Simplified procedures for releasing and concentrating microorganisms from soil for transmission electron microscopy viewing as thin-sectioned and frozen-etched preparations.

abstract：:Chemical formation of a glucose-cyanide complex was necessary for metabolic degradation of cyanide at concentrations up to 50.0 mg/L by a strain of Escherichia coli isolated from gold extraction circuit liquids. Ammonia accumulating during the culture log phase as the sole nitrogen by-product was further utilized for ...

journal_title：Canadian journal of microbiology

authors： Figueira MM,Ciminelli VS,de Andrade MC,Linardi VR

更新日期：1996-05-01 00:00:00

abstract：:Campylobacter fetus is a recognized pathogen of cattle and sheep that can also infect humans. No adhesins specific for C. fetus have to date been identified; however, bacterial attachment is essential to establish an infecting population. Scanning electron microscopy revealed C. fetus attachment to the serosal surface...

journal_title：Canadian journal of microbiology

authors： Graham LL,Friel T,Woodman RL

更新日期：2008-01-01 00:00:00

abstract：:Symbiotic nitrogen fixation may be limited by the transport of C4 dicarboxylates into bacteroids in the nodule for use as a carbon and energy source. In an attempt to increase dicarboxylate transport, a plasmid was constructed in which the Rhizobium meliloti structural transport gene dctA was fused to a tryptophan ope...

journal_title：Canadian journal of microbiology

authors： Rastogi V,Labes M,Finan T,Watson R

更新日期：1992-06-01 00:00:00

abstract：:The fatty acid pattern in Mycobacterium vaccae strain JOB5 was examined after growth on n-alkanes (C14-C18), 1-alkenes (C14-C18), 2- or 3-methyl octadecane, and 8-heptadecene. It was evident that monoterminal oxidation of n-alkanes was followed by beta-oxidation and that both parent fatty acid and products of beta-oxi...

journal_title：Canadian journal of microbiology

authors： King DH,Perry JJ

更新日期：1975-01-01 00:00:00

abstract：:The presence of killer and proteolytic yeasts was studied among 944 isolates representing 105 species from tropical yeast communities. We found 13 killer toxin producing species, with Pichia kluyveri being the most frequent. Other killer yeast isolates were Candida apis, Candida bombicola, Candida fructus, Candida kru...

journal_title：Canadian journal of microbiology

authors： Abranches J,Morais PB,Rosa CA,Mendonça-Hagler LC,Hagler AN

更新日期：1997-04-01 00:00:00

abstract：:Plant endophytes are a group of microorganisms that reside asymptomatically within the healthy living tissue. The diversity and molecular and biochemical characterization of industrial hemp-associated endophytes have not been previously studied. This study explored the abundance and diversity of culturable endophytes ...

journal_title：Canadian journal of microbiology

authors： Scott M,Rani M,Samsatly J,Charron JB,Jabaji S

更新日期：2018-10-01 00:00:00

abstract：:The biochemical reactions, carbohydrate content, and 16S-rRNA sequences of Tatlockia (Legionella) maceachernii and Tatlockia micdadei strains were studied. Except for catalase activity, Tatlockia strains were relatively inert in the biochemical tests commonly used in clinical laboratories. Phenotypically, the two Tatl...

journal_title：Canadian journal of microbiology

authors： Fox KF,Brown A

更新日期：1993-05-01 00:00:00

abstract：:alpha-Methyl lysine was investigated as a potential inhibitor of lysine transport in Escherichia coli and Bacillus sphaericus. At equimolar concentrations, no inhibition was observed in either organism, but at 10X and 100X the lysine concentration, alpha-methyl lysine caused a 20-50% reduction in the initial rate of l...

journal_title：Canadian journal of microbiology

authors： Regenstreif CA,Kelland JG,Pansare SV,Vederas JC,Pickard MA

更新日期：1986-06-01 00:00:00

abstract：:The 40-kDa porin protein of Haemophilus influenzae type b was reconstituted into proteoliposomes. The relative rates of diffusion of small uncharged sugars across the channels formed by this protein were determined by measuring the rates of osmotic swelling of the liposomes. From these rates, a pore diameter of 1.8 nm...

journal_title：Canadian journal of microbiology

authors： Vachon V,Kristjanson DN,Coulton JW

更新日期：1988-02-01 00:00:00

abstract：:The microbial community plays an important role in soil nutrient cycles and energy transformations in alpine grassland. In this study, we investigated the composition of the soil microbial community collected from alpine cold swamp meadow (ASM), alpine cold meadow (AM), and alpine cold desert steppe (ADS) within the B...

journal_title：Canadian journal of microbiology

authors： Shao K,Gao G

更新日期：2018-03-01 00:00:00

abstract：:Antagonistic activity of the bacterium Pseudomonas cepacia against Trichoderma viride was greatly influenced by nutritional and environmental conditions. Xylose and trehalose strongly enhanced the antifungal activity of P. cepacia, whereas mannitol and glucose had little effect. The carbon sources that enhanced the an...

journal_title：Canadian journal of microbiology

authors： Upadhyay RS,Visintin L,Jayaswal RK

更新日期：1991-11-01 00:00:00

abstract：:The ultraviolet germicidal irradiation (UVGI) dose necessary to inactivate fungal spores on an agar surface and the efficacy of UVGI were determined for cultures of Stachybotrys chartarum (ATCC 208877). This study employed a UVGI testing unit consisting of four chambers with a 9-W, Phillips, low pressure, mercury UVGI...

journal_title：Canadian journal of microbiology

authors： Green CF,Davidson CS,Scarpino PV,Gibbs SG

更新日期：2005-09-01 00:00:00

abstract：:Lyme disease is a tick-borne disease that is emerging in Canada. The disease is caused by spirochetes of the Lyme borreliosis group, which is expanding as new species are discovered. In Canada, Lyme disease risk has so far been assessed primarily by detection of Borrelia burgdorferi sensu stricto. Of Ixodes scapularis...

journal_title：Canadian journal of microbiology

authors： Lewis J,Lloyd VK

更新日期：2019-02-01 00:00:00

abstract：:Several restriction systems were detected in different strains of Streptomyces antibioticus by using actinophages as biological indicators. Adsorption of phages to the bacteria, together with the study of the efficiency of plating gave an initial indication of restriction in three strains. The alternation of efficienc...

journal_title：Canadian journal of microbiology

authors： Sànchez J,Barbès C,Hernandez A,de los Reyes Gavilàn CR,Hardisson C

更新日期：1985-10-01 00:00:00

abstract：:The induction of antituberculous immunity by crude ribosomal fractions isolated from Mycobacterium tuberculosis strain H37Ra, M. bovis strain BCG, and M. smegmatis was studied in CF-1 mice. Levels of antituberculous immunity similar to that induced by live BCG were induced by the BCG and H37Ra ribosomal fractions wher...

journal_title：Canadian journal of microbiology

authors： Portelance V,Beaudet R

更新日期：1983-02-01 00:00:00

abstract：:We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...

journal_title：Canadian journal of microbiology

authors： White AP,Allen-Vercoe E,Jones BW,DeVinney R,Kay WW,Surette MG

更新日期：2007-01-01 00:00:00

abstract：:A microplaque reduction assay for human and mouse interferons is described, using plastic disposable multicompartmental plates with flat-bottomed wells of 6 mm in diameter. The procedure is rapid and reproducible and lends itself to a considerable degree of automation. Its potential application in other areas of virol...

journal_title：Canadian journal of microbiology

authors： Campbell JB,Grunberger T,Kochman MA,White SL

更新日期：1975-08-01 00:00:00

abstract：:Menadione reductase (EC 1.6.99.2) has been purified 67-fold from Clostridium tyrobutyricum extracts. The molecular weight was found to be 60 000 and the prosthetic group was identified as FMN on the basis of the enzymatic analysis data. The binding of FMN to the menadione dehydrogenase apoenzyme was relatively weak wi...

journal_title：Canadian journal of microbiology

authors： Petitdemange H,Marczak R,Raval G,Gay R

更新日期：1980-03-01 00:00:00

abstract：:Environmental factors influencing the occurrence and community structure of soil yeasts in forests are not well studied. There are few studies dedicated to Southern Hemisphere soil yeasts populations and even fewer focused on temperate forests influenced by volcanic activity. The present work aimed to study the ecolog...

journal_title：Canadian journal of microbiology

authors： Mestre MC,Fontenla S,Rosa CA

更新日期：2014-06-01 00:00:00

abstract：:Four hundred and forty-two serogroup C Neisseria meningitidis isolates from individual invasive meningococcal disease (IMD) patients in Canada during the period 1999 to 2003 were analyzed. The majority (84%) of the serogroup C meningococci were characterized by the serotype antigen 2a and belonged to the clonal comple...

journal_title：Canadian journal of microbiology

authors： Law DK,Stoltz J,Henderson AM,Tsang RS

更新日期：2005-07-01 00:00:00

abstract：:Andosols comprise one of the most important soil groups for agricultural activities in Japan because they cover about 46.5% of arable upland fields. In this soil group, available phosphorus (P) is accumulated by application of excessive fertilizer, but little is known about the influence of increasing P availability o...

journal_title：Canadian journal of microbiology

authors： Bao Z,Matsushita Y,Morimoto S,Hoshino YT,Suzuki C,Nagaoka K,Takenaka M,Murakami H,Kuroyanagi Y,Urashima Y,Sekiguchi H,Kushida A,Toyota K,Saito M,Tsushima S

更新日期：2013-06-01 00:00:00

abstract：:Previously, we reported the metabolic responses of Pseudomonas sp. strain HF-1, a nicotine-degrading bacterium, to nicotine stress. However, the metabolic effects of nicotine on non-nicotine-degrading bacteria that dominate the environment are still unclear. Here, we have used nuclear magnetic resonance based metabolo...

journal_title：Canadian journal of microbiology

authors： Ding L,Chen J,Zou J,Zhang L,Ye Y

更新日期：2014-08-01 00:00:00

abstract：:The aim of the present investigation was to isolate a probiotic strain from 23 samples of yurts cheese and 21 samples of kumiss (collected from scattered households in Xinjiang and Inner Mongolia), and from eN-Lac Capsules, a health-promoting product. The isolates were subjected to biochemical characterization analysi...

journal_title：Canadian journal of microbiology

authors： Kou X,Chen Q,Ju X,Liu H,Chen W,Xue Z

更新日期：2014-11-01 00:00:00

abstract：:In mycetomes of leafhoppers, Helochara communis, ultrastructural and histochemical studies revealed that spheroplast symbiotes (t-symbiotes) were converted to 'a-symbiotes' (so-called), with apparent loss of DNA--a phenomenon reminiscent of rhizobium-bacteroid conversion in certain legume nodules. Additional t-symbiot...

journal_title：Canadian journal of microbiology

authors： Chang KP,Musgrave AJ

更新日期：1975-02-01 00:00:00

abstract：:The tuberculin activity of protoplasmic extracts isolated from living BCG was purified successively by gel filtration on Sephadex G-100 and G-75, and by electrophoresis on 7.5% and on gradient (6-18%) polyacrylamide gels. The tuberculin-active fractions, as determined in BCG-sensitized guinea pigs, were used as the st...

journal_title：Canadian journal of microbiology

authors： Laguerre M,Turcotte R

更新日期：1975-12-01 00:00:00

abstract：:The purpose of this study was to examine antimicrobial activity of endophytic fungi isolated from the leaves, stems, and roots of 54 species of Orchidaceae collected in a Brazilian tropical ecosystem. In total, 382 filamentous fungi and 13 yeast isolates were obtained and cultured to examine the production of crude ex...

journal_title：Canadian journal of microbiology

authors： Vaz AB,Mota RC,Bomfim MR,Vieira ML,Zani CL,Rosa CA,Rosa LH

更新日期：2009-12-01 00:00:00

abstract：:Five yeast strains belonging to the genus Cryptococcus Vuillemin were isolated from steppe plants and turf collected in the Prioksko-terrasny biosphere reserve (Moscow region, Russia). Sequence analyses of the D1/D2 domains of the 26S rDNA and of the internal transcribed spacer region revealed that these yeast strains...

journal_title：Canadian journal of microbiology

authors： Golubev WI,Sampaio JP,Alves L,Golubev NW

更新日期：2004-12-01 00:00:00

abstract：:Alkaline phosphatase (APase) was present in the bovine rumen in both cell-free and cell-associated states and levels of the enzyme varied with dietary regime. Reaction product deposition showed that the enzyme was associated with the mixed bacterial population. No enzyme was observed to be associated with protozoa. Tr...

journal_title：Canadian journal of microbiology

authors： Cheng KJ,Hironaka R,Costerton JW

更新日期：1976-05-01 00:00:00

abstract：:A species of Arthrobacter was recovered during culture of the causal organism of pitch canker of southern pines. Fusarium moniliforme var. subglutinans (FMS). Arthrobacter is a relatively common soil bacterium and is lytic to several fungal pathogens in the soil. Soil samples from two seed orchards with pitch canker a...

journal_title：Canadian journal of microbiology

authors： Barrows-Broaddus J,Kerr TJ

更新日期：1981-01-01 00:00:00

abstract：:The N5,N10-methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum strain Marburg has been purified with reasonable yield and much higher specific activity than previously reported. For the first time it has been shown that both N5,N10-methylenetetrahydromethanopterin dehydrogenase and...

journal_title：Canadian journal of microbiology

authors： Mukhopadhyay B,Daniels L

更新日期：1989-04-01 00:00:00