Abstract:
:Six effective Rhizobium leguminosarum bv. phaseoli strains were examined for nodulation competitiveness on common bean (Phaseolus vulgaris L.), using all possible two-strain combinations of inoculum. Nodule occupancy was determined with strain-specific fluorescent antibodies. The strains were divided into three groups according to their overall competitive abilities on pole bean cv. Kentucky Wonder and bush bean cv. Bountiful. Strains TAL 182 and TAL 1472 were highly competitive (greater than 70% nodule occupancy); strains KIM-5, Viking 1, and CIAT 899 were moderately competitive (approximately 50% nodule occupancy); and strain CIAT 632 was poorly competitive (less than 5% nodule occupancy). The competitiveness of the six strains was similar on the two host cultivars. The proportion of competing strains in the inoculum influenced the nodule occupancy of the highly competitive and moderately competitive strains, but not that of the poorly competitive strain. Two outstanding strains (TAL 182 and TAL 1472) were identified as ideal model strains for molecular and genetic studies on nodulation competitiveness.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
George ML,Robert FMdoi
10.1139/m92-026subject
Has Abstractpub_date
1992-02-01 00:00:00pages
157-60issue
2eissn
0008-4166issn
1480-3275journal_volume
38pub_type
杂志文章abstract::A real-time polymerase chain reaction assay that uses degenerate primers and a dual-labelled probe was developed to detect the bexA gene of Haemophilus influenzae, including those belonging to non-b serotypes as well as clonal division II strains. This assay is sensitive and specific, detecting 20 copies of the gene, ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0040
更新日期:2013-05-01 00:00:00
abstract::A mercuric ion-reducing flavoprotein was purified from Yersinia enterocolitica 138A14 using dye matrix affinity chromatography. The purified enzyme had a characteristic absorption spectrum similar to those of flavin compounds, and FAD was detected as a part of the purified enzyme by thin-layer chromatography. Freshly ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-027
更新日期:1993-02-01 00:00:00
abstract::We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial spec...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w06-102
更新日期:2007-01-01 00:00:00
abstract::Schizophyllum commune Fr. was shown, by light, scanning, and transmission electron microscopy, to be a destructive mycoparasite on several phytopathogenic and nematode-trapping fungi. The hyphae of S. commune coiled around host hyphae and fruiting structures and penetrated them by means of either unspecialized hyphae ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-131
更新日期:1978-07-01 00:00:00
abstract::This study aimed to examine aminoglycosides (AMGs) resistance mechanisms, including the AMG-modifying enzyme genes, mexXY, rplY, nuoG, and galU, in the Pseudomonas aeruginosa non-cystic fibrosis (CF) isolates in Thailand. One hundred P. aeruginosa isolates from non-CF patients were examined for susceptibility to AMGs ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0465
更新日期:2013-01-01 00:00:00
abstract::High resolution two-dimensional PAGE was used to analyse protein variation among serotype 1 poliovirus isolates. Viruses isolated from patients with recent histories of vaccination with live attenuated poliovirus were compared with prototype serotype 1 poliovaccine. The nonvaccine Mahoney and Brunenders strains of ser...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m88-136
更新日期:1988-06-01 00:00:00
abstract::The site specificity of metallic ion binding in Escherichia coli K-12 lipopolysaccharide was assessed by collecting high-resolution phosphorus nuclear magnetic resonance spectra in the presence of manganese, a paramagnetic divalent cation. This technique revealed high-affinity interactions between the cation and all o...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-010
更新日期:1986-01-01 00:00:00
abstract::Subtyping Neisseria meningitidis by methods that rely on monoclonal antibody (mAb) reactivity results in an unusually high number of strains that are not subtypeable. To subtype 48 strains isolated (1993-1994) in the province of Quebec that were not subtypeable by mAb-based techniques, we used DNA sequencing of the va...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Wild-type glucose uptake and glucose chemotaxis activities were restored in glucose transport defective Pseudomonas aeruginosa strains PFB360 and PFB362 after introduction of plasmid pPZ129, containing a 1.1-kilobase DNA fragment that is essential for the expression of the P. aeruginosa periplasmic glucose binding pro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m93-163
更新日期:1993-11-01 00:00:00
abstract::In both relA+ and relA- derivatives, the biosynthesis of peptidoglycan, lipid intermediates, and nucleotide precursors abruptly halted at the onset of diauxic lag from glucose to lactose with a concomitant accumulation of guanosine 5'-diphosphate 3'-diphosphate (ppGpp). These results are consistent with the proposal t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-188
更新日期:1979-10-01 00:00:00
abstract::From the four known isoforms of the staphylococcal exfoliative toxins (ETs), only ETA and ETB are the major causative agents. General knowledge is that the gene for ETA is located on the chromosome, whereas that for ETB is located on a large plasmid. Yoshizawa and co-workers (2000, Microbiol. Immunol. 44(3): 189-191) ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2012-0721
更新日期:2013-04-01 00:00:00
abstract::Enzymes induced by two Rhizobium trifolii bacteriophages caused depolymerization of exopolysaccharides from most R. trifolii and R. leguminosarum strains tested, but did not, in general, attack the exopolysaccharides of R. meliloti, the slow-growing rhizobia, or Agrobacterium. Ca2+ and (or) Mg2+ were required for enzy...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-239
更新日期:1975-10-01 00:00:00
abstract::Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and th...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-236
更新日期:1978-12-01 00:00:00
abstract::An intracellular invertase was induced in cultures of Clostridium pasteurianum utilizing sucrose as its carbon source for growth. This enzyme synthesis could be repressed by the addition of fructose of a sucrose-growing culture. In contrast, invertase activity was not affected by the addition of glucose to sucrose-gro...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m75-251
更新日期:1975-11-01 00:00:00
abstract::The purpose of this study was to examine antimicrobial activity of endophytic fungi isolated from the leaves, stems, and roots of 54 species of Orchidaceae collected in a Brazilian tropical ecosystem. In total, 382 filamentous fungi and 13 yeast isolates were obtained and cultured to examine the production of crude ex...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W09-101
更新日期:2009-12-01 00:00:00
abstract::The poly(3-hydroxybutyrate) (PHB) depolymerase structural gene of Comamonas sp. (phaZCsp) was cloned in Escherichia coli and identified by halo formation on PHB-containing solid medium. The nucleotide sequence of a 1719 base pair MboI fragment was determined and contained one large open reading frame (ORF1, 1542 base ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m95-183
更新日期:1995-01-01 00:00:00
abstract::For the recently described serotype 15 of biotype I and serotypes 13 and 14 of biotype II of Actinobacillus pleuropneumoniae, fhuA and hgbA were detected by polymerase chain reaction and DNA sequencing. To determine the substrate specificity of the iron receptors FhuA and HgbA and to study their role in the virulence ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w05-135
更新日期:2006-04-01 00:00:00
abstract::The phototrophic purple sulfur bacterium Thiopedia rosea forms multicellular, gas-vacuolate, regular, flat aggregates (platelets, sheets) held together by slime. Platelets found in eutrophic water consisted of slime (85% of the total wet volume) and 16 cells, while the gas-filled vacuole occupied 44% of the volume of ...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m86-113
更新日期:1986-07-01 00:00:00
abstract::An active in vitro protein-synthesizing system has been developed from Rhodospirillum rubrum grown under either photosynthetic or heterotrophic conditions. A protease activity has been found in both of these systems, and this activity can be readily inactivated by treating the cells with KCl and phenylmethyl sulfonylf...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m76-042
更新日期:1976-02-01 00:00:00
abstract::The gene-encoding HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus JCM2501 was amplified and expressed in Escherichia coli and the enzymatic features were examined. The enzyme was detected mainly in the membrane fraction. It catalyzed the sulfide-dependent menaquinone (MK) reduction showing special enz...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/W07-077
更新日期:2007-09-01 00:00:00
abstract::Dithiothreitol reacted, at pH 9.0, with the isolated cell walls of Methanospirillum hungatii, to release about 23% of the cell wall dry weight as a high molecular weight fraction (> 0.5 million daltons). Untreated walls consisted of 70% amino acids, 11% lipid, and 6.6% carbohydrate. Sugars were identified as rhamnose,...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-017
更新日期:1980-02-01 00:00:00
abstract::Two bacterial species, Gram-negative Escherichia coli and Gram-positive Bacillus subtilis, were exposed to different auxins to examine possible effects of these substances on bacterial stress tolerance. Bacterial resistance to UV irradiation, heat shock, and streptomycin was assessed with and without previous exposure...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2013-0266
更新日期:2013-11-01 00:00:00
abstract::The insect pathogenic fungus Aschersonia placenta is a highly effective pathogen of whiteflies and scale insects. However, few genetic tools are currently available for studying this organism. Here we report on the conditions for the production of transformable A. placenta protoplasts using an optimized protocol based...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/cjm-2015-0770
更新日期:2016-07-01 00:00:00
abstract::During growth on minimal salts - glucose media supplemented with high concentrations of Na2SO3 (10-3 and 10-2 M), Salmonella heidelberg exhibited cytological and growth responses which indicated increased cellular toxicity with increasing sulfite concentrations. The large quantities of sulfide evolved during growth at...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m79-217
更新日期:1979-12-01 00:00:00
abstract::Antibiotic-resistant and antimetabolite-resistant mutants of the nitrogen-fixing symbiotic bacterium Frankia were isolated to provide strains with genetic backgrounds amenable to genetic analysis. The lethal and mutagenic effects of ethyl methanesulfonate (EMS) and UV light on four Frankia strains were investigated. U...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w04-013
更新日期:2004-04-01 00:00:00
abstract::Neisseria gonorrhoeae strains can be grouped or differentiated (auxotyped) by their requirements for none, or any one or more of proline, uracil, hypoxanthine, and citrulline or ornithine. Most strains were readily assessed because they responded with growth or no growth on each defined auxanographic medium. Other str...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m83-204
更新日期:1983-10-01 00:00:00
abstract::Pyrenocine A, a phytotoxin produced by Pyrenochaeta terrestris (Hansen) Gorenz, Walker and Larson, possesses general antibiotic activity against plants, fungi, and bacteria. Effective doses for 50% inhibition (ED50s) are 4 micrograms/mL for onion seedling elongation; 14, 20, 20, and 25 micrograms/mL for the germinatio...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m87-055
更新日期:1987-04-01 00:00:00
abstract::Southern bean mosaic virus (SBMV) protein was cleaved with cyanogen bromide and a highly basic peptide, CB-1, was isolated by ion exclusion and ion-exchange chromatography. Twelve peptides were separated from a tryptic digest of CB-1 by ion-exchange chromatography and the composition of these peptides was similar to t...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m80-241
更新日期:1980-12-01 00:00:00
abstract::The addition of various concentrations of monensin (1,5, and 10 micrograms/mL) to the culture medium inhibits the fungal growth and perturbs exopolysaccharides secretion, provoking a decrease of production in Botrytis cinerea and an increase in Sclerotium rolfsii. The ionophore induces also modifications in both polym...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m96-124
更新日期:1996-09-01 00:00:00
abstract::Humicola lutea 103 is a copper-tolerant fungal strain able to grow in the presence of 300 μg·mL(-1) Cu(2+) under submerged cultivation. To prevent the consequences of copper overload, microorganisms have evolved molecular mechanisms that regulate its uptake, intracellular traffic, storage, and efflux. In spite of this...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w2012-112
更新日期:2012-12-01 00:00:00