Abstract：
:Long-term survival, dispersal, and germination of Bacillus thuringiensis var. kurstaki DMU67R has been investigated in a field trial. An experimental cabbage plot was sprayed with DMU67R in 1993 and allowed to lie fallow since. The investigations reported here were carried out from 1997 to 2000 in this plot. High persistence of DMU67R for 7 years in the bulk soil of the plot has been demonstrated. The numbers have not significantly reduced since 1994, stabilizing around 6.6 x 10(2) cfu/g from 1996 to 2000. Horizontal dispersal of DMU67R in the 1994-1999 period was limited. Vertical dispersal occurred from 1994 to 1999, as 77% of the population of DMU67R occurred in the 0-2 cm layer in 1994, while only 22% of the population was found there in 1999. Most of the population in 1999 was present homogeneously in the upper 6 cm of the soil profile. Germination, as evidenced by the ratio of DMU67R cfu before and after heat treatment, was not observed in the bulk soil. However, in the rhizospheres of dandelion (Taraxacum officinalis) and quackgrass (Agropyron repens), 40 and 50% of DMU67R was present as vegetative germinated cells, respectively. No germination occurred in the rhizosphere of red fescue (Festuca rubra). The material from the gut of the earthworm species Lumbricus rubellus, Lumbricus terrestris, and Apporrectodea caliginosa and from a tipulid larvae from the plot also contained vegetative cells of DMU67R. Further investigations of A. caliginosa showed that germination seemed to be restricted to the gut and that sporulation occurred after defecation. The germination of DMU67R in rhizospheres and in the gut of nontarget invertebrates suggests that survival in the soil of B. thuringiensis is a dynamic process involving germination, cell divisions, and sporulation in specific microhabitats.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Hendriksen NB,Hansen BMdoi
10.1139/w02-009subject
Has Abstractpub_date
2002-03-01 00:00:00pages
256-61issue
3eissn
0008-4166issn
1480-3275journal_volume
48pub_type
杂志文章abstract：:A high molecular component of a saline extract derived from Listeria monocytogenes contained amino acids, carbohydrates, and phosphorus. The same fraction was capable of promoting both the in vitro mitogenic and adjuvant activities and the in vivo immunosuppressive activity displayed by the crude extract. The material...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-204
更新日期：1982-12-01 00:00:00
abstract：:Vesicular-arbuscular mycorrhizae of yellow poplar (Liriodendron tulipifera L.) were examined by scanning electron microscopy. The morphology and spatial distribution of endophytic structures are illustrated. The use of stereoscopic techniques in conjunction with removal of host cytoplasm permits detection of subtle ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-146
更新日期：1975-07-01 00:00:00
abstract：:Epiphytic tracer bacteris (a Pseudomonas sp. and an Arthrobacter sp.) from soybean buds were introduced into open flowers of greenhouse-grown soybean plants. Of the 177 resulting pods cultured after surface disinfection, tracers were recovered from within 24. Seed from two of these pods also carried tracers. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-064
更新日期：1976-03-01 00:00:00
abstract：:Menadione reductase (EC 1.6.99.2) has been purified 67-fold from Clostridium tyrobutyricum extracts. The molecular weight was found to be 60 000 and the prosthetic group was identified as FMN on the basis of the enzymatic analysis data. The binding of FMN to the menadione dehydrogenase apoenzyme was relatively weak wi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-053
更新日期：1980-03-01 00:00:00
abstract：:Hydrogen cyanide production by Pseudomonas aeruginosa growing in a synthetic medium required aerobosis but operated efficiently at low dissolved oxygen concentration. Half maximum levels of cyanogenesis occurred at 0.015 microM oxygen; maximum cyanogenesis occurred over a wide range, 0.1-180 microM, of oxygen concentr...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-209
更新日期：1983-10-01 00:00:00
abstract：:We have previously reported that the lethal toxicity of 5-fluorouracil (5-FU) in specific-pathogen-free mice is due to an indigenous infection with Escherichia coli (K. Nomoto, T. Yokokura, Y. Yoshikai, et al. Can. J. Microbiol. 37:244-247, 1991). In the present study, we demonstrate that nonspecific immunostimulation...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-126
更新日期：1992-08-01 00:00:00
abstract：:This study, the first of arbovirus activity in Prince Edward Island, has shown antibodies to the snowshoe hare strain of the California encephalitis group virus to be present in sera of wild and domestic animals; 15.35% of snowshoe hare, 20% of equine. and 2.5% of bovine sera were positive for hemagglutination inhibit...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-188
更新日期：1981-11-01 00:00:00
abstract：:Investigations of microbial diversity in Antarctic are important to begin to understand ecosystem functioning and decomposition processes. This study documents fungi at 9 historic sites on the Antarctic Peninsula collected from wooden structures, other organic materials, and soils during a joint National Science Found...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W08-120
更新日期：2009-01-01 00:00:00
abstract：:Several probes for measuring dissolved carbon dioxide (CO2) concentration were installed in a 68-litre fermentor and their effectiveness compared. Submerged silastic rubber tubing gave reproducible results over a wide range of operating conditions and was generally superior to all other probes evaluated. The silastic ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-007
更新日期：1976-01-01 00:00:00
abstract：:The poly(3-hydroxybutyrate) (PHB) depolymerase structural gene of Comamonas sp. (phaZCsp) was cloned in Escherichia coli and identified by halo formation on PHB-containing solid medium. The nucleotide sequence of a 1719 base pair MboI fragment was determined and contained one large open reading frame (ORF1, 1542 base ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m95-183
更新日期：1995-01-01 00:00:00
abstract：:The susceptibility of congenitally immunodeficient mice to a nonencapsulated strain of Cryptococcus neoformans (strain M7) was evaluated. Gnotobiotic mice with defined congenital defects in innate immunity (beige) or cell-mediated immunity (athymic) or with combined defects in innate and cellular immunity (beige athym...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-144
更新日期：1991-11-01 00:00:00
abstract：:Methanospirillum hungatei strains GP1 and JF1 when exposed to the adenosinetriphosphatase inhibitor N,N'-dicyclohexylcarbodiimide experienced a marked decline in growth rates, methane synthesis activities, and intracellular ATP concentrations. Although growth was inhibited, the intracellular ATP concentrations of all ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m82-146
更新日期：1982-08-01 00:00:00
abstract：:A species of Arthrobacter was recovered during culture of the causal organism of pitch canker of southern pines. Fusarium moniliforme var. subglutinans (FMS). Arthrobacter is a relatively common soil bacterium and is lytic to several fungal pathogens in the soil. Soil samples from two seed orchards with pitch canker a...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-004
更新日期：1981-01-01 00:00:00
abstract：:Lymphocytic choriomeningitis virus could be demonstrated earlier in mice inoculated with clinical specimens if the mice were sacrificed before they appeared sick for examination by the fluorescent antibody technique. In the procedure, the optimal route for inoculation was intracerebral and the best organ tested for st...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-245
更新日期：1976-11-01 00:00:00
abstract：:A bacteriocin, streptococcin sal-P, was isolated by freeze-thaw elution from cultures of Streptococcus salivarius strain P grown on tryptic soy agar plus 1% neopeptone. The inhibitor could also be extracted with either 7 M urea of 1 M NaCl from cells grown on this medium, but little activity was recovered from cells g...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m81-144
更新日期：1981-09-01 00:00:00
abstract：:This study compared the recovery of Cryptosporidium oocysts and Giardia cysts ((oo)cysts) from raw waters using 4 different concentration-elution methods: flatbed membranes, FiltaMax foam, Envirochek HV capsules, and Hemoflow ultrafilters. The recovery efficiency of the combined immunomagnetic separation and staining ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-059
更新日期：2004-09-01 00:00:00
abstract：:Polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (DNA-DNA) duplexes with endonuclease S1. In addition, DNA base composition (mole % guanine and cytosine (G + C)) and relative genome sizes were determined for selected strains...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-083
更新日期：1979-05-01 00:00:00
abstract：:Several strains of Rhizobium japonicum have been reported to consist of mixtures of stable derivativess having distinct colony morphologies and physiological characteristics. We isolated derivatives from strains of R. japonicum and systematically compared them with previously isolated derivatives with respect to the u...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-106
更新日期：1980-05-01 00:00:00
abstract：:An alginate lyase gene of a newly isolated Pseudomonas sp. strain KS-408 was cloned by using PCR with the specific primers designed from homologous nucleotide sequences. A partial protein sequence of KS-408 alginate lyase was homology-modeled on the basis of the crystal structure of A1-III alginate lyase from Sphingom...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w11-106
更新日期：2011-12-01 00:00:00
abstract：:The oomycetes, a distinct phylogenetic lineage of fungus-like microorganisms, are heterokonts (stramenopiles) belonging to the supergroup Chromalveolata. Although the complete genomic sequences of a number of oomycetes have been reported, little information regarding the introns therein is available. Here, we investig...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w10-103
更新日期：2011-02-01 00:00:00
abstract：:Intestinal infections are a major cause of mortality in domestic rabbits. Although a few pathogenic organisms have been associated with this type of infection, it is often impossible to demonstrate the presence of any etiologic agents. Recently viral particles were observed in the intestine of diseased rabbits submitt...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-201
更新日期：1980-10-01 00:00:00
abstract：:The effect of a chromated-copper-arsenate wood preservative on the degradation of pentachlorophenol by Flavobacterium sp. strain ATCC 53874 was examined in liquid culture. Both a commercially available and a laboratory-prepared formulation were tested. Each increased the lag time required for measurable pentachlorophe...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m94-063
更新日期：1994-05-01 00:00:00
abstract：:The antiserum agar method (ASA), which is based on the formation of immunoprecipitates around bacterial growth on agar containing meningococcal hyperimmune horse serum, was evaluated for serogroup identification of Neisseria meningitidis. Four hundred meningococcal stains were serogrouped by ASA employing horse antise...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-113
更新日期：1979-06-01 00:00:00
abstract：:A commercially available single-bottle blood culture system was evaluated at Ben Taub General Hospital, a Harris County District Hospital. Blood cultures from 1010 patients were examined with the Lederle Diagnostics one-bottle blood culture medium-SPS, Columbia broth (E-Vac, Pfizer), and an in-house-prepared brain hea...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-155
更新日期：1977-08-01 00:00:00
abstract：:Mice immunized subcutaneously with 10(6) viable yeast cells of Histoplasma capsulatum exhibited host protection to a challenge infection and also exhibited concomitant manifestations of cellular immunity to a culture filtrate antigen (yeast extract dialysate (YED)-histoplasmin). Protective immunity was determined by q...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-016
更新日期：1983-01-01 00:00:00
abstract：:Aspergillus fumigatus conidia have been linked to severe aspergillosis in immunocompromised patients. Recently, the cytotoxic effect of secondary metabolites from A. fumigatus conidia was reported. In the present work, a methodology used to detect cell death markers in fungal hyphae was adapted to study conidia cell d...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2015-0561
更新日期：2016-02-01 00:00:00
abstract：:Bacterial community structure and the effects of several environmental factors on the microbial community distribution were investigated in the sediment of the eutrophic Lake Xuanwu. Profiles of bacterial communities were generated using denaturing gradient gel electrophoresis (DGGE), and the results were interpreted ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W08-098
更新日期：2008-12-01 00:00:00
abstract：:Frankia are gram-positive, filamentous bacteria capable of fixing atmospheric dinitrogen in symbiosis with a wide variety of woody plants and shrubs. Some isolates of Frankia harbor plasmids of 8.5 (pFQ11) and 22.4 kb (pFQ12) that have no known function but are transmitted through many generations in culture. We have ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-050
更新日期：2001-07-01 00:00:00
abstract：:Concentric bodies were found in vegetative and pycnidial cells of Podoxythium tricothecium and in ascostromal paraphyses of Rhytidhysterium rufulum, two representatives of the Loculoascomycetes. They averages 185 nm in diameter in P. trichothecium and 255 nm in R. rufulum and consisted of three zones. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m77-218
更新日期：1977-10-01 00:00:00
abstract：:During growth on minimal salts - glucose media supplemented with high concentrations of Na2SO3 (10-3 and 10-2 M), Salmonella heidelberg exhibited cytological and growth responses which indicated increased cellular toxicity with increasing sulfite concentrations. The large quantities of sulfide evolved during growth at...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-217
更新日期：1979-12-01 00:00:00
