Abstract:
:The site specificity of metallic ion binding in Escherichia coli K-12 lipopolysaccharide was assessed by collecting high-resolution phosphorus nuclear magnetic resonance spectra in the presence of manganese, a paramagnetic divalent cation. This technique revealed high-affinity interactions between the cation and all of the lipopolysaccharide phosphoryl groups. To ascertain whether the carboxyl groups of 2-keto-3-deoxyoctonate contributed to the metal cation binding, lipopolysaccharide was chemically modified using a glycine ethyl ester - carbodiimide reaction. Of the three available carboxyl groups, only one was neutralized by the exogenously added ligand; the others appeared to be cross-linked within the molecule. By analogy, only one carboxyl group should be freely available for binding metallic ions, while the others are probably neutralized by the close proximity of endogenous amino substituents. Although high-resolution phosphorus nuclear magnetic resonance showed that an intermolecular conformational change had occurred after the carboxyl groups were neutralized, titration with manganese revealed no differences in the apparent strength of the interactions between the cation and the phosphoryl groups. Together, these data suggest that the high affinity of lipopolysaccharide for divalent metallic ions can be attributed primarily to the phosphoryl substituents and not free carboxyl groups.
journal_name
Can J Microbioljournal_title
Canadian journal of microbiologyauthors
Ferris FG,Beveridge TJdoi
10.1139/m86-010subject
Has Abstractpub_date
1986-01-01 00:00:00pages
52-5issue
1eissn
0008-4166issn
1480-3275journal_volume
32pub_type
杂志文章abstract::Repetitive element-polymerase chain reaction (REP-PCR) DNA fingerprinting and library-based microbial source tracking (MST) methods were utilized to investigate the potential sources of Escherichia coli pollution in recreational waters of southeastern Lake Huron. In addition to traditional sources such as humans, agri...
journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/w08-123
更新日期:2009-03-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:1983-10-01 00:00:00
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journal_title:Canadian journal of microbiology
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更新日期:1990-11-01 00:00:00
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journal_title:Canadian journal of microbiology
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journal_title:Canadian journal of microbiology
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更新日期:2002-05-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
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更新日期:2007-11-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:10.1139/m78-204
更新日期:1978-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Canadian journal of microbiology
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更新日期:1999-06-01 00:00:00
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更新日期:1983-01-01 00:00:00
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更新日期:2009-08-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
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更新日期:1983-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1139/m76-007
更新日期:1976-01-01 00:00:00
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journal_title:Canadian journal of microbiology
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doi:10.1139/m75-036
更新日期:1975-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1139/m96-070
更新日期:1996-05-01 00:00:00
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journal_title:Canadian journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1979-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1977-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1980-06-01 00:00:00
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更新日期:1980-03-01 00:00:00