Abstract：
:Fermentation properties under the control of multiple genes are difficult to alter with traditional methods in Saccharomyces cerevisiae. Here, a novel genome engineering approach is developed to improve ethanol production in very high gravity fermentation with 300 g/L glucose as the carbon source. This strategy involved constructing aneuploid strains on the base of tetraploid cells. The tetraploid strain was constructed by using the plasmid YCplac33-GHK, which harbored the HO gene encoding the site-specific Ho endonucleases. The aneuploid strain, WT4-M, was selected and screened after the tetraploid cells were treated with methyl benzimidazole-2-yl-carbamate to induce loss of mitotic chromosomes. It was found that aneuploid strain WT4-M not only exhibited an increase in ethanol production and osmotic and thermal tolerance, but also an improvement in the sugar-ethanol conversion rate. Notably, WT4-M provided up to 9.8% improvement in ethanol production compared with the control strain. The results demonstrated that the strategy of aneuploidy was valuable for creating yeast strains with better fermentation characteristics.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Hou L,Cao X,Wang Cdoi
10.1139/w10-032subject
Has Abstractpub_date
2010-06-01 00:00:00pages
495-500issue
6eissn
0008-4166issn
1480-3275pii
w10-032journal_volume
56pub_type
杂志文章abstract：:We have characterized two mutants of Rhizobium meliloti L5-30 obtained by random mutagenesis using Mu-lacZ that were defective in transport of C4-dicarboxylic acids. These mutants induced ineffective nodules on alfalfa. Mutations in the two strains appeared to be located in a dctA gene. Levels of dctA gene expression ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m92-008
更新日期：1992-01-01 00:00:00
abstract：:A mesophilic anaerobe, a member of the Bacteroidaceae family (NRC2248), isolated from a cellulose-enrichment culture, digested untreated Whatman cellulose powder and HCl-treated cotton battings while producing hydrogen, carbon dioxide, cellobiose, glucose, and acetic acid as the sole volatile acid. This organism also ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m79-222
更新日期：1979-12-01 00:00:00
abstract：:Seven tk- mutants of herpes simplex virus, type 2 (HSV-2), and three tk- mutants of herpes simplex virus, type 1 (HSV-1), were isolated which did not produce the thymidine kinase (TK) polypeptides but formed smaller polypeptides not seen in wild-type infected cells. Positive TK mRNA selection by hybridization to the c...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m83-063
更新日期：1983-04-01 00:00:00
abstract：:Shiga toxins produced by Escherichia coli O157:H7 cause a wide spectrum of enteric diseases, such as lethal hemorrhagic colitis and hemolytic uremic syndrome. In this study, the B subunit protein of Shiga toxin type 1 (Stx1) was produced in the E. coli system, was further purified by Ni-column Affinity Chromatography ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/W10-087
更新日期：2010-12-01 00:00:00
abstract：:The effect of carbon starvation on the stress-resistant responses of a p-nitrophenol-mineralizing Moraxella strain was examined in both buffer and river water samples. The Moraxella strain showed optimal stress-resistant responses in a minimal salt buffer when carbon-starved for 1-2 d. In the buffer system, the 1- and...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-131
更新日期：2005-03-01 00:00:00
abstract：:The effects of various hydrocarbon substrates, and a chemical surfactant capable of enhancing crude-oil biodegradation, on the community structure of a mixed-bacterial inoculum were examined in batch culture. Of 1000 TSA-culturable isolates, 68.6% were identified at the genus level or better by phospholipid fatty acid...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:Bottles of medium were inoculated with both strains of Chromatiacea and Chlorobiaceae (mixed cultures) and immersed at differnt depths in the marine coastal waters of the Marseilles basin (France). After development, the in vivo absorption characteristics of the cells show the effect of the daylight penetration on the...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:A bacterium C1010, isolated from the rhizospheres of cucumbers in fields in Korea, degraded the microbial quorum-sensing molecules, hexanoyl homoserine lactone (HHSL), and octadecanoyl homoserine lactone (OHSL). Morphological characteristics and 16S rRNA sequence analysis identified C1010 as Acinetobacter sp. strain C...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w04-083
更新日期：2004-11-01 00:00:00
abstract：:Like recF and recQ mutants studied earlier, two other classes of Escherichia coli mutants defective in the RecF conjugal recombination pathway, recJ and recO, were found to be partially resistant to thymineless death. In contrast, a recN mutant, also belonging to the pathway, was indistinguishable from the wild type w...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-157
更新日期：1988-07-01 00:00:00
abstract：:Giardia duodenalis is a common intestinal parasite in most parts of the world. In Canada it is associated with both endemic and epidemic infections that are often transmitted by the waterborne route. Although G. duodenalis strains have been isolated from several animals, the role of other mammals in human infection is...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-171
更新日期：1986-12-01 00:00:00
abstract：:Examination of immune complexes between cells of Streptococcus mutans and homologous antiserum by the techniques of thin-sectioning and freeze-etching revealed that the cells were embedded within and extensive matrix 80-90 nm thick with defined boundaries. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-129
更新日期：1976-06-01 00:00:00
abstract：:Candida albicans biofilm formation is considered an important matter because it can lead to strong resistance to conventional antifungal agents. Hyphae formed by C. albicans can also act as an important virulence factor related to its biofilm. The objective of this study was to determine the effect of zerumbone, a mon...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2019-0155
更新日期：2019-10-01 00:00:00
abstract：:The biochemical reactions, carbohydrate content, and 16S-rRNA sequences of Tatlockia (Legionella) maceachernii and Tatlockia micdadei strains were studied. Except for catalase activity, Tatlockia strains were relatively inert in the biochemical tests commonly used in clinical laboratories. Phenotypically, the two Tatl...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-069
更新日期：1993-05-01 00:00:00
abstract：:A purified lipopolysaccharide (LPS) isolated from the rickettsial agent of Q fever, Coxiella burnetii phase 1, possesses some of the in vivo and in vitro biological properties previously only associated with bacterial endotoxins. The Coxiella LPS is mitogenic for guinea pig leukocytes, induces non-specific resistance ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m78-259
更新日期：1978-12-01 00:00:00
abstract：:Recent food-borne outbreaks of human listeriosis as well as numerous sporadic cases have been mainly caused by Listeria monocytogenes serovar 4b strains. Thus, it was of interest to find out whether a certain clone or a certain few clones were responsible for these cases and especially for outbreaks. We used pulsed-fi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m93-058
更新日期：1993-04-01 00:00:00
abstract：:The open reading frame pp0053, which has a high homology with the sequence of mitochondrial sulfide dehydrogenase (HMT2) conferring cadmium tolerance in fission yeast, was amplified from Pseudomonas putida KT2440 and expressed in Escherichia coli JM109(DE3). The isolated and purified PP0053-His showed absorption spect...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-022
更新日期：2006-08-01 00:00:00
abstract：:Approximately 20% of Canada's oil supply now comes from the extraction of bitumen from the oil sands deposits in northeastern Alberta. The oil sands are strip-mined, and the bitumen is typically separated from sand and clays by an alkaline hot water extraction process. The rapidly expanding oil sands industry has mill...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w01-129
更新日期：2002-01-01 00:00:00
abstract：:Pseudomonas aeruginosa ATCC 9027 grew on 0.5% (v/v) hexadecane as a sole carbon source in a chemically defined medium which required the addition of Fe3+ and Ca2+. There was a variable and extended lag period before an active growth rate was attained. Visible light microscopic evidence revealed that the bacteria did n...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m86-049
更新日期：1986-03-01 00:00:00
abstract：:Quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. The signal molecules involved in quorum sensing in P. carotovorum subsp. carotovorum belong to the group of N-acyl homoserine lactones (AHLs). In our study, we scre...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-062
更新日期：2006-10-01 00:00:00
abstract：:The mouse model of intraperitoneal sepsis with Proteus rettgeri was used to evaluate the anti-endotoxic effect of polymyxin B sulfate. An unexpected reversal of the usual protective effect of polymyxin in experimental enterobacterial sepsis was observed in which the lethality of the infection was enhanced. ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m80-039
更新日期：1980-02-01 00:00:00
abstract：:Soil bacteria that utilize inositol hexaphosphate (IHP) were isolated from a range of soils using defined selection media. An analysis of 200 randomly selected isolates indicated that less than 0.5% of the culturable population of soil bacteria were capable of using IHP as a sole source of C and P. From a further 238 ...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m97-073
更新日期：1997-06-01 00:00:00
abstract：:A simplified procedure is presented for releasing and concentrating indigenous microbial cells from soil for viewing by transmission electron microscopy as thin sections or replicas of frozen-etched preparations. This procedure is compared with two others reported earlier, and their relative merits are discussed as co...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m75-036
更新日期：1975-03-01 00:00:00
abstract：:The anaerobic degradative pathway for metabolism of 2,4,6-trinitrotoluene (TNT) by a consortium of Desulfovibrio spp. isolated from a creek sediment was studied. This consortium has the metabolic capability to degrade TNT to fatty acids. The growth of the consortium and the metabolism of TNT were greatly enhanced in t...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m96-155
更新日期：1996-12-01 00:00:00
abstract：:A bacterial strain capable of utilizing 3,6-dimethylphenanthrene (3,6-DMP) as its sole source of carbon and energy was isolated from a creosote-contaminated soil. The isolate was identified as a strain of Sphingomonas sp. and was designated strain JS1. Utilization of 3,6-DMP was demonstrated by an increase in bacteria...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w03-018
更新日期：2003-02-01 00:00:00
abstract：:Two rod-shaped viruses of similar length occurring in hops were isolated from a mixed infection. One (hop mosaic virus) infected Nicotiana clevelandii, produced mosaic symptoms in mosaic-sensitive hop cultivars, and did not infect Chenopodium murale. The other (hop latent virus) infected C. murale but not N. cleveland...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m76-168
更新日期：1976-08-01 00:00:00
abstract：:We present preliminary data on the role of antibiotics in intraabdominal sepsis using a new, clinically relevant animal model. Peritoneal cavity infection was induced by ligation and perforation of the cecum in adult rats. Surviving rats were randomized to receive either saline or cefoxitin at the time of cecal excisi...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m88-059
更新日期：1988-03-01 00:00:00
abstract：:Previously, we reported the metabolic responses of Pseudomonas sp. strain HF-1, a nicotine-degrading bacterium, to nicotine stress. However, the metabolic effects of nicotine on non-nicotine-degrading bacteria that dominate the environment are still unclear. Here, we have used nuclear magnetic resonance based metabolo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/cjm-2014-0206
更新日期：2014-08-01 00:00:00
abstract：:Dietary exposure to heavy metals may have detrimental effects on human and animal health, even at low concentrations. Specific probiotic bacteria may have properties that enable them to bind toxins from food and water. We assessed the interaction of probiotic bacteria with cadmium and lead in vitro as an initial scree...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w06-043
更新日期：2006-09-01 00:00:00
abstract：:Trichloroethylene (TCE) is a prevalent contaminant of groundwater that can be cometabolically degraded by indigenous microbes. Groundwater contaminated with TCE from a US Department of Energy site in Ohio was used to characterize the site-specific impact of phenol on the indigenous bacterial community for use as a pos...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/w05-025
更新日期：2005-06-01 00:00:00
abstract：:Cellobiose transport by the cellulolytic ruminal anaerobe Fibrobacter (Bacteroides) succinogenes was measured using randomly tritiated cellobiose. When assayed at the same concentration (1 mM), total cellobiose uptake was one-fourth to one-third that of total glucose uptake. The abilities of F. succinogenes to transpo...
journal_title：Canadian journal of microbiology
pub_type： 杂志文章
doi：10.1139/m91-021
更新日期：1991-02-01 00:00:00
