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Detection of viral DNA and E4 protein in basal keratinocytes of experimental canine oral papillomavirus lesions.

Abstract：

:We studied experimental canine oral papillomavirus (COPV) infection by in situ hybridization and immunohistochemistry of weekly biopsies. After 4 weeks, viral DNA in rete ridges suggested a keratinocyte stem cell target. Abundant viral DNA was seen in E4-positive cells only. E4 was predominantly cytoplasmic but also nuclear, being concentrated in the nucleoli during wart formation. Infected cells spread laterally along the basal layer and into the parabasal layers, accompanied by E7 transcription and increased mitoses. Most of the lower epithelium was positive for viral DNA, but, in mature warts, higher levels of E4 expression and genome amplification occurred in only sporadic superficial cells. L1 expression was late and in only a subset of E4-positive cells. During regression, viral DNA was less abundant in deep epithelial layers, suggesting downregulation of replication prior to replacement of infected cells from beneath. Detection of viral DNA in post-regression tissue indicated latent infection.

journal_name

Virology

journal_title

Virology

authors

Nicholls PK,Doorbar J,Moore RA,Peh W,Anderson DM,Stanley MA

doi

10.1006/viro.2001.0868

subject

Has Abstract

pub_date

2001-05-25 00:00:00

pages

82-98

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(01)90868-2

journal_volume

284

pub_type

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