Abstract:
:The catabolism of glucose by Streptomyces C5, a producer of anthracycline antibiotics, was investigated to determine the pathways that supply precursors for anthracycline biosynthesis. Carbons for the biosynthesis of epsilon-rhodomycinone, an anthracycline aglycone, from radiolabelled glucose were derived primarily from the Embden-Meyerhof-Parnas pathway, with a minor contribution from the pentose phosphate pathway. Furthermore, the anthracycline-producing strain, Streptomyces C5, as well as Streptomyces aureofaciens and Streptomyces lividans, strains that produce nonanthracycline polyketide antibiotics, displayed enzyme activities indicative of the Embden-Meyerhof-Parnas and pentose phosphate glycolytic pathways. As determined from labelling patterns, Streptomyces C5 apparently has a complete tricarboxylic acid cycle, but does not have a glyoxylate bypass pathway.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Dekleva ML,Strohl WRdoi
10.1139/m88-217subject
Has Abstractpub_date
1988-11-01 00:00:00pages
1235-40issue
11eissn
0008-4166issn
1480-3275journal_volume
34pub_type
杂志文章abstract::The PII protein is apparently involved in the control of NifA activity in Herbaspirillum seropedicae. To evaluate the probable role of PII in signal transduction, uridylylation assays were conducted with purified H. seropedicae PII and Escherichia coli GlnD, or a cell-free extract of H. seropedicae as sources of uridy...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w01-018
更新日期:2001-04-01 00:00:00
abstract::The 40-kDa porin protein of Haemophilus influenzae type b was reconstituted into proteoliposomes. The relative rates of diffusion of small uncharged sugars across the channels formed by this protein were determined by measuring the rates of osmotic swelling of the liposomes. From these rates, a pore diameter of 1.8 nm...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-027
更新日期:1988-02-01 00:00:00
abstract::Cells of five Bacteroides species were examined following treatment with homologous antisera and staining with ruthenium red. They were enveloped by glycocalyces and these extensive fibrous exopolysaccharide matrices were fully retained as an integral "capsule" by some cells, while other cells showed "capsule" as well...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m84-124
更新日期:1984-06-01 00:00:00
abstract::We report a cinematographic analysis of culmination in fruiting bodies of Polysphondylium pallidum. During the final stages of aggregation, sorogen elongation is slow. An increase in the rate of sorogen elongation occurs when the growing stalk contacts the substratum, lifting the cell mass into the air. The elongation...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-163
更新日期:1980-08-01 00:00:00
abstract::Fluorescein isothiocyanate-conjugated antibody to purified enterotoxin of Clostridium perfringens was used to study the intracellular formation of enterotoxin by this organism. Enterotoxin was detected at 4 h of growth at the end of the cell containing forespore. With the development of the spore, enterotoxin accumula...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m77-134
更新日期:1977-07-01 00:00:00
abstract::Bacterial communities that can degrade surfactants have been selected from coastal seawaters contaminated by urban sewages. Only the linear fraction of commercial anionic surfactants was quickly degraded, and a residual fraction representing 10% of the initial concentration always remained. The highest concentrations ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
abstract::Pyrenocine A, a phytotoxin produced by Pyrenochaeta terrestris (Hansen) Gorenz, Walker and Larson, possesses general antibiotic activity against plants, fungi, and bacteria. Effective doses for 50% inhibition (ED50s) are 4 micrograms/mL for onion seedling elongation; 14, 20, 20, and 25 micrograms/mL for the germinatio...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m87-055
更新日期:1987-04-01 00:00:00
abstract::Samples of seawater, sediment, and the following invertebrates were collected from the Mediterranean Sea in the neighborhood of Alexandria, Egypt: sea urchins (Echinus spp.), clams (Tapes spp.), and wedge shells (Donax trunculus). A total of 165 samples were collected from November 1979 to July 1980. Average counts (p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m82-187
更新日期:1982-11-01 00:00:00
abstract::Morphological differentiation has commanded attention for its putative impact on the pathogenesis of invasive fungal infections. We evaluated in vitro and in vivo the dimorphism from mycelial to yeast-phase of Sporothrix schenckii, Blastomyces dermatitidis and Paracoccidioides brasiliensis isolates, two strains for ea...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-026
更新日期:2004-06-01 00:00:00
abstract::Saccharomyces cerevisiae is a good model with which to study the effects of morphologic differentiation on the ecological behaviour of fungi. In this work, 33 morphologic mutants of a natural strain of S. cerevisiae, obtained with UV mutagenesis, were selected for their streak shape and cell shape on rich medium. Two ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W06-132
更新日期:2007-02-01 00:00:00
abstract::Fungal chitinases play essential roles in chitin degradation, cell wall remodeling, chitin recycling, nutrition acquisition, autolysis, and virulence. In this study, 18 genes of the glycoside hydrolase 18 (GH18) family were identified in the Isaria cicadae genome. Seventeen of the genes belonged to chitinases and one ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2019-0129
更新日期:2020-04-01 00:00:00
abstract::Eight murine monoclonal antibodies (MAbs) against staphylococcal enterotoxin D (SED) were obtained by fusion of myeloma cells with mouse spleen cells immunized with SED only or a combination of SED and either enterotoxin A (SEA) or enterotoxin E (SEE). When only SED was used as an immunogen, six MAbs were specific for...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m91-099
更新日期:1991-08-01 00:00:00
abstract::From the four known isoforms of the staphylococcal exfoliative toxins (ETs), only ETA and ETB are the major causative agents. General knowledge is that the gene for ETA is located on the chromosome, whereas that for ETB is located on a large plasmid. Yoshizawa and co-workers (2000, Microbiol. Immunol. 44(3): 189-191) ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0721
更新日期:2013-04-01 00:00:00
abstract::An anaerobic consortium that carboxylated and dehydroxylated phenol to benzoate, and 2-cresol to 3-methylbenzoic acid, under methanogenic conditions was studied. Phenol induced this transformation activity. Addition of 4-hydroxypyridine or an increase in the concentration of proteose peptone to 0.5% (w/v) delayed the ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-093
更新日期:1993-07-01 00:00:00
abstract::The metal-binding properties of Pseudomonas aeruginosa PAO1 biofilms were investigated using four metals (Cu, Fe, Au, and La). All but one of the metals (i.e., Cu) were bound by the biofilms in amounts that were significantly greater than those bound by planktonically grown cells of the same strain. Lanthanum precipit...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::The use of microbial fertilizers can help to avoid the harmful effects of traditional agricultural fertilizers and pesticides; however, there are many constraints on the practical application of such fertilizers. In this study, microbial biochar formulations (MBFs) were obtained by loading biochar, created from agricu...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2018-0333
更新日期:2019-05-01 00:00:00
abstract::Bacteria associated with 6 habitats of groundnut were evaluated for their broad-spectrum antifungal activity and suppression of collar rot (Aspergillus niger) of groundnut. Three hundred and ninety-three strains were tested against 8 fungal pathogens of groundnut including 5 necrotrophic fungi, Aspergillus flavus, A. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-119
更新日期:2005-02-01 00:00:00
abstract::A stable enrichment culture converting acetic acid to methane was successfully obtained from a pear waste digester, using a synthetic substrate solution with acetic acid as the main carbon source. This enrichment culture converted up to 10 mmol of acetic acid per litre per day at 35 degrees C and did not use hydrogen ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-194
更新日期:1976-09-01 00:00:00
abstract::The search for new antimicrobial compounds involves finding novel sources of chemotherapeutic compounds or manipulating and combining structures from existing molecules. Small antimicrobial peptides (AMPs) are components of innate immune defenses characterized in greatest detail in insect-derived AMPs. We have generat...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-001
更新日期:2009-05-01 00:00:00
abstract::Examination of immune complexes between cells of Streptococcus mutans and homologous antiserum by the techniques of thin-sectioning and freeze-etching revealed that the cells were embedded within and extensive matrix 80-90 nm thick with defined boundaries. ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-129
更新日期:1976-06-01 00:00:00
abstract::Mycoplasma pneumoniae is an important causative agent of atypical pneumonia. This study was to determine the ability of a DNA expression vector, which encodes the carboxy terminal region of the M. pneumoniae P1 protein (P1C), to induce humoral and cellular immune responses and to protect against M. pneumoniae infecti...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-041
更新日期:2012-05-01 00:00:00
abstract::Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-038
更新日期:2004-08-01 00:00:00
abstract::Cultures of Sclerotium rolfsii and Trichoderma viride together in autoclaved soil were assayed at intervals during 8 days of incubation for proteolytic activity (PA) of T. viride. Significant proteolytic activity was detected only in soil containing T. viride (i.e., T. viride alone or S. rolfsii + T. viride); greatest...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-079
更新日期:1978-04-01 00:00:00
abstract::The survival of and interactions between a Pseudomonas putida strain labelled with a red fluorescent protein gene (WT-rfp) and its green fluorescent protein gene-labelled rpoS(-) mutant (KO-gfp) were examined. The generation times of the planktonic WT-rfp and KO-gfp in trypticase soy broth were not significantly diffe...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w09-081
更新日期:2009-10-01 00:00:00
abstract::In mycetomes of leafhoppers, Helochara communis, ultrastructural and histochemical studies revealed that spheroplast symbiotes (t-symbiotes) were converted to 'a-symbiotes' (so-called), with apparent loss of DNA--a phenomenon reminiscent of rhizobium-bacteroid conversion in certain legume nodules. Additional t-symbiot...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-028
更新日期:1975-02-01 00:00:00
abstract::We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in a dctA gene. Levels of dctA gene expression ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m92-008
更新日期:1992-01-01 00:00:00
abstract::A new type of tube (the Lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. The Lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. Anaerobiosis is achieved through deoxygenation of the deep cylind...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-151
更新日期:1979-09-01 00:00:00
abstract::Products obtained from the action of arylamine synthetase on [G-14C]chorismic acid were fractionated by gel filtration and ion exchange column chromatography to yield a partially purified radioactive component with an arylamine function. From its ultraviolet absorption spectrum and thin-layer chromatographic behaviour...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-032
更新日期:1976-02-01 00:00:00
abstract::The cultural, morphological, and physiological properties of Thermomonospora fusca (strain 190Th) are described. Its physiological properties show that this species is primarily a carbohydrate-degrading actinomycete which can use a wide range of plant sugars and polymeric carbohydrates as sources of carbon and energy....
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-267
更新日期:1975-11-01 00:00:00
abstract::Canadian oil sands tailings are predominately sodic residues contaminated by hydrocarbons such as naphthenic acids. These conditions are harsh for plant development. In this study, we evaluated the effect of inoculating roots of Alnus viridis ssp. crispa and Alnus incana ssp. rugosa with ectomycorrhizal fungi in the p...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0703
更新日期:2016-07-01 00:00:00