Abstract:
:Transcriptional profiles of 2 unrelated clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates were analyzed following 10% (v/v) ethanol challenge (15 min), which arrested growth but did not reduce viability. Ethanol-induced stress (EIS) resulted in differential gene expression of 1091 genes, 600 common to both strains, of which 291 were upregulated. With the exception of the downregulation of genes involved with osmotic stress functions, EIS resulted in the upregulation of genes that contribute to stress response networks, notably those altered by oxidative stress, protein quality control in general, and heat shock in particular. In addition, genes involved with transcription, translation, and nucleotide biosynthesis were downregulated. relP, which encodes a small alarmone synthetase (RelP), was highly upregulated in both MRSA strains following ethanol challenge, and relP inactivation experiments indicated that this gene contributed to EIS growth arrest. A number of persistence-associated genes were also upregulated during EIS, including those that encode toxin-antitoxin systems. Overall, transcriptional profiling indicated that the MRSA investigated responded to EIS by entering a state of dormancy and by altering the expression of elements from cross protective stress response systems in an effort to protect preexisting proteins.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Pando JM,Pfeltz RF,Cuaron JA,Nagarajan V,Mishra MN,Torres NJ,Elasri MO,Wilkinson BJ,Gustafson JEdoi
10.1139/cjm-2017-0221subject
Has Abstractpub_date
2017-09-01 00:00:00pages
745-757issue
9eissn
0008-4166issn
1480-3275journal_volume
63pub_type
杂志文章abstract::Pseudomonas aeruginosa ATCC 9027 grew on 0.5% (v/v) hexadecane as a sole carbon source in a chemically defined medium which required the addition of Fe3+ and Ca2+. There was a variable and extended lag period before an active growth rate was attained. Visible light microscopic evidence revealed that the bacteria did n...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-049
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journal_title:Canadian journal of microbiology
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doi:10.1139/m77-089
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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doi:10.1139/m96-035
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-055
更新日期:1979-03-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::alpha-Methyl lysine was investigated as a potential inhibitor of lysine transport in Escherichia coli and Bacillus sphaericus. At equimolar concentrations, no inhibition was observed in either organism, but at 10X and 100X the lysine concentration, alpha-methyl lysine caused a 20-50% reduction in the initial rate of l...
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journal_title:Canadian journal of microbiology
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