Abstract:
:Aspergillus fumigatus conidia have been linked to severe aspergillosis in immunocompromised patients. Recently, the cytotoxic effect of secondary metabolites from A. fumigatus conidia was reported. In the present work, a methodology used to detect cell death markers in fungal hyphae was adapted to study conidia cell death. Additionally, the mechanism of H2O2-induced cell death was studied in A. fumigatus conidia for the first time. Data presented in this work reveal that the H2O2-induced conidial cell death was associated with a marked increase of TUNEL- and PI-positive cells. It is therefore suggested that conidia cell death occurs in a dose-dependent manner through a secondary necrosis mechanism. The knowledge of conidia cell death machinery may provide insights into the molecular mechanism of conidia-mediated toxicity to the respiratory tract and may pave the way for improved therapeutic approaches against A. fumigatus conidia-mediated diseases.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Oliveira M,Pereira C,Bessa C,Araujo R,Saraiva Ldoi
10.1139/cjm-2015-0561subject
Has Abstractpub_date
2016-02-01 00:00:00pages
95-101issue
2eissn
0008-4166issn
1480-3275journal_volume
62pub_type
杂志文章abstract::BaeR, a response regulator protein, takes part in multidrug efflux, bacterial virulence activity, and other biological functions. Recently, BaeR was shown to induce inflammatory responses by activating the mitogen-activated protein kinases (MAPKs). In this study, we investigated additional pathways used by BaeR to ind...
journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
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pub_type: 杂志文章
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pub_type: 杂志文章
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