Abstract：
:The range of polysaccharide-degrading enzymes formed by three anaerobic rumen fungi (Neocallimastix patriciarum, Piromonas communis, and an unidentified isolate (F] was monitored following growth on seven mono-, di-, and poly-saccharide carbohydrate substrates. Enzymes capable of degrading a variety of alpha- and beta-glucans, beta-galactans, galactomannan, and hemicellulosic arabinoxylans were present in all three isolates. Although reducing saccharides were released from pectin, polygalacturonic acid was not degraded by the preparations. Enzyme activity was present in both the zoospore and vegetative stages of the life cycle and was also detected extracellularly in culture supernatants after vegetative growth. The specific activities of the polysaccharidases were affected by the growth substrate, being lowest in preparations grown on mono- and di-saccharides, whereas polysaccharidic growth substrates resulted in increased activity of the corresponding polysaccharidases. The enzymes were, however, formed after growth on all substrates. Oligomers and monosaccharides were produced as a result of polysaccharide breakdown by the unfractionated enzyme preparations. Studies on hemicellulose (arabinoxylan) breakdown by unfractionated vegetative preparations of the three isolates indicated that their modes of action, pH optima, substrate affinities, and response to potential inhibitors were similar.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Williams AG,Orpin CGdoi
10.1139/m87-071subject
Has Abstractpub_date
1987-05-01 00:00:00pages
418-26issue
5eissn
0008-4166issn
1480-3275journal_volume
33pub_type
杂志文章abstract：:New antibiotics produced by Streptomyces echinatus A8331 cultured in the presence of heterocyclic aromatic acids can be separated and purified by high-performance liquid chromatography using reversed phase columns. Natural quinoxaline antibiotics and certain quinoline derivatives can also be efficiently separated in n...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-112
更新日期：1984-06-01 00:00:00
abstract：:Vibrio cholerae activates proinflammatory response in cultured intestinal epithelial cells. In this study, we demonstrate that V. cholerae O395 infection of intestinal epithelial cells results in the activation of Akt. Inhibition of Akt significantly decreases IL-1alpha, IL-6, and TNF-alpha production in V. cholerae i...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-093
更新日期：2009-11-01 00:00:00
abstract：:The lytic effect of lysozyme on Streptococcus faecalis ATCC 9790 was studied by spectrophotometry and electron microscopy and it was found to be highly dependent on the ionic strength of the suspending media and on the ratio lysozyme to bacterial cell mass. When 7.2 X 10(8) bacteria/mL are exposed to 0.4 mg/mL of lyso...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-142
更新日期：1984-07-01 00:00:00
abstract：:In Salmonella typhimurium LT2 the F factor of Escherichia coli K-12 replicates normally but is repressed; Flac+ cells give no visible lysis on solid media with male-specific phages, low frequency transfer of Flac+ (0.001-0.007 per donor cell), few f2 infective centers (0.002-0.006 per cell), and they propagate male-sp...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-184
更新日期：1983-09-01 00:00:00
abstract：:This study aimed to examine aminoglycosides (AMGs) resistance mechanisms, including the AMG-modifying enzyme genes, mexXY, rplY, nuoG, and galU, in the Pseudomonas aeruginosa non-cystic fibrosis (CF) isolates in Thailand. One hundred P. aeruginosa isolates from non-CF patients were examined for susceptibility to AMGs ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2012-0465
更新日期：2013-01-01 00:00:00
abstract：:An intracellular invertase was induced in cultures of Clostridium pasteurianum utilizing sucrose as its carbon source for growth. This enzyme synthesis could be repressed by the addition of fructose of a sucrose-growing culture. In contrast, invertase activity was not affected by the addition of glucose to sucrose-gro...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-251
更新日期：1975-11-01 00:00:00
abstract：:The aim of the present study was to explore a new approach based on the hemagglutination (HA) assay to understand the colonization ability of Shigella spp. To study colonization ability, an animal model of 4-day-old suckling mouse, was exploited. We characterized the HA activity of 48 Shigella strains, with erythroc...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w2012-095
更新日期：2012-10-01 00:00:00
abstract：:A method to obtain synchronous sporulation during idiophase development in liquid surface cultures of Rhizopus arrhizus Fischer in described and evaluated for use in studying metabolism associated with asexual sporulation. In this system growth and sporulation are effectively separated. The respiratory pattern indicat...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-151
更新日期：1977-08-01 00:00:00
abstract：:The altitudinal distribution of microfungi associated with Betula ermanii Cham. leaf litter was investigated on Mt. Rishiri (1719 m), northern Japan. Fallen leaves were collected at five sites of different altitudes (approximately 300, 600, 900, 1200, and 1500 m above sea level) along a transect on the northern slope ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w09-030
更新日期：2009-07-01 00:00:00
abstract：:In this study, the biofilm-forming potential of intestinal Lactobacillus reuteri strains under different culture conditions was characterized by microtiter plate biofilm assays. Moreover, the spatial organization of exogenously applied L. reuteri L2/6 (a pig isolate) at specific locations in gastrointestinal tract of ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0007
更新日期：2015-06-01 00:00:00
abstract：:Salmonella typhimurium and a series of rough lipopolysaccharide mutants derived from it were used as target bacteria to examine the antimicrobial capacity of magainin 2. Magainin 2 demonstrated a dose-related bactericidal activity against the smooth parent strain and the series of lipopolysaccharide mutants. The lipop...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-102
更新日期：1990-08-01 00:00:00
abstract：:A morphological and physiological description of an anamorphic basidiomycetous yeast species, named Trichosporon guehoae (CBS 8521T), is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nu...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w99-055
更新日期：1999-08-01 00:00:00
abstract：:Monoclonal antibodies reacting with the B subunit of Vibrio cholerae O1 strain 569B cholera toxin (CT-B) were used to identify unique and common epitopes of V. cholerae non-O1 and Vibrio mimicus CT-B. Vibrio cholerae non-O1 strains produced CT-B showing three monoclonal antibody reaction patterns (epitypes), which cor...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-071
更新日期：1990-06-01 00:00:00
abstract：:The types of bacteria and fungi present in the air of a municipal solid waste recovery system have been characterized and the population densities estimated. Conventional methods were successful in enumerating coagulase-positive staphylococci, Klebsiella spp., gram-negative bacteria, fungi, and common indicator organi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m85-038
更新日期：1985-03-01 00:00:00
abstract：:The binding of mutagenic pyrolyzates to cell fractions from some gram-negative intestinal bacteria and to thermally treated bacterial cells was investigated. 3-Amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) were effectively bound by several of the bacterial ce...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-101
更新日期：1992-07-01 00:00:00
abstract：:Sixty-four species of filamentous fungi from five flare pits in northern and western Canada were tested for their ability to degrade crude oil using gas chromatographic analysis of residual hydrocarbons following incubation. Nine isolates were tested further using radiorespirometry to determine the extent of mineraliz...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:The application of crossed immunoelectrophoresis to the analysis of a reference cytoplasm and cell envelope preparation from Pasteurella multocida serotype 1 revealed antigenic complexity not previously found. At least 55 cytoplasmic and 19 cell envelope antigens were clearly distinguished. Variation of anticytoplasm ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-118
更新日期：1980-06-01 00:00:00
abstract：:The peptidoglycan of Escherichia coli K-12 strain AB264 was isolated by treating whole cells with sodium dodecyl sulfate and was purified by deoxyribonuclease, ribonuclease, and trypsin treatment. Like the peptidoglycan of Bacillus subtilis, this peptidoglycan proved able to bind substantial amounts of metallic ions f...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m84-031
更新日期：1984-02-01 00:00:00
abstract：:The repair of DNA damage caused by ultraviolet radiation (UVR) is well understood in both lower and higher organisms. Genetic studies carried out at optimum temperature for growth, 37 °C in Escherichia coli, have revealed the major pathways of DNA repair. We show that E. coli cells grown at 20 °C are more sensitive to...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2013-0874
更新日期：2014-05-01 00:00:00
abstract：:Nodulation of soybeans by indigenous and inoculum strains of Bradyrhizobium japonicum was studied in field experiments in Wisconsin from 1983 to 86. Aqueous suspensions of bacteria were applied to seeds at the time of planting at levels of 7 × 10(7)-10(10) bacteria per 2.5-cm row. The predominant indigenous serogroup ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m90-136
更新日期：1990-11-01 00:00:00
abstract：:In Japan, antimicrobial agent therapy for patients with diarrhea due to enterovirulent organisms including enterohemorrhagic Escherichia coli (EHEC) is common, and norfloxacin (NFLX), fosfomycin, and kanamycin are recommended for EHEC treatment by the Japanese Ministry of Health and Welfare. The aim of this study was ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：:Mutants of Salmonella typhimurium with defects in the heptose region of the lipopolysaccharide (LPS) molecule (heptose-deficient, chemotype Re) leak periplasmic enzymes (acid phosphatase (EC 3.1.3.2), cyclic phosphodiesterase, ribonuclease I (EC 3.1.4.22), and phosphoglucose isomerase (EC 5.3.1.9) (PGI is at least par...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-227
更新日期：1976-10-01 00:00:00
abstract：:The metal-binding properties of Pseudomonas aeruginosa PAO1 biofilms were investigated using four metals (Cu, Fe, Au, and La). All but one of the metals (i.e., Cu) were bound by the biofilms in amounts that were significantly greater than those bound by planktonically grown cells of the same strain. Lanthanum precipit...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:A series of synthetic promoters, based upon the Escherichia coli sigma 70 consensus promoter sequence, was constructed upstream of the lacZ reporter gene in the modified broad-host-range vector pQF52. The role of the intervening spacer region in gene expression in Pseudomonas aeruginosa and E. coli was studied by inse...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-141
更新日期：1997-10-01 00:00:00
abstract：:Approximately 20% of Canada's oil supply now comes from the extraction of bitumen from the oil sands deposits in northeastern Alberta. The oil sands are strip-mined, and the bitumen is typically separated from sand and clays by an alkaline hot water extraction process. The rapidly expanding oil sands industry has mill...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-129
更新日期：2002-01-01 00:00:00
abstract：:An alginate lyase gene of a newly isolated Pseudomonas sp. strain KS-408 was cloned by using PCR with the specific primers designed from homologous nucleotide sequences. A partial protein sequence of KS-408 alginate lyase was homology-modeled on the basis of the crystal structure of A1-III alginate lyase from Sphingom...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-106
更新日期：2011-12-01 00:00:00
abstract：:Neisseria gonorrhoeae strains can be grouped or differentiated (auxotyped) by their requirements for none, or any one or more of proline, uracil, hypoxanthine, and citrulline or ornithine. Most strains were readily assessed because they responded with growth or no growth on each defined auxanographic medium. Other str...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-204
更新日期：1983-10-01 00:00:00
abstract：:Nitrogenase in Azotobacter chroococcum whole cells was inhibited by enzymically generated superoxide anion (O2-), hydrogen peroxide, and ethyl hydrogen peroxide. The degree of inhibition produced by O2- was related to the quantity of oxygen supplied to the organisms in continuous cultures. O2- also inhibited oxygen up...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-228
更新日期：1977-11-01 00:00:00
abstract：:Cloning and transcriptional regulation of the KlFBA1 gene that codes for the class II fructose-1,6-bisphosphate aldolase of the yeast Kluyveromyces lactis are described. KlFBA1 mRNA diminishes transiently during the shift from hypoxic to fully aerobic conditions and increases in the reversal shift. This regulation is ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-038
更新日期：2004-08-01 00:00:00
abstract：:Menadione reductase (EC 1.6.99.2) has been purified 67-fold from Clostridium tyrobutyricum extracts. The molecular weight was found to be 60 000 and the prosthetic group was identified as FMN on the basis of the enzymatic analysis data. The binding of FMN to the menadione dehydrogenase apoenzyme was relatively weak wi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-053
更新日期：1980-03-01 00:00:00
