    更新日期:2011-06-01 00:00:00