Abstract:
:The objective of this study was to identify porcine reproductive and respiratory syndrome virus (PRRSV)-encoded proteins that are responsible for the inhibition of TNF-α expression and the mechanism(s) involved in this phenomenon. Using a TNF-α promoter reporter system, the non-structural protein 1 (Nsp1) was found to strongly suppress the TNF-α promoter activity. Such inhibition takes place especially at the promoter's proximal region. Both Nsp1α and Nsp1β, the two proteolytic fragments of Nsp1, were shown to be involved in TNF-α promoter suppression. Furthermore, using reporter plasmids specific for transcription factors (TFs) that bind to TNF-α promoter, Nsp1α and Nsp1β were demonstrated to inhibit the activity of the TFs that bind CRE-κB(3) and Sp1 elements respectively. Subsequent analyses showed that Nsp1α moderately inhibits NF-κB activation and that Nsp1β completely abrogates the Sp1 transactivation. These findings reveal one of the important mechanisms underlying the innate immune evasion by PRRSV during infection.
journal_name
Virologyjournal_title
Virologyauthors
Subramaniam S,Kwon B,Beura LK,Kuszynski CA,Pattnaik AK,Osorio FAdoi
10.1016/j.virol.2010.07.016subject
Has Abstractpub_date
2010-10-25 00:00:00pages
270-9issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(10)00456-3journal_volume
406pub_type
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