Solid-phase polymerase chain reaction: applications for direct detection of enteric pathogens in waters.

abstract：:A rapid and efficient electroporation procedure has been developed for transformation of germinating conidia of filamentous fungi. Pretreatment of conidial preparations with a cell wall weakening agent, such as beta-glucuronidase, was found to be essential for successful transformation. Using the qa-2+ gene of Neurosp...

journal_title：Canadian journal of microbiology

authors： Chakraborty BN,Patterson NA,Kapoor M

更新日期：1991-11-01 00:00:00

abstract：:One of the proposed mechanisms by which rhizobacteria enhance plant growth is through the production of plant growth regulators. Five plant growth promoting rhizobacterial (PGPR) strains produced the cytokinin dihydrozeatin riboside (DHZR) in pure culture. Cytokinin production by Pseudomonas fluorescens G20-18, a rifa...

journal_title：Canadian journal of microbiology

authors： García de Salamone IE,Hynes RK,Nelson LM

更新日期：2001-05-01 00:00:00

abstract：:The catabolism of glucose by Streptomyces C5, a producer of anthracycline antibiotics, was investigated to determine the pathways that supply precursors for anthracycline biosynthesis. Carbons for the biosynthesis of epsilon-rhodomycinone, an anthracycline aglycone, from radiolabelled glucose were derived primarily fr...

journal_title：Canadian journal of microbiology

authors： Dekleva ML,Strohl WR

更新日期：1988-11-01 00:00:00

abstract：:This research examines the distribution of aflatoxins among conidia and sclerotia of toxigenic strains of Aspergillus flavus Link and Aspergillus parasiticus Speare cultured on Czapek agar (21 days, 28 degrees C). Total aflatoxin levels in conidia and sclerotia varied considerably both within (intrafungal) and among s...

journal_title：Canadian journal of microbiology

authors： Wicklow DT,Shotwell OL

更新日期：1983-01-01 00:00:00

abstract：:The anaerobic degradative pathway for metabolism of 2,4,6-trinitrotoluene (TNT) by a consortium of Desulfovibrio spp. isolated from a creek sediment was studied. This consortium has the metabolic capability to degrade TNT to fatty acids. The growth of the consortium and the metabolism of TNT were greatly enhanced in t...

journal_title：Canadian journal of microbiology

authors： Boopathy R,Manning JF

更新日期：1996-12-01 00:00:00

abstract：:Citrobacter freundii from an environmental source displayed resistance to a wide variety of antibacterials. Some of these resistances could be transferred by conjugation with frequencies as high as 10(-2). The complexity of plasmid involvement in the antibacterial resistance of this species was demonstrated by isolati...

journal_title：Canadian journal of microbiology

authors： Austen RA,Trust TJ

更新日期：1981-03-01 00:00:00

abstract：:Cultures of Sclerotium rolfsii and Trichoderma viride together in autoclaved soil were assayed at intervals during 8 days of incubation for proteolytic activity (PA) of T. viride. Significant proteolytic activity was detected only in soil containing T. viride (i.e., T. viride alone or S. rolfsii + T. viride); greatest...

journal_title：Canadian journal of microbiology

authors： Rodriguez-Kabana R,Kelley WD,Curl EA

更新日期：1978-04-01 00:00:00

abstract：:Nodulation of soybeans by indigenous and inoculum strains of Bradyrhizobium japonicum was studied in field experiments in Wisconsin from 1983 to 86. Aqueous suspensions of bacteria were applied to seeds at the time of planting at levels of 7 × 10(7)-10(10) bacteria per 2.5-cm row. The predominant indigenous serogroup ...

journal_title：Canadian journal of microbiology

authors： McLoughlin TJ,Alt SG,Merlo PA

更新日期：1990-11-01 00:00:00

abstract：:An anaerobic consortium that carboxylated and dehydroxylated phenol to benzoate, and 2-cresol to 3-methylbenzoic acid, under methanogenic conditions was studied. Phenol induced this transformation activity. Addition of 4-hydroxypyridine or an increase in the concentration of proteose peptone to 0.5% (w/v) delayed the ...

journal_title：Canadian journal of microbiology

authors： Bisaillon JG,Lépine F,Beaudet R,Sylvestre M

更新日期：1993-07-01 00:00:00

abstract：:Using a mixture of three mono nitrophenols as sole carbon, nitrogen and energy sources, mixed cultures were enriched from municipal activated sludge to degrade both nitrophenols and nitrobenzene. Bacterial growth and degradation rate could be increased by supplementing the medium with 0.1% YE. Microorganisms were isol...

journal_title：Canadian journal of microbiology

authors： Zhao JS,Ward OP

更新日期：1999-05-01 00:00:00

abstract：:The purpose of this study was to evaluate experimentally the immunogenicity in rabbits of rubella subunits adsorbed to the adjuvant Quil A. The adsorbed viral proteins form structurally defined ImmunoStimulating COMplexes (ISCOMs). Rubella ISCOMs were tested for their capacity to induce neutralizing and hemagglutinati...

journal_title：Canadian journal of microbiology

authors： Trudel M,Nadon F,Séguin C,Payment P

更新日期：1988-12-01 00:00:00

abstract：:Vesicular-arbuscular mycorrhizae of yellow poplar (Liriodendron tulipifera L.) were examined by scanning electron microscopy. The morphology and spatial distribution of endophytic structures are illustrated. The use of stereoscopic techniques in conjunction with removal of host cytoplasm permits detection of subtle ...

journal_title：Canadian journal of microbiology

authors： Kinden DA,Brown MF

更新日期：1975-07-01 00:00:00

abstract：:Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs ...

journal_title：Canadian journal of microbiology

authors： Lachance MA,Bowles JM,Starmer WT,Barker JS

更新日期：1999-02-01 00:00:00

abstract：:Grapes inoculated with poliovirus 1 and coxsackievirus B5 were washed with water, 0.5% polyehtylene glycol, or phosphate-buffered saline with 1% serum. These washes were equally efficient at removing virus but much of the virus in the water was noninfectious until treated with 0.5% polyethylene glycol. ...

journal_title：Canadian journal of microbiology

authors： Konowalchuk J,Spiers JI

更新日期：1977-09-01 00:00:00

abstract：:Natural populations of bacteria assoiciated with the slime on submerged surfaces in a mountain stream were examined by phase-contrast and electron microscopy. The slime contained large numbers of bacteria which were predominantly gram-negative as determined by their cell wall structure. Examination of the in situ dist...

journal_title：Canadian journal of microbiology

authors： Geesey GG,Richardson WT,Yeomans HG,Irvin RT,Costerton JW

更新日期：1977-12-01 00:00:00

abstract：:The regulation of de novo pyrimidine biosynthesis in Pseudomonas putida ATCC 17536 by pyrimidines was explored. The pathway enzyme activities were higher in glucose-grown cells than in succinate-grown cells, indicating catabolite repression by succinate. In P. putida cells grown on succinate as a carbon source, only a...

journal_title：Canadian journal of microbiology

authors： Santiago MF,West TP

更新日期：2002-12-01 00:00:00

abstract：:The course of intranasal infection of Sendai virus in CBA and DBA mice was investigated in animals decomplemented with purified cobra venom factor. The mice were decomplemented either immediately before inoculation or at 4 days postinfection. Depletion of complement after the infection had been established had no appa...

journal_title：Canadian journal of microbiology

authors： Finnie JC,Aston WP,O'Shaughnessy MV,Stewart RB

更新日期：1982-05-01 00:00:00

abstract：:Antibiotic susceptibilities of 382 strains of Campylobacter jejuni isolated in Alberta, Canada, in 1980 and 1981 were determined. Although none were resistant to erythromycin or gentamicin, 5.4 and 22% of strains were resistant to ampicillin in 1980 and 1981, respectively. Tetracycline resistance was noted in 6.8% of ...

journal_title：Canadian journal of microbiology

authors： Taylor DE,Chang N,Garner RS,Sherburne R,Mueller L

更新日期：1986-01-01 00:00:00

abstract：:Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20 degrees C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent micr...

journal_title：Canadian journal of microbiology

authors： Kogure K,Simidu U,Taga N

更新日期：1979-03-01 00:00:00

abstract：:Campylobacter fetus is a recognized pathogen of cattle and sheep that can also infect humans. No adhesins specific for C. fetus have to date been identified; however, bacterial attachment is essential to establish an infecting population. Scanning electron microscopy revealed C. fetus attachment to the serosal surface...

journal_title：Canadian journal of microbiology

authors： Graham LL,Friel T,Woodman RL

更新日期：2008-01-01 00:00:00

abstract：:Characterization and enumeration of the adherent epimural community of the rumen wall of young, conventionally reared lambs were carried out from 2 to 21 days after birth. Three hundred strains were isolated by anaerobic procedures from three sites: dorsal, ventral, and caudal sacs, and from the sloughed epithelial ce...

journal_title：Canadian journal of microbiology

authors： Rieu F,Fonty G,Gouet P

更新日期：1989-07-01 00:00:00

abstract：:To facilitate the recognition of plant viruses with similar characteristics a dendrogram of characterized viruses was constructed. The sequence of criteria included: type of nucleic acid; single or double stranded; presence or absence of lipid envelope; helical or nonhelical symmetry; and divided or single genome. Non...

journal_title：Canadian journal of microbiology

authors： Tremaine JH

更新日期：1977-09-01 00:00:00

abstract：:The insect pathogenic fungus Aschersonia placenta is a highly effective pathogen of whiteflies and scale insects. However, few genetic tools are currently available for studying this organism. Here we report on the conditions for the production of transformable A. placenta protoplasts using an optimized protocol based...

journal_title：Canadian journal of microbiology

authors： Wei X,Song X,Dong D,Keyhani NO,Yao L,Zang X,Dong L,Gu Z,Fu D,Liu X,Qiu J,Guan X

更新日期：2016-07-01 00:00:00

abstract：:Two strains of Bacillus sphaericus. SSII-1 and 1593, were bioassayed for toxic activity against second-instar larvae of the mosquito Culex pipiens quinquefasciatus. It was found that strain 1593 developed a level of toxicity 3000 times that of strain SSII-1. Although the toxic activity of B. sphaericus SSII-1 was rela...

journal_title：Canadian journal of microbiology

authors： Myers P,Yousten AA,Davidson EW

更新日期：1979-11-01 00:00:00

abstract：:This research validates a novel approach for source tracking based on denaturing gradient gel electrophoresis (DGGE) analysis of DNA extracted from Escherichia coli isolates. Escherichia coli from different animal sources and from river samples upstream from, at, and downstream of a combined sewer overflow were subjec...

journal_title：Canadian journal of microbiology

authors： D'Elia TV,Cooper CR,Johnston CG

更新日期：2007-10-01 00:00:00

abstract：:Fusaproliferin (FP), a toxic metabolite of the world-wide maize pathogens Fusarium proliferatum and Fusarium subglutinans, was recently found to be a natural contaminant of maize. Its toxic activity on haematopoietic human cell lines and its teratogenic effects on chicken embryos has been recently proved. Therefore a ...

journal_title：Canadian journal of microbiology

authors： Monti SM,Fogliano V,Randazzo G,Peluso G,Logrieco A,Ritieni A

更新日期：1999-01-01 00:00:00

abstract：:The IncHII plasmid pHH1508a (208 kilobases) encodes resistance to potassium tellurite, trimethoprim, and streptomycin. Conjugative pili encoded by pHH1508a were isolated, purified, and used for preparation of anti-H pilus antiserum. Immuno-gold labelling experiments using H pilus specific antiserum showed that antigen...

journal_title：Canadian journal of microbiology

authors： Yan W,Taylor DE

更新日期：1989-02-01 00:00:00

abstract：:The range of polysaccharide-degrading enzymes formed by three anaerobic rumen fungi (Neocallimastix patriciarum, Piromonas communis, and an unidentified isolate (F] was monitored following growth on seven mono-, di-, and poly-saccharide carbohydrate substrates. Enzymes capable of degrading a variety of alpha- and beta...

journal_title：Canadian journal of microbiology

authors： Williams AG,Orpin CG

更新日期：1987-05-01 00:00:00

abstract：:Phialocephala fortinii is a dark septate fungal endophyte that colonizes roots of many host species. Its effect on plant growth varies from being pathogenic to beneficial. The basic biology of this species has received little research, and thus the main objectives of this study were to determine cytological features o...

journal_title：Canadian journal of microbiology

authors： Saito K,Kuga-Uetake Y,Saito M,Peterson RL

更新日期：2006-07-01 00:00:00

abstract：:An alginate lyase gene of a newly isolated Pseudomonas sp. strain KS-408 was cloned by using PCR with the specific primers designed from homologous nucleotide sequences. A partial protein sequence of KS-408 alginate lyase was homology-modeled on the basis of the crystal structure of A1-III alginate lyase from Sphingom...

journal_title：Canadian journal of microbiology

authors： Kam N,Park YJ,Lee EY,Kim HS

更新日期：2011-12-01 00:00:00