Mouse models of infection for Neisseria meningitidis B,2b and Haemophilus influenzae type b diseases.

Abstract:

:A disseminated and fatal infection was established in C57BL mice, injected intraperitoneally with either Neisseria meningitidis B,2b or Haemophilus influenzae type b bacteria plus enhancement factors. The effects of mucin, hemoglobin, and iron dextran as enhancement of bacterial infectivity in mice were evaluated individually and in combination. A mixture of mucin and hemoglobin was most effective in enhancing the virulence of the pathogens. Inbred mouse lines were more susceptible than outbred ones. Relative virulence of a number of bacterial strains was also compared in one selected mouse line. Neisseria meningitidis B,2b and Haemophilus influenzae type b strains were more virulent than non-B,2b and nontypable strains. Finally, the course of bacteremia for the two infections in mice was followed by quantitative blood cultures. The animals succumbed to the generalized condition within 72 h. In the case of Neisseria meningitidis B,2b, 10 organisms with 4% mucin and 1.6% hemoglobin were sufficient to kill 50% of the animals. For Haemophilus influenzae type b, 300 bacteria with 5% mucin and 2% hemoglobin were necessary to obtain similar effects.

journal_name

Can J Microbiol

authors

Brodeur BR,Tsang PS,Hamel J,Larose Y,Montplaisir S

doi

10.1139/m86-007

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

33-7

issue

1

eissn

0008-4166

issn

1480-3275

journal_volume

32

pub_type

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