Porcine reproductive and respiratory syndrome virus non-structural protein 1 suppresses tumor necrosis factor-alpha promoter activation by inhibiting NF-κB and Sp1.

Abstract:

:The objective of this study was to identify porcine reproductive and respiratory syndrome virus (PRRSV)-encoded proteins that are responsible for the inhibition of TNF-α expression and the mechanism(s) involved in this phenomenon. Using a TNF-α promoter reporter system, the non-structural protein 1 (Nsp1) was found to strongly suppress the TNF-α promoter activity. Such inhibition takes place especially at the promoter's proximal region. Both Nsp1α and Nsp1β, the two proteolytic fragments of Nsp1, were shown to be involved in TNF-α promoter suppression. Furthermore, using reporter plasmids specific for transcription factors (TFs) that bind to TNF-α promoter, Nsp1α and Nsp1β were demonstrated to inhibit the activity of the TFs that bind CRE-κB(3) and Sp1 elements respectively. Subsequent analyses showed that Nsp1α moderately inhibits NF-κB activation and that Nsp1β completely abrogates the Sp1 transactivation. These findings reveal one of the important mechanisms underlying the innate immune evasion by PRRSV during infection.

journal_name

Virology

journal_title

Virology

authors

Subramaniam S,Kwon B,Beura LK,Kuszynski CA,Pattnaik AK,Osorio FA

doi

10.1016/j.virol.2010.07.016

subject

Has Abstract

pub_date

2010-10-25 00:00:00

pages

270-9

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(10)00456-3

journal_volume

406

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • A conserved internal hydrophobic domain mediates the stable membrane integration of the dengue virus capsid protein.

    abstract::The mature flavivirus capsid protein (virion C) is commonly thought to be free in the cytoplasm of infected cells and to form a nucleocapsid-like complex with genomic RNA in mature virus particles. There is little sequence conservation among flavivirus virion C proteins, but they are similar in size (e.g., 99 amino ac...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1997.8608

    authors: Markoff L,Falgout B,Chang A

    更新日期:1997-06-23 00:00:00

  • Molecular characterization of a new porcine rotavirus P genotype found in an asymptomatic pig in Slovenia.

    abstract::Rotaviral RNA was detected in the stool sample of an asymptomatic fattening pig at a Slovenian pig farm. To characterize the rotavirus, RT-PCR was used, employing primers specific for the VP7, VP4 and NSP4 genes. Specific products were purified and the sequencing reaction was performed for the molecular analysis of am...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2006.10.001

    authors: Steyer A,Poljsak-Prijatelj M,Barlic-Maganja D,Jamnikar U,Mijovski JZ,Marin J

    更新日期:2007-03-15 00:00:00

  • Characterization of Toscana virus-defective interfering particles generated in vivo.

    abstract::Toscana (TOS) virus stocks strongly interfering with standard virus replication were obtained by sequential passages of virus in suckling mouse brain. Characterization of viral RNAs in these stocks showed the presence of a heterogeneous population of defective RNA molecules derived from the L genomic segment, in both ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1998.9195

    authors: Marchi A,Nicoletti L,Accardi L,Di Bonito P,Giorgi C

    更新日期:1998-06-20 00:00:00

  • The matrix protein of HIV-1 is not sufficient for assembly and release of virus-like particles.

    abstract::The matrix (MA) proteins of human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) are known to be important for the targeting and assembly of lentiviral proteins. The objective of the present study was to determine whether the MA protein of HIV-1 was sufficient for particle assembly and r...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1998.9284

    authors: Giddings AM,Ritter GD Jr,Mulligan MJ

    更新日期:1998-08-15 00:00:00

  • Homotypic interaction and multimerization of hepatitis C virus core protein.

    abstract::Hepatitis C virus (HCV) core protein constitutes a viral nucleocapsid and may possess multiple functions. In this study, we demonstrated the homotypic interaction and multimerization of HCV core protein in vitro and in vivo. By using a yeast two-hybrid system, we showed that the amino-terminal hydrophilic portion (ami...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0164

    authors: Matsumoto M,Hwang SB,Jeng KS,Zhu N,Lai MM

    更新日期:1996-04-01 00:00:00

  • Human hematopoietic (CD34+) stem cells possess high-affinity receptors for adenovirus type 11p.

    abstract::Gene transfer into human hematopoietic stem cells using Ad5 is inefficient due to lack of the primary receptor CAR and the secondary receptors alphavbeta3 integrin and alphavbeta5 integrin, and due to the high seroprevalence of Ad5 antibodies in most adults, resulting in diminished gene transduction. In the present st...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2004.07.018

    authors: Mei YF,Segerman A,Lindman K,Hörnsten P,Wahlin A,Wadell G

    更新日期:2004-10-25 00:00:00

  • Release of coronavirus E protein in membrane vesicles from virus-infected cells and E protein-expressing cells.

    abstract::Coronavirus E protein is a small viral envelope protein that plays an essential role in coronavirus assembly; coexpression of coronavirus M and E proteins results in the production of virus-like particles. The present study demonstrated that mouse hepatitis virus (MHV) E protein was released as an integral membrane pr...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1999.9955

    authors: Maeda J,Maeda A,Makino S

    更新日期:1999-10-25 00:00:00

  • Site-specific rearrangements in the long terminal repeat of extra mouse mammary tumor proviruses in murine T-cell leukemias.

    abstract::Extra MMTV proviruses in T-cell leukemias of GR and C57/BL10 mice contain alterations in their long terminal repeat (LTR) sequence. The four different leukemias studied contain different deletions, but common hallmarks were observed around the recombination sites. At the 5' end of the deletions we observed a common no...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(86)90431-9

    authors: Michalides R,Wagenaar E

    更新日期:1986-10-15 00:00:00

  • Identification of putative polymerase gene product in cells infected with murine coronavirus A59.

    abstract::The virion RNA of mouse hepatitis virus, strain A59 (MHV-A59) is believed to be the mRNA for the viral RNA-dependent RNA polymerase. The cell-free translation of virion RNA results in the synthesis of two predominant products p220 and p28 (M. R. Denison and S. Perlman, 1986, J. Virol. 60, 12-18). p28 is a basic protei...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(87)90303-5

    authors: Denison M,Perlman S

    更新日期:1987-04-01 00:00:00

  • In vitro processing of human immunodeficiency virus type 1 Gag virus-like particles.

    abstract::Human immunodeficiency virus (HIV) Gag proteins are assembled into virus particles and then cleaved by the virion-associated HIV protease. Concomitant with Gag processing, doughnut-like HIV particles (the immature form) are converted to particles containing condensed cores (the mature form). Here we describe the in vi...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.2000.0370

    authors: Morikawa Y,Shibuya M,Goto T,Sano K

    更新日期:2000-07-05 00:00:00

  • Umbravirus-encoded proteins both stabilize heterologous viral RNA and mediate its systemic movement in some plant species.

    abstract::The proteins encoded by open reading frame 3 (ORF3) of the umbraviruses pea enation mosaic virus-2 and tobacco mottle virus, like that of groundnut rosette virus, mediated the movement of viral RNA through the phloem of infected Nicotiana benthamiana or N. clevelandii plants when they were expressed from chimeric toba...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.2001.1078

    authors: Ryabov EV,Robinson DJ,Taliansky M

    更新日期:2001-09-30 00:00:00

  • Turnip crinkle virus infection from RNA synthesized in vitro.

    abstract::Genome-length cDNA clones of turnip crinkle virus (TCV) were constructed with SmaI and XbaI restriction sites engineered at the 5' and 3' termini, respectively. The genome-length cDNAs were positioned downstream of modified lambda and T7 phage promoters such that in vitro transcription resulted in RNAs with 5 extra nu...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(89)90368-1

    authors: Heaton LA,Carrington JC,Morris TJ

    更新日期:1989-05-01 00:00:00

  • Genetic diversity and purifying selection in West Nile virus populations are maintained during host switching.

    abstract::To investigate differential evolutionary rates and selective forces of WNV in hosts and vectors, we measured the genetic diversity that arose during alternating passage in mosquitoes and birds. Within-host genetic diversity was monitored in each of three experimentally passed replicates, and the complete genome sequen...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2008.02.032

    authors: Jerzak GV,Brown I,Shi PY,Kramer LD,Ebel GD

    更新日期:2008-05-10 00:00:00

  • Sequence analysis of the phosphoprotein (P) genes of human parainfluenza type 4A and 4B viruses and RNA editing at transcript of the P genes: the number of G residues added is imprecise.

    abstract::We cloned and sequenced the cDNAs against genomic RNAs and mRNAs for phosphoproteins (Ps) of human parainfluenza virus types 4A (PIV-4A) and 4B (PIV-4B). The PIV-4A and -4B P genes were 1535 nucleotides including poly(A) tract and were found to have two small open reading frames, neither of which was apparently large ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(90)90413-l

    authors: Kondo K,Bando H,Tsurudome M,Kawano M,Nishio M,Ito Y

    更新日期:1990-09-01 00:00:00

  • Specific restrictions in the progression of Venezuelan equine encephalitis virus-induced disease resulting from single amino acid changes in the glycoproteins.

    abstract::The pathogenesis of Venezuelan equine encephalitis virus (VEE) was examined in the mouse model using V3000, a virus derived from a molecular clone of the Trinidad donkey strain of VEE. These results were compared in parallel experiments with avirulent mutants of VEE derived by site-directed mutagenesis of the clone. A...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1995.1022

    authors: Grieder FB,Davis NL,Aronson JF,Charles PC,Sellon DC,Suzuki K,Johnston RE

    更新日期:1995-02-01 00:00:00

  • Functional expression of influenza A viral nucleoprotein in cells transformed with cloned DNA.

    abstract::Simian cells permissive for influenza A virus infection were stably transformed with a full-length cloned influenza A nucleoprotein gene under the control of an inducible metallothionein promoter and linked to a dihydrofolate reductase gene to facilitate cell selection. Transformed cells synthesized a virus-specific n...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(86)90437-x

    authors: Ryan KW,Mackow ER,Chanock RM,Lai CJ

    更新日期:1986-10-15 00:00:00

  • Equivalent immunogenicity of the highly attenuated poxvirus-based ALVAC-SIV and NYVAC-SIV vaccine candidates in SIVmac251-infected macaques.

    abstract::Therapeutic immunization of HIV-1-infected individuals may induce and/or enhance HIV-1-specific immune responses and decrease the dependency on antiretroviral drug treatment. However, repeated immunizations with live-recombinant vectors may induce vector-specific immune responses that interfere with the elicitation of...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.2002.1722

    authors: Hel Z,Nacsa J,Tsai WP,Thornton A,Giuliani L,Tartaglia J,Franchini G

    更新日期:2002-12-05 00:00:00

  • Enterovirus infections of the central nervous system.

    abstract::Enteroviruses (EV) frequently infect the central nervous system (CNS) and induce neurological diseases. Although the CNS is composed of many different cell types, the spectrum of tropism for each EV is considerable. These viruses have the ability to completely shut down host translational machinery and are considered ...

    journal_title:Virology

    pub_type: 杂志文章,评审

    doi:10.1016/j.virol.2010.12.014

    authors: Rhoades RE,Tabor-Godwin JM,Tsueng G,Feuer R

    更新日期:2011-03-15 00:00:00

  • Endocytosis-mediated HIV-1 entry and its significance in the elusive behavior of the virus in astrocytes.

    abstract::Astrocytes protect neurons but also evoke a proinflammatory response to injury and viral infections including HIV. We investigated the mechanism of HIV-1 infection in primary astrocytes, which showed minimal but productive viral infection independent of CXCR4. As with ectopic-CD4-expressing astrocytes, lysosomotropic ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2014.03.002

    authors: Chauhan A,Mehla R,Vijayakumar TS,Handy I

    更新日期:2014-05-01 00:00:00

  • Interferon-alpha-induced gene expression: evidence for a selective effect of ouabain on activation of the ISGF3 transcription complex.

    abstract::Binding of interferons (IFNs) to their cell surface receptors stimulates rapid translocation of cytoplasmic proteins to the nucleus and the expression of a variety of cellular genes within minutes. Translocated proteins subsequently bind to the interferon-stimulated response element (ISRE) located in the promoters of ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(92)91207-b

    authors: Nakagawa Y,Petricoin EF 3rd,Akai H,Grimley PM,Rupp B,Larner AC

    更新日期:1992-09-01 00:00:00

  • Inclusion of the NS2-specific exon in minute virus of mice mRNA is facilitated by an intronic splicing enhancer that affects definition of the downstream small intron.

    abstract::Alternative splicing of pre-mRNAs plays a critical role in maximizing the coding capacity of the small parvovirus genome. The small-intron region of minute virus of mice (MVM) pre-mRNAs undergoes an unusual pattern of overlapping alternative splicing, using two donors, D1 and D2, and two acceptors, A1 and A2, within a...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1999.9696

    authors: Haut DD,Pintel DJ

    更新日期:1999-05-25 00:00:00

  • Altered properties of the herpes simplex virus ICP8 DNA-binding protein in cells infected with ICP27 mutant viruses.

    abstract::Herpes simplex virus mutant strains that fail to encode a functional ICP27 polypeptide show a reduction in their ability to replicate viral DNA compared to wild-type virus. To define the nature of this defect, we examined the formation of viral DNA replication structures in the nucleus of cells infected with these mut...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1993.1449

    authors: Curtin KD,Knipe DM

    更新日期:1993-09-01 00:00:00

  • Vaccination with herpes simplex virus type 1 glycoprotein K impairs clearance of virus from the trigeminal ganglia resulting in chronic infection.

    abstract::Following primary ocular infection, HSV-1 establishes a latent infection in the trigeminal ganglia with the complete absence of detectable infectious virus. Recently, we showed that vaccination of BALB/cJ with HSV-1 glycoprotein K (gK), but not other HSV-1 glycoproteins, significantly exacerbated the severity of HSV-1...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0537

    authors: Ghiasi H,Cai S,Nesburn AB,Wechsler SL

    更新日期:1996-10-01 00:00:00

  • Influenza virus is not restricted by tetherin whereas influenza VLP production is restricted by tetherin.

    abstract::Tetherin (ST2/CD317) is a cellular protein that restricts the release from cells of some enveloped viruses including HIV-1. To examine if influenza virus is affected by tetherin, MDCK cells constitutively expressing human tetherin and control MDCK cells were infected with influenza virus. No difference was observed in...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2011.05.006

    authors: Watanabe R,Leser GP,Lamb RA

    更新日期:2011-08-15 00:00:00

  • Evidence for a continued requirement for CD40/CD40 ligand (CD154) interactions in the progression of LP-BM5 retrovirus-induced murine AIDS.

    abstract::In genetically susceptible C57BL/6 mice the LP-BM5 isolate of murine retroviruses causes profound splenomegaly, lymphadenopathy, hypergammaglobulinemia, and an immunodeficiency syndrome bearing many similarities to the pathologies seen in AIDS. Because of these similarities, which also include terminal B cell lymphoma...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1997.8970

    authors: Green KA,Noelle RJ,Green WR

    更新日期:1998-02-15 00:00:00

  • Immunological detection of proteins associated with the Epstein-Barr virus nuclear antigen 2A.

    abstract::The Epstein-Barr virus nuclear antigen 2A (EBNA-2A) has been strongly implicated in the EBV-mediated B-cell transformation process. Since EBNA-2A might exert this function through interaction with proteins of the infected cell, we studied the association of EBNA-2A with cellular proteins. Immunoprecipitation of EBNA-2...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1993.1406

    authors: Grässer FA,Sauder C,Haiss P,Hille A,König S,Göttel S,Kremmer E,Leinenbach HP,Zeppezauer M,Mueller-Lantzsch N

    更新日期:1993-08-01 00:00:00

  • The RING finger domain of the varicella-zoster virus open reading frame 61 protein is required for its transregulatory functions.

    abstract::Varicella-zoster virus (VZV) open reading frame 61 (ORF61) protein transactivates expression of VZV promoters. VZV ORF61 is functionally homologous to herpes simplex virus type 1 (HSV-1)-infected cell protein 0 (ICP0); however, amino acid sequence homology of these two proteins is limited to the RING finger domains, a...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1639

    authors: Moriuchi H,Moriuchi M,Cohen JI

    更新日期:1994-11-15 00:00:00

  • Glycan-dependent chikungunya viral infection divulged by antiviral activity of NAG specific chi-like lectin.

    abstract::Highly pathogenic alphaviruses display complex glycans on their surface. These glycans play a crucial role in viral pathogenesis by facilitating glycan-host interaction during viral entry which can be targeted. Various studies have reported antiviral activity of lectins that bind to the glycans present on the surface ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2018.10.009

    authors: Kaur R,Neetu,Mudgal R,Jose J,Kumar P,Tomar S

    更新日期:2019-01-02 00:00:00

  • CCAAT/enhancer binding protein beta binds to and activates the P670 promoter of human papillomavirus type 16.

    abstract::The P670 promoter of HPV16 directs transcription of the virus late genes in the differentiating epithelium. We found that CCAAT/enhancer binding protein beta (C/EBPbeta), a key transcription factor that induces the terminal differentiation of keratinocytes, enhanced the P670-driven transcription in transient reporter ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2005.10.025

    authors: Kukimoto I,Takeuchi T,Kanda T

    更新日期:2006-03-01 00:00:00

  • Lack of LTR and ENV genetic variation during bovine leukemia virus-induced leukemogenesis.

    abstract::Genetic variation of the Bovine Leukemia Virus (BLV) appears to be limited in vitro and during the latent phase of the disease. However, cells in tumors often harbor deleted proviruses that are defective for expression. In order to gain insight into the involvement of viral genetic variation during pathogenesis, the B...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/s0042-6822(95)80007-7

    authors: Willems L,Kerkhofs P,Burny A,Mammerickx M,Kettmann R

    更新日期:1995-01-10 00:00:00