Abstract:
:Twelve avian reovirus strain 176 proteins, 10 structural (lambda 1, lambda 2, lambda 3, mu 1, mu 2/mu 2C, sigma 1, sigma 2, sigma 3, and sigma 4), and 2 nonstructural (mu NS and sigma NS) were identified and characterized by electrophoretic analysis of purified virions and infected cell lysates. Three of the identified proteins (mu 1, mu NS, and sigma 4) have not been previously described. In pulse-chase experiments, 10 of the proteins (lambda 1, lambda 2, lambda 3, mu 1, mu 2, mu NS, sigma 1, sigma 2, sigma NS, sigma 3) were shown to be primary translation products, whereas mu 2C was shown to be a post-translational cleavage product of mu 2.
journal_name
Virologyjournal_title
Virologyauthors
Ni Y,Ramig RF,Kemp MCdoi
10.1006/viro.1993.1147subject
Has Abstractpub_date
1993-03-01 00:00:00pages
466-9issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(83)71147-5journal_volume
193pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Central African tropical forests are home to several species of non-human primates (NHPs), infected by Simian Immunodeficiency Virus (SIV). It is well-known that HIV-1 epidemic is due to cross-transmission and adaptation of SIV to humans. The main goal of this work was to investigate if a NHP bite is a risk factor for...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.12.026
更新日期:2017-03-01 00:00:00
abstract::Groundnut bud necrosis virus induces necrotic symptoms in different hosts. Previous studies showed reactive oxygen species-mediated programmed cell death (PCD) resulted in necrotic symptoms. Transgenic expression of viral protein NSs mimics viral symptoms. Here, we showed a role for NSs in influencing oxidative burst ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.05.003
更新日期:2017-08-01 00:00:00
abstract::The budded form of Autographa californica nuclear polyhedrosis virus functionally enters cells by adsorptive endocytosis. During the period of virus uptake into Spodoptera frugiperda IPLB Sf21 cells (1 to 4 hr postinfection), filamentous F-actin cables, visualized by fluorescence microscopy, were formed within the cyt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1585
更新日期:1993-11-01 00:00:00
abstract::Loss of T cell homeostasis usually precedes the onset of AIDS. We hypothesized that rapid progressors may be transmitted with HIV-1 that is particularly able to perturb T cell homeostasis. To this end, we have tested two transmitted, syncytium-inducing (SI) viral isolates from a rapid progressor in two thymus models. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.07.019
更新日期:2004-10-10 00:00:00
abstract::BS-1, a stroma cell line derived from normal human bone marrow, can support the growth of murine erythroid (BFU-E), granulocyte-macrophage (CFU-GM), and megakaryocyte (CFU-Meg) progenitor cells in a short-term in vitro coculture system. Exposure of BS-1 cell to the human immunodeficiency virus (HIV) prior to coculture...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1159
更新日期:1993-03-01 00:00:00
abstract::Terminally redundant linear proviral DNA of approximately 9.5 kb was the major unintegrated species recovered in the Hirt supernatant fraction of caprine synovial membrane cells infected with strain 75-G63 caprine arthritis-encephalitis virus. A physical map based on the cleavage sites of 13 restriction endonucleases ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90318-0
更新日期:1984-04-30 00:00:00
abstract::Little is known about the neutralization properties of HIV-1 in India to optimally design and test vaccines. For this reason, a functional Env clone was obtained from each of ten newly acquired, heterosexually transmitted HIV-1 infections in Pune, Maharashtra. These clones formed a phylogenetically distinct genetic li...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.12.032
更新日期:2009-03-15 00:00:00
abstract::The current antiretroviral therapy (ART) can effectively reduce plasma HIV loads to undetectable levels, but cannot eliminate latently infected resting memory CD4 T cells that persist for the lifetime of infected patients. Therefore, designing new therapeutic approaches to eliminate these latently infected cells or th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.08.002
更新日期:2013-11-01 00:00:00
abstract::Mice inoculated with replication-competent stocks of the murine acquired immunodeficiency syndrome (MAIDS) virus are severely immunocompromised and proned to the development of T- and B-cell lymphomas. We have studied the development of T-cell lymphomas in C57BL/6 and RF/J mice inoculated with helper-free stocks of th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80072-7
更新日期:1995-01-10 00:00:00
abstract::Hematopoietic tissues obtained from avian leukosis virus (ALV)-infected Hyline SC chickens were analyzed for the presence of integrated viral DNA sequences. Cells were prepared from bone marrow, bursa, spleen, thymus, and peripheral blood. Following the removal of erythrocytes, cellular DNAs from each of these tissues...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90216-3
更新日期:1986-12-01 00:00:00
abstract::Cauliflower mosaic virus (CaMV) uses a specialised translation mechanism to bypass the long leader sequence of the 35S RNA. The effect of the CaMV 35S RNA leader sequence on the expression of a downstream beta-glucuronidase (GUS) reporter gene was studied in transgenic tobacco plants. Enzymatic GUS assays of these tra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9038
更新日期:1998-03-15 00:00:00
abstract::Replication of the nonsegmented, plus-stranded RNA genome of Cucumber necrosis tombusvirus (CNV) requires two essential overlapping viral-coded replication proteins, the p33 replication co-factor and the p92 RNA-dependent RNA polymerase. In this paper, we demonstrate that p33 is phosphorylated in vivo and in vitro by ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.08.006
更新日期:2005-12-05 00:00:00
abstract::Vaccinia virus DNA ligase has been expressed in Escherichia coli, purified, and biochemically characterized. The enzyme ligates double-stranded (ds) DNA substrates with either cohesive or blunt-end termini and the latter reaction is stimulated by PEG. Vaccinia virus DNA ligase can also ligate oligo(dT) when annealed t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0358
更新日期:1996-07-01 00:00:00
abstract::N-tropic murine leukemia viruses have been observed in connection with radiation-induced osteosarcomagenesis in BALB/c mice. We have investigated the bone disease-inducing potential of molecularly cloned, BALB/c-derived N-tropic viruses in the random-bred NMRI mouse strain. The germ-line virus and an exogenous virus i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90171-m
更新日期:1990-12-01 00:00:00
abstract::Red sea bream iridovirus (RSIV) of the Iridoviridae family is a causative agent of lethal infections in many cultured marine fish species in southwestern Japan. RSIV-induced apoptosis was divided as follows: (1). cell shrinkage and rounding at the early apoptotic stage, (2). cell enlargement at the middle apoptotic st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.02.006
更新日期:2004-05-01 00:00:00
abstract::Cucumber mosaic virus (CMV) is a damaging pathogen of over 200 mono- and dicotyledonous crop species worldwide. It has the broadest known host range of any virus, but the timescale of its evolution is unknown. To investigate the evolutionary history of this virus, we obtained the genomic sequences of 40 CMV isolates f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.09.024
更新日期:2016-01-01 00:00:00
abstract::Mutants of the influenza virus A/seal/Mass/1/80 (H7N7) are described which contain an insertion of 60 nucleotides in the hemagglutinin (HA) gene, derived most probably by recombination between the HA gene and the nucleoprotein gene of the same virus. The nonhomologous RNA recombination resulted in an enhanced hemagglu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1555
更新日期:1994-10-01 00:00:00
abstract::Binding of simian virus 40 (SV40) large T antigen to human and calf thymus topoisomerase I (topo I) was readily detected by using modified enzyme-linked immunosorbent assays and immunoblots. In addition to WT T antigen, binding could also be readily demonstrated with T antigen fragments from the amino-terminal region ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0433
更新日期:1996-08-15 00:00:00
abstract::Hepatitis B viruses replicate via reverse transcription of an RNA intermediate. This RNA pregenome serves as mRNA and is packaged into capsids and reverse transcribed. Both processes require the interaction of the viral reverse transcriptase, P protein, with the 5'-proximal epsilon-signals on the pregenome. For epsilo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8329
更新日期:1997-01-20 00:00:00
abstract::Infection with Epstein-Barr virus (EBV) usually leads to a latent state in B lymphocytes. The virus can be reactivated through two viral transactivators, Zta and Rta, leading to a cascade of gene expression. An EBV DNA array was generated to analyze the pattern of transcription of the entire EBV genome under various c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.09.064
更新日期:2006-02-20 00:00:00
abstract::Dengue virus (DENV) and West Nile virus (WNV) are members of the Flavivirus genus of positive-strand RNA viruses. RNA sequences and structures, primarily in the untranslated regions, have been shown to modulate flaviviral gene expression and genome replication. Previously, we demonstrated that a structure in the DENV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.034
更新日期:2008-09-30 00:00:00
abstract::Feline immunodeficiency virus (FIV) is a lentivirus associated with an immunodeficiency syndrome of the domestic cat. A short open reading frame (ORF2), of unknown function, is present in all FIV isolates. We have investigated the role of ORF2 in determining the cell tropism of two infectious molecular clones of FIV. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0002
更新日期:1996-01-01 00:00:00
abstract::In a previous study, we observed that hepatitis C virus (HCV) core protein specifically inhibits translation initiated by an HCV internal ribosome entry site (IRES). To investigate the mechanism by which down-regulation of HCV translation occurs, a series of mutations were introduced into the IRES element, as well as ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.013
更新日期:2006-02-20 00:00:00
abstract::Tombusviruses, of which tomato bushy stunt virus (TBSV) is the type member, spontaneously generate defective interfering RNAs (DIs) that are known to interfere with viral accumulation and symptom development. We show that co-infection with TBSV and DIs causes a dramatic reduction in accumulation of TBSV subgenomic RNA...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1410
更新日期:1995-08-01 00:00:00
abstract::There is growing evidence that the receptor-binding characteristics of influenza viruses are affected by the host-dependent glycosylation of viral hemagglutinin (HA). To better understand these effects, we propagated two variants of the human influenza virus USSR/90/77 (which differed by the mutation Asn131 reversible...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9224
更新日期:1998-08-01 00:00:00
abstract::While the 2009 pandemic H1N1 virus has become established in the human population as a seasonal influenza virus, continued adaptation may alter viral virulence. Here, we passaged a 2009 pandemic H1N1 virus (A/Changchun/01/2009) in mice. Serial passage in mice generated viral variants with increased virulence. Adapted ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.09.014
更新日期:2015-12-01 00:00:00
abstract::Since SARS-CoV-2 spreads rapidly around the world, data have been needed on the natural fluctuation of viral load and clinical indicators associated with it. We measured and compared viral loads of SARS-CoV-2 from pharyngeal swab, IgM anti-SARS-CoV-2, CRP and SAA from serum of 114 COVID-19 patients on admission. Posit...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.04.011
更新日期:2020-07-01 00:00:00
abstract::The immediate-early transcripts of pseudorabies virus have been located in a region of the genome situated internally within the inverted repeat between map positions 0.99 and 0.95. A single immediate-early transcript (approximately 6 kb) can be detected both in the cytoplasmic and nuclear fractions of infected, cyclo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90510-x
更新日期:1983-12-01 00:00:00
abstract::Encephalitis is a rare, but serious complication from vaccination against smallpox using replication competent strains of vaccinia virus. In this report we describe mutants of vaccinia virus, containing N-terminal deletions of the vaccinia virus interferon resistance gene, E3L, that are attenuated for neuropathogenesi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.006
更新日期:2005-03-15 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) glycoproteins K and B (gK and gB) are intimately involved in virus-induced fusion of cells. Certain mutations in the UL27 (gB) and UL53 (gK) genes confer a syncytial (syn) phenotype characterized by extensive fusion of infected cells and giving rise to multinucleated cells. We have ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1507
更新日期:1993-10-01 00:00:00