Abstract:
:Bifidobacteria are generally acknowledged as major gut microflora used as probiotics, which promote human health. In this study, the effects of the administration of Bifidobacterim bifidum on modulating gastrointestinal (GI) tract microflora, acid and bile resistance, and physiological indices in BALB/c mice were investigated. Results showed that B. bifidum can significantly improve the ecosystem of the GI tract by increasing the amount of probiotics and reducing the populations of pathogenic bacteria, as measured by plate count and real-time PCR. After exposure to simulated GI tract conditions, the growth of gut microflora in the B. bifidum group was higher than that in the control group when incubated for 12 h in MRS or nutrient broth adjusted to pH 2.0 or 3.0 or in the presence of a concentration of bile salt (0.45% m/v). The blood biochemical index was examined, and the physiological effect of the cell-free extract of gut microflora was evaluated by measuring the activity of various enzymes, including α-glucosidases, esterase, and lactate dehydrogenase. This study suggested that a B. bifidum strain can stabilize blood sugar, lower cholesterol levels in serum, and improve metabolic activity. Moreover, B. bifidum was a promising enhancer of microbial diversity in mouse intestine and played a vital role in human physiological processes, which can benefit the health of a host.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Wang BG,Xu HB,Wei H,Zeng ZL,Xu Fdoi
10.1139/cjm-2014-0694subject
Has Abstractpub_date
2015-02-01 00:00:00pages
155-63issue
2eissn
0008-4166issn
1480-3275journal_volume
61pub_type
杂志文章abstract::Ninety-five fecal samples from Atlantic cod (Gadus morhua L.), caught along the northern Norwegian coast, were examined bacteriologically for occurrence of C. perfringens. Isolates were examined by polymerase chain reaction (PCR) for genes encoding the four lethal toxins (alpha, beta, epsilon, and iota) for classifica...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-015
更新日期:2002-04-01 00:00:00
abstract::This research validates a novel approach for source tracking based on denaturing gradient gel electrophoresis (DGGE) analysis of DNA extracted from Escherichia coli isolates. Escherichia coli from different animal sources and from river samples upstream from, at, and downstream of a combined sewer overflow were subjec...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-083
更新日期:2007-10-01 00:00:00
abstract::The induction of nitrogenase (C2H2) activity in asymbiotically cultured Rhizobium sp. 32H1 was found to be associated with morphological changes in the cells which were more pronounced than those seen in bacteroids. Polyphosphate granules were found in both bacteroids and cultured cells, but poly-beta-hydroxybutyrate ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-055
更新日期:1979-03-01 00:00:00
abstract::The possibility of using the volatile-producing fungus Muscodor albus for biofumigation against building molds was investigated. Several species of Aspergillus and Penicillium as well as fungi belonging to nine other genera were inhibited or killed in vitro by volatiles produced by potato dextrose agar or rye grain cu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W06-124
更新日期:2007-03-01 00:00:00
abstract::Samples of seawater, sediment, and the following invertebrates were collected from the Mediterranean Sea in the neighborhood of Alexandria, Egypt: sea urchins (Echinus spp.), clams (Tapes spp.), and wedge shells (Donax trunculus). A total of 165 samples were collected from November 1979 to July 1980. Average counts (p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-187
更新日期:1982-11-01 00:00:00
abstract::Biological nitrogen fixation (BNF) can be improved by optimizing the interaction between the rhizobial inoculant and pea (Pisum sativum L.), leading to increased productivity and reduced nitrogen (N) fertilizer use. Eight Rhizobium leguminosarum bv. viciae strains were used to inoculate the super-nodulating pea mutant...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2017-0281
更新日期:2017-11-01 00:00:00
abstract::Neisseria gonorrhoeae exist in transparent (Tr) and opaque (Op) colony forms. Op forms are recovered from patients early in the menstrual cycle; Tr colonies predominate late in the cycle. The mechanism for this colonial variation was examined by determining the influence of gonodal hormones on growth inhibition of Op ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-044
更新日期:1982-03-01 00:00:00
abstract::Ten strains of Caldariomyces fumago and related fungi were found to produce extracellular chloroperoxidase when grown on a glucose - malt extract medium. High enzyme levels and pigment production were observed for C. fumago ATCC 16373 and C. fumago CMI 89362. Removal of malt extract from the medium and the replacement...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m81-199
更新日期:1981-12-01 00:00:00
abstract::Nodulation of soybeans by indigenous and inoculum strains of Bradyrhizobium japonicum was studied in field experiments in Wisconsin from 1983 to 86. Aqueous suspensions of bacteria were applied to seeds at the time of planting at levels of 7 × 10(7)-10(10) bacteria per 2.5-cm row. The predominant indigenous serogroup ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m90-136
更新日期:1990-11-01 00:00:00
abstract::To assess the effect of fire and salvage logging on the diversity of mycorrhizal-bacterial communities, bacteria associated with Cenococcum, Thelephora, Tomentella, Russulaceae, and E-strain ectomycorrhizae (ECM) of Abies lasiocarpa seedlings were characterized using two approaches. First, bacteria were isolated and c...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w02-056
更新日期:2002-07-01 00:00:00
abstract::Clinical isolates of Staphylococcus aureus were found to exhibit strain-specific heterogeneity to the growth-enhancing effects of human urokinase (UK), a proteinase with plasminogen activator activity. Nine out of fourteen (64%) methicillin-sensitive strains of S. aureus were responsive to UK in "in vitro" cultures. I...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-131
更新日期:1996-10-01 00:00:00
abstract::Corynebacterium glutamicum, a gram-positive bacterium, has been widely used for industrial amino acid production. Corynebacterium glutamicum his genes are located and transcribed in two unlinked loci, hisEG and hisDCB-orf1-orf2-hisHA-impA-hisFI. The latter his operon starts the transcription at the C residue localized...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-115
更新日期:2010-02-01 00:00:00
abstract::Various soil fungi were tested for their capacity to degrade the insecticide [14C]photodieldrin. Of nine species investigated, Trichoderma viride was the only one which degraded the insecticide to an appreciable extent into water-soluble, non-insecticidal compounds within 4-5 weeks. These products amounted to 32-41% o...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-198
更新日期:1976-09-01 00:00:00
abstract::Thirty Haemophilus strains and six Actinobacillus strains, all of porcine origin, were examined for their biochemical reactivity on API 20E and API ZYM test strips using dense cell suspensions (supplemented with NAD as appropriate) as strip inocula. When combined with a test for V-factor dependency, the use of both st...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-195
更新日期:1984-10-01 00:00:00
abstract::A comparative study of the pathogenicity of Candida albicans morphological mutant strains was made to relate chlamydospore production, germ tube formation, and proteolytic activity to candidiasis in mice. It was observed that the mycelium strains were more lethal than the yeast-like strains and that neither chlamydosp...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-093
更新日期:1975-05-01 00:00:00
abstract::Fifty-two killer, factor-resistant, nuclear mutants were isolated from sensitive strains of yeast and assorted into three functional groups. All but one mutant owed their resistance to an alteration in the cell wall binding site for killer. In several mutant strains, an alteration at the site of killer binding was ass...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-041
更新日期:1978-03-01 00:00:00
abstract::Dark spots disease (DSD) is a relatively new coral disease that has become one of the most prevalent afflictions in the Caribbean Sea. To partially characterize bacterial communities associated with DSD, carbon utilization patterns of bacterial strains isolated from the surface mucopolysaccharide layers of healthy and...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-002
更新日期:2007-04-01 00:00:00
abstract::The new United States Drinking Water Regulations state that water systems must analyze for Escherichia coli or fecal coliforms on any routine or repeat sample that is positive for total coliforms. The proposed methods for the detection of E. coli are based on beta-glucuronidase activity, using the fluorogenic substrat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-157
更新日期:1991-12-01 00:00:00
abstract::Antibiotic-resistant and antimetabolite-resistant mutants of the nitrogen-fixing symbiotic bacterium Frankia were isolated to provide strains with genetic backgrounds amenable to genetic analysis. The lethal and mutagenic effects of ethyl methanesulfonate (EMS) and UV light on four Frankia strains were investigated. U...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-013
更新日期:2004-04-01 00:00:00
abstract::Mixtures of anionic HgCl3-/HgCl4(2)-complexes were less toxic to terrestrial bacteria (Erwinia herbicola, Agrobacterium tumefaciens), to marine bacteria (Acinetobacter sp., Aeromonas sp.), and to bacteriophages (phi 11 M 15 of Staphylococcus aureus and P1 of Escherichia coli) than were equivalent concentrations of Hg ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-197
更新日期:1979-11-01 00:00:00
abstract::Brachiaria, predominantly an African genus, contains species, such as B. brizantha, an apomictic C4 grass, that are commercially important forage grasses in tropical America, where they now cover about 55 million hectares. From B. brizantha accession CIAT 6780, we isolated an endophytic fungus that may be economically...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w00-127
更新日期:2001-01-01 00:00:00
abstract::Fluorescein isothiocyanate-conjugated antibody to purified enterotoxin of Clostridium perfringens was used to study the intracellular formation of enterotoxin by this organism. Enterotoxin was detected at 4 h of growth at the end of the cell containing forespore. With the development of the spore, enterotoxin accumula...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-134
更新日期:1977-07-01 00:00:00
abstract::Bacteriophages are associated with reduced fecal shedding of Shiga-toxin-producing Escherichia coli O157:H7 (STEC O157:H7) in cattle. Four phages exhibiting activity against 12 of 14 STEC O157:H7 strains, representing 11 common phage types, were isolated. Phages did not lyse non-O157 E. coli, with 11 of the 12 STEC st...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-063
更新日期:2012-07-01 00:00:00
abstract::Responses of Rhizoctonia solani to herbicides in soil cultures were assessed by measuring soil enzyme activity and other growth-related factors. Both beta-galactosidase (EC 3.2.1.23) and phosphatase (EC 3.1.3.1.3.1.3.2) activities were highly correlated with amounts of mycelium in soil. Both enzyme activities were red...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-089
更新日期:1977-05-01 00:00:00
abstract::Using both fluorescent labelled toxin and antibody--secondary antibody techniques, the Bacillus sphaericus toxin was found to bind strongly to susceptible Culex quinquefasciatus cells, but far less strongly to cells of insensitive insects. An insensitive clone of the C. quinquefasciatus cell line was discovered which ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m87-173
更新日期:1987-11-01 00:00:00
abstract::Mutants of Trichoderma harzianum with altered antibiotic production were isolated using ultraviolet light mutagenesis. These included strains whose activity in a Fusarium oxysporum spore germination assay was greater than twice that of the parental strain and one that had no detectable antifungal activity. Characteris...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-112
更新日期:1991-09-01 00:00:00
abstract::5-Methoxymethyl-2'-deoxyuridine (MMUdR), a drug with potent antiviral activity in vitro against Herpes simplex virus, was investigated for its immunosuppressive effects. Doses as high as 2000mg/kg given daily for 9 days were not immunosupporessive as judged by the fact that treated animals produced normal immune respo...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-158
更新日期:1977-08-01 00:00:00
abstract::Three distinct bacterial populations have been defined in the bovine rumen: the rumen fluid population; the population associated with food particles; and the population adherent to the rumen epithelium. Alkaline phosphatase activity has been reported in cells of the first two populations and here we report that assay...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-140
更新日期:1979-08-01 00:00:00
abstract::The effects of pressurized CO2 on the survival of Escherichia coli and the mechanism of cell inactivation were studied. Bacterial cultures were inoculated in nutrient broth and incubated at 30 degrees C for 18 h. Exposure of the cells to CO2 under pressures ranging from 2.5 to 25 MPa and at temperatures between 8 and ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-078
更新日期:2006-12-01 00:00:00
abstract::Ten bacteriophages infecting Micrococcus luteus have been characterized. All phages contain double-stranded DNA, of 64.3--73.5 mol% guanine plus cytosine (GC). The DNA of phage N7 has the highest GC content reported for any bacterial virus. No unusual bases have been found. The intracellular replicating DNAs of six ph...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-158
更新日期:1979-09-01 00:00:00